Arhgef11 (NM_023982) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gtrap48
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210628 representing NM_023982 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATACGATTGCCCCATAGTATAGACAGATCAGCCAGTAAAAAGCAGTCTCACCTGTCCAGTCCCA TTGCATCCTGGTTAAGTAGCCTGTCTTCTCTGGGAGATTCTACACCTGAACGCACATCCCCTTCTCACCA CCGCCAGCCCTCTGACACTTCTGAGACAACAGCAGGTCTTGTTCAGCGCTGTGTCATCATCCAAAAGGAC CAGCATGGCTTTGGCTTCACAGTTAGTGGAGATCGCATTGTACTGGTGCAGTCCGTGCGCCCTGGAGGCG CAGCCATGAAAGCTGGTGTGAAAGAGGGTGACCGGATCATCAAAGTAAACGGCACCATGGTGACCAATAG CTCACACCTGGAGGTGGTAAAGCTTATCAAATCTGGCGCCTATGCTGCGCTTACCCTCCTAGGCTCTTCT CCTCCCTCCGTCGGCGTCTCTGGGCTCCAGCAGAATCCATCTGTGGCAGGAGTGCTCAGAGTTAACCCCA TCATTCCTCCACCACCTCCCCCGCCACCCTTGCCACCTCCACAGCACATTACTGGACCCAAACCTCTTCA GGATCCTGAAGTCCAAAAGCACGCCACTCAAATCCTCTGGAATATGCTAAGACAGGAGGAGGAAGAGTTA CAGGACATACTTCCACCCTGTGGTGAGACCAGTCAGAGAACATGTGAGGGCCGCCTCTCTGTGGACTCCC AGGAGGCAGACAGTGGCTTGGATTCTGGGACAGAACGCTTTCCCTCCATCAGTGAGTCATTGATGAATCG GAACTCAGTATTGTCAGATCCTGGACTAGACAGCCCTCAAACCTCCCCTGTAATCCTGGCCAGGGTGGCC CAGCACCACAGGCGACAGGGCTCAGATGCAGCGTTGCTCCCGCTCAACCACCAGGGTATAGATCAAAGCC CAAAGCCTCTGATTATTGGCCCAGAGGAAGATTATGACCCAGGTTATTTCAACAATGAGAGTGACATCAT CTTCCAAGATCTTGAAAAACTGAAGTCACATCCAGCTTACTTGGTAGTTTTTCTACGTTACATCCTCTCT CAGGCAGACCCTGGCCCCCTGCTTTTTTATTTGTGTTCAGAAGTTTATCAACAGACAAATCCCAAAGATT CCCGAAGTCTGGGGAAAGACATCTGGAACATTTTCCTGGAGAAAAATGCGCCTCTCAGAGTGAAGATCCC TGAGATGTTGCAGGCTGAAATTGACCTACGCCTGCGGAACAATGAGGACCCTCGCAATGTGCTCTGTGAA GCTCAGGAGGCAGTCATGCTGGAAATCCAGGAGCAGATCAACGACTACAGATCCAAGCGTACTCTGGGCC TGGGCAGCCTCTATGGTGAAAATGACCTGCTAGGCCTGGATGGGGACCCTCTTCGAGAACGCCAAATGGC TGAGAAGCAGCTGGCTGCCCTTGGAGATATCTTGTCCAAATATGAGGAAGATCGGAGTGCCCCCATGGAC TTTGCTGTTAATACCTTTATGAGCCACGCTGGGATCCGTCTTCGGGAGTCTCGATCCTCCTGCACGGCAG AAAAGACCCAGTCTGCCCCTGACAAGGACAAGTGGCTGCCCTTCTTCCCTAAGACCAAGAAGCAGAGCAG CAATTCCAAGAAAGAAAAGGATGCCTTGGAGGACAAGAAGCGAAACCCCATCCTCAGATATATTGGGAAG CCCAAGAGCTCCTCTCAGAGCATTAAGCCAGGCAATGTGAGGAACATCATTCAGCACTTTGAGAACAGCC ATCAGTATGATGTCCCAGAGCCGGGGACACAACGACTCTCAACAGGAAGCTTTCCTGAGGACCTGCTGGA GAGTGACAGTTCGCGCTCAGAGATTCGACTGGGCCGCTCTGGGAGCCTCAAGGGCCGGGAAGAGATGAAG CGATCCCGGAAAGCAGAGAACGTGCCCCGGCCTCGAAGTGACGTTGACATGGATGCTGCTGCAGAGGCTG CCCGCCTTCACCAGTCAGCCTCGTCCTCTGCCTCCAGCCTCTCCACCAGGTCTCTTGAGAACCCAACCCC TCCCTTCACCCCCAAAATGGGCCGCAGGAGCATTGAGTCCCCCAATCTGGGGTTCTGTACAGACGTCATC