Wdr33 (NM_001107398) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209701 representing NM_001107398 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACAGAAATTGGCTCTCCTCCACGATTTTTCCATATGCCGAGGTTCCAGCACCAGGCTCCCCGAC AGTTGTTCTATAAGCGGCCTGACTTTGCACAGCAGCAGGCAATGCAGCAGCTCACCTTTGACGGGAAGCG GATGAGAAAGGCCGTGAACCGGAAAACCATAGACTACAACCCCTCTGTCATTAAGTATTTGGAGAATAGG ATATGGCAAAGAGATCAGAGAGATATGCGGGCAATTCAGCCGGATGCAGGTTATTATAATGATCTGGTCC CACCTATAGGAATGTTGAATAATCCTATGAATGCAGTAACAACGAAATTTGTTAGGACATCAACAAACAA AGTAAAATGCCCAGTGTTTGTTGTGAGGTGGACCCCGGAAGGAAGACGGTTGGTCACTGGAGCTTCTAGT GGGGAGTTCACCTTGTGGAACGGGCTGACTTTCAACTTTGAAACCATATTGCAGGCTCATGATAGCCCTG TGAGGGCCATGACTTGGTCACATAATGACATGTGGATGTTGACAGCAGATCATGGAGGATATGTGAAATA TTGGCAATCCAACATGAACAACGTCAAGATGTTCCAGGCACATAAGGAGGCGATTAGAGAGGCCAGTTTC TCACCCACGGATAATAAATTTGCTACATGCTCTGATGACGGCACTGTTAGAATCTGGGATTTTCTTCGTT GCCATGAGGAAAGAATTCTCCGAGGTCATGGTGCTGATGTGAAGTGTGTAGATTGGCATCCAACCAAAGG GTTGGTTGTTTCAGGAAGTAAAGATAGCCAGCAGCCAATCAAGTTCTGGGACCCCAAGACTGGGCAGAGT CTTGCAACACTTCACGCCCATAAAAACACGGTGATGGAAGTGAAATTAAACCTCAACGGCAACTGGCTCC TCACGGCCTCTCGTGACCATCTCTGTAAGCTTTTCGATATCCGAAACCTGAAAGAAGAGCTTCAGGTATT CCGAGGTCACAAGAAGGAAGCCACAGCTGTAGCCTGGCATCCTGTCCACGAAGGACTTTTTGCCAGCGGA GGATCCGACGGCTCCTTGTTATTCTGGCACGTTGGGGTGGAGAAGGAAGTAGGTGGGATGGAGATGGCCC ACGAAGGGATGATCTGGAGTTTGGCTTGGCATCCTCTGGGACATATTCTCTGCTCGGGCTCAAATGACCA TACCAGCAAATTCTGGACTCGAAATCGGCCAGGTGACAAAATGCGAGATCGATATAATCTGAACCTTTTA CCTGGCATGTCAGAAGACGGAGTAGAGTATGATGACCTGGAACCTAATAGCCTGGCAGTGATTCCAGGGA TGGGAATCCCAGAACAACTGAAACTGGCCATGGAGCAGGAGCAGATGGGGAAAGATGAATCAAATGACAT TGAAATGACAATTCCAGGCTTGGATTGGGGAATGGAAGAAGTAATGCAAAAGGATCAGAAAAAAGTTCCT CAGAAGAAAGTCCCTTATGCAAAGCCCATTCCTGCTCAGTTCCAGCAGGCCTGGATGCAAAATAAAGTTC CAATTCCTGCTCCAAATGAGGTGCTGAATGACAGGAAAGAGGATATTAAGCTAGAAGAGAAGAAAAAAAC ACAAGCAGAAATCGAGCAAGAAATGGCCACGCTGCAGTATACCAACCCACAGCTTTTAGAGCAACTTAAA ATTGAAAGGCTTGCCCAGAAGCAGGCCGATCAGATCCAGCCTCCTCCCTCGTCTGGTACTCCTCTCCTCG GACCTCAGCCCTTTTCTGGTCAAGGCCCAATGTCTCAGATCCCTCAAGGTTTTCAGCAGCCTCACCCATC