Met (NM_031517) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Hgfr
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212802 representing NM_031517 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCTCCCACCGCGCTGGCACCTGGCATTCTGCTGCTGCTGCTGACCTTGGCGCAGAGGAGCCATG GGGAGTGCAAGGAGGCCCTAGTGAAGTCTGAGATGAACGTGAACATGAAGTACCAGCTTCCCAACTTCAC CGCAGAAACCCCCATCCAGAATGTCGTCCTCCATGGGCACCATATTTATCTCGGAGCCACAAACTACATT TATGTTTTAAATGACAAAGACCTTCAGAAGGTATCTGAGTTCAAGACCGGGCCCGTGGTGGAACACCCAG ATTGTTTTCCTTGTCAGGACTGCAGCAGCAAAGCCAATGTGTCAGGAGGTGTTTGGAAAGACAACGTCAA CATGGCGCTGCTTGTTGACACTTACTATGACGACCAGCTCATCAGCTGTGGCAGCGTCAACAGAGGGACC TGCCAAAGGCATGTCCTTCCTCCTGACAATGCTGCCGACATTCAGTCCGAGGTTCACTGCATGTTCTCCC CACTTGCGGAGGAAGAGTCAGGCCAGTGTCCCGACTGTGTAGTGAGTGCCCTGGGAGCCAAAGTCCTCCT GTCTGAAAAGGACCGGTTCATCAATTTCTTCGTGGGGAATACGATAAACTCTTCCTACCCTCCCGATTAT TCATTGCATTCAATATCGGTGAGGCGGCTGAAGGAAACCCAGGACGGTTTTAAGTTTTTGACAGACCAGT CCTACATTGATGTCCTGGGAGAATTCCGAGATTCCTACCCCATCAAGTACATACATGCCTTCGAAAGCAA CCATTTTATCTACTTTCTGACTGTCCAGAAGGAAACCCTAGATGCTCAGACTTTCCATACAAGAATAATC AGGTTCTGTTCTGTAGACTCTGGGTTGCACTCCTACATGGAAATGCCTCTGGAGTGCATTCTGACGGAAA AAAGAAGAAAGAGATCCACAAGGGAAGAAGTGTTTAATATCCTCCAAGCCGCGTATGTCAGTAAACCAGG GGCCAATCTTGCTAAGCAAATAGGGGCCAGCCCGTATGATGACATTCTCTACGGGGTGTTTGCACAAAGC AAGCCAGATTCTGCTGAGCCCATGAACCGATCAGCGGTCTGTGCATTCCCCATCAAATATGTCAATGACT TCTTCAACAAGATTGTCAACAAAAACAACGTACGGTGTCTCCAGCATTTTTATGGACCCAACCACGAGCA CTGTTTCAATAGGACCCTGCTGAGAAATTCATCGGGCTGCGAAGTGCGCAGTGACGAGTACCGGACGGAG TTTACCACAGCGCTGCAGGCTGTGGATTTATTCATGGGCCGGCTCAACCATGTACTCTTGACGTCTATCT CTACCTTCATCAAAGGTGACCTCACCATTGCTAATCTAGGGACATCAGAAGGTCGCTTCATGCAGGTGGT GCTCTCTCGCACAGCACATTTCACCCCCCATGTGAATTTCCTCCTGGATTCCCATCCTGTGTCTCCGGAA GTTATTGTCGAACATCCATCAAATCAAAATGGCTATACCCTGGTGGTCACAGGGAAGAAGATCACCAAGA TTCCACTGAATGGCCTAGGCTGTGGGCATTTCCAGTCCTGCAGTCAGTGTCTCTCTGCCCCCTACTTTAT ACAGTGTGGCTGGTGCCACAATCGGTGTGTGCATTCCAATGAATGCCCCAGCGGTACATGGACTCAAGAG ATCTGTCTGCCAGCAGTTTATAAGGTTTTCCCCACTAGTGCACCCCTCGAAGGAGGAACAATGCTGACCA TATGTGGCTGGGACTTTGGATTCAAGAAGAATAATAAATTTGATTTAAGGAAAACCAAAGTTCTGCTTGG CAACGAGAGCTGTACCTTGACCTTAAGCGAGAGCACGACAAATACGTTGAAATGCACAGTTGGCCCCGCG