Cpd (NM_012836) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213267 representing NM_012836 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGGCTGGGATGAGCGGCCGCCCTGGCGGCTGGAAAGCCTGCGGCTGCTACCGCCGCCACCGC TGCTGCTGCTACTGCTGCTATTGAGGAGCTCGGCCCAGGCGGCGCACATCAAGAAGGCTGAGGCGACCAC GACGACCGTCGGCGGCTCCGAGGCGGCCGAGGGCCAGTTTGACCACTACTACCACGAAGCGGCGCTGGGC GAGGCACTGGAGGCGGCAGCGGCCGCGGGGCCGCCTGGCCTGGCCCGTCTCTTCAGCATCGGCAATTCGG TGGAGGGCCGACCGCTGTGGGTGCTGCGCCTCACGGCTGGCCTGGGGCCGCCGCCCACCCCCGCCGCCGT GGGGCTGGACGCCGCGGGGCCGCTGCTGCCCGGCCGACCGCAGGTGAAGCTGGTGGGTAACATGCACGGC GACGAGACGGTGTCGCGCCAGGTGTTGGTCTACCTGGCCCGTGAGCTGGCGTCGGGCTACCGCCGCGGGG ACCCGCGCCTGGTCCGCCTGCTCAACACCACCGACGTATATTTGCTGCCCAGCCTGAATCCGGATGGCTT CGAACGCGCTCGCGAGGGTGACTGCGGCCTCGGCGACAGCGGTCCGCCTGGGACCAGCGGCCGAGACAAC AGCCGAGGCCGCGACCTCAACCGCAGCTTCCCTGACCAGTTCAGCACGGGGGAGCCACCTTCCCTGGATG AGGTGCCAGAGGTGCGCGCCCTCATCGACTGGATCCGCAGGAACAAGTTTGTGCTTTCTGGAAATCTTCA TGGTGGCTCAGTGGTAGCAAGCTATCCTTTTGATGATTCTCCCGAGCATAAGACCACTGGAATCTATAGC AAAACCTCCGATGATGAAGTCTTTCGATACTTGGCGAAAGCTTATGCTTCTAACCACCCTATAATGAGAA CTGGTGAACCCCATTGTCCAGGAGATGAAGAGGAGACTTTCAAAGACGGGATCACGAACGGTGCGCACTG GTATGATGTGGAAGGTGGTATGCAAGATTACAACTATGTGTGGGCCAACTGTTTTGAGATCACATTAGAA TTATCTTGCTGCAAATACCCACCTGCTTCTCAGCTTCGACAAGAATGGGAGAACAACCGTGAGTCTTTGA TCACATTGATTGAAAAGGTCCACATTGGAATTAAAGGATTTGTTAAAGACTCAGTCACAGGCTCTGGGTT GGAGAATGCAACCATCTCAGTGGCTGGTATTAATCATAATATCACAACTGGGAGATTCGGTGATTTCCAC AGACTGCTCATTCCTGGAAGTTACAATCTTACAGCGGTTTCACCTGGGTATATGCCGTTGACTATTAATA ATATAGTGGTGAAAGAAGGACCAGCCACAGAGATAGATTTCTCCCTTCAACCAACTGTGATGTCAGTAGT GCCTGACTCGACTGAGGCTGTGACAACTCCCGGCACAGTTGCCGTTCCTAATATTCCTCCTGGAACACCA TCGTCCCATCAGCCAATTCAGCCTAAAGACTTTCACCACCACCATTTTCCTGACATGGAAATCTTCTTGA GAAGATTTGCCAATGAATATCCTAACATCACTCGCCTTTATTCTCTGGGAAAGTCCGTAGAGTCGAGAGA ACTGTATGTGATGGAGATATCTGACAACCCAGGCGTCCATGAACCAGGTGAACCAGAATTTAAATATATT GGAAATATGCATGGAAATGAAGTGGTTGGAAGAGAACTGCTATTGAATCTCATCGAATACCTCTGCAAGA ACTTTGGAACAGACCCTGAAGTAACAGATTTGGTTCGCAGCACTAGAATTCACCTCATGCCATCCATGAA TCCTGATGGGTATGAGAAGTCCCAAGAAGGAGACTCGATAAGTGTAGTTGGCAGAAACAACAGCAACAAC TTTGACCTAAACCGAAATTTCCCAGACCAGTTTGTTCCGATCACAGATCCTACCCAACCTGAAACTATCG