Tiprl (NM_001109667) Rat Tagged ORF Clone

CAT#: RR211522

  • TrueORF®

Tiprl (Myc-DDK-tagged ORF) - Rat TIP41, TOR signaling pathway regulator-like (S. cerevisiae) (Tiprl), (10 ug)



  "NM_001109667" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms RGD1310442
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR211522 representing NM_001109667
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGATCCACGGCTTTCAGAGCAGCCACCAGGACTTCTCCTTCGGGCCTTGGAAGCTTACCGCGTCCA
AGACCCACATCATGAAGTCTGCGGATGTGGAAAAGTTAGCCGACGAGCTGCATATGCCATCCCTCCCTGA
AATGATGTTTGGAGACAACGTTCTAAGGATCCAGCATGGTTCTGGCTTCGGAATAGAGTTCAATGCTACG
GATGCCCTGAGATGTGTGAACAACTACCAGGGCATGCTCAAAGTAGCCTGTGCTGAAGAGTGGCAGGAAA
GTAGGACGGAGGGTGAACACTCCAAAGAAGTTATTAAACCATATGACTGGACCTATACAACAGATTATAA
AGGAACGTTGCTTGGAGAATCTCTTAAGTTAAAGGTTGTACCTACAACAGATCATATAGATACAGAAAAA
TTGAAAGCCAGAGAGCAGATTAAATTTTTTGAAGAAGTTCTCCTGTTTGAAGATGAATTACATGATCATG
GCGTTTCCAGCCTGAGTGTGAAAATTAGAGTAATGCCTTCCAGCTTTTTTCTGCTATTGCGGTTTTTCCT
GAGAATTGATGGGGTGCTCATCAGAATGAATGACACAAGGCTTTATCATGAGGCTGACAAGACCTACATG
TTACGAGAATATACATCCAGAGAGAGCAAAATTGCTAATTTAATGCATGTTCCACCTTCCCTCTTCACGG
AACCTAATGAAATATCACAATATTTACCAATTAAGGAAGCAGTTTGTGAGAAGCTAGTATTTCCAGAAAG
AATTGATCCTAACCCCGTGGACTCAGAAAGTGCGCCCTCAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR211522 representing NM_001109667
Red=Cloning site Green=Tags(s)

MMIHGFQSSHQDFSFGPWKLTASKTHIMKSADVEKLADELHMPSLPEMMFGDNVLRIQHGSGFGIEFNAT
DALRCVNNYQGMLKVACAEEWQESRTEGEHSKEVIKPYDWTYTTDYKGTLLGESLKLKVVPTTDHIDTEK
LKAREQIKFFEEVLLFEDELHDHGVSSLSVKIRVMPSSFFLLLRFFLRIDGVLIRMNDTRLYHEADKTYM
LREYTSRESKIANLMHVPPSLFTEPNEISQYLPIKEAVCEKLVFPERIDPNPVDSESAPSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001109667
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001109667.1, NP_001103137.1
RefSeq Size 1726 bp
RefSeq ORF 816 bp
Locus ID 360869
UniProt ID A2VCX1
MW 31.2 kDa
Gene Summary May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. Acts as negative regulator of serine/threonine-protein phosphatase 4 probably by inhibiting the formation of the active PPP4C:PPP4R2 complex; the function is proposed to implicate it in DNA damage response by promoting H2AFX phosphorylated on Ser-140 (gamma-H2AFX). May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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