ATGAGTGAGCACTTCAATGTGTCTGTGATCGTCTCAAACAGTCGAGAGACAACACAGTACAGTGCGTTTT CCTATGTGGATCCTGTAATAACAAGTATTTCTCCAAGGTATGGTCCTCATGCCGGAGGCACCTTACTCAC TTTGACTGGAAAATACCTCAACAGCGGCAATTCTAGACACATTTCAATCGGAGGGAAAACATGTACTTTA AAAAGTGTATCAGATAGCATTCTCGAATGCTACACCCCAGGCCACACCGTCTCTGCCGAGTTTCCCGTGA AATTGAAAATCGACCTGGCTGACCGAGTGACAAGCAGCTTCAGTTACGGGGAAGACCCGTTTGTCTCTGA AATCCACCCGACCAAATCTTTTATCAGTGGTGGAAGCACAATAACGGGGATTGGAAAGAACCTGAATTCA GTTAGCACCCCAAAGCTGGTAATAGAAGTGCATGACGTGGGCGTGAACTACACCGTGGCGTGCCAACATC GCTCGAGTTCAGAGATCATCTGCTGCACCACTCCTTCCCTGCAACAGCTGGACCTGCAACTCCCCCTGAA GACCAAAGCCTTCTTCCTGCTGGACGGGATCCTTTCCAAACACTTTGATCTCACTTATGTACATGATCCT ATGTTTAAGCCTTTTGAAAAGCCAGTAATGATCTCCATGGGCAATGAGAATGTAGTGGAAATTAAGGGAG ACGATATTGACCCTGAAGCAGTTAAAGGTGAAGTGTTAAAAGTCGGGAATAAGAGCTGTGAGAATCTCCA CTGGCATTCTGAAGCTTTGTTGTGTACGGTCCCCAGTGACCTGCTGAAGCTGAACGGCGGCGAGCTAAAT ATAGAGTGGAAGCAAGCAGTCTCTTCAACTGTCCTTGGAAAAGTGATCGTTCAACCGGATCAGAATTTTG CAGGATTGATCATTGGTGCGGTCTCAATATCAGTGGTAGTTTTGTTAGTATCCGGGCTCTTCCTGTGGCT GAGAAAGAGAAAGCATAAAGATCTGGGCAGTGAATTAGTTCGCTATGACGCAAGAGTACACACTCCTCAT TTGGATAGGCTTGTAAGTGCCCGAAGTGTAAGCCCAACTACAGAGATGGTCTCAAATGAGTCTGTAGACT ACAGAGCTACTTTTCCAGAAGACCAGTTTCCCAACTCCTCTCAGAATGGAGCCTGCAGACAAGTGCAGTA TCCACTGACAGATCTGTCCCCCATCCTGACGAGTGGAGACTCTGATATATCCAGCCCATTACTACAAAAC ACTGTTCACATTGACCTCAGCGCTCTAAATCCAGAGCTGGTCCAAGCGGTGCAGCACGTAGTGATTGGAC CCAGTAGCCTGATTGTGCATTTCAATGAAGTCATAGGAAGAGGGCATTTTGGCTGTGTCTATCATGGGAC TTTGTTGGACAGTGACGGAAAGAAAATTCACTGTGCTGTGAAATCCTTGAATAGAATCACAGATATAGAA GAAGTCTCCCAGTTTCTGACTGAGGGAATCATCATGAAAGATTTCAGCCACCCCAATGTTCTCTCACTCT TGGGAATCTGCCTGCGGAGTGAAGGGTCCCCTCTGGTGGTTCTGCCCTATATGAAGCACGGAGATCTTCG CAATTTCATTCGAAACGAGACTCATAACCCAACTGTGAAAGATCTTATAGGATTCGGTCTTCAAGTAGCC AAGGGCATGAAATATCTTGCCAGCAAAAAGTTTGTCCACAGAGACTTAGCTGCAAGAAACTGCATGTTGG ATGAAAAATTCACTGTCAAGGTTGCTGATTTCGGTCTTGCCAGAGACATGTACGACAAAGAGTATTATAG CGTCCACAACAAAACGGGTGCGAAACTACCGGTGAAGTGGATGGCTTTAGAGAGTCTGCAGACGCAAAAG TTCACCACCAAGTCAGACGTGTGGTCCTTCGGTGTGCTTCTCTGGGAGCTCATGACGAGAGGAGCCCCTC CTTATCCTGACGTGAACACATTTGATATCACTATATACCTGTTGCAAGGCAGAAGACTCTTGCAACCAGA GTACTGTCCAGACGCCTTGTATGAAGTGATGCTAAAATGCTGGCACCCCAAAGCAGAAATGCGCCCATCG