Cybrd1 (NM_001011954) Rat Tagged ORF Clone
CAT#: RR209967
- TrueORF®
Cybrd1 (Myc-DDK-tagged ORF) - Rat cytochrome b reductase 1 (Cybrd1), (10 ug)
"NM_001011954" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Dcytb |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209967 representing NM_001011954
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGAAGGCTACCGGGGCTTCCTGGGACTGCTGGTGTCCGCGCTGCTCGTGGGCTTCCTGTCGG TGATCTTCGTGCTCATCTGGGTCCTGCACTTCCGAGAGGGGCTTGGCTGGGACGGCGGCGCGCTCGAGTT TAACTGGCACCCGGTGCTCGCCGTGACCGGCTTCGTCTTCATCCAGGGCATCGCCATCATCGTGTACAGA TTGCCATGGACCTGGAAGTGCAGCAAGTTCTTGATGAAATCCATCCACGCCGGGTTAAACGCTGTGGCTG CCATCCTGGCCATCATCTCAGTCGTGGCCGTGTTTGATTATCACAATGTCCGAAAAATCCCCCACATGTA CAGTCTGCACAGCTGGGTTGGACTGACAGTTCTCATACTCTACATCCAACAGCTTGTTGTAGGCTTTTTC ATCTTTCTGCTTCCATGGGCTCCGCCCTCTCTCCGGGCAATCGTCATGCCCATACATGTGTATTCTGGAC TCCTCCTCTTTGGAACAGTGATTGCCACGGTCCTCATGGGAGTGACGGAAAAGCTGTTCTTTGTCCTGAA AAATCCTTCGTACCATTCATTCCCACCAGAAGGTGTTTTTACAAATACCCTGGGCCTTCTGATTCTGGTA TTTGGAGCCCTCATCTTTTGGATTGTCACCAGGCCACAATGGAAACGCCCTAGGGAACCAGGCTCTATCC CTCTTCAGCTAAATGGAGGCAACGCAGACGGAATGGAGGGCGCCATAGCAATAAGCTCCGCCCACAACAT GGACGCTGCAGACGCAGAGTTAAGCAGCGAGGGAGCAGCCCGGAAAAGAACCCTCGGCCTTGTTGACACT GGCCAGAGGTCCACCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209967 representing NM_001011954
Red=Cloning site Green=Tags(s) MAMEGYRGFLGLLVSALLVGFLSVIFVLIWVLHFREGLGWDGGALEFNWHPVLAVTGFVFIQGIAIIVYR LPWTWKCSKFLMKSIHAGLNAVAAILAIISVVAVFDYHNVRKIPHMYSLHSWVGLTVLILYIQQLVVGFF IFLLPWAPPSLRAIVMPIHVYSGLLLFGTVIATVLMGVTEKLFFVLKNPSYHSFPPEGVFTNTLGLLILV FGALIFWIVTRPQWKRPREPGSIPLQLNGGNADGMEGAIAISSAHNMDAADAELSSEGAARKRTLGLVDT GQRSTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001011954 |
ORF Size | 858 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001011954.1, NP_001011954.1 |
RefSeq Size | 3427 bp |
RefSeq ORF | 861 bp |
Locus ID | 295669 |
UniProt ID | Q5RKJ2 |
MW | 31.5 kDa |
Gene Summary | Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209967 | Cybrd1 (untagged ORF) - Rat cytochrome b reductase 1 (Cybrd1), (10 ug) |
CNY 3,990.00 |
|
RR209967L3 | Lenti ORF clone of Cybrd1 (Myc-DDK-tagged ORF) - Rat cytochrome b reductase 1 (Cybrd1), (10 ug) |
CNY 6,080.00 |
|
RR209967L4 | Lenti ORF clone of Cybrd1 (mGFP-tagged ORF) - Rat cytochrome b reductase 1 (Cybrd1), (10 ug) |
CNY 6,650.00 |