Mlh3 (NM_001108043) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209710 representing NM_001108043 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGGTGTCTGTCAGATGAAGTACAAGCCAAGTTGCGTTCTGGTTTAGCCCTAAGCTCCTTAGGCC AGTGTGTTGAGGAACTTACCCTTAACAGTATTGATGCTGAGGCAACATGTGTGGCCATCAGAGTGAATAT GGAAACCTTCCAAGTTCAAGTGATAGACAACGGACTTGGGATGGCGGGGGATGACGTGGAGAAGGTGGGG AACCGGTATTTTACTAGTAAATGCCACTCAGTACGGGACCTGGAGAACCCAACGTTTTACGGTTTCCGAG GCGAGGCCTTGGCAAGTATAGCTGACATGGCTAGTGCTGTGGAAATTTCATCCAAGAAAAGCACAACATT GAAGACCTTTGTGAAGATGTTTCAGAACGGAAAAGCCCTCGCCGCCCGTGAGGCGGACTTGACCAGACCG AGTGTGGGGACTACAGTGACGGTCTATAACCTGTTTTACCAGTTTCCTGTGCGGAGGAAAAGCATGGACC CTAGACTAGAGTTTGAGAAGGTTAGGCAGAGGGTGGAAGCCCTCTCACTTATGCACCCCTCCATTTCTTT CTCTTTGAGGAATGACGTTTCTGGTTCCATGATTCTTCAGCTCCCTAAAACCAAAGACATATGTTCTCGG TTTTGTCAAATTTATGGATTGGGCAAGTCCCAAAAGTTAAGAGAAATACATTATAAGTATAAGGAATTTG AGTTTAATGGCTACATCAGCTCTGAAGCACACTACAATAAGAATATGCAGTTTTTGTTTGTGAACAGAAG ACTAGTTTTAAGAACAAAGTTGCATAAACTTATTGACTTTTTATTAAGGAAAGAAAGTATTATATGCAGG CCAAAGAATGGCTCTGCCAGTAGGCAAATGAATTCAAGTCCTCGACACCGTTCTGCCTCAGAACTCCATG GGATATATGTAATCAACGTGCAGTGCCCGTTCTGTGAGTATGATGTCTGCATAGAGCCAGCCAAAACTCT GATTGAATTCCAGAACTGGGATACCGTGTTGATTTGTGTTCAGGAAGGAATAAAAAAGTTTTTAAAGCAA GAAAAATTATTTGTAGAATTATCAAGTGAAGATATTAAGGAATTTAATGAAGATAATGGTTTTAGTTTGA TTGGTACAACACTTCAGACACAGTCTACTCATGAGAAGTGTGACCAGAGCAGTTTCCAGGAAGCGTGTAA TAATATTTTGGATTCCTATGAAATGTTTAATTTGCAGTCAAAAGCTGTGAAAAGAATAGCTACTTTAGAA AATAGAACCACACAAAATTCTGGCGATTCAGGAGCTATCAGAAAAAAGACAGAGGACTCATACACTTATG AATCAGATGACCTGGGCCATAGAAAAACTGTAGAGTCTGCGTTATACAACAGTGACAGTGCTTACTTAGG ATCAAGGATGTCAGAAGAAGACATAGCCAAAACATCACACGCTGGAGAAAATGAGAAATGGAAAAAATCT TTGTTGGAAAATAGGACTTCAGAAAGGCCACATGAAACCAGTCCAAAAATGTTTTCAAGCCCCATTCAAA CTCATCATCCCCTTGAGGAGAGTGAGGCAGATCTAGAAATAGAGATGAAAAGTAGTACTGTTAATGTCAT GGCTGCCAACATTCCTCCAAATAGTGAAATTCAGAGTCAACTGGAGCAACTTGAAGATGCTCCTGAAGTG GAGTGCCAACCTCTGCCCTTTGAGACAACATTATTAAGAGCACAGGGTACTCTGAAAAAGAAGGAGAGAA AAAAGGAGCTCAGTAGCCATGGAAGAGTAAATGTTTTTAGTTATGGACAGGTTAAATTGTGCTCCACTGG CTTTATCACTCATGTGGTACAACGCGAACCCGCCAAGTCACCTGAAACAGAACAGTTGTTTAAAAATTAT GCTCGACCTGGTCCTGTAAGTGCCCAGGAAGCATTTGGAAATAGAACACACCATGCAAGTGAGACTCCAG