Mtmr3 (NM_001012038) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211921 representing NM_001012038 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCATAGCCTTGAGTGCATCCAGGCCAATCAGATCTTTCCCAGGAAGCAGCTGATTCGGGAGGATG AAAATCTTCAGGTTCCTTTCCTTGAACTCCATGGAGAGAGCACAGAGTATGTGGGCCGTGCTGAGGATGC CATCATTGCCCTCTCTAATTACAGACTTCACATCAAGTTTAAGGAGTCTCTTGTGAATGTTCCATTACAG CTTATAGAAAGCGTTGAATGCCGTGATATATTTCAGCTTCACTTGACATGCAAAGACTGCAAAGTTATCA GGTGTCAGTTCCCAACCTTTGAGCAGTGTCAAGATTGGCTTAAGAGATTAAACAATGCAATCCGACCACC TGGAAAAATAGAAGATCTCTTCTCATTTGCATACCATGCTTGGTGCATGGAGGTCTATGCCAGTGAAAAA GAGCAGCATGGAGACCTGTGCAGACCAGGGGAACATGTAACGTCAAGGTTTAAGAATGAAGTGGAGCGGA TGGGTTTTGATATGAACAATGCCTGGAGGATTTCCAACATCAATGAGAAATACAAATTATGTGGTAGTTA TCCACAAGAGCTCATAGTACCTGCCTGGATCACTGACAAAGAACTAGAAAGCGTGGCAGGCTTCAGATCC TGGAAGCGCATCCCCGCCGTCATCTACAGGCACCAGAGCAATGGAGCCGTCATTGCCCGCTGCGGACAGC CCGAGGTTAGCTGGTGGGGCTGGCGGAATGCTGATGATGAACATCTGGTGCAGTCAGTGGCCAAGGCTTG TGCTTCTGACTCTCAGTCAAGTGTTGGCAAGGTTTCCACTCGGAACAGTTGTCGGGGCTTTCCCAATGCA GGGGATCTTTCTGATGTGGAGTTCGATGCCTCTCTGTCAAATGCTTCAGGAACAGAAAGTTTAGCTCTCC AGCCACAGAAGCTTTTGATCTTGGACGCACGCTCCTATGCAGCAGCTGTGGCAAATCGAGCCAAAGGAGG GGGCTGTGAGTGTCCAGAGTATTATCCAAACTGTGAAGTTGTGTTTATGGGAATGGCAAACATACATTCT ATCCGGAGGAGTTTCCAGTCTCTGCGACTGCTGTGCACACAGATGCCAGATCCTGGAAATTGGCTTTCTG CTCTTGAAAGTACGAAGTGGCTCCACCACCTGTCTGTGCTTCTGAAGTCGGCACTTCTCGTGGTGCATGC TGTGGACCGAGATCAGCGACCAGTGCTGGTGCACTGCTCAGATGGCTGGGATCGCACTCCCCAAATCGTG GCCCTGGCTAAGCTTCTGCTGGACCCTTATTACCGAACCGTAGAGGGTTTCCAAGTCCTTGTGGAGATGG AATGGCTGGATTTTGGCCATAAGTTTGCTGATCGGTGTGGTCATGGGGAGAACTCAGATGACCTGAATGA GCGTTGCCCAGTGTTTCTCCAATGGCTTGACTGTGTTCATCAACTCCAGAGGCAATTTCCTTGCTCTTTT GAGTTCAACGAAGCATTCCTTGTGAAGCTGGTTCAGCACACCTATTCCTGTCTCTTTGGGACATTCCTGT GCAACAACGCCAAGGAGAGGGGGGAGAAGCAGACTCAGGAACGGACATGCTCGGTGTGGTCGCTTCTGCG GGCCGGCAACAAGGCCTTCAAAAACCTGCTATATTCATCCCAGTCAGAAGCCGTGCTGTACCCTGTGTGC CATGTGCGGAATCTGATGCTGTGGAGTGCAGTGTACCTTCCCTGCCCGTCCCCATCCACCCCCACAGACG ACAGCTGTGCACCATACCCAGCCCCAGGCACCAGCCCTGATGAGCCTCCACTGAGCCGGCTACCCAAGAC TAGATCATTTGACAATCTGACCACAACCTGTGACAACATGGTGCCGCTGGCCAGCCGGCGCAGCAGTGAC CCCAGCCTGAATGAGAAGTGGCAGGAGCACGGGCGCTCGCTGGAGTTGAGCAGCTTTGCTGGCTCTGGGG AAGAGGTGCCTGCCATAGACAGCCTGCGCAGGCCCAGCAGGCTGCTCGGAGGAGCCGAGCTATCTGTGGC AGCTGGTGTGGCTGAAGGGCAGATGGAGAATATTTTGCAAGAGGCCACCAAAGAGGAGAGTGGGGTAGAA GAGCCCACCCACAGGGAGCACACTGAGGTGCCAGAGGTCAAAGAGGAGGCACCCCTAGCAAAAGAGAGTA GGACGGCAGCTCAGGGCTCAGGTGTACTTTACCAAGAACCACAGCTGGATGATGCTACTCTAAGGAGCCA TTTAGGCCCCAGCCTGTCTTCGTTCTCCCAGGGTATTCCTGAGCATCGGGAGGTGGGGCACAGTGTTCTC TCTAGTTCACTCCCAGCTTCTCTCAGGGGAGAGGACTCCCAGGAGGTCCCTGTAGAACAGCCTCAAGTAG AGAACATTGCAGAGGACAGGGAGAATGTAGTCCCTGCTGTCCCAGTAGATGTAAAAATTGGCCTTGGTAC CTCAGAGTCTAGTCCTCTGCTGCCTTCCCAAGTCCCATTCGAGACAAGAGGACCACACATGAACAACTCC GTGCACATGTTATTAGAAGATAAGGTGAAGTCCGAGAGTGGGCCCCAGCTCCATCACAGACCCTGCCTGG