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Trpm8 (NM_134371) Rat Tagged ORF Clone
CAT＃: RR211342
- TrueORF®
Trpm8 (Myc-DDK-tagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10 ug)
"NM_134371" in other vectors (3)
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CNY 17,670.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211342 representing NM_134371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTCGAGGGAGCCAGGCTCAGCATGAGGAGCCGCAGAAATGGAACTCTGGGCAGCACCCGGACCC TGTACTCCAGCGTGTCTCGGAGCACAGACGTGTCCTACAGTGAAAGTGATTTGGTGAATTTTATTCAGGC AAATTTTAAAAAACGAGAATGCGTCTTCTTTACCAGAGACTCCAAGGCCATGGAGAGCATATGCAAGTGT GGTTATGCCCAGAGCCAGCATATCGAAGGCACCCAGATCAACCAAAATGAGAAGTGGAACTACAAAAAAC ACACCAAGGAGTTTCCAACAGACGCCTTTGGGGACATTCAGTTTGAGACTCTGGGGAAGAAAGGCAAGTA CTTACGCTTATCCTGTGACACGGACTCTGAAACCCTCTACGAACTGCTGACCCAGCACTGGCACCTCAAA ACACCCAACCTGGTCATCTCAGTGACGGGTGGAGCCAAAAACTTTGCTTTGAAGCCACGCATGCGCAAAA TCTTCAGTCGGCTGATCTACATCGCTCAGTCTAAAGGGGCATGGATTCTTACCGGAGGCACTCATTACGG TCTGATGAAGTACATAGGTGAAGTGGTGAGGGATAACACCATCAGCAGGAACTCGGAAGAGAACATCGTG GCCATTGGCATAGCGGCCTGGGGCATGGTCTCCAACAGGGACACCCTCATCAGGAATTGTGATGATGAGG GACATTTTTCAGCTCAATATATCATGGATGACTTCATGAGAGATCCTCTCTACATCCTGGACAACAATCA TACCCACCTGCTGCTTGTGGACAACGGTTGTCATGGACACCCCACGGTGGAAGCCAAACTTCGGAATCAG CTGGAGAAGTACATCTCTGAGCGCACCAGTCAAGATTCCAACTATGGTGGTAAGATCCCCATCGTGTGTT TTGCCCAGGGAGGTGGAAGAGAAACTTTGAAAGCCATCAACACCTCTGTCAAAAGTAAGATCCCCTGTGT GGTGGTGGAAGGCTCGGGGCAGATTGCCGATGTGATTGCCAGCCTGGTGGAGGTAGAGGATGTTTTAACC TCTTCCATGGTCAAAGAGAAGCTGGTACGGTTTTTACCCCGCACTGTGTCCCGGCTGCCTGAAGAGGAGA TTGAGAGCTGGATCAAATGGCTCAAAGAAATTCTTGAGAGCCCCCACCTCCTCACGGTCATCAAGATGGA GGAGGCTGGAGACGAGGTCGTGAGCAGCGCCATTTCCTACGCGCTGTACAAAGCCTTCAGCACTAATGAA CAAGACAAGGACAACTGGAACGGACAGCTGAAGCTTCTGCTGGAGTGGAACCAACTGGACCTTGCCAGTG ATGAGATCTTCACCAATGACCGCCGCTGGGAGTCTGCCGACCTTCAGGAAGTCATGTTCACGGCCCTCAT AAAGGACAGGCCCAAGTTTGTCCGCCTCTTCCTGGAGAATGGCCTCAACCTGCAGAAGTTCCTCACCAAT GAAGTCCTCACGGAGCTCTTCTCCACCCACTTCAGCACCCTAGTGTACCGGAACCTGCAGATCGCCAAGA ACTCCTACAACGATGCACTCCTTACCTTTGTCTGGAAGTTGGTGGCAAACTTCCGTAGAAGCTTCTGGAA AGAGGACAGAAGCAGCAGGGAGGACTTGGATGTGGAACTCCATGATGCATCTCTCACCACCCGGCACCCC CTGCAGGCTCTTTTCATCTGGGCCATTCTTCAGAACAAGAAGGAACTCTCCAAGGTCATCTGGGAGCAAA CCAAAGGCTGTACTCTGGCCGCCTTGGGGGCCAGCAAACTTCTGAAGACCCTGGCCAAAGTTAAGAATGA TATCAACGCAGCTGGGGAATCTGAGGAACTGGCTAATGAGTATGAGACCCGAGCAGTGGAGTTGTTCACT GAGTGTTACAGCAGTGATGAGGACTTGGCAGAACAGCTACTGGTCTACTCTTGTGAAGCCTGGGGTGGGA GCAACTGTCTGGAGCTGGCGGTGGAGGCTACGGACCAGCATTTCATTGCTCAGCCTGGGGTCCAGAATTT CCTTTCTAAGCAATGGTATGGAGAGATTTCCCGAGACACGAAGAACTGGAAGATTATCCTGTGTCTGTTC ATCATCCCCCTGGTGGGCTGTGGCCTCGTATCGTTTAGGAAGAAGCCCATTGACAAGCACAAGAAGCTGC TCTGGTACTACGTGGCCTTCTTCACTTCGCCCTTCGTGGTCTTCTCCTGGAACGTGGTCTTCTACATCGC CTTCCTCCTGCTGTTTGCGTATGTGCTGCTCATGGACTTCCACTCGGTGCCACACACCCCCGAGCTGATC CTCTATGCCCTGGTCTTCGTCCTCTTCTGTGATGAAGTGAGGCAGTGGTACATGAACGGAGTGAATTATT TCACCGACCTATGGAACGTTATGGACACACTGGGACTTTTCTACTTCATAGCGGGTATTGTATTCCGGCT TCACTCTTCAAATAAAAGCTCTTTGTACTCCGGGCGAGTCATTTTCTGTCTGGATTACATTATATTCACT