Ppl (NM_001106976) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213102 representing NM_001106976 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCGCTCTTCAGGAAGAAAAACAAAGGCAAATACAGCCCAACGGTGCAGACGCGGAGCATCTCCA ACAAGGAACTCTCAGATCTGATTGAGCAGCTGCAGAAGAATGCGGACCAGGTGGAAAGGAACATTGTGGA CACCGAGGCCAAGATGCAGAGTGACTTGGCCCGGATGCAAGACGGGCAGCTCCCTGAGCACCGGGACGCG ACCCTGCAGAATGTGTCTGACTCAGAAAAGCTGCTGTACGTACTGGAGGCAGACTCAGCCATCGCCAAGC ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGACATCCGCCAGCTGAAAGAGCGTGTGACCAACCT GCGGGGCAAACACAAGCAGATGTACAGCCTGGCAGTGAGAGAGGCTGACCCGAAGGTCAACTGGGATGCA CTGGTGGATGAGAAACTGGACAAGCTGAGCAGCCAGAGTTTTGGGACTGACCTTCCTCTGGTGGACAGCC AGGTGGAGCAACACAACATCTTCCACAATGAAGTCAAAGCCATCGGGCCCCACCTGGCCAAGGACAAGGA GCAGAACAGCGAACTCCAAGCCAAGTATCAGAAGTTGCTGGCAGCATCACAGGCTCGGCAGCAGCACCTG AGCTCGCTGCAGGATTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAAGCCAAGGGCC GCATACAGTATGACTGGAGTGACCGCAACCTCGACTACCCCAGCCGCCGGCGCCAGTACGAGAATTTCAT CAACCGGAACCTGGAGGCCAAAGAAGAGAGAATCAACAAACTGCACACTGAAGGCGACCAGCTGCTGACA GCCGAGCACCCGGGGAGGAACTCCATTGAGGCACACATGGAGGCGGTGCACGCGGAGTGGAAGGAGTACC TGAACCTGCTCATCTGTGAGGAGAGCCACCTGAAATACATGGAAGACTATCACCAATTTCACAAAGATAT GAAGGATGCTCAGGAACTGCTGCGCAAGGTGGACTCAGACTTGAACCAGAAATACAGCCCTGACTTCAAA GACCGATATCAGATAGAGCTGTTGCTTCGGGAGCTGGATGACCAGGAGAAGGCTCTGGACAAGTATGAGG ATGTGGTGCGGGGGCTGCAGAAGCGAGGGCAGCAAGTGCTGCCACTCAAGTACCGGCGTGAGACGCCACT CAAGCCCATCCCTGTGGAGGCCCTCTGTGACTTTGAGGGTGACCAGGGCCTAATCTCCCGGGGCTACAGC TACACGCTGCAGAAGAACAACGGGGAGAGCTGGGAGCTGACAGACAGCACTGGGAAGAAGCTGATGGCCC CAGCTGTCTGCTTCATCATTCCCCCCACCGACCCCGAGGCCTTGGCTCTCTCGGACAGCCTGGGCAGCCA GTACCGGAGTGTGCGGCAGAAGGCGGCCGGGAGCAAACACGCGCTCCAGCAGCGGCATGAGGTGCTAAGG ACAGAGAACCCTGGAGATGCCTCCGATCTTCAGGGGCGACAGCTGCTGGCTGGCTTGGACCAAGTGGCCA GTGACCTGGACCGGCAGGAGAAGGCTATCACGGGGATCCTACGGCCACCCCTTGAGCAGGGACGGGCAAT AGAAGATAGTGCTGAGCGGGCCAAGCACCTCAAGAACATTACCAATGAGCTGCTACAGATTGAGCCAGAG AAGATACAGCGCACAGCTGAGTGTGAGGCCTTCGTGCAGGCCCTCCCGGGCAGTGGCACCACACCCTTGC