CTTCCCCACCTCCTGGAGGATGATCTGGGCCAATTGTCTGACCTGGAGCCAGAGCCAGAGGTCCAAAACT GGCAGCATACAGTAGGCAAGGATGTGGTGGCCAACCTGACCCAGAGGGAAATTGACCGGCAAGAGGTCAT CAATGAGCTTTTTGTGACAGAAGCATCTCACCTGCGCACACTCCGAGTCCTGGACCTCATCTTCTACCAG CGCATGAGAAAGGAGAACCTAATGCCTCGGGAAGAGCTAGCGCGGCTCTTCCCTAACCTGCCTGAGCTCA TAGAGATTCACAATTCCTGGTGTGAGGCCATGAAGAAGCTCCGGGAGGAGGGCCCCATTATCAGAGACAT CAGTGACCCCATGCTGGCTCGGTTTGATGGTCCTGCCCGAGAAGAACTCCAGCAAGTAGCTGCACAATTC TGTTCCTATCAGTCAGTAGCCCTAGAGCTAATCAGGACTAAGCAACGTAAGGAGAGTCGGTTCCAGCTCT TCATGCAGGAGGCTGAGAGCCACCCTCAGTGCCGGCGTCTGCAGCTCCGAGACCTCATCGTCTCTGAAAT GCAACGGCTCACCAAGTACCCACTGCTGCTAGAGAACATCATCAAGCACACAGAGGGTGGCACCTCTGAG CATGAGAAGCTCTGCCGTGCCCGGGACCAGTGCCGGGAGATTCTCAAGTTTGTGAATGAAGCAGTAAAGC AGACAGAGAACCGCCACCGGCTAGAGGGGTACCAGAAACGCCTGGATGCCACTGCCCTAGAGCGGGCCAG CAACCCCTTGGCAGCAGAGTTCAAGAGCCTGGATCTTACAACAAGGAAGATGATCCACGAGGGGCCTCTG ACCTGGAGGATCAGCAAAGACAAGACCCTGGACCTCCAGGTGCTTCTGCTTGAGGACCTGGTGGTACTGC TGCAGAGACAAGAGGAGCGGCTGCTGCTAAAGTGCCACAGCAAGACAGCCGTGGGCTCCTCCGACAGCAA GCAGACGTTCAGCCCTGTGCTGAAGCTCAATGCTGTGCTCATCCGCTCCGTGGCTACAGACAAGCGAGCC TTCTTCATCATCTGCACCTCCGAGCTGGGCCCTCCCCAGATCTATGAGCTGGTTGCATTGACGTCATCAG ACAAGAATATATGGATGGAGCTCTTAGAAGAGGCCGTGCAGAATGCCACCAAGCACCCTGGAGCTGCCCC AATCCCCATCCATCCCTCACCACCAGGATCCCAGGAGCCGGCCTACCAGGGCTCCACCTCCAGCAGGGTA GAAATAAATGACTCAGAAGTATATCACACTGAAAAAGAACCCAAGAAGCTACCTGAAGGCCCCGGGCCTG AGCAGAGAGTTCAAGACAAGCAGCTGATAGCACAAGGGGAGCCTGTGCAGGAAGAGGATGAAGAGGAATT GAGGACCTTGCCTCGAGCTCCCCCCTCCCTGGATGGAGAAAACAGAGGCATCAGGACAAGGGACCCTGTC CTTCTGGCCCTCACAGGCCCTCTGCTCATGGAGGGACTTGCTGATGCTGCCCTGGAAGATGTGGAGAACT TGCGTCACCTGATCCTGTGGAGCCTGCTGCCTGGTCACACTGTGAAGACTCAGGCTGCTGGCGAGCCTGA GGATGACCTCACACCCACCCCTTCTGTCGTGAGCATCACCTCTCACCCCTGGGACCCAGGGTCCCCAGGG CAAGCTCCCACCATAAGTGACAGCACCCGACTTGCGAGGCCAGAGGGCAGCCAGCCAGAGGGCGAGGATG TTGCTGTCAGTTCTCTGGCACACCTGCCGCCAAGGACCAGAAGTTCTGGCGTCTGGGACTCTCCTGAGCT GGATAGGAATCCGGCTGCAGAGGCTGCAAGCACAGAACCAGCAGCAAGTTACAAGGTTGTGAGAAAAGTC TCTCTACTCCCTGGTGGTGGTGTCGGTGCAGCCAAGGTGGCGGGCAGCAATGCTATCCCTGACAGTGGCC AGTCAGAATCTGAGCTATCTGAAGTGGAAGGCGGAGCACAGGCTACGGGGAACTGTTTCTATGTCAGCAT GCCAGCAGGACCTCTGGACTCCAGCACTGAGCCTACTGGGACACCCCCAAGCCCCTCACAGTGTCACAGC CTCCCTGCATGGCCAACAGAGCCTCAGCCCTACAGGGGAGTCCGTGGGGGTCAGTGTTCCAGCCTGGTCC GCAGGGATGTGGATGTGATCTTCCATACCATCGAGCAGCTCACCATCAAGCTTCACAGACTGAAGGACAT GGAGCTGGCCCACAGAGAGCTGCTCAAGTCCCTTGGAGGAGAGTCATCGGGTGGAACCACACCTGTGGGG