Atad2 (NM_001134879) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210547 representing NM_001134879 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTCCTCCGCAGCAGCCTGGAGCTGCACAGTCACTCCACCATCTCTTCGGCCACCGACTCTCTGG ACCTGTCCAGTGAGTTCCTCAGTCTGGAACACAGCGGCCGGAGGCGGCCCAGCTTGGCCCGAGCCGCGGG GAAATCTACTGTGCCCAAAGCGGAGGCGGCGGGCGATGGATCCTCAGTTAAGGGAGTTGAAATCTGCCAC AATACACGTTCTTTAAGGTCTTTGAGAAAGCAAGCACAAACTTCTTCGGATTCTAGTTCTGATAAGAATG TGGAAAGAACAGAGAAATACTGCAATGGCAGACATTTCACAAGGCAGTTGGCTCAACAGCAGGCTGATAA AAAAAAAGAAGAATGCCAAGAAGACAAAGCTATTCCAGTTATTCGCTCACTGAGGAATAGAAACATTGCT CCGAGTACAGAACATTTGCATGAACAAAGTGGAGATGTTGAAGTTCGAAGAAGTTGTAGGATTAGAACAT GTCGTTACAGTACCATGAACCAGTCTGTGCTATTTGATAAGCTTATAACAAACACTGCTGAAGCTGTACT TCAAAAGATGGATGACATGAAGAAGATGCGTAGACAGCGAATGAAGAAACTTGAAGATCTGGGCGTGTTC AATGAAACAGAAGAGAGTAATCTTACAATGTACACAAGAGGAAAACGAAAGGCTATTCAGCAGGCTGATG AAGAAACAACGGATAACCAAGACGGCAGTGTGGAGTCATCTGAAGAGGGTGAAGAGCAAGAGGATGATGA TGGTGAAGATGAAGAAGATGATGAAGATGAAGAAGAAGATGGAGAAGAAGATAATCAGAAAAGATACTAC CTTAGGCAGAGAAAAGCAACTGTTTACTACCAGGCTCCACTGGAAAAACCTCGTCACCAGAGAAAGCCCA ACATGTTTTATAGTGGCCCAGCTTCTCCTGCAAGACCAAGATACCGGTTGTCGTCTTCAGGACCTAGAAG TCCCTATTGTAAACGAATGAACAGGCGAAGGCATGCCATCCACAGTAGTGATTCTACTTCCTCCTCCTCC TCCTCTGAAGATGAATGCTTTGAGAGGAGAAGAAAAAGGAACCGAAATAGAGCTATTAACAGGTGCCTCC CACTAAATTTTCGGAAAGATGAAATAAGAGGAATTTATAAAGATCGAATGAAAATTGGAGCAAACCTTGC TGATGTTGATCCAATGCAACTGGATTCTTCAGTGCGATTTGATAGTGTTGGCGGCCTGTCCAGTCACATT GCAGCTCTAAAAGAGATGGTGCTGTTTCCCTTACTTTATCCAGAAGTCTTTGAAAAGTTTAAAATTCAAC CTCCAAGAGGTTGTTTGTTTTATGGACCACCTGGAACTGGAAAAACACTGGTTGCTAGAGCACTTGCCAA TGAGTGTAGTCGAGGGGATAAAAGAGTGGCGTTTTTCATGAGGAAAGGTGCTGATTGTCTGAGTAAATGG GTAGGAGAATCTGAAAGACAGCTACGATTGCTATTTGATCAGGCCTATCAGATGCGCCCAGCAATTATAT TCTTTGATGAAATCGATGGTCTGGCTCCAGTACGATCTAGCAGGCAAGATCAGATTCACAGTTCTATTGT TTCAACCCTGTTAGCTCTTATGGATGGTTTGGACAGCAGAGGAGAAATTGTGGTCATTGGAGCTACCAAC AGACTAGATTCCATAGACCCTGCTTTACGAAGGCCTGGTCGATTTGACAGAGAATTCCTTTTCAGTCTAC CTGATAAAGATGCTCGAAAAGAGATTCTGAAGATTCATACAAGAGATTGGAATCCAAAACCAGTGGACAT GTTTTTAGAAGAACTAGCAGAAAACTGTGTTGGATACTGTGGTGCAGATATTAAGTCAATATGTGCCGAA