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Mecom (NM_001106423) Rat Tagged ORF Clone
CAT＃: RR212298
- TrueORF®
Mecom (Myc-DDK-tagged ORF) - Rat ecotropic viral integration site 1 (Evi1), (10 ug)
"NM_001106423" in other vectors (3)
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CNY 11,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Evi1; Mds1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212298 representing NM_001106423
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGTGGAAAGCACTATGAGTGTGAAAACTGTGCCAAGCAGGTTTTCACGGACCCTAGCAACCTTCA GCGACACATTCGATCTCAGCATGTTGGTGCCCGGGCTCATGCTTGCCCGGAGTGTGGTAAAACATTTGCC ACTTCGTCAGGCCTCAAACAACACAAGCAGTGAGGTCTGCCATAAATCCTATACTCAGTTTTCAAACCTT TGTCGTCATAAGCGCATGCATGCTGATTGCAGAACCCAAATCAAGTGCAAAGACTGTGGACAAATGTTCA GCACTACGTCTTCCTTAAATAAACACAGGAGGTTTTGTGAGGGCAAGAACCATTTTGCGGCAGGTGGATT TTTTGGCCAAGGCATTTCACTTCCTGGAACCCCAGCTATGGATAAAACGTCCATGGTTAATATGGGCCAC GCCAACCCGGGCCTGGTTGACTATTTTGGCACCAATAGGCATCCTGCTGGTCTTACCTTTCCAACAGCTC CGGGATTTTCCTTGAGCTTTCCTGGTCTGTTTCCTTCGGGCTTGTACCACAGGCCTCCTTTGATACCCGC TAGCTCTCCTGTTAAAGGACTATCAAGTACCGAACAGTCAAACAAATGTCAAAGTCCCCTCTTGACACAT CCTCAGATACTGCCAGCCACCCAGGATATTTTGAAGGCACTATCTAAACACCCACCTGTAGGGGACAATA AGCCAGTGGAACTGCTGCCCGAGAGGTCCTCTGAAGAGAGGCCCCTTGAGAAAATCAGTGACCAGTCAGA GAGTAGTGACCTTGATGATGTCAGTACGCCAAGTGGCAGTGATCTGGAAACAACCTCGGGCTCTGATCTG GAAAGTGACCTTGAAAGTGATAAAGAGAAATGTAAAGAAAATGGTAAAATGTTCAAAGACAAAGTAAGCC CTCTGCAGAATCTGGCTTCAATAAATAATAAGAAAGAATACAGCAATCATTCCGTTTTCTCAGCATCTGT AGAGGAGCAGACCGCGGTGTCAGGAGCTGTGAATGATTCTATAAAGGCTATTGCGTCTATTGCTGAAAAA TACTTTGGTTCCACAGGACTGGTGGGGCTGCAAGACAAAAAAGTCGGAGCGTTACCTTACCCTTCCATGT TTCCCCTCCCGTTTTTTCCAGCATTCTCTCAATCAATGTACCCATTTCCTGATAGAGACTTGAGGTCGTT ACCTTTGAAAATGGAGCCCCAATCACCAAGTGAAGTAAAGAAACTGCAGAAGGGAAGCTCTGAGTCCCCT TTTGACCTCACCACTAAGAGAAAGGACGAGAAGCCCTTGACTTCAGGCTCCTCAAAGCTTCCAGCAACAC CCGCCACAAGCCAAGACCAGCCCCTGGATCTAAGTATGGGCAGTAGGAGTAGAGCCAGTGGGACAAAGTT GACTGAGCCTCGAAAAAACCATGTGTTTGGGGAAAAGAAAGGAAGCAACATGGAAACAAGGCCATCTTCA GATGGCTCCTTGCAGCATGCCAGACCCACTCCCTTCTTCATGGATCCCATTTATAGAGTAGAGAAAAGAA AGCTCACTGATCCGCTTGAAGCTTTGAAAGAAAAATACTTGAGACCTTCTCCAGGATTCTTGTTTCACCC ACAATTCCCACTACCTGACCAGAGAACATGGATGTCGGCTATCGAGAACATGGCGGAAAAACTAGAAAGC TTCAGCGGCCTGAAACCAGAGGCCAGCGAGCTCCTGCAGTCAGTGCCCTCTATGTTCAGCTTCCGGGCTC CTCCCAACACCCTGCCAGAGAACCTGCTGCGGAAGGGAAAGGAACGCTATACCTGCAGGTACTGTGGTAA GATTTTCCCAAGGTCTGCAAACCTAACACGGCATTTGAGAACCCACACAGGAGAGCAACCTTACAGATGC AAATACTGCGATAGATCATTCAGTATTTCCTCCAACCTGCAGCGACACGTGCGCAACATTCACAACAAGG AGAAGCCATTTAAGTGTCACCTATGTGACAGATGCTTTGGTCAACAAACCAATCTTGACAGACACCTGAA