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Mgea5 (NM_131904) Rat Tagged ORF Clone
CAT＃: RR211943
- TrueORF®
Mgea5 (Myc-DDK-tagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), (10 ug)
"NM_131904" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ncoat; O-GLCNACASE; OGA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211943 representing NM_131904
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGAAGGAGAGTCAAGCGGCGCTGGAGGAGCGGGAGAGTGAGCGCAACGCCAATCCTGCCTCCG TCTCGGGGGCTTCGTTGGAGCCGTCTGCAGCTCCGGCCCCCGGAGAAGACAACCCCTCGGGAGCTGGGGC GGCGGCCGGGACCGGGGCGGCCGGCGGGGCCCGCAGGTTCCTGTGCGGTGTAGTGGAAGGGTTTTATGGA AGACCTTGGGTTATGGAGCAGAGAAAAGAGCTCTTTAGAAGGCTTCAGAAATGGGAATTAAATACATACT TGTATGCTCCGAAGGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAAGAAGCTGA GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAATTCATCTATGCTATTTCTCCAGGA TTAGATATCACTTTTTCTAACCCAAAGGAAGTATCAACATTGAAACGCAAATTGGACCAGGTCTCCCAGT TTGGGTGCAGGTCATTTGCTTTGCTTTTTGACGATATTGATCATAACATGTGTGCAGCAGACAAAGAAGT GTTCAGTTCTTTTGCACATGCACAAGTTTCCATCACAAATGAAATCTATCAGTACCTGGGAGAGCCGGAA ACCTTCCTCTTCTGTCCCACAGAATACTGCGGCACTTTCTGTTATCCAAGTGTATCTCAGTCTCCTTACT TGAGGACTGTGGGTGAAAAGCTGCTACCTGGAATTGAGGTGCTTTGGACAGGTCCAAAAGTTGTTTCAAA AGAAATTCCAGTAGAATCTATTGAAGAAGTTTCTAAGATCATTAAGAGAGCCCCAGTGATCTGGGATAAC ATTCATGCTAATGACTATGATCAGAAGAGGCTGTTTCTGGGTCCCTACAAAGGAAGATCCACAGAGCTCA TCCCACGGTTGAAGGGAGTGCTGACTAATCCAAATTGTGAATTTGAAGCCAACTATGTTGCCATCCACAC CCTTGCCACTTGGTACAAGTCAAACATGAACGGAGTGAGGAAGGACGTGGTGATGACTGACAGTGAAGAC AGTACTGTCTCAATACAGATAAAGTTAGAAAATGAAGGCAGTGATGAAGACATTGAGACTGATGTGCTCT ACAGTCCACAGATGGCTTTAAAGCTGGCCTTAACTGAGTGGCTGCAGGAGTTCGGTGTACCGCATCAGTA CAGCAGTAGACAGGTTGCACACAGTGGAGCTAAGACAAGCGTAGTGGACGGGACTCCCTTGGTTGCAGCA CCTTCTTTAAATGCCACAACTGTAGTTACAACAGTTTACCAGGAGCCCATTATGAGCCAGGGAGCAGCTT TGAGTGGTGAGCCCTCAGCTCTGACCAAGGAAGAAGAAAAGAAACAGCCAGATGAGGAACCCATGGATAT GGTGGTAGAGAAGCAAGAAGAGTCAGAGCACAAGAGTGACAACCAGATACTAACTGAGATCGTCGAAGCT AAAATGGCAGAGGAGTTGAAACCAATGGACACTGATAAGGAAAGCATAGCCGAGTCAAAATCCCCAGAGA TGTCTATGCAGGAAGACTGCATTAACGACATCGCTCCTATGCAGACAGACGAGCAAGCAAACAAAGAGCA GTTTGTGCCAGGTCCAAATGAAAAACCTTTGTACGCTGCAGAACCAGTGACTCTGGAGGATTTACAGTTA CTTGCTGATCTCTTCTACCTTCCTTATGAGCATGGACCCAAAGGAGCACAGATGCTACGGGAGTTCCAGT GGCTTCGAGCAAACAGTAGTGTTGTCAGTGTCAATTGCAAAGGGAAAGACTCTGAAAAAATTGAAGAATG GCGGTCACGAGCAGCCAAGTTTGAAGAAATGTGCGCACTAGTGATGGGGATGTTCACCCGGCTCTCCAAC TGTGCCAACAGGACAATCCTTTATGACATGTACTCCTATGTGTGGGATATCAAGAGTATAATGTCTATGG TGAAGTCTTTTGTGCAGTGGTTAGGGTGTCGTAGTCATTCTTCAGCACAGTTCTTAATTGGAGACCAAGA ACCCTGGGCCTTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGAGCAAATGAT CTCTTTTTCCAACCACCCCCACTGACTCCTACCTCCAAGGTTTATACTATCAGACCTTATTTCCCTAAAG ATGAGGCTTCCGTGTACAAGATTTGCAGAGAAATGTATGATGATGGAGTGGGTTTACCTTTTCAAAGTCA GCCTGACCTTATTGGAGACAAGTTAGTAGGAGGACTGCTTTCCCTCAGCCTGGATTACTGTTTTGTCCTC GAAGATGAAGATGGTATTTGTGGTTATGCCTTAGGCACTGTAGATGTGACCCCCTTCATTAAAAAGTGCA AAATTTCCTGGATTCCCTTCATGCAGGAGAAGTATACTAAGCCAAATGGAGACAAAGAGCTCTCAGAAGC TGAGAAAATAATGTTGAGTTTCCATGAAGAGCAGGAGGTATTACCAGAAACTTTCCTTGCCAACTTCCCT TCTCTGATAAAAATGGATATTCATAAAAAGGTCACTGACCCAAGTGTTGCCAAAAGCATGATGGCTTGCC TCCTGTCTTCACTAAAGGCCAATGGCTCCCGGGGGGCTTTCTGTGAAGTAAGACCAGATGATAAAAGGAT TCTGGAATTTTACAGCAAGTTAGGCTGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTT ATACTTGGTCGAAGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211943 representing NM_131904
Red=Cloning site Green=Tags(s) MVQKESQAALEERESERNANPASVSGASLEPSAAPAPGEDNPSGAGAAAGTGAAGGARRFLCGVVEGFYG RPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPG LDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPE TFLFCPTEYCGTFCYPSVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDN IHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSED STVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAA PSLNATTVVTTVYQEPIMSQGAALSGEPSALTKEEEKKQPDEEPMDMVVEKQEESEHKSDNQILTEIVEA KMAEELKPMDTDKESIAESKSPEMSMQEDCINDIAPMQTDEQANKEQFVPGPNEKPLYAAEPVTLEDLQL LADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSN CANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGAND LFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVL EDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFP SLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVV ILGRSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_131904 |
| ORF Size | 2748 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_131904.1, NP_571979.1 |
| RefSeq Size | 2751 bp |
| RefSeq ORF | 2751 bp |
| Locus ID | 154968 |
| UniProt ID | Q8VIJ5 |
| MW | 102.9 kDa |
| Gene Summary | plays a role in insulin secretion; may be involved in secretion of other glucose regulated hormones [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211943 | Mgea5 (untagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), (10 ug) |
CNY 11,780.00 |
|
| RR211943L3 | Lenti ORF clone of Mgea5 (Myc-DDK-tagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), (10 ug) |
CNY 13,490.00 |
|
| RR211943L4 | Lenti ORF clone of Mgea5 (mGFP-tagged ORF) - Rat meningioma expressed antigen 5 (hyaluronidase) (Mgea5), (10 ug) |
CNY 14,920.00 |
