Akap6 (NM_022618) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mAkap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210104 representing NM_022618 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAACCATGAGCGTGACACTTTCCCCACTGAGGTCACAGGGCCCAGATCCCATGGCGACGGATGCTT CACCCATGGCCATCAACATGACACCCACTGTGGAGCAGGAGGAAGGAGAGGGAGAGGAAGCCGTGAAGGC CATAGACGCTGAGCAGCAGTATGGAAAGCCACCTCCGCTCCACACAGCAGCCGACTGGAAGATTGTCCTG CACTTACCTGAGATTGAGACCTGGCTCCGGATGACCTCAGAGAGGGTCCGTGACCTGACCTACTCAGTCC AGCAGGATGCAGACAGCAAGCATGTGGATGTGCATCTAGTTCAGCTGAAGGACATTTGTGAGGATATTTC TGACCATGTGGAGCAGATCCATGCCCTCCTTGAGACGGAGTTTTCCCTAAAGCTGCTGTCCTACTCGGTC AACGTCATCGTAGACATCCACGCAGTACAGCTGCTCTGGCACCAGCTCCGCGTATCCGTGCTGGTCCTCC GGGAGCGCATCCTACAAGGTCTGCAGGACGCCAATGGCAACTACACCAGGCAGACTGACATTCTGCAAGC GTTCTCTGAAGAAACAACGGAGGGCCGGCTTGATTCCCTTACAGAAGTGGACGACTCAGGGCAGTTAACT ATCAAATGTTCACAGGATTACTTGTCTCTGGATTGTGGCATTACCGCATTTGAACTCTCCGACTACAGTC CAAGTGAGGATCTGCTTGGTGGCCTGGGCGACATGACCACCAGCCAGGCCAAAACTAAATCTTTTGACTC TTGGAGCTACAGTGAGATGGAGAAAGAGTTCCCTGAGCTTATCCGAAGCGTTGGGCTGCTTACAGTGGCC ACCGAGCCTGTCCCTTCCAGCTGTGGAGAAGCCAATGAGGATTCATCTCAAGCGTCCCTTTCAGATGATC ACAAAGGTGAACACGGGGAAGACGGTGCTCCCGTACCTGGACAGCAGCTGGACTCAACGGTGGGAATGTC TTCCTTAGACGGCACGCTGGCAAATGCTGCCGAACACCCTTCGGAGACAGCAAAACAAGACTCTACTTCC TCCCCACAGCTTGGTGCGAAGAAAACCCAGCCTGGTCCTTGTGAAATTACGACTCCCAAGAGATCCATCC GCGATTGCTTTAATTATAACGAGGACTCCCCCACACAGCCCACATTACCCAAAAGAGGGCTTTTTCTAAA AGAAACTCAAAAGAATGAGCGCAAAGGCAGTGACAGGAAGGGGCAGGTGGTTGATTTAAAGCCTGAACTG AGCAGAAGCACCCCTTCCCTGGTGGACCCCCCTGACAGATCGAAGCTCTGCCTAGTGTTGCAGTCCTCCT ACCCCAGCAGCCCTTCTGCTGCCAGCCAGTCCTATGAATGTTTGCACAAGGTGGGGCTCGGCAATCTTGA AAACATAGTCAGAAGTCACATTAAAGAAATTTCTTCCAGTCTGGGAAGGCTTACTGACTGCCATAAAGAG AAATTGCGACTGAAAAAGCCACACAAGACCTTGGCCGAAGTGTCTCTGTGCAGAATCCCTAAACAGGGAG GCGGTTCAGGAAAGCGATCTGAGAGCACCGGGAGCTCAGCAGGGCCCAGCATGGTATCACCTGGAGCTCC CAAAGCCACGATGAGACCAGAAACAGATTCTGCGTCTACAGCCTCAGGTGGCCTGTGCCACCAGAGAAAT CGCAGTGGACAATTGCCAGTGCAGTCGAAGGCCTCCAGTTCACCCCCTTGCAGTCACAGCAGTGAATCTT CTCTTGGCTCAGATAGCATCAAATCCCCGGTTCCTCTTCTTTCAAAAAACAAAAGCCAAAAAAGCTCCCC