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Ube4a (NM_207610) Rat Tagged ORF Clone
CAT＃: RR210715
- TrueORF®
Ube4a (Myc-DDK-tagged ORF) - Rat ubiquitination factor E4A (Ube4a), (10 ug)
"NM_207610" in other vectors (3)
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CNY 17,100.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ab2-232 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210715 representing NM_207610
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTG ATGCCAAGCAGTTTGCAGCAATCCACAAAGAGCAGCTGAAACAGCAGTCTGATGAGCTCCCAGCAAGCCC AGATGACTCAGATAATAGTGTGTCGGAGAGCCTGGATGAGTTTGACTACTCAGTGTCTGAGATCAGCCGC TCTTTCCGGACACATCAAGAAATGTGTGAACAACTCAACATCAATCACATGATCCAAAGGATCTTCCTCA TTACTCTGGACAACAGTGATCCAAACTTGAAAAGTGGGAACGGCATCCCCAGTCGGTGCGTGTACTTGGA AGAAATGGCAGTGGAGCTGGAAGATCAAGACTGGCTGGATATGAGCAACGTGGAGCAGGCCATCTTTGCT CGGTTACTGCTTCAAGATCCAGGAAACCACTTGATCAGCATGACTTCCTCTGCAACTTTAAACCTCTCTG CTGATCGCGATGCAGGAGAGCGGCACATCTTCTGTTACCTTTACTCCTGCTTCCAAAGAGCCAAGGAAGA GATTACCAAAGTTCCAGAAAACCTACTCCCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAACACG CGAACAGTTCTCCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGACTTGATGT TGGAAGCTATTCAGGGAGCCCATTTTGAAGATGTGACTGAGTTTTTAGAAGAGGTCATTGAAGCCTTGCT ATTGGATGAGGAGGTTCGAACATTTCCTGAAGTCATGATTCCAGTGTTTGATATTTTACTTGGCCGAATA AAAGATCTGGAACTCTGCCAGATCCTCCTGTACGCATACCTGGACATCCTTCTCTATTTCACCAGGCAGA AGGATATGGCCAAGGTTTTTTTAGAGTACATTCAGCCCAAGGACCCTAGCAATGGGCAGATGTATCAGAA GACCTTGCTGGGAGTCATTCTGAATATCTCGTGCTTGTTAAAGACTCCGGGTGTGGTAGAGAATCATGGC TTCTTCCTGAATCCATCCCGTTCCAGTCCTCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCA TGGCTCAGTTCCATGAAAAGATCTACCAGATGCTGAAGAATCTGCTCCAGCTCTCTCCAGAAACCAAGCA TGGTATCTTGTTCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATCTGGGCCAATCAG ATGCCCGAAATCTTCTTCCAAATGTATGCCTCGGATGCCTTCTTTCTGAATCTGGGTGCTGCTCTCCTGA AGCTGTGCCAGCCATTCTGCAAACCCAGATCCTCTCGGCTCCTCACCTTTAACCCCACATACTGTGTCCT CAAGGACTTGAATGATGAAGAACGAAAAATTAAAAGTGTACATATGAGAGGTTTAGACAAGGAGACCTGT CTGATCCCAGCTGTGCAGGAGCCAGTGTTCCCCCAGAGCTACAATCTGGTGACCGAGAACCTGGCCCTGA CCGAGTATACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCA TCGGCTGCAGGTTGCCTGGCGAGATGCTCAGCAGAGTTCTAGCCCTGCTGCTGACAATCTCCGTGAGCAG TTCGAGCGCCTGATGACCATCTATCTCTCTACCAAGACTGCAATGACTGAACCCCAGATGCTGCAGAACT GCCTGAATTTGCAGGTGTCCATGGCTGTTCTGCTGGTTCAACTGGCCATAGGCAATGAGGGCTCTCAGCC AATAGAGCTAAGTTTTCCCCTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGAT AACCTAGGTGATTTCCTCATTTTTCTCCGGCGCTTTGCTGAGGACATTTTGGAGACGTCAGCAGATTCCC TGGAGCATGTCCTTCATTTTATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGAG GGCCAAACTAGCAGAGGTGTTAGAAGCAGTGATGCCTCACCTGGACCAGACCCCAAGCCCCTTGGTGTCC AGTGTGTTCCACCGAAAACGTGTGTTCTGCAACTTTCCATACGCTCCCCAACTTTCTGAAGCCTTGATCA AGGTTTTTGTGGACATCGAATTTACAGGGGACCCCCACCAATTTGAACAGAAGTTTAATTACCGCCGCCC TATGTATCCCATCCTAAGATACATGTGGGGGACAGATAGCTATCGGGAGAGCATTAAGGATTTGGCTGAC TATGCCTCTAAGAATTTAGAAGCTATGAATCCCCCACTGTTCCTCCGATTTCTTAACCTATTAATGAATG ATGCTATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAGATTGAGAAAGA CCGAGGAGAATGGGAGAGCCTGACGCCAGAAGCCCGCCGAGAGAAAGAGGCAGGTCTGCAGATGTTTGGG CAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATTGGGACCCTTTCCTTCCTGACATCAGAGA TCAAGTCACTGTTCGTGCACCCGTTCCTGGCCGAGCGCATCATCTCCATGCTCAACTACTTCCTGCAGCA CCTGGTTGGCCCCAAGATGGGTGCTCTCAAAGTCAAGGACTTCAGCGAATTTGATTTCAAACCTCAGCAG CTTGTATCAGACATCTGCACTATCTACTTAAATCTTGGAGATGAGGAGAATTTCTGTGCCACTGTGCCCA AGGACGGACGTTCCTATTCCCCTACTCTCTTTGCACAGACAGTCCGAGTCCTGAAGAAAATCAATAAGCC CGGGAATATGATCGTGGCTTTCAGTAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAA GAGGAGGAGACATATGCAGATGCCTGCGATGAGTTCCTGGACCCCATCATGAGCACACTGATGTCTGACC CTGTGGTGCTTCCGTCTTCCAGAGTCACTGTGGACGGATCCACCATCGCCAGGCATTTACTCAGTGACCA AACAGATCCTTTTAATCGTAGTCCCCTTACCATGGACCAGATCCGGCCAAACACAGAACTGAAAGAAAAA ATTCAACGGTGGCTTGCAGAGAGGAAGCAACAAAAGGAGCAGCTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210715 representing NM_207610
Red=Cloning site Green=Tags(s) MTDQENNNNISSNPFAALFGSLADAKQFAAIHKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVSEISR SFRTHQEMCEQLNINHMIQRIFLITLDNSDPNLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQAIFA RLLLQDPGNHLISMTSSATLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNT RTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALLLDEEVRTFPEVMIPVFDILLGRI KDLELCQILLYAYLDILLYFTRQKDMAKVFLEYIQPKDPSNGQMYQKTLLGVILNISCLLKTPGVVENHG FFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHGILFWLGNCLHANAGRTKIWANQ MPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCVLKDLNDEERKIKSVHMRGLDKETC LIPAVQEPVFPQSYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQ FERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELSFPLPDGYSSLAYVPEFFAD NLGDFLIFLRRFAEDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPSPLVS SVFHRKRVFCNFPYAPQLSEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRESIKDLAD YASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWESLTPEARREKEAGLQMFG QLARFHNIMSNETIGTLSFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQ LVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVAFSNLAERIKSLADLQQQ EEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDGSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEK IQRWLAERKQQKEQLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_207610 |
| ORF Size | 3198 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_207610.1, NP_997493.1 |
| RefSeq Size | 6041 bp |
| RefSeq ORF | 3201 bp |
| Locus ID | 315608 |
| UniProt ID | Q6P7A2 |
| MW | 122.4 kDa |
| Gene Summary | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210715 | Ube4a (untagged ORF) - Rat ubiquitination factor E4A (Ube4a), (10 ug) |
CNY 17,100.00 |
|
| RR210715L3 | Lenti ORF clone of Ube4a (Myc-DDK-tagged ORF) - Rat ubiquitination factor E4A (Ube4a), (10 ug) |
CNY 18,720.00 |
|
| RR210715L4 | Lenti ORF clone of Ube4a (mGFP-tagged ORF) - Rat ubiquitination factor E4A (Ube4a), (10 ug) |
CNY 20,520.00 |
