Cdc42bpa (NM_053657) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mrck; Pk428
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213192 representing NM_053657 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGAGAAGTGCGTTTGAGGCAGTTGGAACAGTTTATTTTAGATGGGCCAGCTCAGACCAATGGGC AGTGCTTCAGTGTGGAGACGTTACTGGACATACTCATCTGTCTTTATGATGAATGCAATAATTCTCCATT GAGAAGAGAGAAGAACATTCTTGAATATCTAGAATGGGCTAAACCATTCACTTCTAAAGTGAAGCAAATG CGCTTACATAGAGAAGACTTCGAAATATTAAAGGTGATTGGTCGAGGAGCTTTTGGTGAGGTTGCTGTAG TTAAACTGAAAAATGCAGATAAAGTATTTGCCATGAAAATTCTGAACAAGTGGGAAATGCTGAAAAGAGC TGAGACAGCGTGCTTTCGTGAAGAGAGGGATGTGTTGGTAAATGGAGACAGTAAGTGGATAACCACTCTG CACTATGCTTTTCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTACTTA CTCTGCTCAGCAAATTTGAAGATCGATTGCCAGAAGAGATGGCTCGGTTTTACTTGGCTGAGATGGTGAT AGCCATTGACTCAGTTCACCAGCTCCACTATGTTCACAGAGACATCAAACCTGACAATATATTGATGGAT ATGAATGGACATATTCGTTTAGCAGATTTTGGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTCCAGT CCTCAGTGGCAGTTGGAACTCCAGACTACATTTCCCCGGAAATCCTTCAGGCTATGGAGGACGGGAAAGG CAGATATGGACCAGAGTGTGACTGGTGGTCCTTGGGAGTCTGCATGTATGAGATGCTTTACGGAGAAACA CCATTCTATGCGGAATCTCTGGTGGAGACATATGGAAAAATTATGAATCATAAAGAGAGGTTTCAGTTTC CAACCCAAGTGACTGATGTGTCTGAAAATGCTAAAGATCTTATTCGAAGACTCATTTGTAGCAGAGAACA TCGACTTGGCCAGAATGGAATAGAAGACTTTAAGAAACATCCGTTTTTCAGTGGAATTGATTGGGATAAC ATCCGAAACTGTGAAGCACCTTACATTCCAGAAGTTAGTAGTCCAACAGACACGTCAAATTTCGATGTGG ATGACGACTGTTTAAAAAACTCTGAAACAATGCCCCCACCAACACATACTGCATTTTCTGGCCATCATCT ACCATTTGTTGGGTTTACATATACTAGTAGCTGTGTTCTTTCTGATCGGAGCTGTTTAAGAGTCACAGCA GGTCCCACCTCACTGGATCTTGACGTCAATGTCCAGAGGACTCTAGATAATAACTTAGCAACTGAAGCTT ATGAAAGAAGAATTAAACGGCTAGAACAGGAAAAGCTTGAACTTACTAGAAAACTTCAAGAGTCAACACA GACTGTTCAAGCCCTGCAGTATTCAACTGTTGATGGTCCACTAACGGCAAGCAAAGATTTAGAAATTAAA AGTCTGAAAGAAGAGATTGAAAAACTAAGAAAACAAGTAGCAGAAGTGAATCATTTGGAACAACAACTTG AAGAAGCTAATTCTGTGAGACGAGAACTAGATGATGCTTTTAGGCAAATCAAAGCTTTTGAAAAACAAAT CAAAACTTTGCAGCAGGAAAGAGAAGAGCTAAATAAGGAACTAGTCCAGGCTAGTGAGCGATTAAAAAAC CAATCCAAAGAGCTGAAAGACGCACACTGTCAGAGGAAACTGGCCATGCAGGAGTTCATGGAGATCAATG AGCGACTAACAGAATTGCACACCCAAAAGCAGAAACTTGCTCGCCATGTCCGAGATAAGGAAGAAGAGGT GGACCTGGTGATGCAAAAAGCTGAGAGCTTAAGGCAGGAGCTTCGCAGAGCAGAAAGAGCCAAGAAAGAG CTGGAGGTTCACACTGAAGCTCTAATTGCTGAAGCCTCAAAGGACCGGAAGCTCCGAGAGCAGAGCAGAC ATTATTCTAAACAGCTGGAAAATGAATTGGAGGGACTGAAGCAAAAACAAATTAGTTACTCACCAGGAAT ATGCAGCATAGAACACCAGCAAGAGATAACTAAACTGAAAACTGACTTGGAAAAGAAAAGTATCTTTTAT GAAGAAGAAATCTCTAAAAGAGAAGGAATTCATGCAAGTGAGATTAAAAATCTGAAGAAGGAGCTGCATG ACTCAGAAGGCCAGCAACTTGCTCTCAACAAAGAAATTATGGTTTTAAAAGACAAATTGGAAAAAACCAG AAGGGAAAGTCAAAGTGAAAGGGAAGAATTTGAAAATGAGTTCAAGCAACAATATGAACGGGAAAAAGTA