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Phactr1 (NM_214457) Rat Tagged ORF Clone
CAT＃: RR210072
- TrueORF®
Phactr1 (Myc-DDK-tagged ORF) - Rat phosphatase and actin regulator 1 (Phactr1), (10 ug)
"NM_214457" in other vectors (3)
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CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC112561 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210072 representing NM_214457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTATCCCAAAATGGATTATTTTCTGGACGTTGAGTCGGCTCACAGACTCCTGGATGTGGAGTCAG CACAGAGATTCTTCTATAGCCAAGGAGCTCAAGCTCGCCGGGCGACCCTGCTCCTGCCTCCCACATTAAT GGCGGCTTCTTCGGAGGATGACATAGACCGGCGGCCCATCAGAAGAGTCCGCTCCAAGAGCGACACGCCA TATCTCGCAGAGGCCAGGATCTCCTTTAACCTGGGGGCAGCTGAGGAAGTGGAAAGGCTGGCGGCCATGC GCTCTGACTCGTTGGTCCCAGGTACGCACACCCCGCCCATCCGGAGGAGGAGTAAGTTTGCCAACCTGGG AAGGATTTTCAAGCCTTGGAAATGGAGGAAGAAGAAAAGCGAAAAGTTCAAGCACACGTCAGCAGCTCTG GAAAGGAAGATATCGATGAGGCAGAGCAGAGAGGAGCTGATCAAGCGAGGGGTCTTGAAGGAGATCTACG ATAAAGATGGAGAGCTCTCCATATCAAATGAAGATGACTCCCTCACAAACGGCCAGTCCCTGAGCTCCAG CCAGCTCTCTTTGCCTGCTCTGTCGGAAATGGAGCCTGTCCCAATGCCCAGGGACCCCTGCTCATATGAG GTGCTCCAAGCTTCAGACATTATGGATGGACCAGATCCTGGCGCCCCCGTGAAATTGCCTTGTCTGCCAG TGAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCCTGATCTGCATGCCTGTAGGGGGCCCAGAGCTCTC CCTGGCATCCTACACAGCCCAGAAGAGCAGCCAGCAGGCTGTGGCCCAGCACCACCACACCGTCCTGCCA TCCCAGATGCAGCACCAGCTGCAGTATGGCAGCCACGGCCAGCATCTCCCGTCCTCCACCGGCACCTTAC CCATGCACCCCTCGGGCTGCAGGATGATCGATGAGCTGAACAAGACACTTGCTATGACCATGCAGAGGCT GGAAAGCTCCGAGCAACGCGTCCCCTGCTCCACTTCTTACCACAGCTCTGGTTTGCACTCAAGTGACGGC GTCACAAAAGCAGGACCCTTGGGCCTTCCGGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGACG ACAATAAGGAAAATGTGCCTCATGAACCCGACTATGAAGACTCGTCCTGCCTGTATGCACGGGAAGAGGA GGAAGAGGAGGAGGACGAAGATGACGACGCCTCCTTGTACACCAGCTCACTGGCCATGAAGGTCTGCAGG AAGGACTCCTTAGCCATCAAACTGAGCAACAGGCCCTCCAAGCGAGAGCTAGAGGAAAAGAACATCCTTC CCAGGCAGACGGATGAGGAACGGCTGGAACTCAGACAACAGATCGGCACCAAGCTCACCAGGCGGCTAAG CCAAAGACCCACTGCAGAGGAACTGGAGCAGAGGAACATTCTGAAACCTCGGAATGAACAAGAGGAGCAG GAGGAGAAGCGGGAAATAAAGAGGAGGCTGACTCGCAAGCTCAGCCAAAGACCCACGGTGGAAGAACTCC GGGAGAGGAAGATCCTTATTCGTTTCAGTGACTATGTGGAGGTGGCAGACGCCCAAGACTATGACCGAAG GGCGGACAAGCCATGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATTCGGAAGGAACTCAATGAGTTT AAAAGTACCGAAATGGAAGTCCACGAGTTAAGTAGACACTTAACCAGGTTTCATCGACCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR210072 representing NM_214457
Red=Cloning site Green=Tags(s) MDYPKMDYFLDVESAHRLLDVESAQRFFYSQGAQARRATLLLPPTLMAASSEDDIDRRPIRRVRSKSDTP YLAEARISFNLGAAEEVERLAAMRSDSLVPGTHTPPIRRRSKFANLGRIFKPWKWRKKKSEKFKHTSAAL ERKISMRQSREELIKRGVLKEIYDKDGELSISNEDDSLTNGQSLSSSQLSLPALSEMEPVPMPRDPCSYE VLQASDIMDGPDPGAPVKLPCLPVKLSPPLPPKKVLICMPVGGPELSLASYTAQKSSQQAVAQHHHTVLP SQMQHQLQYGSHGQHLPSSTGTLPMHPSGCRMIDELNKTLAMTMQRLESSEQRVPCSTSYHSSGLHSSDG VTKAGPLGLPEIRQVPTVVIECDDNKENVPHEPDYEDSSCLYAREEEEEEEDEDDDASLYTSSLAMKVCR KDSLAIKLSNRPSKRELEEKNILPRQTDEERLELRQQIGTKLTRRLSQRPTAEELEQRNILKPRNEQEEQ EEKREIKRRLTRKLSQRPTVEELRERKILIRFSDYVEVADAQDYDRRADKPWTRLTAADKAAIRKELNEF KSTEMEVHELSRHLTRFHRP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_214457 |
| ORF Size | 1740 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_214457.2, NP_999622.2 |
| RefSeq Size | 2078 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 306844 |
| UniProt ID | P62024 |
| MW | 66.2 kDa |
| Gene Summary | Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity (By similarity). Involved in the regulation of cortical neuron migration and dendrite arborization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210072 | Phactr1 (untagged ORF) - Rat phosphatase and actin regulator 1 (Phactr1), (10 ug) |
CNY 7,410.00 |
|
| RR210072L3 | Lenti ORF clone of Phactr1 (Myc-DDK-tagged ORF) - Rat phosphatase and actin regulator 1 (Phactr1), (10 ug) |
CNY 9,310.00 |
|
| RR210072L4 | Lenti ORF clone of Phactr1 (mGFP-tagged ORF) - Rat phosphatase and actin regulator 1 (Phactr1), (10 ug) |
CNY 10,170.00 |
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