Esd (NM_001106051) Rat Tagged ORF Clone

CAT#: RR210004

  • TrueORF®

Esd (Myc-DDK-tagged ORF) - Rat esterase D/formylglutathione hydrolase (Esd), (10 ug)



  "NM_001106051" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR210004 representing NM_001106051
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACTAAAACAGATTTCCAGCAACAAGTGCTTTGGCGGGCTCCAGAAAGTTTTTGAACATAGCAGTG
TTGAGCTGAAATGTAAAATGAAATTTGCTATCTACCTACCACCCCAGGCTGAGAGTGCAAAGTGCCCTGC
ACTTTACTGGCTGTCTGGTTTAACTTGCACAGAACAAAATTTCATATCAAAGTCTGGCTGTCAACAAGCT
GCCTCAGAACATGGCCTTGTGGTCATTGCTCCTGATACCAGCCCCCGTGGCTGCAATATTAAAGGAGAAG
ATGACAGCTGGGACTTCGGCACTGGTGCGGGGTTTTTTGTGAACGCCACTGAGGATCCTTGGAACACTAA
CTACAGAATGTACTCTTACGTAACAGAGGAGCTTCCACAACTCATAAATGCCAATTTCCCAGTGGACCCA
CAGAGGATATCTATTTTTGGTCACTCCATGGGAGGTCACGGAGCTCTGATTTGCGCTTTGAAAAATCCTG
GAAAATACAGATCTGTATCTGCATTTGCTCCAATTTGCAACCCTGTGCTCTGTCCTTGGGGCAAAAAAGC
TTTTAATGGATATTTGGGACCAGATCAAAGTAAATGGAAGGCGTACGATGCGACCTGTCTCGTGAAGTCC
TACTCGGGTCCACAGATTGACATACTAATTGATCAGGGGAAAGATGACGAGTTCCTCTCGAATGGCCAGT
TACTCCCTGACAACTTCATCGCTGCCTGCACAGAAAAGAAAATCCCTGTTGTTTTTAGGCTACAAGAGGG
TTATGATCATAGCTACTACTTCATTGCAACCTTCATCACTGACCACATCAGGCATCATGCCAAGTACCTG
AATGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR210004 representing NM_001106051
Red=Cloning site Green=Tags(s)

MELKQISSNKCFGGLQKVFEHSSVELKCKMKFAIYLPPQAESAKCPALYWLSGLTCTEQNFISKSGCQQA
ASEHGLVVIAPDTSPRGCNIKGEDDSWDFGTGAGFFVNATEDPWNTNYRMYSYVTEELPQLINANFPVDP
QRISIFGHSMGGHGALICALKNPGKYRSVSAFAPICNPVLCPWGKKAFNGYLGPDQSKWKAYDATCLVKS
YSGPQIDILIDQGKDDEFLSNGQLLPDNFIAACTEKKIPVVFRLQEGYDHSYYFIATFITDHIRHHAKYL
NA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001106051
ORF Size 846 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001106051.3, NP_001099521.1
RefSeq Size 1072 bp
RefSeq ORF 849 bp
Locus ID 290401
UniProt ID B0BNE5
MW 31.4 kDa
Gene Summary Serine hydrolase involved in the detoxification of formaldehyde.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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