Ttbk2 (NM_001107766) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ttbk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212013 representing NM_001107766 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGT TAAGAAAGATTGGGGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGT GGCACTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAA CTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGTTGTGGGAGAAATGACCGTTTCAACTACGTGGTCA TGCAATTGCAGGGTCGGAATCTGGCAGATCTTCGACGTAGCCAATCTCGGGGCACATTCACTATTAGCAC TACTCTTCGTCTCGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGA GACATCAAACCGTCAAATTTCGCCATGGGACGCTTTCCCAGTACATGTAGAAAATGTTTTATGCTTGATT TTGGCTTGGCCCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTCCG AGGGACAGTTCGCTATGCATCCATCAATGCTCATCGGAACAGGGAGCAAGTAGGTTCCATTAAGGAGAGG TATGACCATAGGCTCATGTTGAAACACCTCCCTCCAGAATTCAGCACCTTTCTTGACCATATTTCCTCTT TGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTTGACAATAGCATCAAGACCTTTGG AGTAATTGAAAGTGACCCTTTTGACTGGGAGAAGAGTGGAACTGACGGCTCCCTAACGACCACCACCACC TCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCAATCGGAATTGCAAATGCCACCCCTATCC CAGGAGACTTGCTTCGAGAAAATACAGACGAAGTGTTTCCAGATGAGCAGCTTAGTGATGGGGAGAATGG AATCCCTGCTGGTGTGTCCCCAGATAAATTGCCTGGATCTCTGGGGCACCCTCGCCCTCAGGAAAAGGAT GTCTGGGAAGAGATGGACATCAACAAGAACAAGATAAAGCTCGGAATTTGTAAAGCAGCTACTGAAGAAG AAAATAGCCATGGTCAAGTGAATGGAATTCTTAATGCTCCAAGCCTTGGCTCACCAATTCGTGTCCGATC AGAGATTACTCAGCCAGACAGAGATGTTCCTTTAGTAAGGAAGTTACGTTCCATTCACAGCTTTGAGCTG GAAAAACGTCTGACTCTCGAGCCAAAGCCAGATACTGATAAGTTTCTTGAGACCTGCATGGAGAAAATGC AGAAAGATTCCAGTGCAGGAAAGGAACCTGTTCTCCCTGCTCTGCCTCATAAGCCTTGTGTCCCTGCTGT GCCCCGCACTGACCACGTCTGGCACTATGATGAAGAATATCCTCCTGATGCCTCCAAGCCTGCCTCTGCC AACACCCCAGAGCAGGCCGATGGTGGTGGCAGCCACGGATTTATAGCTGTTAACTTAAGCTCTTGTAAGC AGGAGGTTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAAGACCTTCGGGACTTTAGGACAAATGA GGTGTTAGGCCATAAAACAACTGGAAGCCCTTCAGATGAGGAGCCGGAAGTGCTTCAGGTCCTTGAGGGG TCGCCTCAAGATGAAAAGATCCAAGTAGGTCCTTGGACTGACAACCAGCACTTAAAGAAGGAAAGCTCAG GTGTGATTTTAGCCCTTTCTGCTGAATGCCCTGCTACTGCTGCTTCAGAACTGTACACAGATAGGCTAGA CCTCCAGGCTGGAGCTGCAAGTCAGTTCATCACAGTGACTCCCACAAGTCCCATGGAGGCACAAGCAGAA GGACCCCTGACTGCGATTACAATTCCTCGACCTTCTGTGGCATCAACACAGTCAACTTCAGGAAGTTTTC ACTATGGCCCACAACCAGAGAAGAAAGATCTTCAGCCCTTGGAGCCCACCGTAGAACTCTTCTCTCCAAG GGAGAACTTCTCGGGCTTGGCTGTGACAGAGGGTGAACTAGGAAGCGGAGGAAGCAGAGTGGATTTGGGA CTTCAGATAGATCACATTGGTCATGGCATGTTACCCAACATGAAAGATGGTGACGCGTCTCAAGACTTAG GACCAAGAGACCTTCCTGACAGTAATAGATTAGTTGTGAAAGAATTTGAAAATCTCCCTGGAGAAACAGA AGAGAAAAGCATTCTTCTGGGCTCAGATAATGAAGATGAGAAGCTAAATAAAGGGCAACACTGTATTGAG ATCTCTCTCCCAGGAGATTTAGTGACCACAGAAAGGGATCAGTCAGCTACCACAGAACCTCTGGATGTGT CAAAGACACAGAATTTCAGTGTGGTGCCTAATCAGGACAAAAACCATGAGATAATGAAGCTTTTGGCAGT TGGAACGTCAGAAATGTCTCCACAAGCCATTGACCCACATGCTGAAGGGCAGATAGGCCAGGTGGCAACA GTGCAAAAACACAAGCTATTTAAAGATGACACTAAGAGTGAGAGCTTGCCAGGCCAGCAGGGAGACCTTG