AGTTTTCACACAGAGGCAGCCAGATGGACAGACTACTCCCTCTCTCCTCCAGCCAAGGAAGCCCTGGCCT CTGATTCCCAAAATGGTCAGGAGCAGGGGTCCTGCCCTGAAGAAGGCTCCGACATCGCCCTGGAAGACAG TGCCACTGACACAGCTGTGTCACCAGGACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210628 representing NM_023982 Red=Cloning site Green=Tags(s) MSIRLPHSIDRSASKKQSHLSSPIASWLSSLSSLGDSTPERTSPSHHRQPSDTSETTAGLVQRCVIIQKD QHGFGFTVSGDRIVLVQSVRPGGAAMKAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYAALTLLGSS PPSVGVSGLQQNPSVAGVLRVNPIIPPPPPPPPLPPPQHITGPKPLQDPEVQKHATQILWNMLRQEEEEL QDILPPCGETSQRTCEGRLSVDSQEADSGLDSGTERFPSISESLMNRNSVLSDPGLDSPQTSPVILARVA QHHRRQGSDAALLPLNHQGIDQSPKPLIIGPEEDYDPGYFNNESDIIFQDLEKLKSHPAYLVVFLRYILS QADPGPLLFYLCSEVYQQTNPKDSRSLGKDIWNIFLEKNAPLRVKIPEMLQAEIDLRLRNNEDPRNVLCE AQEAVMLEIQEQINDYRSKRTLGLGSLYGENDLLGLDGDPLRERQMAEKQLAALGDILSKYEEDRSAPMD FAVNTFMSHAGIRLRESRSSCTAEKTQSAPDKDKWLPFFPKTKKQSSNSKKEKDALEDKKRNPILRYIGK PKSSSQSIKPGNVRNIIQHFENSHQYDVPEPGTQRLSTGSFPEDLLESDSSRSEIRLGRSGSLKGREEMK RSRKAENVPRPRSDVDMDAAAEAARLHQSASSSASSLSTRSLENPTPPFTPKMGRRSIESPNLGFCTDVI LPHLLEDDLGQLSDLEPEPEVQNWQHTVGKDVVANLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQ RMRKENLMPREELARLFPNLPELIEIHNSWCEAMKKLREEGPIIRDISDPMLARFDGPAREELQQVAAQF CSYQSVALELIRTKQRKESRFQLFMQEAESHPQCRRLQLRDLIVSEMQRLTKYPLLLENIIKHTEGGTSE HEKLCRARDQCREILKFVNEAVKQTENRHRLEGYQKRLDATALERASNPLAAEFKSLDLTTRKMIHEGPL TWRISKDKTLDLQVLLLEDLVVLLQRQEERLLLKCHSKTAVGSSDSKQTFSPVLKLNAVLIRSVATDKRA FFIICTSELGPPQIYELVALTSSDKNIWMELLEEAVQNATKHPGAAPIPIHPSPPGSQEPAYQGSTSSRV EINDSEVYHTEKEPKKLPEGPGPEQRVQDKQLIAQGEPVQEEDEEELRTLPRAPPSLDGENRGIRTRDPV LLALTGPLLMEGLADAALEDVENLRHLILWSLLPGHTVKTQAAGEPEDDLTPTPSVVSITSHPWDPGSPG QAPTISDSTRLARPEGSQPEGEDVAVSSLAHLPPRTRSSGVWDSPELDRNPAAEAASTEPAASYKVVRKV SLLPGGGVGAAKVAGSNAIPDSGQSESELSEVEGGAQATGNCFYVSMPAGPLDSSTEPTGTPPSPSQCHS LPAWPTEPQPYRGVRGGQCSSLVRRDVDVIFHTIEQLTIKLHRLKDMELAHRELLKSLGGESSGGTTPVG SFHTEAARWTDYSLSPPAKEALASDSQNGQEQGSCPEEGSDIALEDSATDTAVSPGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_023982
ORF Size	4581 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_023982.1, NP_076472.1
RefSeq Size	4584 bp
RefSeq ORF	4584 bp
Locus ID	78966
UniProt ID	Q9ES67
MW	168.5 kDa
Gene Summary	binds the intracellular carboxy-terminal domain of cerebellar glutamate transporter EAAT4; may modulate glutamate transporter activity [RGD, Feb 2006]

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