Cabin1 (NM_053575) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cain
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213176 representing NM_053575 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGAATTGCAGCCTTAAATGCCAGCTCCACAATTGAAGATGACCATGAAGGAAGCTTTAAAAGTC ACAAAATCCAGACAAAGGAGGCTCAGGAAGCAGAAGCCTTTGCCCTATACCACAAGGCCCTGGATCTACA GAAACATGACAGGTTTGAGGAGTCTGCCAAGGCCTACCATGAACTCCTGGAGGCTCGCCTGCTGCGAGAG GCAGTTTCATCCGGAGATGAGAAGGAAGGGCTGAAGCACCCTGGACTGATACTGAAATATTCTACGTATA AGAACCTGGCCCAGCTGGCAGCCCAGCGGGAGGACCTGGAGACAGCCATGGAGTTTTATTTGGAGGCCGT GATGCTGGATTCCACAGACGTCAACCTTTGGTACAAGATTGGACATGTGGCCCTGAGGCTCATCCGACTT CCCCTGGCTCGCCATGCTTTTGAGGAAGGGCTCCGGTGTAATCCTGACCACTGGCCCTGCTTGGACAACC TCATCACTGTTTTGTACACCCTCAGCGATTACACAACATGTCTTTACTTCATCTGCAAAGCACTGGAGAA AGACTGCCGCTACAGCAAAGGCCTGGTTCTCAAGGAGAAGATTTTTGAAGAGCAACCTTGTCTCCGAAAG GACTCACTCCGAATGTTCCTCAAATGTGACATGTCAGTTCACGAGGTGTCAGTGAATGCAGCAGAGACAC AGGCCATTGTAGACGAGGCTCTGGGGCTGCGGAAGAAGAGGCAGGCGTTGATCGTGCGGGAAAAGGAGCC AGACCTGAAACTAGTGCAGCCCATCCCTTTCTTCACGTGGAAGTGCCTTGGAGAGAGCTTGCTTGCCATG TACAATCACCTCACCACGTGTGAGCCTCCCAGGCCCAGCCTGGGCAAGAGGATTGACCTGTCTGACTACC AGGATCCCAGCCAGCTGCTAGCCCCCTCCGTCGTGGTTACTCCTGTCAGTGTGGTCCAGCCAAGTCCTGT TTGCACCAATCCAACTGTTGCTGTGGCCGAGCCTGTGCTCTCCTACACCTCTGTGACCACAACCAGCTTC CCACTGCACAGTCCTGGCCTGCTGGACACGGGCACTCCTATGGGTGATGTTTCCGGAGGAGATAAATCTA AAAAAGGGGTGAAGCGTAAGAAGACTTTAGAGGAAAGTGGGGAGACAGCAAAGCGGAGGTCTGCCCGTGT CCGCAACACCAAGTGCAAAAAAGAAGAGAAGGTGGATTTCCAGGGGCTTCTGGTAAAGTTCCTGCCGTCC AGGTTAAGAAAACTGGACCCTGAGGAGGAAGATGATCCATTTAACAACTATGAAGTTCAGTCAGAAGCCA AACTGGAGAGCTTCTCGAACGTGGGACCTCATCGACTGTCCTTCGATTCAGCCACATTCATGGAATCTGA GAAGCAGGATGTGCATGCGTTCCTGATGGAGAACCTGACCAATGGGGGCGTCCTGGAGCTCATGATGCGC TACCTGAAGAGCATGGGCCACAAGTTCCTGCTGAAGTGGCCCCCAGGCCTGGCGGAGGTCGTACTTAGCG TCTACCACAGCTGGAGGAGGCACAGTACCAGCCTGCCCAACCCATTGCTGAGGGACTGCAGCAACAAGCA CATCAAGGACATGATGCTGATGTCTCTTTCCTGCATGGAGCTCCAACTGGACCAGTGGCTTCTGACCAAA