Kif21b (NM_001105990) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC189181
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212904 representing NM_001105990 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGTCAGGGCGACTGCTGCGTCAAAGTGGCCGTCAGGATCCGGCCCCAGCTATCAAAGGAGAAGA TTGAAGGCTGTCACATCTGTACCTCCGTTACCCCTGGGGAGCCCCAGGTCCTGCTGGGGAAGGACAAGGC CTTCACCTATGACTTTGTCTTTGACCTGGACACCTGGCAAGAACAAATCTATTCGACCTGCGTGAGCAAA CTCATCGAAGGCTGCTTTGAGGGCTATAACGCCACAGTACTGGCCTATGGACAGACAGGGGCTGGGAAGA CATACACTATGGGCACCGGCTTTGACACAGTGACGTCAGAAGAGGAGCAGGGCATCATCCCAAGGGCCAT TGCGCATCTCTTCAGGGGCATCGACGAACGCAAGCGTCGGGCGCAGGAGCAGGGTGTGACTGGGCCTGAG TTCAAAGTCAGTGCCCAATTCCTGGAGCTCTACAACGAAGAGATCCTTGACCTGTTTGACAGCACCCGCG ACCCTGATGCCCGCCACCGCAGGTCCAACATCAAGATCCACGAGGATGCCAATGGCGGCATCTACACCAC AGGGGTCACTTCTCGCCTCATCAACTCCCAGGAGGAGCTGATCCAGTGCCTGAAGCAGGGCGCCCTGTCA CGAACCACGGCCAGCACCCAGATGAACGTTCAAAGTTCCCGATCCCACGCCATCTTCACCATTCACCTGT GCCAGATGCGCGTGTGTGCCCAGCCTGACCTGGTGAATGAGACAGTGACTGGGCTTCCGGATGGAACGGC CCCCACGGGCACTGAGTATGAGACGCTCACTGCTAAGTTCCACTTTGTGGACTTGGCGGGCTCAGAGCGG CTGAAACGGACAGGGGCCACCGGTGAACGAGCCAAAGAGGGCATCTCCATCAACTGTGGTCTGCTGGCCT TGGGCAATGTGATCAGTGCCTTGGGAGATCAGAGCAAGAAAGTCGTGCACGTGCCCTACAGGGACTCCAA GCTCACTCGGCTGCTCCAGGACTCTCTTGGAGGCAACAGCCAGACCATCATGATCGCCTGTGTGAGCCCC TCAGATCGGGACTTCATGGAGACGCTCAACACTCTCAAATATGCCAATCGGGCTCGAAACATCAAAAACA AAGTGGTAGTGAACCAGGACAAGACCAGCCAGCAGATCAGCGCGCTGCGAGCCGAGATCGCACGCCTGCA GATGGAGTTGATGGAATACAAGGCGGGCAAGCGAGTGATTGGGGAGGACGGTGCAGAGGGCTACAGCGAC CTGTTCCGGGAGAACGCCATGCTACAGAAGGAGAACGGAGCACTGAGACTCCGAGTGAAGGCCATGCAGG AAGCCATTGATGCCATCAACAACCGGGTCACACAACTCATGAGTCAGGAGGCTAACCTGCTTCTGGCCAA GGCTGGGGATGGCAACGAGGCCATTGGCGCTCTGATCCAGAACTACATCCGGGAGATTGAGGAGCTTCGC ACGAAGCTTCTGGAGAGTGAGGCTATGAATGAGTCCCTCCGAAGGAGCCTCTCCCGGGCTTCCGCTCGGA ACCCCTACTCCCTGGGAGCATCTCCAGCAGGTCCAGCCTTTGGGGGCAGCCCGGCCAGCTCCATGGAAGA TGCTTCTGAAGTGATCCGCAGGGCCAAGCAAGACCTGGAGCGGCTGAAAAAGAAAGAGGTCAGGCAGCGG AGGAAGAGCCCGGAGAAAGAAGCCTTCAAAAAGAGGGCAAAACTCCAGCAGGAAAACAGTGAGGAGACGG ATGAGAACGAGGCTGAGGAGGAGGAAGAAGAACGAGATGAGAGTGGCTGTGAGGAGGAGGAGGATGAAGA CTCGGGCAGTGAAGAGAGCCTGGTGGACTCGGACTCCGACCCTGAGGAAAAGGAAGTCAACTTCCAGGCA GACCTGGCGGACCTGACCTGTGAGATTGAGATCAAGCAGAAGCTGATCGACGAGCTGGAGAACAGCCAGC GGCGGCTACAGACGCTCAAGCACCAGTACGAGGAGAAGCTCATTCTGCTGCAGAACAAGATCCGAGACAC ACAGCTGGAGCGCGACCGCGTGCTGCAGAACCTCAGTACTATGGAGTGCTACACGGAGGAGAAGGCCAAC AAGATAAAGGCAGATTATGAGAAGAGGCTTCGGGAGATGAACCGGGACCTGCAGAAGCTACAGGCTGCTC AAAAGGAGCATGCCAGGCTGCTCAAGAACCAGTCTCGCTATGAGAGGGAGCTGAAGAAGCTGCAAACGGA