CTGTGATGAGCTGGGTGAAGGCCTATCCGTTTGTGCTGTCGGCCAATCTGCATGGAGGTTCCTTGGTGGT TAACTACCCTTATGATGATAATGAACAAGGAGTTGCCACATACAGCAAATCACCAGATGACGCCGTGTTT CAACAAATAGCACTTTCTTATTCCAAGGAAAACTCGCAGATGTTCCAAGGTAGACCTTGCAAAGACATGT ACCTTAATGAGTATTTTCCTCATGGCATAACGAATGGAGCGAGTTGGTATAATGTCCCAGGTGGTATGCA GGACTGGAACTATTTACAGACGAATTGCTTTGAAGTAACCATCGAACTAGGTTGTGTGAAATATCCATTC GAGAAGGAACTGCCAAAGTACTGGGAGCAAAACCGGAGATCTCTAATCCAGTTTATGAAACAGGTTCATC AGGGTGTCAAAGGATTTGTTCTTGATGCCACAGATGGTAGGGGCATATTAAATGCCACCCTCAGTGTTGC TGAAATTAATCACCCAGTGACGACTTACAAAGCTGGAGATTACTGGCGTCTCTTGGTCCCAGGGACATAT AAAATCACAGCATCTGCTCGAGGGTATAACCCCGTCACCAAGAACGTGACCGTCAGGAGTGAAGGTGCTA TTCAGGTCAACTTCACACTTGTTCGATCTTCAACAGATGCAAACAATGAGTCAAAGAAAGGAAAGGGGCA TAGCACCAGCACTGATGACACCAGCGACCCAACTTCTAAGGAGTTTGAAGCACTGATCAAACACCTTTCG GCTGAGAATGGCTTGGAAGGCTTCATGTTAAGCTCGTCCTCAGACCTGGCTCTGTATCGATACCATTCCT ACAAAGACTTATCCGAGTTCCTGAGAGGACTCGTAATGAACTACCCACACATTACAAACCTTACCACTTT GGGACAGAGTGTTGAATATCGTCACATTTGGTCCCTTGAAATCTCCAATAAGCCCAACATATCAGAACCT GAAGAACCGAAGATTCGTTTTGTTGCTGGTATCCATGGAAACGCTCCAGTTGGCACTGAACTGCTTTTGG CTCTGGCAGAATTTCTTTGCCTGAATTACAAAAAGAATCCAGTTGTTACCCAATTGGTTGACAGGACTAG AATTGTGATTGTCCCCTCTCTGAATCCAGATGGGAGAGAGCGAGCACAGGAGAAAGACTGTACCTCGAAG ACTGGGCACACAAATGCCCGTGGCAGGGACCTGGACACAGACTTCACAAGTAATGCTTCCCAACCTGAGA CCAAAGCCATCATTGAAAACTTGATTCAAAAACAGGATTTCAGCCTTTCCATTGCTTTAGATGGTGGTTC TGTGCTGGTCACATATCCGTACGACAAGCCAGTCCAGACAGTGGAAAACAAAGAAACGTTAAAGCATCTG GCGTCTCTTTATGCAAATAACCACCCATCAATGCACATGGGTCAGCCCAGTTGCCCAAATAATTCAGATG AGAACATACCAGGAGGAGTGATGCGGGGAGCAGAGTGGCACAGTCACCTGGGCAGCATGAAGGATTATAG TGTCACCTATGGCCATTGCCCAGAAATCACAGTATACACAAGCTGCTGCTACTTCCCAAGTGCAGCTCAA CTCCCTGCCCTATGGGCAGAGAACAAGAAGTCTCTCCTCAGCATGCTGGTGGAGGTCCACAAAGGAGTCC ATGGGTTAGTAAAAGACAAGACTGGTAAACCAATCTCTAAAGCAGTAATTGTACTCAATGAAGGAATAAG AGTACACACAAAAGAGGGGGGTTATTTCCATGTGCTTTTAGCTCCTGGGGTCCATAACATCAATGCCATC GCTGATGGATATCAGCAACAGCATTCACAGGTCTTTGTGCATCATGATGCAGCCAGTTCTGTGGTGATAG TCTTTGATACAGATAACCGGATATTTGGTTTGCCAAGGGAACTTGTGGTAACTGTGTCAGGTGCCACCAT GTCAGCGCTGATCCTCACGGCTTGCATCATTTGGTGCATCTGCTCTATCAAGTCTAACAGACACAAAGAT GGCTTCCACCGGCTGCGGCAGCATCATGATGAATATGAAGATGAAATTCGCATGATGTCAACTGGCTCTA