Akap4 (NM_024402) Rat Tagged ORF Clone
CAT#: RR212564
- TrueORF®
Akap4 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 4 (Akap4), (10 ug)
"NM_024402" in other vectors (3)
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CNY 10,830.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212564 representing NM_024402
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTGCTTACTGTGGCACTACAAAGATGTCTGATGACATTGACTGGTTACACAGCCGCAGAGGCGTGT GCAAGGTAGATCTTTACAGCCCAGAAGGACAACAAGATCAGGACCGAAAAGTGATATGCTTTGTGGATGT GTCCACCTTGAATGTGGAAGATGATTCCAAGGGTGCTGCTGGTCCCAGGTCAGACGGCGAATTAAACCTG GAGAATCTGGAAGAAAAAGAGATTATCGTGATCAAGGACACAGAAAAACAAGACCAGCCTAAGACGGAGG GATCTGTATGCCTTTTCAAACAAGCTCCCTCTGACCCCATAAGTGTCCTCAACTGGCTCCTCAATGATCT CCAGAAGTACGCCTTGGGTTTCCAACATGCATTAAGTCCCTCAGCCTCTAGCTGTAAACATAAAGTAGGA GACCTAGAGGGTGATTATCACAAAATTCCCTCAGAGAACTGCTACAGTGTGTATGCTGATCAAGTAAACT TAGATTATTTGAACAAAGGACCTCAAAACCTTCGCCTAGAAATGGCGGCATCCAAAAATACCAACAATAA CCAAAGTCCCTCAAATCCTGCAACTAAATCTCCTAGCAATCAGAGGTCAGTTGCCACTCCTGATGGAGAA TGTTCGATGGACGACCTCTCCTACTATGTCAACCGATTGTCATCTCTAGTAATCCAAATGGCCCGTAAGG AAATCAAGGACAAATTGGAAGGTGGAAACAAATGCCTCCATCATTCCATGTATACATCAGGAGAAAAGGG GAAAACCAGCCCCCGGAGTGCTGTCAGCAAAATTGCTTCTGAGATGGCCCATGAAGCTGTTGAACTGACC TCATCAGAAATGCGTGGCAATGGAGAAGAAGGCAGGGATGGCCGGAAAACCTTTTTGTATAGTGAGTTAT CTAATAAGAACAAGTGTGGAGAGAAGCAGCAGATGTGCCCAAAAGACAGCAAAGAATTTGCAGATTCCAT CAGCAAGGGGCTTATGGTTTATGCAAATCAGGTAGCATCTGACATGATGGTCTCTGTTATGAAAACCTTG AAAGTGCACAGCTGTGGGAAGCCAATTCCAGCTTGTGTGGTCCTGAAGAGGGTACTATTAAAGCACACCA AGGAAATTGTATCTGATCTGATTGACTCATGCATGAAGAACTTGCATAATATAACAGGAGTCCTGATGAC AGACTCAGACTTCGTTTCTGCTGTCAAGAGGAATCTTTTCAATCATGGTAAACAAAATGCAGCAGACATC ATGGAGGCCATGCTAAAGCGTCTGGTCAGTGCTCTTCTTGGTGAGAAGAAGGAGACTAAATCTCAAAGTT TGGCATATGCAACCTTAAAAGCTGGAACTCATGACCCGAAATGCAAGAATCAGAGCCTTGAGTTCTCAGC TATGAAAGCTGAAATGAAAGGAAAGGACAAAGGCAAAACTAAAGGAGATCCATGCTGTAAATCACTGACA AGTGCTGAGAGAGTCAGCGAACATATCCTCAAAGAGAGCCTTACTATGTGGAACAACCAGAAGCAAGGAA CCCAAGGCAGGGTGCCTAACAAAGTATGCCCTAGTAAAGATGAAAAGAGAGAGAAGATCAGTCCTTCCAC AGACTCACTGGCCAAGGATCTAATTGTCTCTGCCCTTATGCTCATTCAATACCATCTGACCCAACAAGCG AAGGGCAAAGATCCATGTGAAGAGGAGTGCCCTGGTTCCTCCATGGGCTATATGTCGCAAAGTGCACAAT ATGAAAAGAGTGGAGGTGGCCAAAGTTCTAAATCACTTTCAATGAAGCATTTTGAATCTCGTGGAGCTCC TGGACCATCTACATGTGCGAAGGAAAATCAACTTGAGTCCCAGAAGATGGATATGTCAAACATGGTTCTG TCCCTGATTCAGAAACTTCTGAGTGAGAGCCCTTTCAGCTGTGATGAACTATCTGAAAGTGAGAATAAGC