TCAGCAGATGCCATTGGTGGCTCAAATGGGGCCTCCAGGTCCACAGGGCCAGTTCAGAGCCCCTGGACCC CAGGGACAAATGGGACCACAAGGCCCTCCATTGCATCAAGGAGGTGGGGGGCCACAAGGATTCATGGGAC CACAAGGACCCCAGGGCCCTCCCCAGGGTCTGCCACGGCCTCAGGACATGCATGGTCCACAAGGAATGCA GAGGCACCCTGGACCTCATGGCCCTTTGGGACCTCAAGGGCCACCCGGACCTCAAGGTAGTTCTGGTCCT CAAGGTCATATGGGTCCTCAGGGTCCACCAGGACCTCAGGGTCACATTGGCCCCCAAGGCCCACCTGCTC CTCAGGGCCATATGGGCCCACAGGGTCCTCCTGGTACTCAAGGAATGCAGGGACCACCTGGTCCCAGAGG AATGCAAGGACCTCCTCATCCTCATGGGATACAGGGTGGGCCAACATCTCAAGGGATCCAGGGACCTCTG ATGGGACTGAATCCAAGAGGAATGCAGGGACCCCCAGGACCTCGGGAGAATCAGGGTCCTGCTCCTCAAG GGATAATGATTGGCCATCCACCTCAGGAGATGAGAGGACCACATCCTCCAAGTGGACTGCTGGGACATGG CCCTCAGGAAATGAGAGGTCCTCAGGAGATGCGGGGCATGCAGGGGCCTCCACCACAGGGGTCAATGTTG GGCCCTCCCCAGGAACTTCGAGGACCCTCAGGTTCACAGGGGCAGCAGGGGCCACCCCAGGGCTCTTTGG GACCTCCACCCCAGGGTGGCATGCAAGGACCCCCTGGACCTCAGGGACAACAGAACCCGGCAAGAGGGCC ACATCCATCTCAAGGGCCAATACCATTCCAGCAGCAGAAAGCACCTCTGCTAGGTGATGGGCCCCGGGCC CCCTTCAATCAGGAAGGACAGAGTACAGGTCCCCCACCCTTGATACCAGGCCTAGGGCAACAGGGAGCAC AAGGCCGCATTCCTCCTCTGAACCCTGGACAAGGACCCGGCCCTAACAAAGGTGACTCTCGAGGCCCTCC CAACCATCACCTGGGCCCCATGTCAGAGAGGCGGCATGAGCAGAGCGGTGGTCCTGAGCATGGTCCTGAC AGGGGACCTTTCCGGGGTGGCCAGGACTGCAGGGGTCCTCCTGACAGGCGTGGCTCTCACCCTGATTTCC CTGATGACTTCAGCAGACCAGATGACTTCCATCCTGACAAGCGCTTTGGCCACCGGTTACGGGAATTCGA GGGACGAGGAGGACCCTTACCACAAGAAGAAAAGTGGAGGCGAGGGGGCCCTGGACCTCCCTTTCCACCT GATCACAGGGAGTTCAATGAGGGGGACGGCCGGGGTGCAGCCCGAGGCCCTCCAGGGGCATGGGAAGGCC GCAGACCTGGAGACGAACGATTCCCCCGGGATCCTGATGACCCACGATTTCGAGGGCGCAGAGAAGAAAG CTTTAGAAGAGGTGCTCCCCCAAGACACGAAGGCCGTGCTCCTCCAAGAGGAAGGGACAACTTTCCTGGT CCTGATGACTTTGGTCCAGAAGAGGTTTTCGATGCTTCTGATGAAGCAGCCCGAGGACGAGATCTCAGAG GTCGAGGCCGTGGCACCCCTAGAGGGGGATCCAGGAAGTGCTTACTCCCCACTCCTGATGAGTTTCCTCG CTTTGAAGGAGGACGGAAGCCAGACTCCTGGGATGGAAACCGAGAGCCCGGACCAGGTCATGAACACTTT CGTGATGCTCCCCGTCCTGATCATCCCCCTCACGATGGCCATTCACCAGCTAGCAGAGAGCGCTCCTCTT CTCTCCAAGGCATGGACATGGCCTCCCTACCACCCCGGAAGCGCCCCTGGCATGATGGGTCGGGCACTTC TGAACACAGAGAGATGGAGGCCCAAGGAGGCCCCTCGGAGGACCGAGGAAGCAAAGGCCGAGGGGGCCCA