CTACTTTTTTGCACCAAGAGGGTAAGAGAGAGAAAGTTGCCCCTAGAAATGGAGAGCTCTATCACTGTGT CTCAGAGAGTGAGCATGGGCCTCCTACCCGGAAGGACATGCTTCGGTCGTCCTTGGTGACCAGGCACAGC CGGATCCCTGTTTTAGCACAAGAAATAGACTCAACTTTTGAATCATCCTCTGCAGTCTCTGCAAAAGAAA AGCTTTTACAGAAGAAAGCCTATCAGCCAGAAATAGTCAAACTTCTGGTAGAAAAAAGGCAATTCAAGTC TTTCCTCGGGGACCTCTCAAGTGCCTCTGAGAAGCTGATGGAGGAGAAGCTAGCTGCTGCTGCAGTTCCC TTTTCTGAGGAGGAAGTCTTCGCCCCCTTTTCCAGACTGGCAGTAGACCCCCAGCTGAGTAGGTCAGTTG AAGATGGCTTTCTGTCACCCATCGTCTCCCAGTCTAGAAAGAGCAAGATTCCGAGGCCAGTATCCTGGGT CAGCACAGATCAAATCAATAGCTCAGCTTCACCTCAGTTCTTGCCTCGGCCGCCACCGGGAAAGCCACCA GTAAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTTCTAGGGAGTAGTAACTCTGATTCAGACC TTTTCTCTCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAATCCCGGAGTACTACACAATGCAAGAG CCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTACCTCGAAGGAGTCCCAGTGCC TCTCCTCGAAGCTCTTCCTTGCCTCGAACATCTAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATG ACCAGAGGAGTTCTTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCATCATCCTC CAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCCCCAAAGGATCTGGCAGCCTCCAC CACCACTCAGCCAGCTCTAAAACTCCCCCAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212013 representing NM_001107766 Red=Cloning site Green=Tags(s) MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKK LQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGKQILESIESIHSVGFLHR DIKPSNFAMGRFPSTCRKCFMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREQVGSIKER YDHRLMLKHLPPEFSTFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKSGTDGSLTTTTT SATPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSDGENGIPAGVSPDKLPGSLGHPRPQEKD VWEEMDINKNKIKLGICKAATEEENSHGQVNGILNAPSLGSPIRVRSEITQPDRDVPLVRKLRSIHSFEL EKRLTLEPKPDTDKFLETCMEKMQKDSSAGKEPVLPALPHKPCVPAVPRTDHVWHYDEEYPPDASKPASA NTPEQADGGGSHGFIAVNLSSCKQEVDSKEWVIVDKEQDLRDFRTNEVLGHKTTGSPSDEEPEVLQVLEG SPQDEKIQVGPWTDNQHLKKESSGVILALSAECPATAASELYTDRLDLQAGAASQFITVTPTSPMEAQAE GPLTAITIPRPSVASTQSTSGSFHYGPQPEKKDLQPLEPTVELFSPRENFSGLAVTEGELGSGGSRVDLG LQIDHIGHGMLPNMKDGDASQDLGPRDLPDSNRLVVKEFENLPGETEEKSILLGSDNEDEKLNKGQHCIE ISLPGDLVTTERDQSATTEPLDVSKTQNFSVVPNQDKNHEIMKLLAVGTSEMSPQAIDPHAEGQIGQVAT VQKHKLFKDDTKSESLPGQQGDLATFLHQEGKREKVAPRNGELYHCVSESEHGPPTRKDMLRSSLVTRHS RIPVLAQEIDSTFESSSAVSAKEKLLQKKAYQPEIVKLLVEKRQFKSFLGDLSSASEKLMEEKLAAAAVP FSEEEVFAPFSRLAVDPQLSRSVEDGFLSPIVSQSRKSKIPRPVSWVSTDQINSSASPQFLPRPPPGKPP VRPGVEARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKSRSTTQCKSPGSPHNPKTPPKSPVVPRRSPSA SPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPPSHSGSSSSRRSCQQEHCKPSKNGPKGSGSLH HHSASSKTPPGKSKPASKLSR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107766
ORF Size	3633 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107766.1, NP_001101236.1
RefSeq Size	4273 bp
RefSeq ORF	3636 bp
Locus ID	311349
MW	132.6 kDa

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