Ltbp2 (NM_021586) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210089 representing NM_021586 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCACCTCCCTGCGAGGTCTCCGGTGCCCACAGCTCTGCAGCCACTCTGGCGCCATGAGGGCGC CGACCACCGTCCGCTGCTCCGGACGCATCCAAAGGGCGCGTTGGAGGGGCTTCCTGCCACTTGTCCTGGC TCTCTTGATGGGGACAAGTCATGCCCAAAGGGATTCCGTGGGGAGATACGAACCAGCTAGCCGGGATGCC AATCGGTTGTGGCGCCCCGTGGGCAACCACCCCGCAGCGGCTGCAGCCAAGGTGTACAGTCTGTTCCGAG AGCCCGACGCGCCGGTCCCCGGCTTGTCGCCCTCTGAGTGGAATCAGCCGGGCCAGGGGATCCCTGGGAG GCTCGCAGAGGCCGAGGCCAGGAGACCGTCCCGAGCCCAGCAGCTGCGTCGAGTCCAGTCACCTGTCCAG ACTCGGAGAAGCAATCCCCGAGGCCAGCAGCCACCAGCAGCCCGGACCGCACATTCCGTCGTGCGCCTGG CGACCCCTCAGCGACCCGCGGCTGCACGCCGAGGGCGGCTCACCGGGAGAAATGTCTGCGGGGGACAGTG CTGCCCTGGATGGACGACATCGAACAGCACCAACCACTGTATCAAACCTGTGTGTCAGCCTCCCTGTCAG AACCGGGGCTCCTGCAGCCGGCCCCAGCTCTGCATCTGCCGTTCTGGCTTCCGTGGGGCACGCTGCGAGG AGGTCATCCCTGAGGAGGAGTTTGACCCTCAGAATGCCAGGCCTGTGCCCAGACGCTCAGTGGAGGGAGC ACCTGGCCCTCACAGGAGCAGCGAGGCCAGAGGAAGTCTAGTGACCAGAATACAGCCGCTGCTACCACCA CTACCACCACCTCCATCTAGGACCCTCAGCCAGACCCGTCCCCTGCAGCAGCATGCAGGACTGTCCAGAA CAGTTCGTCGTTATCCGGCCACTGGTACCAATGGCCAACTGATGTCCAACGCTCTGCCTTCAGGACCAGG ACCTGAGCTGAGAGACAGCAGCCAACAGGCAGCACACATGAACCATCTCTCACACCCCTGGGGGCTGAAC CTCACCGAGAAAATCAAGAAGATTAAGGTCGTCTTCACTCCCACCATCTGCAAGCAGACCTGTGCCCGGG GCCGCTGTGCCAACACGTGTGAGAAGGGTGACACCACCACCCTGTACAGTCAGGGCGGCCATGGGCATGA CCCCAAGTCTGGCTTCCGTATCTATTTCTGCCAAATCCCCTGCCTGAATGGAGGCCGCTGCATTGGCCGG GACGAGTGCTGGTGTCCAGCCAACTCTACAGGGAAGTTCTGCCATCTGCCTGTCCCACAGCCAGACAGGG AGCCTCCAGGACGAGGCTCCCAGCACAGAGCCCTGCTGGAAGGGCCATTGAAGCAATCCACCTTCACGCT GCCTCTCTCCAACCAGCTGGCCTCTGTGAACCCCTCGCTGGTGAAGGTACAAATGCAGCACCCGCCTGAG GCCTCCGTGCAGATCCACCAGGTGGCCCGGGTCCGGGGTGAGGTGGACCCTGTGCCAGAGGACAACAGTG TGGAGACCAGAGCCTCTCATCGCCCCCATGGCAGCTCAGGCCACAGCCACTGGGCCAGCAACAGCATACC CGCTCGGGCTGGAGAGGCCCCTCGGCCACCACCAGTGCCGTCCAGGCATTATGGACTTCTGGGCCAGTGT TACCTGAGCACGGTGAATGGACAGTGTGCTAACCCCCTAGGGGAGCTGACTTCTCAGGAAGACTGCTGTG GCAGTGTGGGGACTTCTTGGGGGGTGACTTCCTGTGCCCCATGCCCACCCAGACCAGCTTTCCCCGTGAT