GGACCTTCCCAGAGAGTTCCAAAATCCGGGCGTTCCAGCTCTTTGGACGGAGACCATCATGATGGATACC ACAGAGATGAACCTTTTGGTGGCCCTCCAGGCAGCAGCTCATCTTCTCGAGGGGGCCGGAGTGGCAGTAA CTGGGGTAGAGGGAGTAACATGAACTCTGGCCCTCCAAGGAGAGGCACTTCAAGAGGTAGTGGGAGGGGT CGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209701 representing NM_001107398 Red=Cloning site Green=Tags(s) MATEIGSPPRFFHMPRFQHQAPRQLFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIKYLENR IWQRDQRDMRAIQPDAGYYNDLVPPIGMLNNPMNAVTTKFVRTSTNKVKCPVFVVRWTPEGRRLVTGASS GEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHGGYVKYWQSNMNNVKMFQAHKEAIREASF SPTDNKFATCSDDGTVRIWDFLRCHEERILRGHGADVKCVDWHPTKGLVVSGSKDSQQPIKFWDPKTGQS LATLHAHKNTVMEVKLNLNGNWLLTASRDHLCKLFDIRNLKEELQVFRGHKKEATAVAWHPVHEGLFASG GSDGSLLFWHVGVEKEVGGMEMAHEGMIWSLAWHPLGHILCSGSNDHTSKFWTRNRPGDKMRDRYNLNLL PGMSEDGVEYDDLEPNSLAVIPGMGIPEQLKLAMEQEQMGKDESNDIEMTIPGLDWGMEEVMQKDQKKVP QKKVPYAKPIPAQFQQAWMQNKVPIPAPNEVLNDRKEDIKLEEKKKTQAEIEQEMATLQYTNPQLLEQLK IERLAQKQADQIQPPPSSGTPLLGPQPFSGQGPMSQIPQGFQQPHPSQQMPLVAQMGPPGPQGQFRAPGP QGQMGPQGPPLHQGGGGPQGFMGPQGPQGPPQGLPRPQDMHGPQGMQRHPGPHGPLGPQGPPGPQGSSGP QGHMGPQGPPGPQGHIGPQGPPAPQGHMGPQGPPGTQGMQGPPGPRGMQGPPHPHGIQGGPTSQGIQGPL MGLNPRGMQGPPGPRENQGPAPQGIMIGHPPQEMRGPHPPSGLLGHGPQEMRGPQEMRGMQGPPPQGSML GPPQELRGPSGSQGQQGPPQGSLGPPPQGGMQGPPGPQGQQNPARGPHPSQGPIPFQQQKAPLLGDGPRA PFNQEGQSTGPPPLIPGLGQQGAQGRIPPLNPGQGPGPNKGDSRGPPNHHLGPMSERRHEQSGGPEHGPD RGPFRGGQDCRGPPDRRGSHPDFPDDFSRPDDFHPDKRFGHRLREFEGRGGPLPQEEKWRRGGPGPPFPP DHREFNEGDGRGAARGPPGAWEGRRPGDERFPRDPDDPRFRGRREESFRRGAPPRHEGRAPPRGRDNFPG PDDFGPEEVFDASDEAARGRDLRGRGRGTPRGGSRKCLLPTPDEFPRFEGGRKPDSWDGNREPGPGHEHF RDAPRPDHPPHDGHSPASRERSSSLQGMDMASLPPRKRPWHDGSGTSEHREMEAQGGPSEDRGSKGRGGP GPSQRVPKSGRSSSLDGDHHDGYHRDEPFGGPPGSSSSSRGGRSGSNWGRGSNMNSGPPRRGTSRGSGRG R TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107398
ORF Size	3993 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107398.2, NP_001100868.2
RefSeq Size	4525 bp
RefSeq ORF	3996 bp
Locus ID	307524
MW	145.4 kDa

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