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F2 (NM_022924) Rat Tagged ORF Clone
CAT＃: RR210046
- TrueORF®
F2 (Myc-DDK-tagged ORF) - Rat coagulation factor II (thrombin) (F2), (10 ug)
"NM_022924" in other vectors (3)
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CNY 7,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210046 representing NM_022924
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCACGTCCGCGGCCTGGGCCTACCTGGCTGCCTGGCTCTGGCTGCCCTGGCTAGCCTGGTACACA GTCAGCATGTGTTCCTGGCCCCTCAGCAAGCACTGTCGCTGCTCCAGCGGGTCCGCAGAGCCAACAGCGG CTTCCTGGAGGAGCTCCGAAAGGGCAACCTGGAGCGTGAGTGCGTGGAAGAGCAGTGCAGCTATGAGGAG GCTTTTGAGGCCCTGGAGTCTCCCCAAGACACGGATGTGTTCTGGGCCAAGTACACAGTGTGTGATTCAG TGAGGAAACCTCGAGAGACCTTCATGGACTGTCTAGAAGGTCGCTGTGCTATGGATCTGGGTCTGAACTA CCATGGGAATGTGAGTGTCACCCATACCGGCATCGAGTGTCAGCTGTGGCGAAGCCGCTACCCACACAGG CCTGACATCAACTCCACCACCCATCCTGGGGCTGACCTGAAGGAGAACTTCTGCCGAAATCCAGACAGCA GCACCTCAGGGCCCTGGTGCTACACCACAGACCCTACTGTGCGCAGAGAGGAATGCAGTATCCCTGTCTG TGGTCAGGAGGGCCGTACCACTGTGAAAATGACTCCTCGCTCAAGAGGTTCCAAGGAGAATCTGTCGCCT CCATTGGGAGAGTGCCTCCTCGAGCGTGGCCGGCTGTACCAGGGGAACTTAGCTGTGACAACACTTGGGT CCCCCTGCCTGGCCTGGGACAGTTTGCCAACCAAGACCCTGAGCAAGTACCAGAACTTCGACCCTGAGGT GAAACTCGTGCAAAACTTCTGCCGCAATCCAGACCGGGATGAGGAGGGCGCGTGGTGCTTTGTTGCGCAG CAGCCTGGTTTTGAATACTGCAGCCTCAACTACTGTGATGAAGCGGTGGGAGAGGAGAACCATGACGGGG ACGAGTCCATCGCAGGACGCACCACCGACGCGGAGTTCCACACCTTCTTCGATGAGAGGACTTTCGGCCT TGGGGAGGCAGACTGTGGCCTGCGGCCTTTGTTCGAGAAGAAGTCCTTGACAGACAAAACGGAAAAGGAG CTTCTTGATTCTTACATAGATGGGCGCATCGTGGAGGGCTGGGACGCTGAGAAGGGTATCGCCCCATGGC AGGTGATGCTTTTTCGGAAGAGTCCCCAAGAGCTGCTGTGTGGAGCCAGCCTTATCAGTGATCGATGGGT CCTCACTGCTGCCCACTGCATTCTGTACCCACCCTGGGACAAGAACTTCACTGAGAATGACCTCCTGGTG CGCATTGGCAAGCACTCCAGAACCAGATATGAGCGGAATGTTGAAAAGATCTCCATGCTGGAAAAGATCT ACATCCACCCCAGATATAACTGGCGGGAAAACCTAGACCGTGACATTGCTCTGCTCAAGCTAAAGAAGCC AGTGCCCTTCAGTGACTACATTCACCCCGTGTGCTTGCCAGACAAGCAGACAGTAACCAGTTTGCTCCAG GCTGGTTATAAAGGGCGGGTGACAGGCTGGGGCAACCTTCGGGAGACGTGGACAACTAACATCAACGAGA TACAGCCCAGCGTCCTGCAGGTGGTGAACCTGCCCATTGTAGAGCGACCAGTGTGCAAGGCCTCTACCCG GATACGCATTACTGACAACATGTTCTGTGCTGGCTTCAAGGTGAATGACACCAAGCGAGGAGATGCTTGT GAAGGTGACAGTGGGGGACCTTTTGTCATGAAGAGCCCCTACAACCACCGCTGGTACCAAATGGGTATTG TCTCCTGGGGTGAAGGCTGTGACCGGAATGGGAAATATGGCTTCTACACGCACGTGTTCCGTCTGAAAAG GTGGATGCAGAAAGTCATTGATCAGCATAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210046 representing NM_022924
Red=Cloning site Green=Tags(s) MSHVRGLGLPGCLALAALASLVHSQHVFLAPQQALSLLQRVRRANSGFLEELRKGNLERECVEEQCSYEE AFEALESPQDTDVFWAKYTVCDSVRKPRETFMDCLEGRCAMDLGLNYHGNVSVTHTGIECQLWRSRYPHR PDINSTTHPGADLKENFCRNPDSSTSGPWCYTTDPTVRREECSIPVCGQEGRTTVKMTPRSRGSKENLSP PLGECLLERGRLYQGNLAVTTLGSPCLAWDSLPTKTLSKYQNFDPEVKLVQNFCRNPDRDEEGAWCFVAQ QPGFEYCSLNYCDEAVGEENHDGDESIAGRTTDAEFHTFFDERTFGLGEADCGLRPLFEKKSLTDKTEKE LLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLV RIGKHSRTRYERNVEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLLQ AGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDAC EGDSGGPFVMKSPYNHRWYQMGIVSWGEGCDRNGKYGFYTHVFRLKRWMQKVIDQHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022924 |
| ORF Size | 1851 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022924.2, NP_075213.2 |
| RefSeq Size | 2005 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 29251 |
| MW | 70.4 kDa |
| Gene Summary | catalyzes the preferential cleavage of Arg-Gly; activates fibrinogen to fibrin and releases fibrinopeptides A and B; involved in blood coagulation and wound repair [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210046 | F2 (untagged ORF) - Rat coagulation factor II (thrombin) (F2), (10 ug) |
CNY 7,890.00 |
|
| RR210046L3 | Lenti ORF clone of F2 (Myc-DDK-tagged ORF) - Rat coagulation factor II (thrombin) (F2), (10 ug) |
CNY 9,790.00 |
|
| RR210046L4 | Lenti ORF clone of F2 (mGFP-tagged ORF) - Rat coagulation factor II (thrombin) (F2), (10 ug) |
CNY 10,640.00 |
