Fyco1 (NM_001106870) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210985 representing NM_001106870 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTAGCAGCACTGAGACGCAGCTCCAGAGGATCATCCGTGATCTACAAGATGCAGTGACAGAAC TAAGCAATGAATTTAAGGAAGGCGGAGAACCAATCACAGATGACAGCACCAGCTTGCACAAGTTTTCTTA CAAACTTGAGTATCTTCTGCAATTTGATCAGAAGGAGAAGGCTAGCCTCCTGGGTAGCAAGAAGGACTAC TGGGATTACTTCTGTGCGTGCCTAGCCAAGGTGAAAGGAGCCAATGATGGGATTCGATTCGTCAGGTCCA TCTCTGAGCTCCGAACATCTCTAGGAAAGGGGAGAGCCTTCATTCGCTACTCTTTGGTGCACCAGAGGTT GGCAGACACCTTGCAGCAGTGCTTCATGAACACCAAAGTGACCAGTGACTGGTATTATGCAAGAAGCCCC TTTCTGAAGCCAAAGCTGAGCTCTGACATTGTGGGCCAGCTGTATGAGCTGACTGAGGTTCAGTTTGATC TAGCATCAAGAGGCTACGACTTGGATGCCGCCTGGCCAACATTTGCCAGGAGGACGCTAGCCACCAGCTC TTCTTACATGTGGAAACCCCCCAGCCGAAGCTCCAGCATGAGTAGTTTAGTGAGCAACTACTTGCAGACT CAGGAGATGGCCTCCAGTCTTGACCTGAATAGTTCTCTAAACAATGAAGCACTAGAAAGCTTCGATGAGA TGCGGCTGGAGCTGGACCAGTTGGAGGTTCGGGAGAAACAGCTACAAGAGCACGTGCAGCAGCTAAACAG GGAAAACCAAGCACTTCGAATGACGATCAGCAAGCAAGGGGGGCAACTTCAGGTAGAGAAGGAAACGGGG TACCTTGCAGTCGAGGATAGCATTGACCTCGTGAGCCTGGTGGCAGAGCTCCAGAAGCAGGGGGAGGTCA CCCAGGCCACAGTGAAGAAGCTACAGACATGTCTACAGGCTCTGGAGTTAGCTGTAGACCAGAAAGAGAC CAGCCACTCAGTCTTGCAGCTTGAGAACGTGGCACAGGCACTTGACGCTGTGAGAGACTCCTTGGGTAGG AAGAACCAGCTTCTAGCCAGCCTTTCTGAATGCCTCACTAGGGCTGAAAAAAGGGAACATCCATCTCTGG ACACAAAGCTTCATCAAGAGCCAGTTCCTACTGACCTGGCCCTGAAGTTCCAGGAGCTGAAGGGAAAACT TCAAACCCTAGAAGGAGAGAACACCAAGGCCCAGGAGCTAAACAGGCAGCAAAGTCTCAAGCTAGAGCAG CTGGCCAAGGAGCTGCAGCTGAAGGAGGAAGCCCGAGCTAGCCTGGAACACTTAGTGAAGGATGCGGTCC CACTTCAGGAAGAGCTATCCGGAAAGAAGCAAGAGTCAGCTCAGCTCCGACGACAGCTTCAGGAATCCCT GGCCCACTTGAGCTCTGTGGAGGAGGAGCTGGCTGAAGCCAGACAGCAGGAAAAGCAGCATCGAGAAGAA AAACAGCTACTAGAGCAGGAGGCCAAGTCTCTGACATGGCAGCTGCAGCTCCTAGAGACCCAACTAGGAC AGGTGAGTCAGCTTGTGAGTGACCTGGAGGAGCAGAAGAAGCAACTCATGCAGGACAAAGACCATCTCAG CCAGAAAGTAGGAACACTAGAGCAGCTAGCTGAGGCCCATGGGGCAAGTAAGAGTAAAGAGGCTGTCCCA CCACAGGCTGGGGAGATGCCAGAAAAGGGGCAGCACTGCCTCCAGGAAGAACAGGTAAACAATGCCACAG TGCGAGGGGCAGACCAGGACGAGTTACAAAAGGTCAACCAGGAGCTACAGAAGGAGCTGCAGAACATGGC TGTGCGCAACCAGCTCCTAGAAGGCAAACTGCAGGCCTTGCAAACTGATTACAAGGCACTGCAGCAGCGA GAGGCAGCAATCCAGGGCTCTTTAGCCTCCCTGGAAGCTGAGCAGGCCAGCATCCGGCAGCTGGGAAACC