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Capn3 (NM_017117) Rat Tagged ORF Clone
CAT＃: RR211917
- TrueORF®
Capn3 (Myc-DDK-tagged ORF) - Rat calpain 3 (Capn3), (10 ug)
"NM_017117" in other vectors (3)
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CNY 10,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lp82; Lp84; Lp85; p94 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211917 representing NM_017117
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGACCGTTATTAGTCCAACTGTGGCCCCAAGGACAGGAGCTGAGCCCAGGTCCCCAGGGCCAGTTC CTCACCCAGCTCAAGGCAAGACCACTGAGGCTGGAGGTGGACACCCGGGTGGCATCTATTCAGCCATCAT CAGCCGCAATTTTCCGATCATTGGTGTGAAAGAGAAGACATTCGAGCAGCTCCACAAGAAGTGCCTAGAG AAGAAAGTTCTTTACCTGGATCCCGAGTTCCCACCGGATGAGACCTCTCTCTTTTACAGCCAGAAGTTCC CCATCCAGTTCGTCTGGAAGAGACCTCCGGAAATTTGTGAGAATCCCCGATTTATCATTGGTGGAGCCAA CAGGACTGACATCTGCCAAGGAGATCTAGGGGACTGCTGGCTTCTTGCAGCCATTGCCTGTCTGACCCTG AATGAGCGACTGCTTTTCCGAGTTATACCTCATGATCAAAGTTTCACTGAAAACTACGCAGGGATCTTCC ACTTCCAGTTCTGGCGCTATGGAGACTGGGTAGATGTGGTTATTGACGACTGTCTGCCGACATACAACAA CCAGCTGGTCTTCACCAAATCCAACCACCGCAATGAGTTCTGGAGTGCTCTACTGGAGAAAGCATATGCC AAGCTCCATGGTTCCTATGAAGCTCTGAAAGGTGGGAACACCACAGAAGCCATGGAGGACTTCACAGGAG GGGTGACAGAGTTTTTTGAGATCAAGGATGCTCCGAGTGACATGTACAAGATCATGAGGAAAGCTATCGA GAGAGGCTCCCTCATGGGCTGCTCCATTGATGATGGCACCAACATGACTTATGGAACCTCTCCTTCTGGT CTGAACATGGGGGAATTGATTGCGCGGATGGTGAGAAATATGGATAACTCGCTGCTCAGAGACTCAGACC TGGACCCCAGGGCCTCAGATGACAGACCGTCACGGACAATTGTTCCGGTGCAGTATGAAACAAGAATGGC CTGTGGACTGGTGAAAGGGCACGCCTATTCAGTCACTGGGCTGGAGGAGGCCCTGTTCAAAGGCGAGAAG GTGAAGCTGGTGCGGCTGCGGAACCCCTGGGGCCAGGTGGAGTGGAACGGCTCTTGGAGTGATGGTTGGA AGGACTGGAGCTTTGTGGACAAAGACGAGAAGGCCCGTCTGCAGCACCAGGTCACCGAGGATGGAGAGTT CTGGATGTCATATGATGACTTTGTCTACCATTTCACAAAGCTGGAGATCTGCAACCTCACAGCTGATGCC CTGGAGTCCGATAAGCTTCAGACCTGGACAGTGTCTGTAAATGAGGGCCGCTGGGTGAGGGGCTGTTCTG CTGGAGGCTGCCGGAACTTCCCAGACACTTTCTGGACCAACCCGCAGTACCGTCTCAAGCTCCTGGAGGA GGATGATGACCCTGATGACTCTGAGGTGATTTGCAGCTTCCTCGTGGCTCTGATGCAGAAAAATCGGCGC AAGGACCGGAAGCTGGGGGCCAACCTCTTCACCATTGGCTTCGCTATCTACGAGGTTCCCAAAGAGATGC ACGGGAATAAGCAACACCTGCAGAAGGACTTCTTCTTGTACAATGCCTCCAAGGCCAGGAGCAAAACCTA CATCAACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCGCCCAGCGAGTATGTCATTGTCCCCTCCACT TACGAGCCCCATCAGGAGGGGGAATTCATCCTCCGGGTCTTCTCTGAAAAGAGGAATCTCTCTGAGGAAG CTGAGAATACAATCTCTGTGGACCGGCCAGTGAAAAAGAAAAAAAACAAGCCCATCATCTTCGTTTCAGA CAGAGCAAACAGCAACAAGGAGCTGGGTGTGGACCAGGAGGCAGAGGAGGGCAAAGACAAAACAGGGCCG GATAAACAAGGGGAAAGCCCACAGCCACGGCCTGGCCACACAGACCAGGAGAGTGAGGAGCAGCAGCAAT TCCGGAACATCTTCAGGCAGATTGCAGGCGACGACATGGAGATCTGTGCGGATGAACTCAAGAATGTCCT TAATACGGTGGTGAACAAACACAAGGACCTGAAGACACAAGGGTTCACTCTGGAGTCCTGCAGAAGCATG ATAGCTCTCATGGATACAGATGGCTCTGGGAGACTGAATCTTCAAGAGTTCCATCACCTCTGGAAAAAGA TCAAGGCCTGGCAGAAAATCTTCAAACACTATGACACTGACCATTCTGGTACCATCAATAGCTATGAGAT GCGAAATGCAGTCAATGATGCAGGCTTCCATCTCAACAGCCAACTCTATGACATCATCACCATGCGCTAC GCAGACAAACACATGAACATCGACTTTGACAGCTTCATCTGCTGCTTCGTCAGGCTGGAAGGGATGTTCA GAGCTTTTCACGCATTTGACAAGGATGGAGATGGCATCATCAAACTGAACGTACTTGAGTGGCTGCAGCT TACCATGTATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211917 representing NM_017117
Red=Cloning site Green=Tags(s) MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPGGIYSAIISRNFPIIGVKEKTFEQLHKKCLE KKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWLLAAIACLTL NERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYA KLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSG LNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEK VKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADA LESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRR KDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPST YEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEGKDKTGP DKQGESPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSM IALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRY ADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017117 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017117.1, NP_058813.1 |
| RefSeq Size | 3138 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 29155 |
| UniProt ID | P16259 |
| MW | 94.1 kDa |
| Gene Summary | calcium-activated protease; alternative splicing produces different protein products; may be involved in astrocyte plasticity and in eye development and pathology [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211917 | Capn3 (untagged ORF) - Rat calpain 3 (Capn3), (10 ug) |
CNY 10,550.00 |
|
| RR211917L3 | Lenti ORF clone of Capn3 (Myc-DDK-tagged ORF) - Rat calpain 3 (Capn3), (10 ug) |
CNY 12,350.00 |
|
| RR211917L4 | Lenti ORF clone of Capn3 (mGFP-tagged ORF) - Rat calpain 3 (Capn3), (10 ug) |
CNY 13,490.00 |