CTAAGGCTCATCCACATTTTCACCGTGAGCAGGAACCTGGGACCCAAGATTATAATGCTGCAGCGGATGC TCATCGACGTTTTCTTCTTCTTGTTTCTCTTTGCTGTGTGGATGGTGGCCTTCGGCGTAGCCAGACAGGG GATCCTTAGGCAAAATGAACAGCGCTGGAGGTGGATCTTCCGCTCTGTCATCTATGAGCCCTACCTGGCC ATGTTTGGCCAGGTGCCCAGTGATGTGGACAGTACCACATATGACTTCTCCCACTGCACCTTCTCGGGAA ATGAGTCCAAGCCACTGTGCGTGGAGCTAGATGAATACAATCTGCCCCGCTTCCCTGAGTGGATCACCAT CCCACTAGTGTGCATCTACATGCTCTCCACCAACATCCTTCTGGTCAATCTCCTGGTCGCCATGTTTGGC TACACGGTGGGCATTGTGCAGGAGAACAACGATCAGGTCTGGAAGTTCCAGCGGTACTTCCTGGTGCAGG AGTACTGCAACCGCCTCAACATCCCCTTCCCCTTCGTCGTCTTCGCTTACTTCTACATGGTGGTCAAGAA GTGTTTCAAATGCTGCTGTAAAGAGAAGAACACGGAGTCTTCTGCCTGCTGTTTCAGAAATGAGGACAAC GAGACTTTGGCGTGGGAGGGCGTCATGAAGGAGAATTACCTTGTCAAGATCAACACGAAGGCCAACGACA ACGCAGAGGAGATGAGGCATCGGTTCAGACAACTGGACACAAAGCTTAATGATCTCAAAGGTCTTCTGAA AGAGATTGCTAATAAAATCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211342 representing NM_134371
Red=Cloning site Green=Tags(s) MSFEGARLSMRSRRNGTLGSTRTLYSSVSRSTDVSYSESDLVNFIQANFKKRECVFFTRDSKAMESICKC GYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFGDIQFETLGKKGKYLRLSCDTDSETLYELLTQHWHLK TPNLVISVTGGAKNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVVRDNTISRNSEENIV AIGIAAWGMVSNRDTLIRNCDDEGHFSAQYIMDDFMRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQ LEKYISERTSQDSNYGGKIPIVCFAQGGGRETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLT SSMVKEKLVRFLPRTVSRLPEEEIESWIKWLKEILESPHLLTVIKMEEAGDEVVSSAISYALYKAFSTNE QDKDNWNGQLKLLLEWNQLDLASDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLQKFLTN EVLTELFSTHFSTLVYRNLQIAKNSYNDALLTFVWKLVANFRRSFWKEDRSSREDLDVELHDASLTTRHP LQALFIWAILQNKKELSKVIWEQTKGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFT ECYSSDEDLAEQLLVYSCEAWGGSNCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLF IIPLVGCGLVSFRKKPIDKHKKLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHTPELI LYALVFVLFCDEVRQWYMNGVNYFTDLWNVMDTLGLFYFIAGIVFRLHSSNKSSLYSGRVIFCLDYIIFT LRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEQRWRWIFRSVIYEPYLA MFGQVPSDVDSTTYDFSHCTFSGNESKPLCVELDEYNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFG YTVGIVQENNDQVWKFQRYFLVQEYCNRLNIPFPFVVFAYFYMVVKKCFKCCCKEKNTESSACCFRNEDN ETLAWEGVMKENYLVKINTKANDNAEEMRHRFRQLDTKLNDLKGLLKEIANKIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134371 |
| ORF Size | 3312 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134371.2, NP_599198.2 |
| RefSeq Size | 4184 bp |
| RefSeq ORF | 3315 bp |
| Locus ID | 171384 |
| UniProt ID | Q8R455 |
| MW | 127.6 kDa |
| Gene Summary | excitatory ion channel; may be involved in transducing cold stimuli [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211342 | Trpm8 (untagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10 ug) |
CNY 11,544.00 |
|
| RR211342L3 | Lenti ORF clone of Trpm8 (Myc-DDK-tagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10 ug) |
CNY 19,290.00 |
|
| RR211342L4 | Lenti ORF clone of Trpm8 (mGFP-tagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10 ug) |
CNY 21,280.00 |