AGATGGAAGCAAGCCTCCTGGCTGTGAGGAAAGCCAAAGAGACTATGAAGGCCCAGGTGGCAGAGAAGGA GGCTGCCCTGCAGAGTAAGGAGGGCGAGTGCCGGCAGCTGCAGGAAGAGGCAGAGCAGTGCCGGCTTCGG ACAGAGGCCCAGGCCCAGGAGCTCAGAGCTCTTGAGAACCAGTGCCAGCAGCAGATTCAACTGATTGAGG TCCTCTCTGCAGAAAAAGGCCAACAGGGACTCAGCCTACCCCAGGTCAACACAGCCCAACTGGCCCTGTC TCAAGCACAGCTTGAAATTCACCAGGGGGAAGCCCAGCGGTTACAGAATGAGGTGGTGGACCTCCAGGCC AAGCTCCAGGTGGCCCTAGGTGATCGAGACAAGTTACAGAGCCAACTGGGTGTGGCTGAGGCAGTTCTCG GGGAGCACAAGACCCTGGTGCAACAACTGAAAGAACAGAATGAAGCCCTCAACAGGGCCCATATTCAGGA GCTGCTGCAGTGCTCAGAGAGAGAGGGGATACTGCAGGAGGAGAGCATCTACAAGGCCCAGAAGCAGGAG CAAGAGCTACGAGCCCTGCAGGCAGAGCTGTCACAGGTGAGGTGCAGCTCTGAGGGAGCCCACCTGGAGC ATGCAGAGCTACAGGACCAGCTGCACCGAGCCAACACAGACACAGCTGAGCTAGGCATACAGGTCTGTGC ACTGACAGCTGAGAAGGATCGGATGGAGGGGGCCCTGGCCAGCCTAGCCCAGGAGCTCCAGGACTCCAAA GAAGCAGCACTACAGGAACGAAAGGGCTTAGAGCTCCAAGTGATGCAACTTCAGCAAGAGAAGGAGAGCT TGCAGGAAAAGCTGAAGGCAACTGAGGAGGCAGCCAGTTCATTCTCTGTTCTTCAGGCACAGCTGGCCCA GGCTGAGCAGCTGGCCCAGAGCCTCCAAGAGGCTGCACACCAGGAACAAGATGCCCTTAAGTTCCAGCTA AGTGCTGAGATTATGGACCACCAGAACAGACTAAAGACAGCCAATGAAGAGTGTGGGAGCCTCAGGGCCC AGCTAGAAGAACAAGGCCAGGAGCTGCAAATGACTAAGGAGGCTGTGCAGGAACTGAAGATCACCAAGGC GACCATGGAGGAGAAGCTGAATTGCACCGGTAGCCACCTTGCAGAGTGCCAGGCCACTTTGCTACGCAAA GATGAAGAAAGCGCTATGCTTCGAATCAACCTAGAGAGAACACAGAAGGAACTTGAAAAGGCTACATCAA AATTCCAAGAATATTACAACAAACTCTGCCAGGAGGTGACAAACAGGGAAAGGAATGACCAGAAGATGCT TGCAGACCTAGATGACCTGAACAGAACCAAGAAATACCTTGAGGAGCGGCTGATAGAGCTGCTCAGGGAC AAAGATGCTCTCTGGCAAAAGTCCGATGCACTGGAATTCCAGCAGAAGCTCAGTGCTGAAGAGAAATGTC TTGGGGACATGGAGGCCAACCACTGCCATGACTGCAAGCGGGAGTTCAGCTGGATAGTGCGGCGGCACCA CTGCAGGTTATGTGGCCGAATATTCTGTTACTACTGCTGTAACAACTATGTTGTGACTAAGCCCAGTGGC AAGAAGGAACGGTGCTGCCGAGCCTGCTTCCAGAAGTTTGGTGAAAGCTCTGGATCCCCTGACAGCAGTG GTTCGGGCACTAGCCAGGGAGAGCCCAGCCCCATGGTGTCACCAGCTGAAGCAGCTCCCCAGTCCATAGG AAGCCAAGGAATAAACCCAGTCTGCAGACCACCAGATGATGCTGTGTTTGACATCATCACCGATGAGGAA CTGTGCCAGATCCAAGAATCTGGCTCCTCCTTGCCTGAAACACCCACCGAAACTGATTCGATGGACCCAA ATATGGCTGAACAGGACACCACATCAAACTCATTAACCCCTGAAGACACTGAAGACATGCCCCTGGGGCA GGATGCTGAAATCTGCTTGCTGAAGTCAGGAGAGCTGATGATCAAATTACCCCTCACAGTAGAGGAGATC GCCAGCTTTGGGGAGGGAAACAGGGAGCTGTTTGTGAGGTCCAGTACCTACAGCCTGATCCCCATCACCG