Rev1 (NM_001108213) Rat Tagged ORF Clone
CAT#: RR212233
- TrueORF®
Rev1 (Myc-DDK-tagged ORF) - Rat REV1 homolog (S. cerevisiae) (Rev1), (10 ug)
"NM_001108213" in other vectors (3)
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CNY 18,050.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Rev1l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR212233 representing NM_001108213
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGAGGTGGATGGAGGAAGCGAGCAGAGGAGGATGGCTGGGAAAAATGGGGCGGATACATGGCTG CCAAAGTCCAGAAGCTGGAGGAGCAGTTCCGCTCAGATGCGGCTAAGCAGAAGGATGGGACAGCGTCTGC CATATTTAGTGGAGTTGCCATCTACGTTAATGGATATACAGATCCTTCTGCTGAGGAATTAAGAAATCTA ATGATGTTGCATGGAGGTCAATACCATGTGTATTATTCTAGATCCAAAACGACACACATCATTGCTACAA ATCTTCCTAATGCCAAAATTAAAGAACTAAAGGGGGAAAAAGTAATTCGACCTGAATGGATTGTGGAAAG CATCAAAGCTGGGAGACTCTTGTCTTCTGTTCCGTATCAACTATACACCAAGCCATCCACTGCACAGAAG AGTCTCAACTTCACTCCTGTGTGCAAGCCTGAGGAGCCTGTACCAGGTCCAAGCGAGATAGCCAAACAGC TCACCAACAGGGTAAATCACATAATTAAGAAGATTGAGACAGAAAACGAAGTGAAAGCAAATGGCGTGAG CAGCTGGAATGAGGACAGCGCAGATGACGGTTTTAGTTCTGAGGGTCTGGGGCAGACGTTTCCAGGAAGG AAACAGAATGGAGTGATGCATCCCAGAGACACGGCTGTCATTTTTAATGGACATGCTCACAGTTCAAATG GTGCCTTAAAGACGCAGGACTGCTTGGTGCCTGTGGACCACAGTGTTGCTAGCAGGCTCTCTTTAGATTC CACCCAGGAGGAAAAGAGGGCGGAGAAGAGCAGCACTGACTTCAGAGACTGCAGCAGGCAGCACTTGCAG CACAGCACCACAAATGCAGATACTTTGTGCAGTCCACACAGAACTAGTTCTCTCTCACCATCCTTGCACA GTAACACTAAAATCAATGGTGCTCACCACTCCACTGTTCAGGGGCCTTCAAGCACAAAAAGCACTTCTGC TCTGACTCTTAGCAAAGTAGCGCCTTCAGTGCCATCCAAACCATCGGACTGCAGCTTCATTTCAGATTTC TATTCCCGTTCAAGACTGCATCACATCTCTACATGGAAGTGTGAACTGACTGAGTTTGTCAGTGCCCTAC AGAGACAACGTAGTGGTGTCTTTCCAGGAAGGGAAAAGTTAAAAAAAGTGAAAACAGGCAGGTCTTCCCT TGTTGTAACTGACACAGGCGATATGTCAGTGTTGAGCTCACCCAGACCTCAGAGCTGTGTAATGCATGTT GACATGGATTGCTTCTTTGTATCAGTGGGTATACGAAACAGACCAGATCTCAAAGGAAAGCCAGTGGCTG TTACAAGTAACAGAGGCACAGGAAGGGCCCCTTTACGTCCTGGGGCTAACCCACAGCTGGAGTGGCAGTA TTACCAGAATAAACTCCTGAAAGGAAAAGCAGCAGATATACCAGATTCCTCAGTATGGGAGAATCAAGAT TCTGCACAAACAAATGGAATTGATTCTGTTCTGTCAAAAGCTGAAATTGCTTCTTGTAGTTATGAAGCCA GACAAGTTGGCATTAAGAATGGAATGTTTTTTGGACATGCTAAACAGCTGTGTCCTAATCTGCAGGCTGT TCCGTATGACTTCCATGCATGCAGGGAAGTTGCCCAAGCATTGTATGAAACACTGGCAAGCTACACACAC AGCATTGAAGCTGTCAGTTGTGATGAAGCCCTGATTGACGTCACTGACATCCTTGCAGAGACTAAACTTT CTCCTGAGGAGTTTGCAGCTGCCCTGCGTATGGAAATCAAAAACAAGACTCAGTGTGCTGCCTCTGTTGG GATTGGTTCTAATATTCTTCTTGCTAGAATGGCAACTAAAAAAGCAAAGCCGGATGGGCAGTACCACCTG CAGCCAGACGAAGTCGATGACTTCATCAGAGGGCAGCTCGTTACCAGCCTCCCGGGAGTTGGACGTTCAA TGGAATCTAAGCTAGCATCTTTGGGAATTAAGACTTGTGGAGATTTGCAGTGTTTGACCATGGCAAAACT CCAGAAGGAATTTGGCCCCAAGACAGGGCAGATGTTGTATAGGTTCTGCCGAGGTCTGGACGATAGACCT GTTAGAACAGAGAAGGAACGAAAATCTGTTTCAGCCGAGATCAACTACGGAATAAGGTTCACCCAGCCCA GAGAAGCCGAAGCCTTCCTTCTGAGTCTGTCAGGAGAGATTCAAAGCCGACTTGAAGCTGCTGGCATGAA GGGCAAACGCCTGACTCTGAAGATCATGGTACGGAAGCCCGGGGCCCCTATAGAAACTGCAAAATTTGGA GGCCATGGAATTTGTGATAACATCGCCAGGACGGTAACTCTGGACCAGGCAACAGATAGCGCAAAAATAA