ACCTGCTCCATGTCACGCCACACAGAACGGTCAGGTGGTGGAGGCCTGGTACGGCTCTGATGAGTACCTA GCGCTGCCCTCTCACCTGAAGCAGACGGAGGTGTTAGCTCTCAAGCTGGAGAGCCTAACCAAGCTCCTAC CCCAGAAACCCAGAGGAGAGACCATCCAGGATATTGATGACTGGGAACTGTCTGAAATGAATTCAGATTC CGAAATCTATCCAACATACCACATCAAGAAAAAACACACGAGACTGGGCACAGTGTCTCCAAGCTCATCC AGCGACATAGCCTCATCTCTCGGGGAGAGCATTGAATCCGGGCCCCTGAGTGACATTCTTTCTGACGAGG ACTTATGTCTGCCCCTCTCCAGCGTGAAAAAGTTCACTGACGAGAAATCAGAGAGACCTTCATCCTCCGA GAAGAACGAGAGCCATTCTGCAACAAGATCAGCTTTGATTCAGAAACTAATGCACGATATTCAGCACCAA GAGAACTATGAAGCCATCTGGGAAAGAATTGAGGGGTTTGTGAACAAGCTGGATGAATTCATTCAGTGGC TAAACGAAGCCATGGAGACCACCGAGAACTGGACTCCTCCTAAAGCCGAGACCGACAGCCTCCGGCTGTA CCTGGAGACACACTTGAGTTTTAAGTTGAACGTAGACAGCCACTGTGCCCTCAAGGAAGCCGTGGAGGAA GAAGGACACCAACTTCTTGAGCTCGTTGTATCTCACAAAGCAGGACTGAAGGACACGCTGAGGATGATTG CGAGTCAATGGAAGGAGCTGCAGAGGCAAATCAAACGGCAACACAGCTGGATTCTCAGAGCCCTGGACAC CATCAAAGCCGAGATACTGGCTACTGATGTGTCTGTGGAGGACGAGGAGGGGACGGGAAGCCCCAAGGCC GAGGTTCAGCTCTGCCACCTGGAAACACAGAGAGACGCCGTGGAACAGATGTCCCTGAAGCTGTACAGCG AGCAGTACACCAGCGGGAGCAAGAGGAAGGAAGAGTTTGCCAACATGTCGAAAGCGCACGCGGAGGGAAG CAATGGGCTTCTGGACTTTGATTCAGAATATCAGGAGCTCTGGGATTGGCTGATTGACATGGAGTCCCTC GTGATGGACAGCCACGACCTGATGATGTCAGAGGAGCAGCAGCAGCATCTTTACAAGAGGTACAGTGTGG AAATGTCCATCAGGCATCTGAAAAAGTCAGAGCTACTCAGCAAGGTTGAAGCTTTGAAGAAAGGTGGCCT TTCACTACCAGACGATATCCTGGAAAAAGTGGATTCAATTAATGAAAAATGGGAGCTGCTTGGGAAAACC CTAAGAGAGAAGATACAGGACACAATAGCGGGGCACAGTGGGTCGGGCCCACGTGACCTGCTATCTCCTG AAAGCGGAAGCCTGGTAAGGCAGCTGGAGGTCAGGATCAAAGAGCTGAAAAGGTGGCTAAGAGATACAGA GCTTTTCATCTTCAATTCCTGTCTGAGACAAGAGAAGGAAGGAACAAGCGCCGAGAAACAGCTCCAATAC TTTAAGTCGCTCTGTCGTGAGATCAAGCAGCGGCGTCGAGGAGTGGCCTCCATTCTGAGGTTGTGCCAGC ACCTTCTGGATGACCGGGACACGTGCAACCTGAACGCAGATCACCAGCCCATGCAGCTGATCATTGTAAA CCTCGAGAGGCGGTGGGAGGCCATCGTCATGCAAGCTGTCCAGTGGCAAACACGGTTACAAAAGAAGATG GGGAAGGAATCCGAGACTTTGAATGTGATTGATCCTGGCTTGATGGACCTGAATGGAATGAGTGAGGATG CCCTGGAATGGGATGAAACAGACATAAGTAACAAACTCATTAGTGTGCATGAAGAATCAAACGACCTTGA TCAAGACCCAGAGCCTATGCTACCCGCAGTGAAGCTTGAAGAGACACACCACAAGGACTCTGGTTATGAA