GCTGCTTTATGTGCCCTGCGTCGACGGTACCCTCAGATCTATACCACCAGTGAGAAACTACAGTTGGATC TCTCTTCAATTAATATCTCAGCTAAGGATTTTGAGACAGCTATGCAAAAGATAATACCAGCTTCCCAGAG AGCTGTGACATCACCGGGACAGGCACTGTCTGCCATTGTGAAACCACTTCTGCAAAATACTGTTCATATG ATCTTAGAAGCCCTACAGAAAGTGTTTCCACATGTGGAAGTTGGAACAAACAAAGCTTTAAATTCAGATG TTTCTTTCCCTTTGCTAGAGAGTGATTTGGCATACAGTGATGATGATACACCGTCAATGTATGAAAATGG ACTTTCTCAAAAAAATTTTAATCAGGCAAAAGAAAATTTAAATTTTCTTCATTTAAATAGAAATGCCTGT TACCAGCCTATGTCTTTTCGACCAAGACTATTGATAGTGGGAGAACCAGGATTTGGACAGAGTTCTCACT TGGCACCAGCTGTAATCCATGCTTTGGAAAAATTTACTGTATATACATTAGACATTCCTGTTCTTTTTGG CATTAGTACTACATCTCCTGAAGAGGCATGCTCACAAATGATTCGTGAAGCTAAGAGAACAGCACCCAGC ATAGTCTATGTCCCGCATGTCCACTTGTGGTGGGAAATCGTTGGCCCGACACTCAGGGCCACGTTCACAA CATTATTACAGAATATCCCTTCGTTTGCTCCAGTGTTACTGCTTGCAACCTCTGAGAGACCATACTCCGC TCTGCCTGAAGAGGTACAAGAATTGTTTACCCATGATTATGGAGAAATTTTTAATGTCCAGTTACCAGAT AAAGAAGAACGAACCAAATTTTTTGAAGACTTAATTTTAAAACAAGCTGCAAAACCTCCTGTGTCACAAA AGAAAGCAGTTCTGCAGGCCTTGGAGGTGCTCCCGGTGGCACCTCCACCTGAACCAAGACCACTGACAGC AGAAGAAGTTAAACGGCTTGAAGAACAAGAAGAAGATACATTTAGAGAACTCAGGATTTTCTTAAGAAAT GTCACACATAGACTTGCTATTGATAAGCGATTCCGAGTCTTCACTAAGCCTGTTGACCCTGATGAGGTTC CTGATTATGTCACTGTCATAAAGCAGCCAATGGACCTTTCATCTGTAATCAGTAAAATTGATTTACATAA ATACCTGACTGTGAAAGACTATTTGAAGGATATTGATCTAATCTGTAGTAACGCTTTAGAATACAATCCA GATAGAGATCCTGGAGATCGTCTTATTAGGCACAGAGCTTGTGCTTTAAGAGACACTGCCTATGCAATCA TTAAAGAAGAGCTTGATGAAGACTTTGAGCAGCTTTGTGAAGAAATTCAGGAATCTAGGAAGAAAAGAGG TTGTAGCTCTTCCAAATATGCACCATCTTATTACCACGTCATGCCAAAGCAAAATTCCACTCCTGTCGGT GATAAAAGGTCAGATCAAGAGCAGAAGGAGAAGCTGAAGGCACCTTGTACTCCCATGGCAAGCAGCACCC CTGCTCGGTTGAAGAGAAAATTCCGGAAAAAGTCAAAGTGGCATGTAGGCACCAAAATAAAACGAAGGAA AATTTCACAAGCTAAAGACAGTAGCCAGACTGCTGTGAACAGTGCAAGCAGGAGTGACTCAGAAGACAGT CAGCATACACGTACAGAGCTCGGAAACACAGGGGAGTCCTCAGTAGAAGAAAGTGACAAGCAGAACACCT CTGAAAGCAACATAGACTTGAAAAATAACTCAAGTTCCTCCAATATTGAGAATGAACTTGAAGAGCCTAA GCAAACTGCAGAGGGCACAGAGCTGAAAAAAGCCAGGATTGTTTGTAGTGGCGATGCCGCTACCTCCCAG GTCACAGACATTGCTGAAGAAAATGAATCAAAAGAAATGGGGTTTCTGCGAATGACTCGAGCTAGACATT