TTGGGAAAGCTACTCTGAACATGTTTCATACAATGAAATTAAATATTTCAGACATGAGAGGGGTTGGTAT TCAAGTGCATCAGTTGGTTCCTGCTAATTTACATCCTTCCACGTGTTCAAGTCGTCCATCAGCTCAGTCA AGCCTCTTCTCTGGTCTGCCACATTCTGTCCGTGATCTCTTCCAACTTCAGAAAGCTAAGAAACCCACAG AAGAGGAGCACAATGAAGGGCTGGCTGCTGTGGATCTGGAAGTGTCCTCCAACTCTCGAGCTTGTGGTTT ATTGTCACCCCCATCTGCACATCTGGCTACTGTCAGTCCTGATGCTAACAGAGGCGAGTGTTCCAGGAAA TGGAACGGCCTGCATTCTCCTATTGGTGGACAGTCAAGGTTGAACCTCAGTATAGAGGTCCCATCACCGT CCCAGATTGACCAGTCTGTTTTAGAAGCGCTCCCACCTGATATCCGGGAACAAGTAGAACAAGCTTATGC TGCTCAACAAGGAGAGCCGCGTGGCTCTAAGAAGAAAGAACCAGTAAATGGCTGCAGTTCAGGGGTATTG CCACATCCTGTTGGCACAGTTTTGTTACAAATACCAGAGCCTCAAGAACCTAATAACAGTGACACCAGAA TCAGCGTGATTGCCCTTCCAGCGTTCTCACAAGTGGACCCTGATGTCTTTGCTGCCCTTCCTGCTGAGCT TCAGAAGGAGCTGAAAGCAGCGTATGATCAAAGACAAAGGCAGGGCGAGGATACCACTCAGCAGCAGCCA GCTAGTACAAGTGTGCCAAAGAATCCTTTACTTCAGCTAAAGCCACCAGCAATGAAAGACAAGAGAAACA AGAGGAAGAACCCCATTGGTTCCCCCAGGAAGATTCAGAGTCTGCCAGGGACCTCTGGGAGCCCTCAGAA GTTAATGGATGGTTTTCTACAACATGAAGAGAGACCCCTGGTAACTGGAACAAAGACCGACTGTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212233 representing NM_001108213
Red=Cloning site Green=Tags(s) MRRGGWRKRAEEDGWEKWGGYMAAKVQKLEEQFRSDAAKQKDGTASAIFSGVAIYVNGYTDPSAEELRNL MMLHGGQYHVYYSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSSVPYQLYTKPSTAQK SLNFTPVCKPEEPVPGPSEIAKQLTNRVNHIIKKIETENEVKANGVSSWNEDSADDGFSSEGLGQTFPGR KQNGVMHPRDTAVIFNGHAHSSNGALKTQDCLVPVDHSVASRLSLDSTQEEKRAEKSSTDFRDCSRQHLQ HSTTNADTLCSPHRTSSLSPSLHSNTKINGAHHSTVQGPSSTKSTSALTLSKVAPSVPSKPSDCSFISDF YSRSRLHHISTWKCELTEFVSALQRQRSGVFPGREKLKKVKTGRSSLVVTDTGDMSVLSSPRPQSCVMHV DMDCFFVSVGIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKLLKGKAADIPDSSVWENQD SAQTNGIDSVLSKAEIASCSYEARQVGIKNGMFFGHAKQLCPNLQAVPYDFHACREVAQALYETLASYTH SIEAVSCDEALIDVTDILAETKLSPEEFAAALRMEIKNKTQCAASVGIGSNILLARMATKKAKPDGQYHL QPDEVDDFIRGQLVTSLPGVGRSMESKLASLGIKTCGDLQCLTMAKLQKEFGPKTGQMLYRFCRGLDDRP VRTEKERKSVSAEINYGIRFTQPREAEAFLLSLSGEIQSRLEAAGMKGKRLTLKIMVRKPGAPIETAKFG GHGICDNIARTVTLDQATDSAKIIGKATLNMFHTMKLNISDMRGVGIQVHQLVPANLHPSTCSSRPSAQS SLFSGLPHSVRDLFQLQKAKKPTEEEHNEGLAAVDLEVSSNSRACGLLSPPSAHLATVSPDANRGECSRK WNGLHSPIGGQSRLNLSIEVPSPSQIDQSVLEALPPDIREQVEQAYAAQQGEPRGSKKKEPVNGCSSGVL PHPVGTVLLQIPEPQEPNNSDTRISVIALPAFSQVDPDVFAALPAELQKELKAAYDQRQRQGEDTTQQQP ASTSVPKNPLLQLKPPAMKDKRNKRKNPIGSPRKIQSLPGTSGSPQKLMDGFLQHEERPLVTGTKTDCY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001108213 |
ORF Size | 3357 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001108213.1, NP_001101683.1 |
RefSeq Size | 4092 bp |
RefSeq ORF | 3360 bp |
Locus ID | 316344 |
MW | 122.4 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN212233 | Rev1 (untagged ORF) - Rat REV1 homolog (S. cerevisiae) (Rev1), (10 ug) |
CNY 18,050.00 |
|
RR212233L3 | Lenti ORF clone of Rev1 (Myc-DDK-tagged ORF) - Rat REV1 homolog (S. cerevisiae) (Rev1), (10 ug) |
CNY 19,570.00 |
|
RR212233L4 | Lenti ORF clone of Rev1 (mGFP-tagged ORF) - Rat REV1 homolog (S. cerevisiae) (Rev1), (10 ug) |
CNY 21,570.00 |