TGAAGACCCGGGTGGAAGATACCAACCAGAAATATAAGCGACTGGTACAGCTGCTAGAAGCAGCCCAGGA GAAGGTGGATGTTGCCAACCGCCTGGAGAACAGTCTGCAACGGGGCAGGGAGCTGCTGGCCTCCCACGAG AACAGGCTGATGCAGGATGACACAATACCTGAAAGTGACTACATGTTGGACAGCAAGAGGCAGGAGCTAG AGGCCATGGCTTCTGAGCTGCAGGCCCAGAAGTCCCTCCTGGGTGAGGTGGAACAGAACCTGCAGGTGGC CAAGCAGTGCTCCAGCTCACTGGCCAGCCGCTTCCAAGAGCACTGCCCTGACCTGGAGCGCCAGGAGGCT GAGGTACACAAGCTCAACCAGCGTTTCAACAACCTCAATCAGCAGGTGGAACGCAGGGCCCAGAGCCTGC AGAGTGCCAGGGATGCCTACAGTGAATACTGCCGAGGTTATGACCATGTGCTTCAGTTCTTGGTCAAGAC TCCCAGTTATGAGCCCCAGGAGACAGACAGCCTCAGCCAGATGGAGACCAAACTGAAGAACCAGAAGAAC TTGCTTGATGAGATAGCAAGCATGGAACAAGGGGCGCAGAAGGTCTACGCAGACTCCCAACAGTACCAGC AAGCAGTGAAGGACTATGAGCTTGAAGCAGAGAAGCTAAGGTCCCTGCTGGACCTGGAGAACGGACGGAA CAGCCATGTGAACAAGAGAGCCAGGCTGCAGTCTCCTGCTGCCAAAGTGAAGGAAGAGGAAGCTGCTCTT GCTGCCAAGTTCACAGAAGTTAATGCCATCAACAGACAGAGGCTACAAAATTTGGAGTTTGCACTGAATC TCTTGAGACAGCAACCAGAGGCAGGAGTGACCCATGAGACTCTGCAAGGGAGAGAGCAAGGCTCTGGCAT GGAGGAAACATGGAAGATTCAGAAGGAACTGGAGGAGGAGATGGAGCGAAGGCAACAGTTGGAAAATGAG GTCAAGAGTGCCCAGGAAGAAATCCAGACCCTGAAGGATCAGGGTCCTCAGGAATCATTGGTGAGGAAGG AGGTGCTGAAGAAGGTGCCAGACCCTGCCCTGGAGGAGAGCTTCCAGCAGCTGCAGCAGACCCTGGCTGA GGAACAGCACAAGAACCAGCTGTTGCAGGAGGAGCTGGGAGCACTGCAGCTCCGGCTGCAGGCCCTAGAA CAGGAGACCAGGGATGGGGGACAAGAGTATGTGGTCAAGGAGGTCTTGCGCATTGAGCCAGACAGAGCCC AGGAGGATGAGGTTTTACAGCTTCGGGAGGAGCTGGAGGGACTACGGCGCCAGAAAGGTGCCCGAGAGGC TGAGGTACTACTCTTACAGCAGCGCGTAGCAGCCCTAGCTGCTGAGAAGAGCAGGGTACAGGAAAAGGTC ACTGAGAGAGAGGTGGTAAAGCTACAGAATGACCCTCAGCTGGAGGCAGAATACCGGAGGCTACAGGAGG AACACCAACGGGAAGGTACACTCAGGGAGAAGCAGGAAGAGGAGCTGAGTTTCCTACAGGCCAAGCTCAG GAGACTGGAGAAAGAACGAGCCATGGCAGAAGGCAAGATCACTGTCAAAGAGGTGCTCAAAGTAGAGAAA GATACAGCTGCAGAGAGGGAAGTCAATGACCTCAGCCGCCAGTATGAGGATGAGGCTTCCAAGGCTCGCA CTAGCCAGCGGGAGAAGACAGAGCTTCTCAGAAAGATATGGGCCCTGGAAGAAGAAAATGCCAAGGTGGT GGTACAGGAGAAGGTCCGAGAGATTGTGCGGCCAGATCCCAAGGCAGAGAGTGAAGTGGCCAACCTCCGC CTGGAGCTGGTGGAGCAGGAGCGCAAATTCAGAGGTGCTGAGGAGCAGCTGAAGAGCTACCAGAGTGAGC