GAGGAGGCAGGTGACTGTGGAGGGTCTCCGTATACCTCAAATATCACTGCACCTTCCAGCCCACACATTT ACCAAGTGTACAGTCTTCACAATGTGGAGCTCCACGAGGACAGCCACACTCCATTTCTGAAAAGCAGCCC TAAGTTCACAGGCACAACACAGCCTACTGTTTTAACTAAGAGCCTCAGCAAGGACTCTTCCTTTTCATCT ACAAAATCGTTACCAGACCTTCTAGGGGGTTCCGGTTTGGTGAGGCCTTACTCGTGTCACAGTGGAGACT TGAGCCAGAATTCAGGCAGTGAGAGTGGAATTGTCAGCGAAGGAGACAACGAGATGCCGACCAACTCTGA CATGAGCTTGTTCAGTATGGTAGACGGGTCCCCAAGTAACCCTGAAACGGAGCATCCGGACCCACAAATG GGAGATGCAGCCAATGTGCTAGAGCAAAAGTTTAAAGACAACGGGGAAAGCATTAAGCTTTCAAGTGTCT CTCGGGCATCCGTCTCACCAGTGGGTTGTGTAAATGGAAAAGCAGGGGATTTAAACAGTGTTACCAAACA CACTGCTGATTGTTTGGGAGAAGAACTACAAGGAAAACATGACGTGTTTACATTTTATGATTACTCGTAC CTCCAAGGCTCAAAACTCAAATTACCAATGATAATGAAACAGCCACAGAGTGAAAAGGCACACGTGGAGG ATCCCCTTCTTGGTGGTTTTTATTTTGATAAAAAGTCCTGCAAAGCTAAACATCAGGCTTCAGAGTCACA ACCAGATGCGCCTCCCCACGAAAGGATTCTGGCAAGCGCGCCCCACGAGATGGGACGCAGCGCATACAAA AGTAGCGACATAGAGAAGACATTCACGGGCATTCAGAGTGCCAGACAGCTCTCCCTTCTATCTCGTAGCT CATCTGTAGAGTCCCTTTCTCCAGGGGGTGATTTGTTTGGATTGGGAATCTTTAAAAATGGCAGTGACAG CCTCCAGCGGAGCACTTCTTTAGAAAGTTGGTTGACATCCTATAAGAGCAATGAGGATCTCTTTAGCTGT CACAGCTCTGGGGACATAAGTGTGAGCAGTGGCTCAGTTGGTGAGCTGAGTAAGAGGACGTTAGACCTTC TGAATCGCCTGGAGAATATACAGAGCCCCTCGGAGCAAAAGATCAAGCGGAGTGTTTCTGACATGACTCT ACAAAGCAGTTCCCAAAAGATGCCCTTCGCTGGCCAGATGTCACTGGATGTCGCATCCTCCATCAATGAA GACTCTCCGGCATCTCTTACAGAACTGAGTAGTAGCGATGAGCTCTCTCTTTGCTCGGAGGACATTGTGT TACACAAAAACAAGATCCCAGAATCCAACGCATCATTCAGGAAGCGCCTGAATCGCTCAGTGGCTGATGA GAGCGACGTCAATGTTAGCATGATTGTCAATGTGTCCTGCACCTCTGCTTGCACTGATGATGAAGATGAC AGCGACCTCCTCTCCAGCTCCACTCTCACCTTAACTGAAGAAGAGCTGTGCCTCAAAGATGAGGATGACG ACTCCAGTATTGCAACAGATGATGAAATTTATGAAGAGAGCAACCTGATGTCTGGGCTGGACTACATAAA GAATGAACTGCAGACTTGGATAAGACCAAAACTTTCCTTGACGAGAGAAAAGAAACGGTCCGGTGTCACT GATGAAATAAAGGTCAATAAAGATGGGGGAGGCAATGAGAAGGCCAATCCCTCGGACACCCTGGACATCG AGGCCCTTCTCAATGGCTCCATAAGATGTCTTTCCGAAAACAACGGGAATGGTAAGACTCCGCCCAGAAC TCATGGCTCAGGAACCAAAGGTGAAAATAAGAAAAGTACGTATGACGTTAGTAAGGATCCGCACGTGGCT GACATGGAAAATGGCAATATTGAAAGTACCCCAGAAAGAGAAAGGGAGAAGCCACAAGGGCTTCCAGAGG TGTCAGAGAACCTTGCTTCAAATGTGAAAACGATTTCTGAATCTGAGCTCAGCGAGTATGAAGCAGTAAT