CAAGCAGTGGTAGATTCAGTGGCAAGGACATGCTTCCTATAGCACCAGAGCCCAGGTCTGCTGAGAGGCC CCAGTGGGACTCTGTGCTACACAGGACTTCTTCCCCTGGCAACACCCTCAGCCTGATGATGCAGGCTCCT TGTGCCTTGCCATTAGACAAATGTAGACAGAGAATTGTGTGCAACGGTGCCCTAGAGACTGAAAACAAGG CCTCAGAACAGCCTGCAGGGTTTGACACCCTTCAGAAGTACCCCACACCCAATGGGCATTGTGCCAATGG GGAGACTGGGAGGAGTAAGGACTCACTGAGCCACCAGCTGTCTGCCACAAGCTATAGCTCTGCTCACTCG TGCTCAAGGAACTTGCACCACAAGTGGCTGAATAGCCACTCGGGGAGGCCATCCACTACCAACAGCCCTG AGCAGCCTTCCCGCAGCCACCTGGATGATGACGGCATGCCTGTGTACACAGACACGATCCAACAGCGCCT GCGACAGATAGAGTCTGGTCACCAGCAGGAAGTGGAGACCTTGAAGAAACAAGTCCAGGAACTGAAGAGT CGCCTGGAGAGCCAGTACCTGACCAGCTCTCTGCGCTTCAATGGGGACTTTGGAGATGAAGTGACTTCAA TCCCCGACTCGGAAAGCAATCTGGATCAGAACTGTTTGTCTCGCTGCAGCACAGAGATTTTCTCTGAAGC CAGCTGGGAGCAGGTGGATAAACAGGACACAGAGATGACCCGTTGGCTACCTGACCACTTGGCTGCCCAC TGCTATGCCTGTGACAGTGCCTTCTGGCTTGCCAGTAGGAAGCACCACTGCAGGAATTGTGGGAACGTAT TCTGCTCCAGTTGTTGTAACCAGAAGGTTCCAGTTCCCAGCCAGCAGCTCTTTGAACCCAGTCGAGTGTG CAAGTCTTGCTATAGCAGCCTCCATCCCACAAGCTCCAGCATTGACCTTGAACTGGATAAGCCTATTGCT GCCACTTCAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211921 representing NM_001012038 Red=Cloning site Green=Tags(s) MRHSLECIQANQIFPRKQLIREDENLQVPFLELHGESTEYVGRAEDAIIALSNYRLHIKFKESLVNVPLQ LIESVECRDIFQLHLTCKDCKVIRCQFPTFEQCQDWLKRLNNAIRPPGKIEDLFSFAYHAWCMEVYASEK EQHGDLCRPGEHVTSRFKNEVERMGFDMNNAWRISNINEKYKLCGSYPQELIVPAWITDKELESVAGFRS WKRIPAVIYRHQSNGAVIARCGQPEVSWWGWRNADDEHLVQSVAKACASDSQSSVGKVSTRNSCRGFPNA GDLSDVEFDASLSNASGTESLALQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMANIHS IRRSFQSLRLLCTQMPDPGNWLSALESTKWLHHLSVLLKSALLVVHAVDRDQRPVLVHCSDGWDRTPQIV ALAKLLLDPYYRTVEGFQVLVEMEWLDFGHKFADRCGHGENSDDLNERCPVFLQWLDCVHQLQRQFPCSF EFNEAFLVKLVQHTYSCLFGTFLCNNAKERGEKQTQERTCSVWSLLRAGNKAFKNLLYSSQSEAVLYPVC HVRNLMLWSAVYLPCPSPSTPTDDSCAPYPAPGTSPDEPPLSRLPKTRSFDNLTTTCDNMVPLASRRSSD PSLNEKWQEHGRSLELSSFAGSGEEVPAIDSLRRPSRLLGGAELSVAAGVAEGQMENILQEATKEESGVE EPTHREHTEVPEVKEEAPLAKESRTAAQGSGVLYQEPQLDDATLRSHLGPSLSSFSQGIPEHREVGHSVL SSSLPASLRGEDSQEVPVEQPQVENIAEDRENVVPAVPVDVKIGLGTSESSPLLPSQVPFETRGPHMNNS VHMLLEDKVKSESGPQLHHRPCLASSGRFSGKDMLPIAPEPRSAERPQWDSVLHRTSSPGNTLSLMMQAP CALPLDKCRQRIVCNGALETENKASEQPAGFDTLQKYPTPNGHCANGETGRSKDSLSHQLSATSYSSAHS CSRNLHHKWLNSHSGRPSTTNSPEQPSRSHLDDDGMPVYTDTIQQRLRQIESGHQQEVETLKKQVQELKS RLESQYLTSSLRFNGDFGDEVTSIPDSESNLDQNCLSRCSTEIFSEASWEQVDKQDTEMTRWLPDHLAAH CYACDSAFWLASRKHHCRNCGNVFCSSCCNQKVPVPSQQLFEPSRVCKSCYSSLHPTSSSIDLELDKPIA ATSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001012038
ORF Size	3582 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001012038.1, NP_001012038.1
RefSeq Size	5733 bp
RefSeq ORF	3585 bp
Locus ID	305482
UniProt ID	Q5PQT2
MW	133.5 kDa
Gene Summary	Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues (By similarity).[UniProtKB/Swiss-Prot Function]

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