GGCAGAAGCTCCACGGTGTCTCCCCGGAACTGCCCTGCTGGTGTGGTGACAGGCAGGTTTGGGCCAGACT TCCCAGGAACCCACTGCCTGGGTGACCTCCTGCAGCTGTCATTTGCGTCATCTCAGCGGGATCTGTTTGA GGACGGCTGGCTGGAGTTTGTGGTCCGTGTTTACTGGCTGAAGGCACGTTTCTTGGCACTACAGGGCGAT ATGGAGCAAGCTTTGGAGAACTATGATATCTGCACAGAGATACTCCAGAGCTCCACGGCTCTCCAAGCCC AGGCAGGGGCTGAGCAAAGGGACATTGTCATCCGGCTGCCCAACCTGCACAATGACTCCATTGTTTCTCT GGAGGAAATTGATAAGAACCTGAAGTCTCTGGAAAGGTGCCAGTCCCTGGAGGAGATTCAGCGGCTGTTT GAGGCGGGCGACTACAAGGCTGTGGTGCAGCTGCTCCGGCCCACTCTGTGCACCAGTGGCTTTGACCGAG CCAAACACCTGGAGTTTATGACTTCCATTCCCGAGAGGCCGGCCCAGCTGCTTCTGCTGCAGGACTCTCT GCTCCGGCTGGAGGAGCACAGGCAGTGCTTTGAATGCTCTGACGTGGCCCTCAATGAAGCTGTCCAGCAG ATGCTGAACTCGAGTGACTCTGCGGCCAAGGAGGAGTGGGCGGCCACAGTGACGCAGCTGCTGCTAGGCA TGGAGCAGGCACTGTCATCAGACAGCAGGGGCAGCATCCTGAAGGAGTCCTCCTCTCCCACGGGCCTTGT CCGGCTCACCAACAACCTCATTCAGGTCATCGACTGCAGCATGGCTGTGCAGGAGGAGCCCAAGGAGCCC TATGTCTCCTCCGTGCTGCCCTGGATCATCCTCCACAGGATCATCTGGCAGGAGGAAGACACTTTCCGGT CTCTGTGCCACCAGCAGCAGCTCCAGAACCCAACGGAAGAAGGGATCTCGGAGATGCCCATGCTCCCATC TTCCCTCATGCTGCTCAACACAGCACATGAGTACCTGGGCAGGAGGTCCTGGTGCTGCAATTCAGATGGA GCGCTGCTGCGATTCTATGTGCACGTTCTCCAAAAGGAGCTTGCTGCGTCTGCCTCTGAGGACACTCACC CCTACAAGGAGGAGCTGGAGACCGCCTTGGAGCAGTGTTTCTACTGCCTCTACAGCTTCCCCAGCAAGAA GAGCAAGGCCAGGTACCTGGAGGAGCACTCAGCCCAGCAGGTGGACCTCACCTGGGAGGACGCCCTGTTC ATGTTTGAGTACTTCAAGCCCAAGACCCTCCCTGAGTTTGACAGCTACAAGACCAGCACCGTCTCCGCGG ACTTGGCAAACCTGTTGAAAAGAATCGCCACCATTGTGCCGCGCACAGAGAAGCCCGCCCTGAGCATGGA CAAAGTCTCTGCCTACATTGAGGGAACCTCAGCGGAGGTGCCCTGCCTTCCTGACGGGGCAGACCCCGCC CCTCCAGTGCTGAATGAGCTCTACTACCTCCTGGCTGACTATCACTTCAAAAACAAAGAGCAGTCCAAAG CCATCAAGTTCTACATGCATGACATTTGCATCTGCCCTAACAGGTTTGATTCCTGGGCAGGCATGGCTCT GGCCCGGGCCAGCCGAATTCAGGACAAGCTGAACTCAAATGAGCTGAAGAGTGATGGACCCATCTGGAGA CATGCCACACCTGTTCTGAACTGCTTCCGGAGGGCCCTTGAGATTGACAGCTCTAACTTGTCCTTGTGGA TTGAGTATGGCACCATGTCCTATGCCTTGCACTCATTCGCGTCCCGTCAGCTGAAGCAGTGGCGAGCCGA GCTGCCTCCTGAGGTTGTGCAGCAGATGGAGGATCGGCGAGACAGCATGCTGGAGACAGCCAGGCACTGT