TGGCTGAGCCCGGCCTTACCATCAGCTGGGTCTTCTCTTCTGATCCCAAGAGCATCTCCTTCAGTGTGGT CTTCCAAGAGACAGAAGACACGCCACTCGACCAGTGCAAGGTCCTCATTCCCACCACACGCTGCAATTCC CACAAGGAGAGCATCCGGGGCCAGCTGAAGGTCCGAACCCCTGGCATCTACTTGCTCATCTTTGACAACA CCTTTTCCAGATTTATCTCCAAAAAAGTATTTTACCACCTGACCGTTGACCGGCCTGTGATCTACGATGG AAGTGATTTCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210985 representing NM_001106870 Red=Cloning site Green=Tags(s) MASSSTETQLQRIIRDLQDAVTELSNEFKEGGEPITDDSTSLHKFSYKLEYLLQFDQKEKASLLGSKKDY WDYFCACLAKVKGANDGIRFVRSISELRTSLGKGRAFIRYSLVHQRLADTLQQCFMNTKVTSDWYYARSP FLKPKLSSDIVGQLYELTEVQFDLASRGYDLDAAWPTFARRTLATSSSYMWKPPSRSSSMSSLVSNYLQT QEMASSLDLNSSLNNEALESFDEMRLELDQLEVREKQLQEHVQQLNRENQALRMTISKQGGQLQVEKETG YLAVEDSIDLVSLVAELQKQGEVTQATVKKLQTCLQALELAVDQKETSHSVLQLENVAQALDAVRDSLGR KNQLLASLSECLTRAEKREHPSLDTKLHQEPVPTDLALKFQELKGKLQTLEGENTKAQELNRQQSLKLEQ LAKELQLKEEARASLEHLVKDAVPLQEELSGKKQESAQLRRQLQESLAHLSSVEEELAEARQQEKQHREE KQLLEQEAKSLTWQLQLLETQLGQVSQLVSDLEEQKKQLMQDKDHLSQKVGTLEQLAEAHGASKSKEAVP PQAGEMPEKGQHCLQEEQVNNATVRGADQDELQKVNQELQKELQNMAVRNQLLEGKLQALQTDYKALQQR EAAIQGSLASLEAEQASIRQLGNQMEASLLAVRKAKETMKAQVAEKEAALQSKEGECRQLQEEAEQCRLR TEAQAQELRALENQCQQQIQLIEVLSAEKGQQGLSLPQVNTAQLALSQAQLEIHQGEAQRLQNEVVDLQA KLQVALGDRDKLQSQLGVAEAVLGEHKTLVQQLKEQNEALNRAHIQELLQCSEREGILQEESIYKAQKQE QELRALQAELSQVRCSSEGAHLEHAELQDQLHRANTDTAELGIQVCALTAEKDRMEGALASLAQELQDSK EAALQERKGLELQVMQLQQEKESLQEKLKATEEAASSFSVLQAQLAQAEQLAQSLQEAAHQEQDALKFQL SAEIMDHQNRLKTANEECGSLRAQLEEQGQELQMTKEAVQELKITKATMEEKLNCTGSHLAECQATLLRK DEESAMLRINLERTQKELEKATSKFQEYYNKLCQEVTNRERNDQKMLADLDDLNRTKKYLEERLIELLRD KDALWQKSDALEFQQKLSAEEKCLGDMEANHCHDCKREFSWIVRRHHCRLCGRIFCYYCCNNYVVTKPSG KKERCCRACFQKFGESSGSPDSSGSGTSQGEPSPMVSPAEAAPQSIGSQGINPVCRPPDDAVFDIITDEE LCQIQESGSSLPETPTETDSMDPNMAEQDTTSNSLTPEDTEDMPLGQDAEICLLKSGELMIKLPLTVEEI ASFGEGNRELFVRSSTYSLIPITVAEPGLTISWVFSSDPKSISFSVVFQETEDTPLDQCKVLIPTTRCNS HKESIRGQLKVRTPGIYLLIFDNTFSRFISKKVFYHLTVDRPVIYDGSDFP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106870
ORF Size	4353 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106870.1, NP_001100340.1
RefSeq Size	7743 bp
RefSeq ORF	4356 bp
Locus ID	301085
MW	163.6 kDa

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