TGAAAACGGCCAGCTGGAGTGTCCCCAAGGGTATAAGAGACTAAACCTCAGCCATTGCCAAGACATCAAT GAGTGCCTGACCCTGGGCCTGTGCAAGGATTCAGAGTGTGTGAACACCAGGGGCAGCTACCTGTGCACCT GCAGGCCCGGCCTCATGCTGGATCCATCAAGGAGCCGCTGTGTATCGGACAAGGCTGTCTCCATGAAACA GGGACTCTGTTACCGGTCAATGGTGTCTGGCACCTGCACCCTGCCTTTGGTACAACGGATCACCAAGCAG ATATGCTGTTGCAGCCGTGTGGGCAAAGCCTGGGGCAGCAAATGTGAACACTGCCCCCTGCCTGGCACAG AAGCCTTCAGGGAGATCTGCCCTGCTGGCCATGGCTACGCCTACTCAAGCTCAGACATCCGCCTGTCTAT GAGGAAAGCTGAGGAAGAGGAACTGGCTAGCCCCGTAAGGGAACAGAGACAGCAGAGCAGTGGACCCCCA CCTGGGGCAGCAGAAAGGCAGCCACTCCGGGCAGCCACTGCCACCTGGATTGAGGCTGAGACCCTCCCTG ACAAAGGTGACTCTCGGGCTATTCAGATTACAACCAGTGCTCCCCACCTACCTGCCCGGGTACCAGGGGA TGCCACTGGAAGACCAACGCCATCATTGCCTGGACAGGGCATTCCAGAGGGTCCAGCAGAAGAGCAGGTG ATCCCTTCCAGTGATGTCCTGGTGACGCACGGTCCCCCAGGCTTTGATCCATGTTTCGCTGGAGCCTCCA ACATCTGTGGCCCTGGGACCTGTGTGAAGCTCCCAAATGGATACAGATGTGTCTGCAGCCCTGGTTACCA GCTACACCCCAGCCAGGACTACTGTACTGATGACAACGAGTGTCTGAGGAACCCCTGTGAAGGAAGAGGG CGCTGTGTCAACAGTGTGGGCTCCTACTCCTGCCTCTGCTACCCAGGCTACACACTAGCCACCCTAGGAG ACACACAGGAGTGCCAAGATGTGGATGAGTGTGAGCAGCCGGGGGTGTGCAGCGGTGGACGATGCAGCAA CACTGAGGGCTCGTACCACTGCGAGTGTGATCAGGGCTACGTCATGGTCAGAAGAGGACACTGCCAAGAT ATCAACGAATGCCGTCACCCTGGTACCTGCCCTGATGGGAGATGCGTCAACTCCCCTGGCTCCTACACTT GTCTGGCCTGTGAGGAGGGCTACATAGGGCAGAGCGGGAACTGTGTAGATATGAATGAGTGTCTGACCCC CGGGATATGTGCCCATGGAAGGTGCATCAACATGGAAGGCTCCTTTAGATGCTCTTGTGAGCCAGGCTAT GAGCTCACCCCAGACAAGAAGGGCTGCCGAGATGTGGACGAGTGTGCCAGCCGAGCCTCATGCCCCACCG GCCTCTGCCTCAACACGGAGGGCTCCTTCACCTGCTCAGCCTGTCAGAGTGGGTACTGGGTGAACGAAGA TGGCACTGCCTGTGAAGACCTGGATGAATGTGCCTTCCCCGGAGTCTGCCCCACAGGCGTCTGCACCAAC ACTGTGGGCTCCTTCTCCTGCAAGGACTGCGACAGGGGCTTCCGGCCCAGCCCCCTGGGCAACAGCTGTG AAGATGTGGATGAGTGTGAAGGTCCCCAGAACAGCTGCCTGGGAGGCGAGTGCAAGAACACAGATGGTTC CTACCAGTGCCTCTGTCCCCAGGGCTTCCAGCTGGCCAATGGCACCGTGTGTGAGGATGTGGACGAATGT GTTGGGGAAGAACACTGCGCTCCTCATGGCGAATGCCTCAACAGCCCGGGGTCCTTCTTCTGTCTCTGTG CACCCGGCTTTGCTAGTGCTGAGGGGGGCACCAGATGCCAGGATGTTGATGAATGTGCAACCACAGAGCC GTGTCTGGGAGGACACTGTGTCAACACCGAGGGCTCCTTCAACTGTCTGTGTGAGACTGGCTTCCAGCCC