TTCACGTCTGCAGCCCACTGCGAGGGTGATGGCGATGAGGAGGAGTGGCTCATTCACTACATGCTGGGCA AAGTGGCCGAGAAGCAGCAGCAGCCGCCGACCGTGTACCTGCTGCACTACCGCCAGGCGGGCCACTACTT ACACGAGGAGGCCGCCCGCTACCCTAAGAAGATTCACTACCACAACCCGCCCGAGCTGGCCATGGAAGCC CTGGAGGTGTACTTCCGGCTCCATGCGTCCATCCTGAAGCTCCTGGGCAAACCTGACTCTGGGGTTAGCG CAGAGGTCCTGGTCAGCTTTATGAAGGAAGCAGCAGAAGGACCTTTTGCCAGGGGCGAGGAGAAGAATAC ACCTAAAGCTTCAGAAAAGGAGAAGGCCTGCCTGGTGGATGAGGACTCCCACTCTTCAGCTGGGACACTG CCGGGCCCCGGAGCCTCCCTTCCCTCCTCCTCTGGCCCAGGTCTGACATCCCCACCTTACACAGCCACTC CGATTGACCACGATTACGTCAAATGTAAAAAACCCCGTCAGCAGGCGACGCCGGACGACCGAAGCCAGGA CAGCACAGCTGTGGCACTGTCAGACTCCAGCTCAACACAGGACTTCTTTAATGAGCCCACAAGTTTGCTG GACGGTTCTCGAAAGCTCCTTCCAGAGAAGAGGATCTCTGGGCTCAGTGCTCAAGCAGGACCAAGTGGTA AAGACCTTCCAGGGCCCACAGAGGAAAGAGGAAAAACGGAGGAGTCTTTGGAGAGCACAGAGGCCTTCCG GGTTGTAGAACCAAGCGTCCAGAAGCCTGTGGCAGATTCCTCGGCCTCTGCTTACATCCCCAGCAAGCCC GCAGTGTCCACGCCCCCTCCGTGGGATGGGAAGAAGAGGAGTGACCCCCTAGGGGAGCCAGTGGCTTTCC CTCAGGGGCTGCCAGCAGGTGCTGAGGAACAGCGCCAGTTCCTCACAGAGCAGTGCATCGCCTCCTTCTG CCTGTGCTTGAGTCGCTTCCCACAGCACTACAAGAGTCTCTACCGGCTGGCCTTTCTCTACACCTACAGC AAGACTCACCGGAACCTCCAGTGGGCCCGTGATGTGTTGCTAGGCAGCAGTATCCCATGGCAACAACTGC AGCACATGCCGGCACAGGGGCTCTTCTGCGAGAGGAACAAGACCAATTTCTTCAACGGCATCTGGCGGAT CCCCGTGGATGAGATTGACCGGCCCGGCAGCTTCGCCTGGCACATGAACCGCTCCATCGTGCTGCTACTC AAGGTTCTAGCCCAGCTCCGGGACCACAGCACCCTGTTGAAGGTGTCCTCCATGCTGCAGCGGACACCAG ACCAGGGCAAGAAATATCTGCGAGATGCTGACCGCCAGGTGCTGGCACAGCGCGCCTTCATCCTCACTGT GAAGGTGCTGGAGGACACACTGAGTGAGCTTGCAGAGGGGTTAGAGCACCCAGGATCCAAGGCCTGTGGC CTCTCTGGAGCCAGGATGACCACCGATGTCTCACACAAAGCCAGTCCCGAGGATGGCCAGGAAAGCCTCC CACACCCCAAGAAGCTACCTCTAGCTGATGGCTCAGGACCAGGGCCTGAGCCAGGGGGCAAAGTGGGCCC TCTCCACCAATTGCCAGTGGCCACAGACACAAGGGACAACACAGAGCAGGGTGGGGAGCCGAAAGACAAA GAGCGTCCCCCGGTGGGGCCCACAGAACCCATGGACACCGGCGAGACTGCTGCTCGCCACCCAGACTTGG AACCGACGCCACGTCTGTTGCCAGGCCGCCCCCCAAGGGACCGGGGTCCCGAGAGCCGCTCTGCTGAGCT TTCCCTGGAGGAGCTGAGCATCAGCACCCGGCAGCAGCCTGCCCCACTTGTACCATCCCCAGTAACCCCC