CCCAGGTAGAGCAGCAGCAGCTGATCGGTATGGAAAAGGCTCTGGCGATTCTCTCTCAGCCTACACCTTC ACTTGTTGTGGACCATAAGCACTTAAAAAATCTTTTGAAGACTGTTGTTAAAAAAAGTCAGGAATATAAT ATATTCCAACTGGAAAATTTGTATGCAGTCCTCAGCCAGTGTATTTATGAGCACCGCAGGGACTATGACA AAACAGCACTTGTTCAGAAAATGGAGCAAGCAGTAGAGAACTTCAGTTGTTCCAGATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210547 representing NM_001134879 Red=Cloning site Green=Tags(s) MVVLRSSLELHSHSTISSATDSLDLSSEFLSLEHSGRRRPSLARAAGKSTVPKAEAAGDGSSVKGVEICH NTRSLRSLRKQAQTSSDSSSDKNVERTEKYCNGRHFTRQLAQQQADKKKEECQEDKAIPVIRSLRNRNIA PSTEHLHEQSGDVEVRRSCRIRTCRYSTMNQSVLFDKLITNTAEAVLQKMDDMKKMRRQRMKKLEDLGVF NETEESNLTMYTRGKRKAIQQADEETTDNQDGSVESSEEGEEQEDDDGEDEEDDEDEEEDGEEDNQKRYY LRQRKATVYYQAPLEKPRHQRKPNMFYSGPASPARPRYRLSSSGPRSPYCKRMNRRRHAIHSSDSTSSSS SSEDECFERRRKRNRNRAINRCLPLNFRKDEIRGIYKDRMKIGANLADVDPMQLDSSVRFDSVGGLSSHI AALKEMVLFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSRGDKRVAFFMRKGADCLSKW VGESERQLRLLFDQAYQMRPAIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDSRGEIVVIGATN RLDSIDPALRRPGRFDREFLFSLPDKDARKEILKIHTRDWNPKPVDMFLEELAENCVGYCGADIKSICAE AALCALRRRYPQIYTTSEKLQLDLSSINISAKDFETAMQKIIPASQRAVTSPGQALSAIVKPLLQNTVHM ILEALQKVFPHVEVGTNKALNSDVSFPLLESDLAYSDDDTPSMYENGLSQKNFNQAKENLNFLHLNRNAC YQPMSFRPRLLIVGEPGFGQSSHLAPAVIHALEKFTVYTLDIPVLFGISTTSPEEACSQMIREAKRTAPS IVYVPHVHLWWEIVGPTLRATFTTLLQNIPSFAPVLLLATSERPYSALPEEVQELFTHDYGEIFNVQLPD KEERTKFFEDLILKQAAKPPVSQKKAVLQALEVLPVAPPPEPRPLTAEEVKRLEEQEEDTFRELRIFLRN VTHRLAIDKRFRVFTKPVDPDEVPDYVTVIKQPMDLSSVISKIDLHKYLTVKDYLKDIDLICSNALEYNP DRDPGDRLIRHRACALRDTAYAIIKEELDEDFEQLCEEIQESRKKRGCSSSKYAPSYYHVMPKQNSTPVG DKRSDQEQKEKLKAPCTPMASSTPARLKRKFRKKSKWHVGTKIKRRKISQAKDSSQTAVNSASRSDSEDS QHTRTELGNTGESSVEESDKQNTSESNIDLKNNSSSSNIENELEEPKQTAEGTELKKARIVCSGDAATSQ VTDIAEENESKEMGFLRMTRARHSQVEQQQLIGMEKALAILSQPTPSLVVDHKHLKNLLKTVVKKSQEYN IFQLENLYAVLSQCIYEHRRDYDKTALVQKMEQAVENFSCSRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134879
ORF Size	4119 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134879.1, NP_001128351.1
RefSeq Size	5557 bp
RefSeq ORF	4122 bp
Locus ID	314993
MW	156 kDa

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