AGAAGTCCCTTTTAAGCCATGAGTTCCAGGATGAAACGGACACAGAGGAAGAGACATTATATTCTAGCAA ACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213267 representing NM_012836 Red=Cloning site Green=Tags(s) MASGWDERPPWRLESLRLLPPPPLLLLLLLLRSSAQAAHIKKAEATTTTVGGSEAAEGQFDHYYHEAALG EALEAAAAAGPPGLARLFSIGNSVEGRPLWVLRLTAGLGPPPTPAAVGLDAAGPLLPGRPQVKLVGNMHG DETVSRQVLVYLARELASGYRRGDPRLVRLLNTTDVYLLPSLNPDGFERAREGDCGLGDSGPPGTSGRDN SRGRDLNRSFPDQFSTGEPPSLDEVPEVRALIDWIRRNKFVLSGNLHGGSVVASYPFDDSPEHKTTGIYS KTSDDEVFRYLAKAYASNHPIMRTGEPHCPGDEEETFKDGITNGAHWYDVEGGMQDYNYVWANCFEITLE LSCCKYPPASQLRQEWENNRESLITLIEKVHIGIKGFVKDSVTGSGLENATISVAGINHNITTGRFGDFH RLLIPGSYNLTAVSPGYMPLTINNIVVKEGPATEIDFSLQPTVMSVVPDSTEAVTTPGTVAVPNIPPGTP SSHQPIQPKDFHHHHFPDMEIFLRRFANEYPNITRLYSLGKSVESRELYVMEISDNPGVHEPGEPEFKYI GNMHGNEVVGRELLLNLIEYLCKNFGTDPEVTDLVRSTRIHLMPSMNPDGYEKSQEGDSISVVGRNNSNN FDLNRNFPDQFVPITDPTQPETIAVMSWVKAYPFVLSANLHGGSLVVNYPYDDNEQGVATYSKSPDDAVF QQIALSYSKENSQMFQGRPCKDMYLNEYFPHGITNGASWYNVPGGMQDWNYLQTNCFEVTIELGCVKYPF EKELPKYWEQNRRSLIQFMKQVHQGVKGFVLDATDGRGILNATLSVAEINHPVTTYKAGDYWRLLVPGTY KITASARGYNPVTKNVTVRSEGAIQVNFTLVRSSTDANNESKKGKGHSTSTDDTSDPTSKEFEALIKHLS AENGLEGFMLSSSSDLALYRYHSYKDLSEFLRGLVMNYPHITNLTTLGQSVEYRHIWSLEISNKPNISEP EEPKIRFVAGIHGNAPVGTELLLALAEFLCLNYKKNPVVTQLVDRTRIVIVPSLNPDGRERAQEKDCTSK TGHTNARGRDLDTDFTSNASQPETKAIIENLIQKQDFSLSIALDGGSVLVTYPYDKPVQTVENKETLKHL ASLYANNHPSMHMGQPSCPNNSDENIPGGVMRGAEWHSHLGSMKDYSVTYGHCPEITVYTSCCYFPSAAQ LPALWAENKKSLLSMLVEVHKGVHGLVKDKTGKPISKAVIVLNEGIRVHTKEGGYFHVLLAPGVHNINAI ADGYQQQHSQVFVHHDAASSVVIVFDTDNRIFGLPRELVVTVSGATMSALILTACIIWCICSIKSNRHKD GFHRLRQHHDEYEDEIRMMSTGSKKSLLSHEFQDETDTEEETLYSSKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012836
ORF Size	4134 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012836.2, NP_036968.1
RefSeq Size	4374 bp
RefSeq ORF	4137 bp
Locus ID	25306
UniProt ID	Q9JHW1
MW	152.6 kDa
Gene Summary	putative carboxypeptidase D protein that may participate in peptide processing in the secretory pathway; alternatively spliced CPD-N form is induced by prolactin and is localized to the nucleus [RGD, Feb 2006]

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