TTATTAACTGAAGAGAATAAAAAGTTAACGAGTGAACTCGATAAGCTAACTTCGTTGTATGAGAGCTTAA GTTTGCGCAACCAGCACTTAGAAGAAGAGGTCAAAGATCTAGCAGATAAGAAGGAATCAGTTGCCCACTG GGAAGCCCAAATCACAGAGATAATCCAATGGGTGAGTGATGAGAAGGACGCACGGGGCTATCTTCAGGCC TTAGCTTCTAAAATGACTGAAGAATTAGAAGCGTTAAGGAATTCCAGCCTGGGGACACGAGCGACAGATA TGCCCTGGAAAATGCGTCGTTTTGCAAAACTGGACATGTCAGCTAGACTAGAGTTGCAATCTGCCCTGGA TGCAGAAATCAGAGCTAAGCAGGCCATCCAGGAAGAGCTGAATAAAGTCAAAGCATCCAACATCATAACA GAATGTAAACTGAAAGATTCAGAGAAGAAGAACTTGGAACTGTTGTCCGAAATCGAGCAGCTGATAAAGG ACACTGAAGAGCTTAGATCTGAAAAGGGTGTAGAGCACCGAGACTCACAGCACTCTTTCTTGGCATTTTT GAATACGCCTACCGATGCTCTGGATCAATTTGAACGCTCTCCGTCCTGTACTCCAGCTGGCAAAGGCAGA CGTATTGCAGACTCTGCTCCGCTTCCAGTTCACACACCAACTTTAAGGAAAAAGGGATGCCCAGCTTCAG CTGGTTTTCCACCTAAGCGCAAGACTCATCAGTTTTTCGTGAAGTCTTTCACTGCCCCTACCAAGTGCCA TCAGTGTACCTCCTTGATGGTCGGTTTGATAAGACAGGGCTGTTCCTGCGAAGTCTGTGGGTTCTCATGT CATATAACTTGTGTGAACAAAGCCCCGACCACTTGCCCAGTTCCTCCTGAGCAAACTAAAGGCCCACTCG GTATAGACCCCCAGAAGGGAGTAGGGACAGCGTATGAGGGCCATGTCCGGATCCCTAAGCCGGCGGGAGT GAAGAAAGGGTGGCAGAGAGCACTGGCTGTGGTCTGTGACTTCAAACTGTTTCTTTATGATATCGCGGAG GGAAAAGCATCGCAGCCCAGCTCCGTCATTAGTCAAGTGATCGACATGAGAGATGAAGAATTTTCCGTGA GTTCAGTCTTGGCTTCTGATGTTATTCATGCAAGTCGAAAAGATATTCCCTGTATATTTAGAGTCACAGC CTCCCAGCTCTCAGCACCTAGTGACAAATGTTCCATCCTGATGCTAGCCGACAGCGAGACTGAGAGGAGT AAGTGGGTGGGAGTGCTGAGTGAACTCCACAAGGTTCTGAAGAAAAACAAGTTCAGAGACCGCTCAGTGT ATGTCCCCAAAGAGGCTTACGACAGCACTCTACCCCTCATTAAAACAACGCAGGCCGCGGCAATCATAGA TCATGAGCGGGTAGCTTTGGGAAATGAAGAAGGATTATTTGTTGTGCATGTCACTAAAGATGAGATTATT AGAGTTGGTGATAATAAGAAAATTCATCAGATTGAACTCATTCCAAGTGACCAGCTTGTTGCTGTGATCT CAGGCCGAAATCGCCACGTCCGACTTTTCCCTATGTCAGCTTTGGATGGGCGAGAGACGGATTTTTATAA ACTGGCAGAGACTAAAGGGTGTCAAACCATAGCTGCTGGGAAGGTGCGCCATGGAGCTCTGTCCTGCCTG TGTGTGGCCATGAAAAGACAGGTCCTCTGCTATGAACTGTTCCAAAGCAAGACCAGGCACAGGAAGTTTA AGGAGATTCAAGTCCCATGTAATGTCCAGTGGATGGCCATCTTCAGTGAACATCTCTGTGTGGGATTCCA GTCAGGATTTCTCAGATACCCCCTGAATGGGGAAGGAAGTCCATGCAATATGCTCCATTCAAATGACCAC ACACTGGCATTCATTACACACCAACCAATGGATGCTATCTGTGCCGTTGAGATCTCCAACAAAGAGTATT TGCTGTGTTTTAGCAGCATTGGGATATACACGGACTGCCAGGGCCGAAGATCTAGACAGCAGGAACTGAT GTGGCCAGCAAATCCTTCCTCCTGTTGTTACAATGCACCATATCTCTCCATTTACAGTGAAAATGCAGTT GATATCTTTGATGTAAACTCCATGGAATGGATACAGACGCTTCCTCTCAAGAAGGTTCGACCTTTAAACA CTGAAGGATCATTAAATCTTTTAGGGTTGGAGACCATTAGATTAATATATTTCAAAAATAAGATGGCAGA AGGGGATGAACTTGTAGTTCCTGAAACATCAGATAATAGCCGAAAACAGATGGTTAGAAACATCAACAAC AAACGGCGTTACTCCTTCCGGGTCCCAGAAGAAGAGAGGATGCAGCAGAGAAGGGAGATGCTACGAGATC CAGAAATGAGAAATAAATTAATTTCTAACCCAACTAATTTTAACCACATAGCACACATGGGTCCTGGAGA TGGAATACAGATCCTAAAAGACCTGCCCATGAACCCACGGCCTCAGGAAAGCCGGACAGTATTCAGTGGC TCCGTCAGTATTCCCTCCATCACCAAGTCCCGCCCTGAGCCAGGCCGCTCCATGAGTGCCAGCAGTGGCC