United States
Germany
Japan
United Kingdom
China
Lrrc8d (NM_001008338) Rat Tagged ORF Clone
CAT＃: RR212831
- TrueORF®
Lrrc8d (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member D (Lrrc8d), (10 ug)
"NM_001008338" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lrrc5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212831 representing NM_001008338
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAATGTTTACCCTTGCGGAAGTTGCTTCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAGC CATGGTGGGACGTGTTTATGGATTACCTGGCGGTTGTTATGCTGATGGTAGCCATCTTTGCAGGGACCAT GCAACTTACCAAAGATCAGGTGGTCTGTTTGCCAGTGTTGCCGTCGCCTGCAAATTCAAAGGCGCACACA CCACCCGGGAATGCTGACGTCACCACTGAAGTCCCCAAGATGGAGACAGCCACACCCCAAGACCAAAACG GGCAGACGACGAATGACATTGCCTTTGGCACATCTGCTGTGACGCCTGACATACCTCTCCAAGCCACCTA TTCACACGCAGAGTCCACCTTTCCCAGTCAGGAGACGAAGAAGGAGAAGAGAGACCCCACGGGCCGCAAA ACCAACTTGGATTTTCAGCAGTACGTATTTATCAATCAGATGTGCTACCATCTGGCCCTTCCTTGGTATT CCAAGTACTTTCCATACCTTGCCCTTATACACACCATCATCCTTATGGTCAGTAGCAACTTTTGGTTCAA ATATCCCAAAACGTGCTCCAAGGTTGAGCATTTTGTTTCAATATTAGGAAAGTGTTTCGAATCTCCCTGG ACTACTAAAGCGCTGTCCGAGACAGCCTGTGAGGACTCTGAGGAGAACAAGCAGCGGATAACGGGCGCCC AGACCCTACCGAAGCACGTGTCCACCAGCAGCGATGAGGGGAGCCCCAGCGCCAGCACCCCCATGATCAA CAAAACGGGCTTCAAGTTCTCAGCTGAAAAGCCGGTGATCGAAGTTCCCAGCATGACGATCCTGGACAAG AAGGACGGGGAGCAGGCCAAAGCCCTGTTTGAGAAGGTGAGGAAATTTCGTGCCCATGTGGAAGACAGTG ACTTGATCTACAAGCTCTACGTGGTCCAGACCCTCATCAAGACCGCCAAGTTCATTTTTATCCTCTGCTA CACTGCAAACTTTGTCAACGCCATCAGCTTCGAGCATGTCTGCAAGCCAAAAGTCGAGCACCTCACGGGG TACGAGGTGTTCGAGTGCACTCACAATATGGCCTACATGTTGAAGAAACTGCTCATCAGCTACATCTCCA TCATCTGTGTCTATGGCTTCATCTGCCTCTACACTCTCTTCTGGCTGTTCAGGATCCCTCTGAAGGAATA TTCCTTTGAGAAAGTCCGAGAGGAAAGCAGCTTCAGCGACATCCCGGATGTCAAGAACGACTTCGCGTTC CTCCTGCACATGGTCGACCAGTACGACCAGCTCTATTCCAAGCGTTTTGGTGTCTTCTTATCTGAAGTCA GTGAAAACAAACTGAGGGAAATCAGCCTGAACCATGAGTGGACTTTCGAGAAACTCAGGCAGCACGTGTC TCGCAACGCCCAGGACAAGCAGGAGCTGCACTTGTTCATGCTGTCAGGGGTGCCCGACGCTGTCTTTGAC CTCACGGACCTGGATGTGCTAAAACTTGAACTGATTCCCGAAGCAAAAATTCCTGCCAAGATCTCTCAGA TGACGAACCTTCAAGAACTCCACCTCTGCCACTGCCCTGCCAAGGTGGAGCAGACCGCCTTTAGCTTTCT TCGCGATCACTTGAGATGCCTTCATGTGAAGTTCACTGACGTGGCTGAAATCCCTGCCTGGGTGTATTTG CTCAAAAACCTGCGGGAGCTGTACTTGATAGGCAACTTGAACTCAGAGAACAATAAGATGATCGGGCTGG AGTCTCTGCGAGAGCTGCGGCACCTTAAGATCCTCCACGTGAAGAGTAACCTGACCAAAGTTCCCTCCAA CATAACTGACGTGGCTCCTCACCTCACCAAGCTCGTCATCCACAATGACGGCACCAAGCTCTTGGTGCTC AACAGCCTGAAGAAGATGATGAACGTTGCCGAGCTAGAGCTGCAGAACTGTGAGCTGGAGAGGATTCCAC ACGCCATTTTCAGCCTTTCTAACTTGCAGGAGCTGGACCTGAAGTCCAACAGCATCCGCACGATCGAGGA GATCATCAGTTTCCAACACCTGAAGCGACTGACTTGCTTGAAATTGTGGCACAATAAAATTGTGGCCATT CCTCCCTCCATTACCCACGTCAAGAACCTGGAGTCCCTCTACTTCTCCAACAACAAGCTCGAGTCCTTAC CGGTGGCAGTGTTTAGTTTACAGAAACTCAGATGCTTAGATGTCAGCTATAACAACATTTCCACGATCCC CATAGAGATAGGTTTGCTTCAGAACCTGCAGCATTTGCACATCACAGGGAACAAAGTGGACGTTCTGCCA AAACAGTTGTTTAAGTGCGTGAAGTTGAGGACTTTGAACCTGGGGCAGAACTGCATCGCCTCCCTGCCTG AGAAGATCAGTCAGCTGTCCCAGCTCACTCAGCTGGAGCTGAAGGGCAACTGTCTCGACCGCCTGCCAGC CCAGCTGGGCCAGTGTCGGATGCTCAAGAAAAGCGGGCTTGTTGTAGAAGACCAACTGTTTGACACGCTG CCACTGGAAGTCAAAGAGGCATTGAATCAAGACGTAAATGTCCCCTTTGCGAACGGGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212831 representing NM_001008338
Red=Cloning site Green=Tags(s) MGMFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHT PPGNADVTTEVPKMETATPQDQNGQTTNDIAFGTSAVTPDIPLQATYSHAESTFPSQETKKEKRDPTGRK TNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPW TTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDK KDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTG YEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAF LLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFD LTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYL LKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVL NSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNSIRTIEEIISFQHLKRLTCLKLWHNKIVAI PPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDVLP KQLFKCVKLRTLNLGQNCIASLPEKISQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTL PLEVKEALNQDVNVPFANGI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008338 |
| ORF Size | 2580 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001008338.1, NP_001008339.2 |
| RefSeq Size | 3213 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 305131 |
| UniProt ID | Q5U308 |
| MW | 98.1 kDa |
| Gene Summary | Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:28833202). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress (By similarity). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:28833202).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212831 | Lrrc8d (untagged ORF) - Rat leucine rich repeat containing 8 family, member D (Lrrc8d), (10 ug) |
CNY 10,930.00 |
|
| RR212831L3 | Lenti ORF clone of Lrrc8d (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member D (Lrrc8d), (10 ug) |
CNY 12,830.00 |
|
| RR212831L4 | Lenti ORF clone of Lrrc8d (mGFP-tagged ORF) - Rat leucine rich repeat containing 8 family, member D (Lrrc8d), (10 ug) |
CNY 13,970.00 |