TTTTCTGAACTGGTCTCCAGAATATCCTCAATCTTCTCCACTTTCATTGGCGAGCACTATGTCCATGTGA ACGCTACTTATGTGAATGTAAAATGTGTTGCTCCATATCCTTCTCTGTTGCCATCCCAAGACAACATTGA CGGCGAAGCGAACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212802 representing NM_031517 Red=Cloning site Green=Tags(s) MKAPTALAPGILLLLLTLAQRSHGECKEALVKSEMNVNMKYQLPNFTAETPIQNVVLHGHHIYLGATNYI YVLNDKDLQKVSEFKTGPVVEHPDCFPCQDCSSKANVSGGVWKDNVNMALLVDTYYDDQLISCGSVNRGT CQRHVLPPDNAADIQSEVHCMFSPLAEEESGQCPDCVVSALGAKVLLSEKDRFINFFVGNTINSSYPPDY SLHSISVRRLKETQDGFKFLTDQSYIDVLGEFRDSYPIKYIHAFESNHFIYFLTVQKETLDAQTFHTRII RFCSVDSGLHSYMEMPLECILTEKRRKRSTREEVFNILQAAYVSKPGANLAKQIGASPYDDILYGVFAQS KPDSAEPMNRSAVCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEVRSDEYRTE FTTALQAVDLFMGRLNHVLLTSISTFIKGDLTIANLGTSEGRFMQVVLSRTAHFTPHVNFLLDSHPVSPE VIVEHPSNQNGYTLVVTGKKITKIPLNGLGCGHFQSCSQCLSAPYFIQCGWCHNRCVHSNECPSGTWTQE ICLPAVYKVFPTSAPLEGGTMLTICGWDFGFKKNNKFDLRKTKVLLGNESCTLTLSESTTNTLKCTVGPA MSEHFNVSVIVSNSRETTQYSAFSYVDPVITSISPRYGPHAGGTLLTLTGKYLNSGNSRHISIGGKTCTL KSVSDSILECYTPGHTVSAEFPVKLKIDLADRVTSSFSYGEDPFVSEIHPTKSFISGGSTITGIGKNLNS VSTPKLVIEVHDVGVNYTVACQHRSSSEIICCTTPSLQQLDLQLPLKTKAFFLLDGILSKHFDLTYVHDP MFKPFEKPVMISMGNENVVEIKGDDIDPEAVKGEVLKVGNKSCENLHWHSEALLCTVPSDLLKLNGGELN IEWKQAVSSTVLGKVIVQPDQNFAGLIIGAVSISVVVLLVSGLFLWLRKRKHKDLGSELVRYDARVHTPH LDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGACRQVQYPLTDLSPILTSGDSDISSPLLQN TVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDSDGKKIHCAVKSLNRITDIE EVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVA KGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQK FTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITIYLLQGRRLLQPEYCPDALYEVMLKCWHPKAEMRPS FSELVSRISSIFSTFIGEHYVHVNATYVNVKCVAPYPSLLPSQDNIDGEANT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031517
ORF Size	4146 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031517.2, NP_113705.1
RefSeq Size	4187 bp
RefSeq ORF	4149 bp
Locus ID	24553
UniProt ID	P97523
MW	153.8 kDa
Gene Summary	hepatocyte growth factor receptor; functions as a heterodimeric tyrosine kinase [RGD, Feb 2006]

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