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Hace1 (NM_001108539) Rat Tagged ORF Clone
CAT＃: RR210275
- TrueORF®
Hace1 (Myc-DDK-tagged ORF) - Rat HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), (10 ug)
"NM_001108539" in other vectors (3)
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CNY 11,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210275 representing NM_001108539
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGAGCGATGGAGCAGCTCAACCGTCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGCTGC CCGAGGATAACGAGACTGCTGTTTACACATTAATGCCAATGGTGATGGCGGATCAGCACAGGTCTGTTTC TGAACTCCTATCAAATTCAAAATTTGATGTCAATTATGCATTTGGACGTGTGAAGAGAAGCTTGCTTCAC ATTGCAGCAAACTGTGGATCTGTGGAGTGCTTGGTTCTTCTGTTAAAGAAAGGAGCAAACCCCAACTACC AAGACATTTCAGGCTGTACGCCCCTTCATCTGGCCGCTAGGAACGGACAGAAGAAATGCATGAGTAAATT ACTGGAGTACAGTGCAGACGTCAACATTTGCAATAATGAAGGCCTTACCGCAATCCACTGGCTGGCTGTG AATGGAAGGACAGAGCTACTCCATGACCTGGTGCAGCACGTCACGGATGTGGATGTGGAGGATGCAATGG GGCAGACAGCATTACATGTGGCCTGCCAGAACGGGCACAAAACGACCGTGCAGTGTCTGCTGGACAGCGG TGCTGACATCAACCGGCCGAATGTGGCAGGAGCTACGCCACTGTACTTTGCTTGCAGTCATGGCCAGAGA GATACTGCGCAGATTCTTCTATTACGAGGAGCCAAATATTTACCAGATAAAAACGGAGTAACCCCTCTGG ACCTGTGTGTGCAGGGTGGCTATGGACAGACTTGCGAAGTGTTAATTCAGTATCACCCCAGGCTTTTCCA GACGATTGTTCAAATGACGCAGAATGAAGACCTTCGGGAGAACATGTTACGGCAAGTTCTGCAGCATTTG TCTCAGCAGAGTGAAAGCCAGTACCTAAAGATCCTGACAGGTCTTGCTGAAGTTGCTACAACAAATGGCC ATAAACTGCTTAGTCTGTCCAGCAGCTACGAGGCGCAGATGAAGAGCCTCCTGCGGATCGTGAGGATCTT CTGCCACGTCTTCCGCATTGGCCCCTCGTCTCCCAGTAACGGCATGGATATGGGCTACAATGGGAATAAG ACTCCAAGGAGCCAGGTGTTCAAGCCTTTGGAATTGCTTTGGCACTCTCTGGATGAGTGGTTGGTTTTAA TAGCCACAGAATTAATGAAGAACAAAGAGGACTCAGCGGATATCACCTCCATTTTACTGAAACAGAAAGG CCAGGATCAAGAGGCCTCTTGTATTTCAGCGTTTGAGCCACCAGGACCTGGGAGCTATGAACGCCTGTCC ACTGGTCCCGGGGAATCCAAACCAGATGTGCTTGCCGGGAAGCAGGAAGCCAGTGCGGACTGTCAGGATG TCATTTCTGTGACGGCCAACCGGCTAAGTGCTGTCATTCAAGCCTTTTACATGTGCTGCTCCTGTCAGAT GCCTCCAGGAATGACGTCGCCTCGGTTCATTGAGTTTGTCTGCAAGCATGATGAAGTGCTAAAGTGCTTT GTGAATAGAAATCCCAAAATTATATTTGACCATTTTCACTTTCTTCTTGAATGTCCTGAACTGATGTCGA GATTCATGCACATCATAAAGGCACAGCCATTCAAAGACCGCTGTGAATGGTTCTATGAGCATTTGCACTC AGGACAGCCAGACTCAGACATGGTGCACAGACCAGTGAGTGAAAACGACATTCTCCTGGTTCACAGAGAT TCTATTTTTAGGAGTAGCTGTGAAATCGTATCAAAGGCCAACTGTGCAAAGCTGAAGCAGGGGATCGCTG TGCGCTTTCATGGAGAGGAAGGCATGGGTCAAGGTGTTGTACGTGAATGGTTTGATATTCTGTCTAATGA GATAGTCAATCCTGACTATGCACTGTTTACCCAGTCAGCAGATGGAACAACTTTTCAGCCCAATAGCAAC TCCTATGTCAATCCTGATCACTTGAACTATTTCCGGTTTGCTGGACAGATCTTGGGATTAGCTTTGAATC ACAGGCAGTTGGTCAATATTTACTTCACACGATCCTTCTACAAACACATTCTTGGTATTCCTGTGAATTA CCAAGATGTAGCGTCTATCGATCCAGAATATGCCAAAAATCTGCAGTGGATTTTAGACAATGACATTAGT GACCTGGGTCTAGAACTGACTTTCTCTGTTGAGACTGATGTGTTTGGAGCTATGGAAGAGGTACCGTTAA AGCCTGGGGGTGGAAGTATTCTTGTGACACAAAATAACAAAGCGGAGTACGTCCAGCTGGTTACTGAACT CCGGATGACAAGAGCCATTCAGCCTCAGATCAATGCGTTTTTACAGGGTTTCCATATGTTCATCCCTCCT TCCCTCATACAGCTGTTCGACGAGTATGAATTGGAGCTACTGCTCTCTGGCATGCCGGAAATTGACGTGA ATGATTGGATCAAAAATACAGAATACACAAGTGGCTATGAAAGGGAAGATCCAGTTATTCAGTGGTTCTG GGAGGTTGTGGAGGACATGACTCAAGAAGAGCGGGTCCTTCTCTTACAGTTCGTGACTGGCAGTTCCAGG GTCCCGCATGGTGGCTTTGCCAATATCATGGGTGGCAGTGGTTTGCAAAACTTTACAATTGCTGCTGTGC CATATACTCCAAATCTTTTACCAACTTCCAGCACGTGCATCAACATGCTCAAGTTACCTGAATACCCAAG TAAAGAGATCCTCAAGGACAGACTCCTCGTAGCTCTGCATTGTGGCAGCTACGGCTATACAATGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210275 representing NM_001108539
Red=Cloning site Green=Tags(s) MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLH IAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAV NGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVAGATPLYFACSHGQR DTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHL SQQSESQYLKILTGLAEVATTNGHKLLSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNK TPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLS TGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCF VNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRD SIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSN SYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDIS DLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPP SLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSR VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108539 |
| ORF Size | 2727 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108539.3, NP_001102009.2 |
| RefSeq Size | 3441 bp |
| RefSeq ORF | 2730 bp |
| Locus ID | 361866 |
| UniProt ID | D3ZBM7 |
| MW | 102.2 kDa |
| Gene Summary | E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210275 | Hace1 (untagged ORF) - Rat HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), (10 ug) |
CNY 11,690.00 |
|
| RR210275L3 | Lenti ORF clone of Hace1 (Myc-DDK-tagged ORF) - Rat HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), (10 ug) |
CNY 13,400.00 |
|
| RR210275L4 | Lenti ORF clone of Hace1 (mGFP-tagged ORF) - Rat HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), (10 ug) |
CNY 14,820.00 |
