Hgf (NM_017017) Rat Tagged ORF Clone
CAT#: RR213062
- TrueORF®
Hgf (Myc-DDK-tagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug)
"NM_017017" in other vectors (3)
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CNY 5,336.00
CNY 9,310.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HPTA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR213062 representing NM_017017
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTGCTGCACCTCCTCCTGCTTC CTGTCACCATCCCCTATGCAGAAGGACAGAAGAAGAGAAGAAATACTCTTCATGAATTCAAAAAGTCAGC AAAAACTACTCTTACCAAGGAAGACCCATTAGTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG TGTGCCAACAGGTGCATCAGAAACAAGGGCTTTCCATTCACTTGCAAGGCCTTTGTTTTTGATAAGTCGA GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT TGACCTCTATGAAAACAAAGACTATATTAGAAATTGCATCATTGGTAAAGGAGGCAGCTATAAGGGGACA GTATCCATCACTAAGAGTGGCATCAAGTGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTTTT TGCCTTCGAGCTATCGCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGG ACCCTGGTGTTTCACAAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTT GAATGCATGACCTGCAACGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACATGTC AGCGCTGGGATCAGCAGACACCACACCGGCACAAATTCTTGCCGGAAAGATATCCCGACAAGGGCTTTGA TGATAATTATTGCCGCAATCCCGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCT TGGGAGTATTGTGCAATTAAAATGTGCGCTCACAGTGCTGTGAATGAGACTGATGTTCCCATGGAAACAA CTGAATGTATAAAAGGCCAAGGAGAAGGTTACAGGGGAACCACCAATACCATTTGGAATGGAATTCCGTG TCAGCGTTGGGATTCGCAGTACCCCCACAAGCATGACATCACTCCCGAGAACTTCAAATGCAAGGACCTT AGAGAAAATTATTGCCGCAATCCGGATGGGGCTGAATCACCATGGTGTTTTACCACTGATCCAAACATCC GAGTTGGTTACTGCTCTCAAATTCCCAAATGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGG GAAAAACTACATGGGCAACTTATCCAAAACAAGGTCTGGACTCACATGTTCCATGTGGGACAAGAATATG GAGGATTTACACCGTCATATCTTCTGGGAGCCAGACGCTAGCAAGTTGACTAAGAATTACTGCCGGAACC CCGATGACGACGCCCATGGACCTTGGTGCTACACAGGGAATCCTCTCGTTCCTTGGGATTATTGCCCTAT TTCCCGTTGTGAAGGAGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAA ACAAAACAACTGCGAGTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAAT ACAGGAATAAACACATCTGTGGGGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGGCAATGTTT TCCAGCTAGAAACAAAGACTTGAAAGACTATGAAGCTTGGCTTGGAATCCATGATGTCCATGAGAGAGGC GAGGAGAAACGCAAACAGATCTTAAACATTTCCCAGCTAGTCTATGGACCTGAAGGCTCAGATTTGGTTT TACTGAAGCTTGCTCGCCCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGCTG TACAATCCCTGAAAAGACTACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCAGATGGT TTATTACGAGTAGCTCATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGA CTTTGAATGAGTCTGAATTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCTTGTGAGGGAGATTATGG TGGCCCACTCATTTGTGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGT GCCATCCCAAATCGTCCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTT TGACATACAAGTTGTGACATACAAGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213062 representing NM_017017
Red=Cloning site Green=Tags(s) MMWGTKLLPVLLLQHVLLHLLLLPVTIPYAEGQKKRRNTLHEFKKSAKTTLTKEDPLVKIKTKKVNSADE CANRCIRNKGFPFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGT VSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEV ECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTP WEYCAIKMCAHSAVNETDVPMETTECIKGQGEGYRGTTNTIWNGIPCQRWDSQYPHKHDITPENFKCKDL RENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNM EDLHRHIFWEPDASKLTKNYCRNPDDDAHGPWCYTGNPLVPWDYCPISRCEGDTTPTIVNLDHPVISCAK TKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERG EEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADG LLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGC AIPNRPGIFVRVAYYAKWIHKVILTYKL*HTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017017 |
ORF Size | 2184 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2532 bp |
RefSeq ORF | 2187 bp |
Locus ID | 24446 |
UniProt ID | P17945 |
MW | 82.9 kDa |
Gene Summary | plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN213062 | Hgf (untagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug) |
CNY 9,310.00 |
|
RR213062L3 | Lenti ORF clone of Hgf (Myc-DDK-tagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug) |
CNY 11,120.00 |
|
RR213062L4 | Lenti ORF clone of Hgf (mGFP-tagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug) |
CNY 12,260.00 |