ACCACAGCTGCTCCCACTACCATGGGGGCCAGGGCAGCGGGGCACCCCGAGGAGGCACCGCCAAGGCCTA ATCGAAAGAGGAAACTCCTGCAGGACACAGAGTCTGGCAAGACGCTCCTTTTGGACGCCTACCGTGTGTG GCAGCAGGGCCAGAAGGCCATGGCCTATGACCTGAGCCGCATCGAGAAGATCATGTCGGAGACTTACATG CTCATCAAGCAGGTGGACGAGGAGACTGCACTGGAGCAGGCTGTGAAGTTCTGTCAGGTCCACCTCGGGG CTGCTGCCCAGAGACAGGCCTCAGGAGATGCACCCACTACCCCCAAGCACCCCAAGGACAGCCGAGAGAA TTTCTTCCCTGCAACAGTGGCCCCCTCAGCACCTGACACCACAGCACCTGACGCTCTCCAGCGGCCCAGT GACTCCCACCTCAAGCCTGGGTTGGCTGCTGCCATCACCTGCCCTCCCTCAGCATCAGCCTCTACCCCAG ACCCTGGAATTCCCCAGCCACACAGGCCAGAGGCTGTGCCCAGCAGGGCCCCTCTGAGTCCTGATGGAGA AGAGGTGTCAGGAGTGACAGAGGGTCCCAGCTTTCTATCCCAAGAACCACGGCACAGTCACCAGATGAAA ATGGCTGCCACTGGTCCCCTGGCCGAGCAGCACTGCTGGCCAGTGGAGGCTGCTTGCCAGACAGGTGCCG AGCCCACCTTCAGCCAAGCCACCAGCACCAAGGTCCCCAGCAGTGGAAGTACCCAGACACCTGAGAGCCA TCAAGGCAAAACTGAGTCCAGCAGAGCCAAGTCCCGCCTCCTGCCCAACATGCCAAAGCTGGTGATCCCC TCGGCCACCACCAAGTTCCCCCCTGAGATCACCGTCACACCACCTACCCCCACCCTGCTCTCCCCCAAAG GCAGCATCTCAGAGGAGACCAAGCAGAAGCTGAAGTCTGCCATCCTGTCTGCCCAATCTGCCGCCAATGT GAGGAAGGAGAGCCTGTGCCAGCCAGCCCTGGAGGTTCTGGAGACATCCAGCCAGGAGTCCTCATTGGAG AGTGAGACAGACGAGGACGATGACTTTATGGACGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213176 representing NM_053575 Red=Cloning site Green=Tags(s) MIRIAALNASSTIEDDHEGSFKSHKIQTKEAQEAEAFALYHKALDLQKHDRFEESAKAYHELLEARLLRE AVSSGDEKEGLKHPGLILKYSTYKNLAQLAAQREDLETAMEFYLEAVMLDSTDVNLWYKIGHVALRLIRL PLARHAFEEGLRCNPDHWPCLDNLITVLYTLSDYTTCLYFICKALEKDCRYSKGLVLKEKIFEEQPCLRK DSLRMFLKCDMSVHEVSVNAAETQAIVDEALGLRKKRQALIVREKEPDLKLVQPIPFFTWKCLGESLLAM YNHLTTCEPPRPSLGKRIDLSDYQDPSQLLAPSVVVTPVSVVQPSPVCTNPTVAVAEPVLSYTSVTTTSF PLHSPGLLDTGTPMGDVSGGDKSKKGVKRKKTLEESGETAKRRSARVRNTKCKKEEKVDFQGLLVKFLPS RLRKLDPEEEDDPFNNYEVQSEAKLESFSNVGPHRLSFDSATFMESEKQDVHAFLMENLTNGGVLELMMR YLKSMGHKFLLKWPPGLAEVVLSVYHSWRRHSTSLPNPLLRDCSNKHIKDMMLMSLSCMELQLDQWLLTK GRSSTVSPRNCPAGVVTGRFGPDFPGTHCLGDLLQLSFASSQRDLFEDGWLEFVVRVYWLKARFLALQGD