Caskin2 (NM_001107065) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213130 representing NM_001107065 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGTGAACAGGACCTTATCCTTGCTGTCAAGAATGGAGACGTGACCTGCGTGCAGAAGCTGGTGG CTAAAGTGAAGGCTGCAAAAACAAAGCTCCTTGGCTCCACGAAGAGACTCAACATCAACTACCAGGATGC CGATGGATTCTCTGCCCTCCACCATGCTGCCTTGGGGGGCAGCCTGGAGCTCATAGCCTTGCTGCTGGAG GCTCAAGCCACTGTGGACATCAAGGACAGCAATGGCATGCGTCCCCTACACTATGCGGCCTGGCAGGGCC GGCTGGAGCCTGTGAAGCTGCTGCTGAGGGCGTCCGCGGCTGTCAATGCTGCCTCTCTGGACGGTCAGAT CCCTCTGCACCTGGCTGCACAGTATGGGCATTATGAGGTGTCAGAGATGCTTCTCCAGCACCAGTCTAAC CCGTGCCTAGTCAACAAGTTGAAGAAGACACCCCTAGACCTAGCCTGTGAATTTGGACGGCTCAAGGTGG CCCAGCTGCTTTTGAACAGCCACTTATGTGTGGCATTGCTGGAGGGAGAGGCAAAGGACCCATGTGACCC CAACTACACCACACCCCTGCACTTGGCTGCCAAGAATGGCCACAGAGAAGTCATCAGGCAGCTCTTGAAA GCCGGTATTGAGATCAACCGCCAGACCAAGACCGGCACTGCCCTCCACGAGGCCGCGCTCTACGGCAAAA CTGAGGTGGTGCGGCTGCTGCTGGAGGGAGGTGTGGATGTGAATATCCGGAACACATATAACCAGACAGC ACTGGACATAGTGAATCAGTTCACCACCTCCCAGGCCAGCCGGGAAATCAAGCAGCTACTTCGGGAGGCT TCAGGGATCTTGAAGGTTCGAGCGCTTAAGGATTTCTGGAATCTTCATGACCCCACTGCTCTCAATATCC GGGCAGGAGATGTCATCACGGTGCTTGAACAGCATCCTGATGGCCGCTGGAAGGGCCACATCCACGAGAG CCAAAGGGGCACAGACCGTGTTGGCTACTTCCCCCCGGGCATCGTCGAGGTGGTCAGCAAGCGGGTGGGC ATACCGGTGACCCGTCTCCCCTCTGCACCCACCCTGCTGCGGCCAAGCTTCTCCCGGATATCACAGCCAT CGGCTGATGATCCCCTGCCATCTCTCACCTACGGCCAGCTCCCTCGGGTGGGCCTCAGCCCAGACAGTCC AGCAGGTGACAGGAACAGCGTGGGCAGCGAGGGGAGCGTGGGCAGCATCCGCAGCGCTGGCAGTGGGCAG AGCTCTGAAGGCACCAATGGCCATAGCACTGGTCTCCTGATTGAAAATGCTCAGCCACTACCCTCTGCCA ATGAGGACCAGGTACTGCCAGGTCTGCATGCCCCGTCCCCAGCAGACAACCTGAGCCACCGTCCTCTGGC CAGTTACCGCTCTGGGGAGATCTTCACCCAGGATGTGAGACCAGAACAGCTGCTCGAAGGGAAGGACGCA CAGGCCATCCATAACTGGTTAAGTGAATTCCAGCTAGAGGGCTACACTGCCCACTTCCTGCAGGCCGGCT ATGACGTGCCAACCATCAGCCGGATGACACCTGAGGATCTGACGGCCATTGGAGTGACCAAGCCCGGACA CAGGAAGAAGATCGCCTCAGAGATTGCTCAGCTCAGCATTGCTGAATGGCTGCCCAACTATATCCCGGTG GACTTGTTGGAGTGGCTGTGTGCACTGGGGCTGCCACAGTACCACAAGCAACTGGTGAGCAGCGGCTATG ACTCCATGGGGCTGGTGGCCGATCTCACCTGGGAGGAGCTGCAGGAGATCGGCGTGAACAAGCTAGGCCA TCAGAAGAAGCTTATGCTAGGTGTGAAGAGGCTAGCAGAACTTCGGCGGGGCCTACTGCATGGCGAAGCC CTAGGTGAAGGTGGACGCCGGATGACCAGGGGTCCTGAGCTGATGGCCATTGAAGGCCTGGAGAACGGGG ACAGTCCAGCTACGGCCGGCCCTCGCCTCCTCACCTTTCAGGGCAGTGAGCTGAGCCCAGAGCTACAGGC AGCTATGGCGGGGGGTGGCCCAGAACCACTACCCCTTCCCCCTGCCCGTTCTCCTAGCCAGGAGAGCATT GGTGCACGCTCCAGGGGATCTGGTCATTCACAGGGACAGCCTGGCTCCCAGCCCAATGCTGGTGAGCCCA GTGCCCCACAGGAGAGGAACCTTCCTGAGGGTACAGAGCGCCCCTTTAAGCCTTGCTCTCCACTCCCTGG CCAAGGACCTGCCCCTTATGTCTTTATGTGTCCACAGAGCCTACCCTCTAGCCCGGCCCCAGCACCACCT CCTGGTGTTCCCCGGGCCTTCTCCTACTTGGCTGGCTCTTCTGCCGCTCCTCCAGACCCACCCCGGCCGA AACGCCGGTCTCACAGCCTGAGCCGCCCTGGCCCTGCTGAGGGGGAGGCTGAAGGGGAGGCTGAAGGGCC AGTGGGTAGTGCTTTGGGCAGCTATGCCACACTGACTCGGAGACCGGGACGCAGCACCCTTGCCCGGACT