Plcb1 (NM_001077641) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Phosphb; PLC'1; PLCbeta1; RATPHOSPHB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210622 representing NM_001077641 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGCTCAGCCCGGAGTGCACGCCTTGCAACTCAAGCCCGTGTGCGTGTCCGACAGCCTCAAGA AGGGCACCAAATTCGTCAAGTGGGATGATGACTCCACTATAGTTACTCCAATTATTTTGAGGACCGATCC TCAGGGATTTTTCTTTTACTGGACAGATCAGAATAAGGAGACGGAGCTGTTAGATCTCAGCCTCGTCAAG GATGCCAGGTGTGGGAAGCACGCCAAAGCTCCCAAGGACCCCAAGTTACGTGAACTTCTGGATGTTGGGA ACATCGGACACTTGGAACAGCGCATGATAACTGTGGTGTATGGGCCAGACCTGGTGAATATCTCCCACCT GAATCTTGTGGCTTTTCAAGAGGAAGTGGCCAAGGAATGGACAAATGAGGTTTTCAGTTTGGCAACAAAC CTGCTGGCTCAGAACATGTCCAGGGACGCATTTCTGGAGAAAGCATATACTAAGCTCAAGCTTCAGGTGA CCCCAGAAGGGCGCATTCCTCTTAAAAACATCTATCGACTGTTTTCGGCAGACCGGAAGCGAGTGGAAAC TGCGCTAGAGGCTTGTAGTCTTCCATCGTCAAGGAACGATTCCATCCCTCAAGAGGACTTTACTCCAGAT GTATACAGAGTTTTCCTGAACAATCTTTGTCCCCGACCTGAAATTGATAACATCTTCTCTGAATTTGGTG CCAAAAGCAAACCGTACCTTACTGTTGATCAGATGATGGATTTTATCAACCTTAAGCAGAGAGATCCCCG GCTGAATGAAATACTTTACCCACCTCTGAAGCAAGAGCAGGTCCAAGTGTTGATTGAGAAGTACGAGCCC AACAGCAGCCTCGCCAAGAAAGGGCAGATGTCAGTGGATGGATTCATGCGCTACCTGAGCGGAGAAGAAA ATGGAGTCGTTTCACCTGAGAAACTGGATTTGAACGAAGACATGTCTCAGCCCCTGTCTCACTATTTCAT CAATTCCTCACACAACACCTACCTCACAGCTGGCCAGTTGGCTGGGAACTCGTCTGTAGAGATGTATCGC CAGGTGCTTCTGTCTGGATGTCGCTGTGTGGAGCTGGACTGCTGGAAGGGCAGGACCGCTGAGGAAGAGC CTGTCATCACCCATGGATTCACCATGACAACAGAAATATCCTTCAAGGAAGTCATAGAAGCCATCGCAGA GTGTGCGTTCAAGACTTCTCCTTTTCCCATCCTCCTTTCCTTTGAGAACCATGTGGATTCCCCGAAGCAA CAAGCCAAGATGGCCGAGTATTGCCGATTAATCTTTGGTGATGCCCTCCTTATGGAGCCACTGGAAAAAT ACCCACTGGAATCTGGGGTACCTCTTCCAAGCCCTATGGATTTAATGTATAAAATCTTGGTGAAAAACAA GAAGAAGTCACACAAGTCGTCAGAGGGAAGTGGTAAAAAGAAGCTCTCTGAGCAAGCTTCCAACACGTAC AGCGACTCTTCCAGCGTGTTCGAGCCTTCGTCTCCGGGAGCTGGGGAAGCAGATACGGAGAGTGATGACG ATGACGACGATGATGACTGTAAAAAGTCTTCCATGGATGAGGGGACTGCTGGCAGCGAGGCCATGGCCAC AGAAGAGATGTCTAACCTGGTGAACTATATTCAGCCTGTCAAGTTTGAGTCCTTTGAAACTTCAAAAAAA AGAAATAAAAGCTTTGAAATGTCTTCCTTCGTGGAAACCAAAGGACTCGAACAACTCACGAAGTCTCCAG TTGAATTTGTCGAATACAACAAGATGCAGCTTAGCAGGATATATCCCAAAGGAACACGCGTGGACTCATC CAACTACATGCCTCAGCTCTTCTGGAATGCTGGCTGTCAGATGGTGGCGCTCAACTTCCAGACAGTGGAT CTAGCTATGCAGATAAACATGGGCATGTACGAATACAATGGGAAGAGTGGCTACAGGCTGAAGCCAGAGT TCATGAGGAGGCCAGACAAGCATTTTGATCCATTTACTGAAGGAATCGTAGATGGGATAGTGGCCAACAC TTTATCTGTTAAGATTATTTCAGGTCAGTTTCTCTCTGATAAGAAAGTTGGGACTTATGTGGAAGTGGAT ATGTTTGGTTTGCCTGTGGACACAAGAAGGAAGGCATTTAAAACCAAGACATCCCAAGGAAATGCTGTAA ATCCTGTCTGGGAAGAAGAGCCAATTGTATTCAAAAAGGTAGTTCTGCCTTCTCTGGCCTGTTTAAGGAT AGCAGCATATGAGGAAGGAGGCAAATTTATTGGCCACCGGATCTTGCCTGTGCAAGCAATTCGGCCAGGC TATCACTACATCTGCCTGCGGAATGAGAGGAACCAGCCCCTGATGCTGCCAGCTGTCTTTGTCTACATAG AAGTCAAAGACTATGTCCCAGACACGTATGCAGATGTAATTGAAGCATTATCAAACCCAATCCGATATGT CAATCTGATGGAACAGAGAGCTAAGCAGTTGGCTGCATTGACACTGGAGGATGAAGAGGAAGTCAAGAAG GAGGCTGACCCCGGGGAAACGTCATCCGAGGCTCCAAGTGAAACCAGGACAACTCCAGCAGAGAATGGGG TGAATCACACCGCAACCCTTGCACCCAAGCCACCTTCCCAGGCTCCACACAGCCAGCCTGCTCCAGGGTC TGTGAAGGCACCCGCCAAAACAGAGGATCTGATTCAGAGCGTGTTAACAGAAGTAGAGGCGCAGACCATT GAAGAGCTCAAGCAACAGAAATCGTTCGTGAAACTTCAAAAGAAGCACTACAAAGAAATGAAAGACCTGG TCAAGAGACACCACAAGAAAACCACCGAGCTCATCAAGGAGCATACCACCAAGTACAATGAGATTCAGAA TGACTACTTGAGAAGGAGGGCAGCCTTGGAAAAGTCCGCCAAAAAGGATAGCAAGAAGAAATCTGAACCC AGCAGCCCAGATCATGGCTCATCCGCCATTGAGCAAGACCTCGCGGCCCTGGATGCAGAAATGACTCAGA AGTTGATAGACTTGAAAGACAAGCAACAACAGCAGCTGCTTAATCTTCGGCAAGAGCAGTATTACAGTGA GAAGTACCAGAAGCGGGAGCACATTAAATTGCTCATTCAGAAGTTGACAGATGTTGCTGAAGAGTGTCAG AACAATCAGTTGAAGAAGCTGAAGGAAATCTGCGAGAAAGAGAAGAAGGAATTAAAGAAGAAAATGGATA AGAAGAGGCAGGAGAAGATAACAGAAGCCAAGTCCAAAGACAAAAGCCAGATGGAAGAGGAGAAGACAGA GATGATCCGATCATACATCCAGGAGGTGGTGCAGTACATCAAGAGGTTAGAGGAAGCACAAAGTAAAAGA CAAGAAAAACTTGTGGAAAAACACAAGGAGATCCGCCAGCAGATCCTGGATGAGAAGCCCAAGCTGCAGA TGGAGCTGGAGCAAGAATACCAAGACAAGTTCAAAAGACTGCCCCTGGAGATTCTGGAGTTTGTACAGGA AGCCATGAAAGGGAAGGTTAGTGAGGACAGCAATCACGGCTCTGCCCCTCCCTCGCTGGCCTCAGACCCT GCTAAGGTGAACCTCAAGTCTCCCTCCAGTGAGGAGGTACAAGGAGAGAACGCGGGAAGAGAGTTTGATA CTCCTCTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210622 representing NM_001077641 Red=Cloning site Green=Tags(s) MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVK DARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATN LLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPD VYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEP NSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYR QVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQ QAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTY SDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFETSKK RNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVD LAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPG YHYICLRNERNQPLMLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKK EADPGETSSEAPSETRTTPAENGVNHTATLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI EELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEP SSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQ NNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKR QEKLVEKHKEIRQQILDEKPKLQMELEQEYQDKFKRLPLEILEFVQEAMKGKVSEDSNHGSAPPSLASDP AKVNLKSPSSEEVQGENAGREFDTPL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001077641
ORF Size	3648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001077641.1, NP_001071109.1
RefSeq Size	5217 bp
RefSeq ORF	3651 bp
Locus ID	24654
UniProt ID	P10687
MW	138.3 kDa
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function]

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