GCCCCAGACAGTGGAGAGTGTGTGGACATAGATGAATGTGCAAATGATACTGTGTGTGGGAACCATGGCT TCTGTGACAATACGGATGGCTCCTTCCGCTGCCTGTGTGACCAGGGCTTCGAGACCTCACCCTCAGGCTG GGAGTGTGTTGATGTGAACGAGTGTGAGCTCATGCTGGCAGTGTGTGGGGATGCACTCTGCGAGAACGTG GAAGGCTCCTTCCTGTGCCTTTGTGCCAGTGACCTTGAGGAGTATGATGCAGAAGAAGGACACTGCCGTC CTCGGGTGGCTGGAGCTCAGAGAATCCCAGAGGTCCCAACAGAGGAGCAGGCTGCAGGCCTTACCGGCAT GGAGTGCTATGCTGAACACAATGGTGGTCCTCCATGCTCTCAAATCTTGGGCCAGAACTCCACACAGGCT GAGTGCTGCTCGACCCAGGGTGCCAGATGGGGGGAAACCTGTGATCCCTGCCCATCTGAGGACTCAGTTG AATTCAGTGAGCTGTGCCCCAGTGGTCAAGGTTACATCCCAGTGGAAGGGGCCTGGACATTTGGACAAGC CATGTATACAGATGCCGACGAGTGCATACTGTTTGGGCCTGCTCTCTGCCAGAATGGCCGATGCCTCAAC ACAGTGCCTGGCTACATTTGCCTGTGCAACCCTGGCTACCACTATGATGCCGTCAGCAGGAAGTGCCAGG ATCACAACGAATGCCAGGACTTGGCCTGTGAGAACGGCGAGTGTGTGAACACAGAAGGCTCCTTCCACTG CTTCTGCAGTCCCCCCCTCATCCTAGACCTCAGCGGACAGCGCTGTGTGAACAGTACCAGCAGCTCAGAG GACTTCCCTGACCATGACATCCACATGGACATCTGCTGGAAAAAAGTCACCAATGACGTGTGCAGCCAGC CCTTGCGTGGGCACCATACTACCTATACAGAGTGCTGCTGCCAAGACGGGGAGGCCTGGAGCCAGCAGTG TGCTCTGTGCCCCCCCAGGAGCTCTGAGGTCTATGCTCAGCTGTGCAATGTGGCTCGGATTGAGGCAGAG AGGGAAGCAGGGATCCACTTCCGGCCAGGATATGAGTATGGCCCTGGCCCAGATGATCTACCTGAAACCC TCTACGGCCCAGATGGAGCCCCTTTCTATAACTACCTGGGCCCTGAGGACACTGTTCCTGAGCCTCCCTT CTCCAACACAGCCAGTCATTTGGGAGACAACACACCCATCCTTGAGCCTCCTCTGCAGCCCTCTGAACTT CAGCCCCACTATATCCCCAGCCATTCAGAACCCCTGGCTTCCTTCGAAGGCCTTCAGGCTGAGGAATGTG GCATCCTGAATGGCTGTGAGAATGGCCGCTGTGTGCGTGTGCGCGAGGGCTACACTTGTGACTGCTTTGA AGGCTTCCAGCTGGATACAGCCCTCATGGCCTGTGTGGATGTGAATGAGTGTGAAGACCTGAACGGGCCT GCGGCACTCTGTGCGCATGGTCACTGCGAGAACACAGAGGGTTCCTATCGCTGCCACTGTTCCCCTGGTT ACGTGGCAGAGCCCGGGCCCCCACACTGTGCAGCCAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210089 representing NM_021586 Red=Cloning site Green=Tags(s) MESTSLRGLRCPQLCSHSGAMRAPTTVRCSGRIQRARWRGFLPLVLALLMGTSHAQRDSVGRYEPASRDA NRLWRPVGNHPAAAAAKVYSLFREPDAPVPGLSPSEWNQPGQGIPGRLAEAEARRPSRAQQLRRVQSPVQ TRRSNPRGQQPPAARTAHSVVRLATPQRPAAARRGRLTGRNVCGGQCCPGWTTSNSTNHCIKPVCQPPCQ NRGSCSRPQLCICRSGFRGARCEEVIPEEEFDPQNARPVPRRSVEGAPGPHRSSEARGSLVTRIQPLLPP LPPPPSRTLSQTRPLQQHAGLSRTVRRYPATGTNGQLMSNALPSGPGPELRDSSQQAAHMNHLSHPWGLN LTEKIKKIKVVFTPTICKQTCARGRCANTCEKGDTTTLYSQGGHGHDPKSGFRIYFCQIPCLNGGRCIGR DECWCPANSTGKFCHLPVPQPDREPPGRGSQHRALLEGPLKQSTFTLPLSNQLASVNPSLVKVQMQHPPE ASVQIHQVARVRGEVDPVPEDNSVETRASHRPHGSSGHSHWASNSIPARAGEAPRPPPVPSRHYGLLGQC YLSTVNGQCANPLGELTSQEDCCGSVGTSWGVTSCAPCPPRPAFPVIENGQLECPQGYKRLNLSHCQDIN ECLTLGLCKDSECVNTRGSYLCTCRPGLMLDPSRSRCVSDKAVSMKQGLCYRSMVSGTCTLPLVQRITKQ ICCCSRVGKAWGSKCEHCPLPGTEAFREICPAGHGYAYSSSDIRLSMRKAEEEELASPVREQRQQSSGPP PGAAERQPLRAATATWIEAETLPDKGDSRAIQITTSAPHLPARVPGDATGRPTPSLPGQGIPEGPAEEQV IPSSDVLVTHGPPGFDPCFAGASNICGPGTCVKLPNGYRCVCSPGYQLHPSQDYCTDDNECLRNPCEGRG RCVNSVGSYSCLCYPGYTLATLGDTQECQDVDECEQPGVCSGGRCSNTEGSYHCECDQGYVMVRRGHCQD INECRHPGTCPDGRCVNSPGSYTCLACEEGYIGQSGNCVDMNECLTPGICAHGRCINMEGSFRCSCEPGY ELTPDKKGCRDVDECASRASCPTGLCLNTEGSFTCSACQSGYWVNEDGTACEDLDECAFPGVCPTGVCTN TVGSFSCKDCDRGFRPSPLGNSCEDVDECEGPQNSCLGGECKNTDGSYQCLCPQGFQLANGTVCEDVDEC VGEEHCAPHGECLNSPGSFFCLCAPGFASAEGGTRCQDVDECATTEPCLGGHCVNTEGSFNCLCETGFQP APDSGECVDIDECANDTVCGNHGFCDNTDGSFRCLCDQGFETSPSGWECVDVNECELMLAVCGDALCENV EGSFLCLCASDLEEYDAEEGHCRPRVAGAQRIPEVPTEEQAAGLTGMECYAEHNGGPPCSQILGQNSTQA ECCSTQGARWGETCDPCPSEDSVEFSELCPSGQGYIPVEGAWTFGQAMYTDADECILFGPALCQNGRCLN TVPGYICLCNPGYHYDAVSRKCQDHNECQDLACENGECVNTEGSFHCFCSPPLILDLSGQRCVNSTSSSE DFPDHDIHMDICWKKVTNDVCSQPLRGHHTTYTECCCQDGEAWSQQCALCPPRSSEVYAQLCNVARIEAE REAGIHFRPGYEYGPGPDDLPETLYGPDGAPFYNYLGPEDTVPEPPFSNTASHLGDNTPILEPPLQPSEL QPHYIPSHSEPLASFEGLQAEECGILNGCENGRCVRVREGYTCDCFEGFQLDTALMACVDVNECEDLNGP AALCAHGHCENTEGSYRCHCSPGYVAEPGPPHCAAKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021586
ORF Size	5361 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021586.2, NP_067597.2
RefSeq Size	6468 bp
RefSeq ORF	5364 bp
Locus ID	59106
UniProt ID	O35806
MW	192.4 kDa
Gene Summary	induced by TGFbeta1 and also acts to further activate TGF-beta1 signaling [RGD, Feb 2006]

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