ACAGCAGTAGTTTAACAAGCACTTTAAGTAAAGAATCCACTCAGCCGCCCCACAAAAGATTCTGCAGAAC AAATACAGGTTATGGGACAGAGAACAAACCTGTAGCAACAGATGACAACTTGGCCCTTTTTCAGGGAAGC CGTAAAGAATCACACACAGATTGCTTTTTGCCTGATACAACCTCCTTTCCGTGGTGTAGAAATGTTTCCG GTGGTTATAAGAAAAAAGGTAAAGTGGGTTCCTTCAAACACGTAGTCCGTAGGAAGCTAAGCTTGCGTTC ACAAATAGGGTCTTTAGAGAAGTTTAAGAGGCAATATGGGAAGGTTAACAATTCCCTAGCTGCAGAAGAG GACAATGACACTGACTTCAGGACCCATCTTGAGCCTCACAATGAACCCGATGTTCTTCTGAAAGATAAGA GCCTCTCAGATATGTCTGATGGTGGTGAGATCACTCCATCAGAGCACAGTGATGGGTGTCAACCATTACG TCCCATCCTGTACCCAGAGACACTTTCCAAAGAAGATCGCTTAGAACAGAGGCCTAGTTTGAGAGAAAGT CCTATCACTCTGGCGGAATTGCTCCACGGTAACAGAAAACCTTCTGAGGTTAAGAAGTCCACTGAAGCCC TGGCTTCTAAATTATCCAGACTCAAGGATTCTGAAAAAGAGATGCAAACCATGGAGATGGCAGGTCCTGT TAGTGAACGTCCAGACTCAGATCCCAGTTGGAAAGACAATAGCCAGTGCGCTAGGCTGGACTTAAATTTT TGTGAATTATATAAAAATAAACTTGAAAAAACAGAGAGTGAAGTGCTTCCAATGCCAGATTCTGCCCCAG AGGATGATTCCATCAATAAAAACAGCGTGCTGTGTTCTAACAGTGTAACAGATGACAGGGAGAAACCAGA AACTTCCTTGCTGTTACCCTGTAGTGATTCTAAAATCAGCAAAGATTCAGATGTTCTTATCAGAGTCTCA GAGCAACAGAGTGGAAGCCCTGACTCTGCCAGCAGGGAGGTAACGCGTCAGGTAGAAGATAGCGCCGGCA GCCAATGTGGAATCTGTTCCCAGAGCCATGGGTTTAAAGCAAGATCTTGTTCTGACAATGAAGAATCAAG TACGCACAGTATGGACTGGCAGCAGCATTTTGACGTAACCCTGGGAAGAACGGTTTACATCAACAGAATG ACCGGGCTTAGCACGTTTGTTGCTCCTACTGATGACATTCAGACTCCTTGTACTAAAGATCTGACAACCG TGGCTGTGGATGTCCTACTTGGGAATGGGGTCCAGTACAGGTGTCATCCTTTTAGAAGCGACCTTGTTCT TCCTTTCCTCCCAAGAGCTCAGGAAGAGAGGACCAGGCGGAGACACAACAGAGATGCTGTAGATGCTGCT GCTGTCAGTGAACCACTTCAGTCTTTGTTTTCAGAATGGAGCAATCCAGTGTTTGCCCGATACCCAGAGG TTGCTGTTGACGTCAGCAGTGGCCAGGCTGGGAGCTTAGCCGTTAAGATTCACAATGTTCTATATCCCTA TCGCTTCACCAAAGAGATGATCCACTCAGTGCAGGTTCTCCAGCAAGTGGATAACAAGTTTATCGCCTGC TTAATGAGCTCGAGGACGGACGGGAGTGGCCAAGCAGGTGGAAACCTGTTAGTCCTGGTGGACCAGCACG CTGCCCATGAGCGCGTTCGTTTGGAGCAGCTAATCACTGATTCCTATGAGAAACAAGCTCCACAAAGTGC TGGCCGGAAGAAATTACTGTCTTCCACAATAATTCCTCCACTAGCGATCACCGTGTCAGAGGAACAAAGG AGACTCTTACGGTCTTACCACAAACATTTGGAAGATCTGGGACTTGAGTTGCTCTTTCCAGATGCTAGTG ACTCTTCGATCCTGGTGGGAAAAGTGCCACTCTGCTTTGTAGAAAGAGAAGCTAATGAACTTCGAAGAGG ACGATCTCCTGTGACTAAGAGTATTGTGGAGGAATTGATTCGAGAACAGCTGGAGCTGCTCCAGACCACG GGAGGTATCCAAGGGACACTGCCGCTGACTGTCCAGAAGGTGTTGGCCTCCCAGGCCTGCCACGGGGCCA