GTTGTTGTGATTCTCGATCAAAGCAAGCAGCTCCCGTGGCCAAGAGACCTGAAGATCAAAGCCAAGACAG CACAGAAATGGACTTCATCAGCGGGATGAAGCAAATGAACCGGCAGTTTATAGACCAACTAGTAGAATCG GTGATGAAACTGTGCCTGATCATGGCTAAGTACAGCAACAATGGAGCAGCACTAGCTGAGCTAGAAGAGC AAGCAGCCCTAGCAAGCAATGGCCCCAGATGTGGCCGTGAGGCTGTGATGTCACAAAGTTATCTGGAAAC TCCTGGCCCTGAAGTTATCGTCAACAATCAGTGCTCTACAAGCAACTTGCAGAAGCAGCTGCAGGCTGTC CTGCAATGGATTGCAGCGTCTCAGTTTAATGTGCCCATGCTCTACTTCATGGGAGATGATGATGGACAAC TAGAGAAGCTCCCTGAAGTTTCAGCTAAGGCAGCAGAGAAAGGTTACAGTGTAGGAGATCTTCTTCAGGA GGTCATGAAGTTTGCCAAAGAACGACAACTGGATGAAGCCGTGGGCAACATGGCTAGAAAGCAACTGCTG GACTGGCTTCTCGCTAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212564 representing NM_024402
Red=Cloning site Green=Tags(s) MIAYCGTTKMSDDIDWLHSRRGVCKVDLYSPEGQQDQDRKVICFVDVSTLNVEDDSKGAAGPRSDGELNL ENLEEKEIIVIKDTEKQDQPKTEGSVCLFKQAPSDPISVLNWLLNDLQKYALGFQHALSPSASSCKHKVG DLEGDYHKIPSENCYSVYADQVNLDYLNKGPQNLRLEMAASKNTNNNQSPSNPATKSPSNQRSVATPDGE CSMDDLSYYVNRLSSLVIQMARKEIKDKLEGGNKCLHHSMYTSGEKGKTSPRSAVSKIASEMAHEAVELT SSEMRGNGEEGRDGRKTFLYSELSNKNKCGEKQQMCPKDSKEFADSISKGLMVYANQVASDMMVSVMKTL KVHSCGKPIPACVVLKRVLLKHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNHGKQNAADI MEAMLKRLVSALLGEKKETKSQSLAYATLKAGTHDPKCKNQSLEFSAMKAEMKGKDKGKTKGDPCCKSLT SAERVSEHILKESLTMWNNQKQGTQGRVPNKVCPSKDEKREKISPSTDSLAKDLIVSALMLIQYHLTQQA KGKDPCEEECPGSSMGYMSQSAQYEKSGGGQSSKSLSMKHFESRGAPGPSTCAKENQLESQKMDMSNMVL SLIQKLLSESPFSCDELSESENKRCCDSRSKQAAPVAKRPEDQSQDSTEMDFISGMKQMNRQFIDQLVES VMKLCLIMAKYSNNGAALAELEEQAALASNGPRCGREAVMSQSYLETPGPEVIVNNQCSTSNLQKQLQAV LQWIAASQFNVPMLYFMGDDDGQLEKLPEVSAKAAEKGYSVGDLLQEVMKFAKERQLDEAVGNMARKQLL DWLLANL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024402 |
ORF Size | 2541 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024402.1, NP_077378.1 |
RefSeq Size | 2786 bp |
RefSeq ORF | 2544 bp |
Locus ID | 79254 |
UniProt ID | O35774 |
MW | 93.5 kDa |
Gene Summary | Major structural component of sperm fibrous sheath. May play a role in sperm motility (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212564 | Akap4 (untagged ORF) - Rat A kinase (PRKA) anchor protein 4 (Akap4), (10 ug) |
CNY 10,830.00 |
|
RR212564L3 | Lenti ORF clone of Akap4 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 4 (Akap4), (10 ug) |
CNY 12,640.00 |
|
RR212564L4 | Lenti ORF clone of Akap4 (mGFP-tagged ORF) - Rat A kinase (PRKA) anchor protein 4 (Akap4), (10 ug) |
CNY 13,870.00 |