GGTAGCCGAAATGAAGAAGGCCAAGGTAGCCCTCATGAAGCAAATGCGTGAGGAACAGCAGCGGCGGCGA CTGGTGGAGACCAAGAGGAACCGGGAGATTGCTCAGCTCAAGAAGGAGCAACGGAGGCAGGAGTTCCAGA TACGGGCCCTGGAATCTCAGAAGCGCCAGCAGGAAATAGTTCTGAGGAGGAAAACCCAGGAGGTTTCCGC ACTGAGGCGCTTAGCAAAGCCCATGTCTGAGAGAGTGGCCGGACGTGTAGGATTGAAGCCCCCCAATATG GATTCTGGGGCTGAGGTGTCTGCCAGCACTACCTCATCTGAGGCTGAATCCGGAGCTCGCTCTGTCTCCA GCATAGTGCGTCAGTGGAACCGAAAAATCAACCACTTCCTGGGGGACCACCCGACAGCCACTGTCAATGG CGCCCGCCCGGCCAGGAAAAAGTTCCAAAAGAAGGGAGCCAGCCAAAGCTTCAGTAAGGCCGCGAGACTC AAGTGGCAGTCCCTGGAACGGAGGATCATTGACATTGTCATGCAGAGAATGACCATCGTCAACCTGGAGG CTGATATGGAGCGTCTCATAAAGAAAAGGGAGGAGCTCTTCCTCCTACAGGAGGCACTGCGGAGGAAGCG GGAGCGCCTGCAAGCTGAGAGCCCGGAGGAGGAGAAGGGACTACAGGAACTGGCTGAGGAGATCGAAGTG TTGGCAGCCAATATTGACTACATCAATGACAGCATCACTGACTGCCAAGCCACTATCATGCAGCTGGAGG AAACCAAGGAGGAGCTGGATTCGACAGACACATCGGTGGTCATTAGTTCCTGCTCTCTGGCTGAAGCCCG CCTACTGCTAGACAACTTCCTCAAGGCATCCATTGACAAGGGACTTCAAGTAGCACAGAAGGAGGCCCAG ATCCGGCTGCTGGAAGGACGCCTCAGACAAACAGACATGACAGGCTCTTCCCAGAACCACCTGCTCCTAG ATGCTTTGCGTGAGAAGGCTGAGGCACACCCCGAGCTGCAGGCCCTCATCTACAATGTGCAACATGAGAA TGGCTATGCCAGCACAGATGAGGAAGTTTCAGAGTTCTCTGAGGGGAGCTTTTCCCAGTCATTCACCATG AAAGGCTCCACCAGCCATGATGACTTCAAGTTCAAGGGTGAGCCCAAGCTGTCCGCACAAATGAAGGCCG TGTCTGCGGAGTGCCTAGGACCCCCACTGGACAGCTCCACCAAGAACATCACCAAGTCCCTGGCCTCCCT CGTTGAGATCAAAGAGGATGGGGTGGGCTTCTCTATCCGGGACCCATACTACCGAGACAAGGTCTCACGC ACCGTCAGCCTGCCCACCCGGGGCAGCACTTTCCCTCGGCAGTCTCGAGGTGCCACGGATACATCCCCTC TGACCCGCAGGAAGTCTTATGACCGGGGACAGCCCATCAGATCAACAGACATAGGATTCACACCTCCCTC ATCACCTCCCACTCGGCCCCGCAACGACCGCAATGTCTTTTCTCGCCTCACCAGCAATCAGAGCCAGGGC TCGGCACTGGACAAGTCTGATGACAGCGACTCCTCTTTGTCGGAGGTCCTGAGAGGCATCATCACCCCCA TTGGCGGAGCAAAGGGAGCGCGGACGGCCCCGTTGCAGTGTGTCTCCATGGCCGAGGGCCACACCAAGCC CATCCTCTGCCTGGATGCCACCGATGAATTACTTTTCACTGGATCCAAAGACCGTAGCTGCAAGATGTGG AACCTGGTAACAGGGCAGGAGATTGCAGCTCTCAAGGGCCACCCCAACAACGTGGTTTCTATCAAATACT GCAGCCACTCAGGGCTGGTGTTCTCTGTGTCCAGCTCCTACGTCAAGGTGTGGGACATCCGGGACTCAGC CAAGTGCATCCGGACGCTCACGTCCTCAGGCCAGGTGATCTCAGGAGATGCTTGCATGGCCACATCCACC CGTGCCATCACCAGTTCCCAGGGAGAGCATCAGATTAACCAGATGGCCCTCAGCCCCTCGGGCACCATGT TATATGTTGCCTCCGGCAACGCTGTCCGCATCTGGGAGCTCAACAGGTTCCAGCCCATTGGCAAACTGAC CGGCCACATTGGCCCAGTGATGTGCCTGACAGTCACCCAGACTTCAAACCAGCATGACCTCGTGGTGACT GGCTCCAAGGACCACTATGTGAAGATGTTCCAGCTGGGCGACTGTGTAACGGGCACCATTGGCCCAACCC ACAACTTTGAGCCCCCTCACTATGACGGTATCGAATGCCTGGCCATCCAAGGAGATATACTGTTCAGTGG TTCCAGAGACAATGGCATCAAGAAGTGGGACCTGGATCAGCAGGAGCTCATTCAGCAAATCCCCAACGCT