TTAAGTTTAATGATCGTCTGAGTCTGGAGGAGAGCTGTCGGCTTATCGAAGCTCTGTCCTTGTGCCAGCT GCCATTTCAGTGTGCTCATGGGCGACCTTCAATGCTGCCCTTAGCTGACCTGGACCACTTGGAGCAGGAA AAACAGGTTAAACCCAATCTTACTAAACTTCGCAAAATGGCTCATGCCTGGCATCTCTTTGGAAAAGCAG AAGGCTGTGATACAAAGCAGGAGCCACAACAGCCCGTACCTCCTTGTGAGCCACCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209710 representing NM_001108043 Red=Cloning site Green=Tags(s) MIRCLSDEVQAKLRSGLALSSLGQCVEELTLNSIDAEATCVAIRVNMETFQVQVIDNGLGMAGDDVEKVG NRYFTSKCHSVRDLENPTFYGFRGEALASIADMASAVEISSKKSTTLKTFVKMFQNGKALAAREADLTRP SVGTTVTVYNLFYQFPVRRKSMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMILQLPKTKDICSR FCQIYGLGKSQKLREIHYKYKEFEFNGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKESIICR PKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQNWDTVLICVQEGIKKFLKQ EKLFVELSSEDIKEFNEDNGFSLIGTTLQTQSTHEKCDQSSFQEACNNILDSYEMFNLQSKAVKRIATLE NRTTQNSGDSGAIRKKTEDSYTYESDDLGHRKTVESALYNSDSAYLGSRMSEEDIAKTSHAGENEKWKKS LLENRTSERPHETSPKMFSSPIQTHHPLEESEADLEIEMKSSTVNVMAANIPPNSEIQSQLEQLEDAPEV ECQPLPFETTLLRAQGTLKKKERKKELSSHGRVNVFSYGQVKLCSTGFITHVVQREPAKSPETEQLFKNY ARPGPVSAQEAFGNRTHHASETPDSSSLTSTLSKESTQPPHKRFCRTNTGYGTENKPVATDDNLALFQGS RKESHTDCFLPDTTSFPWCRNVSGGYKKKGKVGSFKHVVRRKLSLRSQIGSLEKFKRQYGKVNNSLAAEE DNDTDFRTHLEPHNEPDVLLKDKSLSDMSDGGEITPSEHSDGCQPLRPILYPETLSKEDRLEQRPSLRES PITLAELLHGNRKPSEVKKSTEALASKLSRLKDSEKEMQTMEMAGPVSERPDSDPSWKDNSQCARLDLNF CELYKNKLEKTESEVLPMPDSAPEDDSINKNSVLCSNSVTDDREKPETSLLLPCSDSKISKDSDVLIRVS EQQSGSPDSASREVTRQVEDSAGSQCGICSQSHGFKARSCSDNEESSTHSMDWQQHFDVTLGRTVYINRM TGLSTFVAPTDDIQTPCTKDLTTVAVDVLLGNGVQYRCHPFRSDLVLPFLPRAQEERTRRRHNRDAVDAA AVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAGSLAVKIHNVLYPYRFTKEMIHSVQVLQQVDNKFIAC LMSSRTDGSGQAGGNLLVLVDQHAAHERVRLEQLITDSYEKQAPQSAGRKKLLSSTIIPPLAITVSEEQR RLLRSYHKHLEDLGLELLFPDASDSSILVGKVPLCFVEREANELRRGRSPVTKSIVEELIREQLELLQTT GGIQGTLPLTVQKVLASQACHGAIKFNDRLSLEESCRLIEALSLCQLPFQCAHGRPSMLPLADLDHLEQE KQVKPNLTKLRKMAHAWHLFGKAEGCDTKQEPQQPVPPCEPP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108043
ORF Size	4326 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108043.1, NP_001101513.1
RefSeq Size	5424 bp
RefSeq ORF	4329 bp
Locus ID	314320
MW	161.9 kDa

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