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Dram2 (NM_001025018) Rat Tagged ORF Clone
CAT＃: RR212454
- TrueORF®
Dram2 (Myc-DDK-tagged ORF) - Rat transmembrane protein 77 (Tmem77), (10 ug)
"NM_001025018" in other vectors (3)
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CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Tmem77 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212454 representing NM_001025018
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTGGTTTCAGCAAGGCCTTAGCTTCCTTCCGTCGGCCCTTGTAATTTGGACATTTGCTACCTTTA TATTCTCATATATCACTGCAATAACACTCCACCATGTTGACCCTGCATTGCCTTACATCAGCGACACTGG GACAATGCCTCCTGAAAGGTGCCTCTTTGGAGTAATGCTGAATATCGCTGCCGTTTTAGGCATTGCTACT ATCTATGTTCGTTACAAGCAAGTTCATGCACTGAACCCTGAAGAGAACCTTATCATCAAATTAAACAAGG CTGGCCTTGTACTTGGGATACTGAGTTGTTTAGGACTTTCTCTTGTGGCAAATTTCCAGAAATCAGCACT ATTTATTGTTCATGTATGTGGAGCTGTCCTTGCCTTTAGTATGGGCTCATTTTACATGTTTGTTCAGACC ATCCTTTCCTACCAAATGCAGCCCAAAATTCACAGCAAACAAGTCTTCTGGGTCCGACTACTATTGGTTA TCTGGTGTGGAGTAAGTGCACTTAGCATGATGACTTGCTCATCAATTTTGTACAGTAGTGACTTTGGTGC TGATATAGTACAGAAACTACACTGGAACCCAGAGGACAAAGGTTATGTGCTTCACATAGTTACTACTGCA GCAGAATGGTCTATGTCATTTTCCTTCTTTGGATTTTTCCTGACTTATATTCGTGATTTTCAGAAAATTA CTTTACGGGTGGAAGCCAATTTACATGGATTAACCCTCTATGACACTGTTCCTTGCCCTGTTACCAATGA AAGAACACCATTGCTTTCCAGAGATTTTCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212454 representing NM_001025018
Red=Cloning site Green=Tags(s) MWWFQQGLSFLPSALVIWTFATFIFSYITAITLHHVDPALPYISDTGTMPPERCLFGVMLNIAAVLGIAT IYVRYKQVHALNPEENLIIKLNKAGLVLGILSCLGLSLVANFQKSALFIVHVCGAVLAFSMGSFYMFVQT ILSYQMQPKIHSKQVFWVRLLLVIWCGVSALSMMTCSSILYSSDFGADIVQKLHWNPEDKGYVLHIVTTA AEWSMSFSFFGFFLTYIRDFQKITLRVEANLHGLTLYDTVPCPVTNERTPLLSRDFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025018 |
| ORF Size | 801 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025018.1, NP_001020189.1 |
| RefSeq Size | 1435 bp |
| RefSeq ORF | 804 bp |
| Locus ID | 362011 |
| UniProt ID | Q5BK09 |
| MW | 30.2 kDa |
| Gene Summary | Plays a role in the initiation of autophagy. In the retina, might be involved in the process of photoreceptor cells renewal and recycling to preserve visual function. Induces apoptotic cell death when coexpressed with DRAM1.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53
,null,
Journal of Experimental & Clinical Cancer Research : CR
,PubMed ID 30755245
[Dram2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212454 | Dram2 (untagged ORF) - Rat transmembrane protein 77 (Tmem77), (10 ug) |
CNY 3,990.00 |
|
| RR212454L3 | Lenti ORF clone of Dram2 (Myc-DDK-tagged ORF) - Rat transmembrane protein 77 (Tmem77), (10 ug) |
CNY 6,080.00 |
|
| RR212454L4 | Lenti ORF clone of Dram2 (mGFP-tagged ORF) - Rat transmembrane protein 77 (Tmem77), (10 ug) |
CNY 6,650.00 |