GGATGGTTCTGAGGATTCAAGTGTTGCCAGAAAGGAATTTTGTCCCCCAAATGACAGACATCCTCCACAG ATGGGTCCCAAACTCCAGCATCCCGAAAATCAAAGTGGCGACTGCAAGCCAGTCCAGAACCCTTGCCCGG GGCTACTGTCGGAAGCTGGCGTTGGAAGCAGGCAAGACAGCAATGGACTAAAATCTTTGCCTAACGATGC ACCAAGTGGGGCTAGAAAACCTGCCGGTTGCTGCCTGCTGGAGCAGAATGAGACAGAGGAAAGTGCTTCT ATCAGCAGCAACGCTTCCTGTTGCAACTGCAAGCCAGATGTTTTCCATCAAAAAGATGATGAAGATTGTT CAGTACATGACTTTGTTAAGGAAATCATTGACATGGCATCAACAGCCCTAAAAAGTAAGTCACAGCCTGA AAGTGAGGTGGCCGCACCCACATCACTAACCCAAATTAAGGAGAAGGTGTTAGAGCATTCGCACCGGCCC ATACACCTGAGAAAGGGGGACTTTTACTCCTACTTATCACTTTCGTCCCACGACAGTGACTGTGGGGAGG TCACCAATTACATAGATGAGAAGAGCAGTACTCCATTGCCACCGGACGCTGTGGACTCTGGCTTAGATGA CAAGGAAGACATGGACTGCTTCTTTGAAGCTTGTGTTGAGGATGAGCCTGTCAATGAGGAAGCTGGTCTC CCCGGTGCCCTTCCCAATGAATCAGCCATCGAGGATGGAGCAGAGCAAAAGTCAGAACAAAAGACAGCCA GCTCTCCTGTGCTCAGTGACAAGACAGACCTGGTGCCTCTTTCAGGACTTTCCCCTCAGAAGGGAGCTGA TGATGCAAAGGAAGGAGATGATGTGTCTCACACTTCCCAGGGCTGTGCAGAGAGCACAGAGCCTACCACC CCCTCAGGAAAGGCCAATGCAGAGGGGAGGTCAAGAATGCAAGGTGTATCAGCAACGCCAGAAGAAAACG CTGCTTCGGCCAAACCGAAAATTCAAGCTTTCTCTTTGAATGCAAAACAGCCAAAAGGCAAGGTTGCCAT GAGGTATCCCAGCCCCCAAACTCTAACCTGTAAAGAGAAGCTCGTAAACTTTCATGAAGATCGACACAGT AACATGCATAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210104 representing NM_022618 Red=Cloning site Green=Tags(s) MLTMSVTLSPLRSQGPDPMATDASPMAINMTPTVEQEEGEGEEAVKAIDAEQQYGKPPPLHTAADWKIVL HLPEIETWLRMTSERVRDLTYSVQQDADSKHVDVHLVQLKDICEDISDHVEQIHALLETEFSLKLLSYSV NVIVDIHAVQLLWHQLRVSVLVLRERILQGLQDANGNYTRQTDILQAFSEETTEGRLDSLTEVDDSGQLT IKCSQDYLSLDCGITAFELSDYSPSEDLLGGLGDMTTSQAKTKSFDSWSYSEMEKEFPELIRSVGLLTVA TEPVPSSCGEANEDSSQASLSDDHKGEHGEDGAPVPGQQLDSTVGMSSLDGTLANAAEHPSETAKQDSTS SPQLGAKKTQPGPCEITTPKRSIRDCFNYNEDSPTQPTLPKRGLFLKETQKNERKGSDRKGQVVDLKPEL SRSTPSLVDPPDRSKLCLVLQSSYPSSPSAASQSYECLHKVGLGNLENIVRSHIKEISSSLGRLTDCHKE KLRLKKPHKTLAEVSLCRIPKQGGGSGKRSESTGSSAGPSMVSPGAPKATMRPETDSASTASGGLCHQRN RSGQLPVQSKASSSPPCSHSSESSLGSDSIKSPVPLLSKNKSQKSSPPAPCHATQNGQVVEAWYGSDEYL ALPSHLKQTEVLALKLESLTKLLPQKPRGETIQDIDDWELSEMNSDSEIYPTYHIKKKHTRLGTVSPSSS SDIASSLGESIESGPLSDILSDEDLCLPLSSVKKFTDEKSERPSSSEKNESHSATRSALIQKLMHDIQHQ ENYEAIWERIEGFVNKLDEFIQWLNEAMETTENWTPPKAETDSLRLYLETHLSFKLNVDSHCALKEAVEE EGHQLLELVVSHKAGLKDTLRMIASQWKELQRQIKRQHSWILRALDTIKAEILATDVSVEDEEGTGSPKA EVQLCHLETQRDAVEQMSLKLYSEQYTSGSKRKEEFANMSKAHAEGSNGLLDFDSEYQELWDWLIDMESL VMDSHDLMMSEEQQQHLYKRYSVEMSIRHLKKSELLSKVEALKKGGLSLPDDILEKVDSINEKWELLGKT LREKIQDTIAGHSGSGPRDLLSPESGSLVRQLEVRIKELKRWLRDTELFIFNSCLRQEKEGTSAEKQLQY FKSLCREIKQRRRGVASILRLCQHLLDDRDTCNLNADHQPMQLIIVNLERRWEAIVMQAVQWQTRLQKKM GKESETLNVIDPGLMDLNGMSEDALEWDETDISNKLISVHEESNDLDQDPEPMLPAVKLEETHHKDSGYE EEAGDCGGSPYTSNITAPSSPHIYQVYSLHNVELHEDSHTPFLKSSPKFTGTTQPTVLTKSLSKDSSFSS TKSLPDLLGGSGLVRPYSCHSGDLSQNSGSESGIVSEGDNEMPTNSDMSLFSMVDGSPSNPETEHPDPQM GDAANVLEQKFKDNGESIKLSSVSRASVSPVGCVNGKAGDLNSVTKHTADCLGEELQGKHDVFTFYDYSY LQGSKLKLPMIMKQPQSEKAHVEDPLLGGFYFDKKSCKAKHQASESQPDAPPHERILASAPHEMGRSAYK SSDIEKTFTGIQSARQLSLLSRSSSVESLSPGGDLFGLGIFKNGSDSLQRSTSLESWLTSYKSNEDLFSC HSSGDISVSSGSVGELSKRTLDLLNRLENIQSPSEQKIKRSVSDMTLQSSSQKMPFAGQMSLDVASSINE DSPASLTELSSSDELSLCSEDIVLHKNKIPESNASFRKRLNRSVADESDVNVSMIVNVSCTSACTDDEDD SDLLSSSTLTLTEEELCLKDEDDDSSIATDDEIYEESNLMSGLDYIKNELQTWIRPKLSLTREKKRSGVT DEIKVNKDGGGNEKANPSDTLDIEALLNGSIRCLSENNGNGKTPPRTHGSGTKGENKKSTYDVSKDPHVA DMENGNIESTPEREREKPQGLPEVSENLASNVKTISESELSEYEAVMDGSEDSSVARKEFCPPNDRHPPQ MGPKLQHPENQSGDCKPVQNPCPGLLSEAGVGSRQDSNGLKSLPNDAPSGARKPAGCCLLEQNETEESAS ISSNASCCNCKPDVFHQKDDEDCSVHDFVKEIIDMASTALKSKSQPESEVAAPTSLTQIKEKVLEHSHRP IHLRKGDFYSYLSLSSHDSDCGEVTNYIDEKSSTPLPPDAVDSGLDDKEDMDCFFEACVEDEPVNEEAGL PGALPNESAIEDGAEQKSEQKTASSPVLSDKTDLVPLSGLSPQKGADDAKEGDDVSHTSQGCAESTEPTT PSGKANAEGRSRMQGVSATPEENAASAKPKIQAFSLNAKQPKGKVAMRYPSPQTLTCKEKLVNFHEDRHS NMHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022618
ORF Size	6942 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022618.1, NP_072140.1
RefSeq Size	8827 bp
RefSeq ORF	6945 bp
Locus ID	64553
UniProt ID	Q9WVC7
MW	254.4 kDa
Gene Summary	nuclear membrane anchoring protein; involved in compartmentalizing cAMP-dependent protein kinase and other signaling molecules [RGD, Feb 2006]

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