MEQALENYDICTEILQSSTALQAQAGAEQRDIVIRLPNLHNDSIVSLEEIDKNLKSLERCQSLEEIQRLF EAGDYKAVVQLLRPTLCTSGFDRAKHLEFMTSIPERPAQLLLLQDSLLRLEEHRQCFECSDVALNEAVQQ MLNSSDSAAKEEWAATVTQLLLGMEQALSSDSRGSILKESSSPTGLVRLTNNLIQVIDCSMAVQEEPKEP YVSSVLPWIILHRIIWQEEDTFRSLCHQQQLQNPTEEGISEMPMLPSSLMLLNTAHEYLGRRSWCCNSDG ALLRFYVHVLQKELAASASEDTHPYKEELETALEQCFYCLYSFPSKKSKARYLEEHSAQQVDLTWEDALF MFEYFKPKTLPEFDSYKTSTVSADLANLLKRIATIVPRTEKPALSMDKVSAYIEGTSAEVPCLPDGADPA PPVLNELYYLLADYHFKNKEQSKAIKFYMHDICICPNRFDSWAGMALARASRIQDKLNSNELKSDGPIWR HATPVLNCFRRALEIDSSNLSLWIEYGTMSYALHSFASRQLKQWRAELPPEVVQQMEDRRDSMLETARHC FTSAAHCEGDGDEEEWLIHYMLGKVAEKQQQPPTVYLLHYRQAGHYLHEEAARYPKKIHYHNPPELAMEA LEVYFRLHASILKLLGKPDSGVSAEVLVSFMKEAAEGPFARGEEKNTPKASEKEKACLVDEDSHSSAGTL PGPGASLPSSSGPGLTSPPYTATPIDHDYVKCKKPRQQATPDDRSQDSTAVALSDSSSTQDFFNEPTSLL DGSRKLLPEKRISGLSAQAGPSGKDLPGPTEERGKTEESLESTEAFRVVEPSVQKPVADSSASAYIPSKP AVSTPPPWDGKKRSDPLGEPVAFPQGLPAGAEEQRQFLTEQCIASFCLCLSRFPQHYKSLYRLAFLYTYS KTHRNLQWARDVLLGSSIPWQQLQHMPAQGLFCERNKTNFFNGIWRIPVDEIDRPGSFAWHMNRSIVLLL KVLAQLRDHSTLLKVSSMLQRTPDQGKKYLRDADRQVLAQRAFILTVKVLEDTLSELAEGLEHPGSKACG LSGARMTTDVSHKASPEDGQESLPHPKKLPLADGSGPGPEPGGKVGPLHQLPVATDTRDNTEQGGEPKDK ERPPVGPTEPMDTGETAARHPDLEPTPRLLPGRPPRDRGPESRSAELSLEELSISTRQQPAPLVPSPVTP TTAAPTTMGARAAGHPEEAPPRPNRKRKLLQDTESGKTLLLDAYRVWQQGQKAMAYDLSRIEKIMSETYM LIKQVDEETALEQAVKFCQVHLGAAAQRQASGDAPTTPKHPKDSRENFFPATVAPSAPDTTAPDALQRPS DSHLKPGLAAAITCPPSASASTPDPGIPQPHRPEAVPSRAPLSPDGEEVSGVTEGPSFLSQEPRHSHQMK MAATGPLAEQHCWPVEAACQTGAEPTFSQATSTKVPSSGSTQTPESHQGKTESSRAKSRLLPNMPKLVIP SATTKFPPEITVTPPTPTLLSPKGSISEETKQKLKSAILSAQSAANVRKESLCQPALEVLETSSQESSLE SETDEDDDFMDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053575
ORF Size	6546 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053575.2, NP_446027.2
RefSeq Size	7260 bp
RefSeq ORF	6549 bp
Locus ID	94165
UniProt ID	O88480
MW	242.8 kDa
Gene Summary	physiologic inhibitor of calcineurin [RGD, Feb 2006]

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