GAAACACGAGAACGGGAACATGTCTGGTACAGCAACATCCTCGCCTCATTCGGAGCTAGAAAGCGCAGGT GCCATCCTGGATGACAAAGAAGATGCTTACTTTACAGAGATCCGCAATTTCATCGGGAATAGCAACCATG GGAGCCAGTCTCCTCGGAACATGGAGGAGAGGATGAATGGCAGTCACTTCAAGGATGAAAAGGCTTTGGC GACCAGCCAAAATTCCGATTTATTGGATGACGAAGAAGTAGAGGATGAGGTGTTGCTGGATGAGGAGGAT GAAGACAATGATATTCCTGGAAAACCCAGAAAGGAGCTGGGGGTAACTAATTTAGATGAGGAAATCCCTG AGGATGACTATGAGGAAGCCGGTGCCCTGGAGATGAGTTGTAAGGTGTCCCCCGTGAGGTATAAAGAGGA AGACTATAAATCTGGCCTTTCTGCTCTAGATCACATAAGGCACTTCACAGATAGCCTCAAAATGAGGGAA ATGGAAGAGAATCAATACACTGACGCTGAGCTGTCCTTTGGTTCTTCTCATGTGCCAGAGGAGCTTAAAC AGCCGTTACACAGAAAGTCCAAATCACAGGCATATGCTATGATGTTGTCACTGTCTGACAAGGATGCCCT CCATCCCACCTCCCACGGTTCCTCCAACGTGTGGCACAGCATGGCAAGAGCTGCAGCGGAATCCAGTGCC ATCCAGTCCATAAGCCATGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212298 representing NM_001106423
Red=Cloning site Green=Tags(s) MTVESTMSVKTVPSRFSRTLATFSDTFDLSMLVPGLMLARSVVKHLPLRQASNNTSSEVCHKSYTQFSNL CRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMGH ANPGLVDYFGTNRHPAGLTFPTAPGFSLSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQSNKCQSPLLTH PQILPATQDILKALSKHPPVGDNKPVELLPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDL ESDLESDKEKCKENGKMFKDKVSPLQNLASINNKKEYSNHSVFSASVEEQTAVSGAVNDSIKAIASIAEK YFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESP FDLTTKRKDEKPLTSGSSKLPATPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGSNMETRPSS DGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFPLPDQRTWMSAIENMAEKLES FSGLKPEASELLQSVPSMFSFRAPPNTLPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRC KYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESAG AILDDKEDAYFTEIRNFIGNSNHGSQSPRNMEERMNGSHFKDEKALATSQNSDLLDDEEVEDEVLLDEED EDNDIPGKPRKELGVTNLDEEIPEDDYEEAGALEMSCKVSPVRYKEEDYKSGLSALDHIRHFTDSLKMRE MEENQYTDAELSFGSSHVPEELKQPLHRKSKSQAYAMMLSLSDKDALHPTSHGSSNVWHSMARAAAESSA IQSISHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106423 |
| ORF Size | 2751 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106423.1, NP_001099893.1 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 2754 bp |
| Locus ID | 294924 |
| MW | 102.1 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212298 | Mecom (untagged ORF) - Rat ecotropic viral integration site 1 (Evi1), (10 ug) |
CNY 11,880.00 |
|
| RR212298L3 | Lenti ORF clone of Mecom (Myc-DDK-tagged ORF) - Rat ecotropic viral integration site 1 (Evi1), (10 ug) |
CNY 13,490.00 |
|
| RR212298L4 | Lenti ORF clone of Mecom (mGFP-tagged ORF) - Rat ecotropic viral integration site 1 (Evi1), (10 ug) |
CNY 14,920.00 |
