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Nudt21 (NM_001039004) Rat Tagged ORF Clone
CAT＃: RR210891
- TrueORF®
Nudt21 (Myc-DDK-tagged ORF) - Rat cleavage and polyadenylation specific factor 5 (Cpsf5), (10 ug)
"NM_001039004" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cpsf5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210891 representing NM_001039004
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTGGTGCCGCCCAATCGCTCGCAGACGGGCTGGCCCCGGGGGGTCAACCAATTCGGTAACAAGT ACATCCAGCAAACCAAGCCCCTCACCCTGGAGCGCACCATTAACCTGTACCCACTTACCAACTATACTTT TGGTACAAAGGAGCCCCTCTATGAAAAGGACAGCTCTGTGGCGGCCAGATTTCAGCGCATGCGGGAGGAA TTTGATAAGATTGGGATGAGAAGGACTGTAGAAGGGGTTCTGATCGTACACGAGCACCGCCTGCCCCACG TGCTACTGCTACAGCTGGGAACAACCTTCTTCAAACTACCTGGCGGTGAACTTAACCCAGGAGAAGATGA AGTTGAAGGACTAAAACGCTTAATGACAGAGATACTGGGTCGTCAAGATGGAGTCCTGCAAGACTGGGTC ATCGATGACTGCATTGGGAACTGGTGGAGACCAAATTTTGAACCTCCTCAGTACCCGTATATTCCTGCAC ATATAACAAAGCCCAAGGAACATAAGAAGTTGTTTCTGGTTCAGCTTCAAGAGAAAGCCTTGTTTGCGGT CCCTAAGAATTACAAGCTGGTAGCTGCACCGTTGTTTGAACTGTACGACAATGCTCCGGGATACGGACCC ATCATTTCTAGTCTTCCTCAGCTGCTGAGCAGGTTTAATTTTATATACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210891 representing NM_001039004
Red=Cloning site Green=Tags(s) MSVVPPNRSQTGWPRGVNQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREE FDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWV IDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGP IISSLPQLLSRFNFIYN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039004 |
| ORF Size | 681 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039004.1, NP_001034093.1 |
| RefSeq Size | 1074 bp |
| RefSeq ORF | 684 bp |
| Locus ID | 291877 |
| UniProt ID | Q4KM65 |
| MW | 26.2 kDa |
| Gene Summary | Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210891 | Nudt21 (untagged ORF) - Rat cleavage and polyadenylation specific factor 5 (Cpsf5), (10 ug) |
CNY 3,990.00 |
|
| RR210891L3 | Lenti ORF clone of Nudt21 (Myc-DDK-tagged ORF) - Rat cleavage and polyadenylation specific factor 5 (Cpsf5), (10 ug) |
CNY 6,080.00 |
|
| RR210891L4 | Lenti ORF clone of Nudt21 (mGFP-tagged ORF) - Rat cleavage and polyadenylation specific factor 5 (Cpsf5), (10 ug) |
CNY 6,650.00 |