TGGAGGCCCTACGGAACCGCGGGCCACAAGTAGAAGTCAAAGAGGTGACCAAAGAGGTCATTAAATACAC AACTGACCCAGAGACGGAGCAGGAACTCCAGCGACTCAGGGAGGAGATCATGGACAAGACCAGGTTAATA GAAAGGTGTGACTTAGAGATTTATCAGCTGAAGCAGGAGATCCAAATCCTGAAAGACACCAAGCCACAGG TGCAGACTAGAGAGGTGGTCCAGGAGATCCTGCAGTTCCAGGAAGACCCCCAAACCAAGAAGGAGGTAGA GTCTCTGCGTATACAGCTGTCAGAAGAAAAGAAGAAACTGGTGGACCTGGAAGGGGAGCAGGCATCCCAG GAAGAGAAGATCAAACGCAAGGAGGAAGAGCTGGCCCAGGGTAAGGAAAGGATTGTACGCCAGGAGGTGG TACAGTATGAAGATGAGCCAGATTTGCGGGCTGAGGTGACTGCCTTCACAGACAGCATTGATGTTGAGCT GAGGCAGATTGATAAACTGTGTGTGGAGCTACGGCGGCTACAGCATCGCAGAGCAGAGCTGGAGAGGCAG CTGGAGGAGCTGGAGCGTGAGAGGCAGGCACGTAGGGCAGCTGAGCTGGAGGTGCAGAGGCTGCAACAGC GGCTGGCTGCACTGGAGCAAGAGGAGGCCAAGACTGGTGAGAAAGTGACCCACACACAAAAGGTGGTGCT GCAGCAGGACCCACAGCAAACCAGGGAACATGCCCTGCTCCGAGCCCAGCTGGAGGAAGAGCGACACCGG AGGCAGCTACTGGAGGGTGAGCTTGAGCCCCTGCGTAGAAAGTTGGCTGCCCTGGAGAAGACAGAGATCA AAGAAAAGGTGGTCTTCTCCGAGAGTGTCCAGGTAGAAAAGGGTGACACTGAGCAAGAGATCCAGCGGCT CAAGAAGAGCCTGGAAGAGGAGAGCCGGAGCAAGAGGGAGCTAGATTCAGAGGTGACCCGGCTGGAAGCC AAGTTATCTGAGCTAGAATTCTACAATTCCAAGTCATCCAAGGAACTGGATTTCCTGAGAGAAGAAAACC ACAAGCTACAGCTAGAGCGGCAAAACCTGCAGCTAGAGACCCGGAGACTCCAATCAGAGATTGAGATGGC CGCAACAGAAACTCGAGATCTGAAGAACATAACCATGGTAGACTCTGGAACCCACCTAAACTCTAGGCTG TGGTCCCTAGAGAAGGAACTGGATGACCTCAAGAAGATGTCCAAGGACAAGGATCTGGAGATCGATGAAC TGCAGAGGCGTCTGGGCTCTGTAGCCGTCAAGAGGGAGCAGAGGGAGAACCACCTGAGGCGCTCCATAGT GGTCATTGACCCTGACACAGGTCGTGAGCTGTCCCCAGAGGAGGCCCACCGGGCTGGGCTCATCGACTGG AAAATGTTCGTGAAGCTCAGAAGCCAGGAGTGTGACTGGGAAGAGATATCAGTGAAAGGTCCTAATGGGG AGTCATCAGTAATCCATGACAGGAAGTCTGGCAAGAAGTTTTCCATTGAGGATGCTCTGCAGAGTGGCAG GCTAACCCCTGCTCAATATGACCGCTATGTCAACAAGGATATGTCCATTCAGGAGCTGGCAGTCTTGGTG TCTGGGCAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213102 representing NM_001106976 Red=Cloning site Green=Tags(s) MHSLFRKKNKGKYSPTVQTRSISNKELSDLIEQLQKNADQVERNIVDTEAKMQSDLARMQDGQLPEHRDA TLQNVSDSEKLLYVLEADSAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQMYSLAVREADPKVNWDA LVDEKLDKLSSQSFGTDLPLVDSQVEQHNIFHNEVKAIGPHLAKDKEQNSELQAKYQKLLAASQARQQHL