AGTCCTAGCCTGACCCCAACTCGAGGGACTCCCCGAAGCCAGTCCTTTGCCCTCCGTGCCCGTCGTAAAG GTCCCCCGCCCCCGCCCCCCAAGCGCCTCAGTTCCGTCTCTGGCTCCACTGAGCCAACTTCACTAGAGGG GACCTCAGGGCCCAAGGAAGGGGCCGCAGGTCCCCGGAGGAGAACACTGAGTGAACCAACCGGCCCCTCA GAGTCCCCTGCTCCTTCTGCGCCAACTGGCCCTGTGTCGGACACTGAGGAAGAGGAACCTGTCCTTGAGG GGACACCGCCTTCTCGGGGCAGCTCAGGGGAAGGGCTGCCATTTGCAGAGGAAGGGAACCTGACTATCAA GCAGCGACCGAAACCAGCCGGTCCCCCTCCCCGTGAGACAACTGTGCCCCCTGGCCTTGACTTCAATCTC ACAGAGTCAGACACGGTCAAACGCAGGCCCAAATGCAAGGAGAGAGAGCCACTACGGACTGCACTGTTGG CCTTTGGGGTAGTGGGCAGTGATACCCCTGGCCCCTCCACTCCCCTGTCCTCCCAGGCCCCGTGTGAAGC TCCCTCAGCTCCCTCTAACCCTCCACGGTCTGAGCCCAGCAGCCTTCCATCTCAAGAAGCCCCAGCTCCT CTCAGCCCCGTAACTCAGCCCCCTGGGCACCCAGGGCCCAGTGCTGGACCAGGTCTGGAAACCTCAACAG GCAGTAGACAGAACGTGGACACGGAGCCTCCAGCTCCCCCCGCTGCCCTCCTCAAAGTGCCTGGAGCAGG AACAGCTCCCAAGCCTGTGTCTGTGGCCTGCACTCAGCTGGCATTTTCTGGCCCAAAGCTGGCTCCCCGG CTCGGCCCTCGCCCAGTTCCCCCTCCAAGGCCTGAGAATACCGGGCCTGTGTGTCCAGGTCGGGCCCAAC AGAGACTGGAGCAGACCAGCTCATCCTTGGAAGCTGCACTGAGGGCGGCTGAGAAGAACATTGGCTCTGA GGAACAAGATGGCCCCACAGGGCCATCCACCAAGCACATTCTGGACGACATCAGTACCATGTTTGATGCC CTGGCTGACCAGTTGGATGCCATGCTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213130 representing NM_001107065 Red=Cloning site Green=Tags(s) MGREQDLILAVKNGDVTCVQKLVAKVKAAKTKLLGSTKRLNINYQDADGFSALHHAALGGSLELIALLLE AQATVDIKDSNGMRPLHYAAWQGRLEPVKLLLRASAAVNAASLDGQIPLHLAAQYGHYEVSEMLLQHQSN PCLVNKLKKTPLDLACEFGRLKVAQLLLNSHLCVALLEGEAKDPCDPNYTTPLHLAAKNGHREVIRQLLK AGIEINRQTKTGTALHEAALYGKTEVVRLLLEGGVDVNIRNTYNQTALDIVNQFTTSQASREIKQLLREA SGILKVRALKDFWNLHDPTALNIRAGDVITVLEQHPDGRWKGHIHESQRGTDRVGYFPPGIVEVVSKRVG IPVTRLPSAPTLLRPSFSRISQPSADDPLPSLTYGQLPRVGLSPDSPAGDRNSVGSEGSVGSIRSAGSGQ SSEGTNGHSTGLLIENAQPLPSANEDQVLPGLHAPSPADNLSHRPLASYRSGEIFTQDVRPEQLLEGKDA QAIHNWLSEFQLEGYTAHFLQAGYDVPTISRMTPEDLTAIGVTKPGHRKKIASEIAQLSIAEWLPNYIPV DLLEWLCALGLPQYHKQLVSSGYDSMGLVADLTWEELQEIGVNKLGHQKKLMLGVKRLAELRRGLLHGEA LGEGGRRMTRGPELMAIEGLENGDSPATAGPRLLTFQGSELSPELQAAMAGGGPEPLPLPPARSPSQESI GARSRGSGHSQGQPGSQPNAGEPSAPQERNLPEGTERPFKPCSPLPGQGPAPYVFMCPQSLPSSPAPAPP PGVPRAFSYLAGSSAAPPDPPRPKRRSHSLSRPGPAEGEAEGEAEGPVGSALGSYATLTRRPGRSTLART SPSLTPTRGTPRSQSFALRARRKGPPPPPPKRLSSVSGSTEPTSLEGTSGPKEGAAGPRRRTLSEPTGPS ESPAPSAPTGPVSDTEEEEPVLEGTPPSRGSSGEGLPFAEEGNLTIKQRPKPAGPPPRETTVPPGLDFNL TESDTVKRRPKCKEREPLRTALLAFGVVGSDTPGPSTPLSSQAPCEAPSAPSNPPRSEPSSLPSQEAPAP LSPVTQPPGHPGPSAGPGLETSTGSRQNVDTEPPAPPAALLKVPGAGTAPKPVSVACTQLAFSGPKLAPR LGPRPVPPPRPENTGPVCPGRAQQRLEQTSSSLEAALRAAEKNIGSEEQDGPTGPSTKHILDDISTMFDA LADQLDAMLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107065
ORF Size	3600 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107065.1, NP_001100535.1
RefSeq Size	4879 bp
RefSeq ORF	3603 bp
Locus ID	303678
MW	127 kDa

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