CACAAGGACTGGGTGTGCGCCCTGGCCTTTGTACCCGGCCGGCCCATGTTACTGAGCGCCTGCCGGGCAG GCTTCATCAAGGTCTGGAATGTGGACAACTTCACGCCCATTGGTGAGATCAAGGGCCATGACAGCCCCAT CAATGCCATCTGCACCAACAGCAAGCACATCTTCACGGCCTCCAGTGACTGCCGGGTAAAGCTGTGGAAT TACGTCCCTGGACTCACCCCCTGCCTTCCTCGACGAGTCCTGGCCATAAAGGGCCGCGCCACCACCCTGC CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212904 representing NM_001105990 Red=Cloning site Green=Tags(s) MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSK LIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPE FKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALS RTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSER LKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSP SDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSD LFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELR TKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQR RKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQA DLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKAN KIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRR LVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNM DSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARL KWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEV LAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQ IRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTM KGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSR TVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQG SALDKSDDSDSSLSEVLRGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMW NLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATST RAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVT GSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNA HKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDCRVKLWN YVPGLTPCLPRRVLAIKGRATTLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105990
ORF Size	4902 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105990.1, NP_001099460.1
RefSeq Size	5387 bp
RefSeq ORF	4905 bp
Locus ID	289397
UniProt ID	F1M5N7
MW	182.6 kDa
Gene Summary	Plus-end directed microtubule-dependent motor protein which displays processive activity. Is involved in regulation of microtubule dynamics, synapse function and neuronal morphology, including dendritic tree branching and spine formation. Plays a role in lerning and memory. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptor to cell surface.[UniProtKB/Swiss-Prot Function]

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