SSLQDYMQRCTNELYWLDQQAKGRIQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHTEGDQLLT AEHPGRNSIEAHMEAVHAEWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFK DRYQIELLLRELDDQEKALDKYEDVVRGLQKRGQQVLPLKYRRETPLKPIPVEALCDFEGDQGLISRGYS YTLQKNNGESWELTDSTGKKLMAPAVCFIIPPTDPEALALSDSLGSQYRSVRQKAAGSKHALQQRHEVLR TENPGDASDLQGRQLLAGLDQVASDLDRQEKAITGILRPPLEQGRAIEDSAERAKHLKNITNELLQIEPE KIQRTAECEAFVQALPGSGTTPLLKTRVEDTNQKYKRLVQLLEAAQEKVDVANRLENSLQRGRELLASHE NRLMQDDTIPESDYMLDSKRQELEAMASELQAQKSLLGEVEQNLQVAKQCSSSLASRFQEHCPDLERQEA EVHKLNQRFNNLNQQVERRAQSLQSARDAYSEYCRGYDHVLQFLVKTPSYEPQETDSLSQMETKLKNQKN LLDEIASMEQGAQKVYADSQQYQQAVKDYELEAEKLRSLLDLENGRNSHVNKRARLQSPAAKVKEEEAAL AAKFTEVNAINRQRLQNLEFALNLLRQQPEAGVTHETLQGREQGSGMEETWKIQKELEEEMERRQQLENE VKSAQEEIQTLKDQGPQESLVRKEVLKKVPDPALEESFQQLQQTLAEEQHKNQLLQEELGALQLRLQALE QETRDGGQEYVVKEVLRIEPDRAQEDEVLQLREELEGLRRQKGAREAEVLLLQQRVAALAAEKSRVQEKV TEREVVKLQNDPQLEAEYRRLQEEHQREGTLREKQEEELSFLQAKLRRLEKERAMAEGKITVKEVLKVEK DTAAEREVNDLSRQYEDEASKARTSQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLR LELVEQERKFRGAEEQLKSYQSELEALRNRGPQVEVKEVTKEVIKYTTDPETEQELQRLREEIMDKTRLI ERCDLEIYQLKQEIQILKDTKPQVQTREVVQEILQFQEDPQTKKEVESLRIQLSEEKKKLVDLEGEQASQ EEKIKRKEEELAQGKERIVRQEVVQYEDEPDLRAEVTAFTDSIDVELRQIDKLCVELRRLQHRRAELERQ LEELERERQARRAAELEVQRLQQRLAALEQEEAKTGEKVTHTQKVVLQQDPQQTREHALLRAQLEEERHR RQLLEGELEPLRRKLAALEKTEIKEKVVFSESVQVEKGDTEQEIQRLKKSLEEESRSKRELDSEVTRLEA KLSELEFYNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEIEMAATETRDLKNITMVDSGTHLNSRL WSLEKELDDLKKMSKDKDLEIDELQRRLGSVAVKREQRENHLRRSIVVIDPDTGRELSPEEAHRAGLIDW KMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEDALQSGRLTPAQYDRYVNKDMSIQELAVLV SGQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106976
ORF Size	5262 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106976.1, NP_001100446.1
RefSeq Size	6281 bp
RefSeq ORF	5265 bp
Locus ID	302934
MW	204.1 kDa

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