TGTCTGCACGGTCATCAGCCCAGAATGGCAGTGCGCTAAAGAGGGAATTCTCTGGAGGAAGCTACAACAC GAAGCGACAGCCCATGCCCTCTCCCTCAGAAGGCTCTTTGTCATCAGGAGGCGTGGACCAAGGAAGTGAC GCCCCCGTGAGGGACTATGATGGAGAGGACTCTGATTCTCCAAGGCATTCCACAGCTTCCAACAGCTCTA ACCTGAGCAGCCCCCCAAGCCCCGTTTCACCCCGAAAGACCAAGAGCCTCTCCTTGGAGAGCACAGACCG TGGGAGCTGGGATCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213192 representing NM_053657 Red=Cloning site Green=Tags(s) MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQM RLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTL HYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMD MNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGET PFYAESLVETYGKIMNHKERFQFPTQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDN IRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTA GPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIK SLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKAFEKQIKTLQQEREELNKELVQASERLKN QSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKE LEVHTEALIAEASKDRKLREQSRHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFY EEEISKREGIHASEIKNLKKELHDSEGQQLALNKEIMVLKDKLEKTRRESQSEREEFENEFKQQYEREKV LLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQA LASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIIT ECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGVEHRDSQHSFLAFLNTPTDALDQFERSPSCTPAGKGR RIADSAPLPVHTPTLRKKGCPASAGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSC HITCVNKAPTTCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAE GKASQPSSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSDKCSILMLADSETERS KWVGVLSELHKVLKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERVALGNEEGLFVVHVTKDEII RVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCL CVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGSPCNMLHSNDH TLAFITHQPMDAICAVEISNKEYLLCFSSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSIYSENAV DIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINN KRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSG SVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGVDQGSD APVRDYDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRGSWDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053657
ORF Size	5196 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053657.1, NP_446109.1
RefSeq Size	6335 bp
RefSeq ORF	5199 bp
Locus ID	114116
UniProt ID	O54874
MW	197.1 kDa
Gene Summary	catalyzes the phosphorylation of Ser19 of nonmuscle myosin light chain; binds GTP-Cdc42 and plays a role in cytoskeleton organization [RGD, Feb 2006]

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