Fn1 (NM_019143) Rat Tagged ORF Clone
CAT#: RR209525
- TrueORF®
Fn1 (Myc-DDK-tagged ORF) - Rat fibronectin 1 (Fn1), (10 ug)
"NM_019143" in other vectors (1)
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CNY 47,690.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FIBNEC; fn-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209525 representing NM_019143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGGGTCCGGGACCCGGGCGGCTGCTGCTGCTAGCAGTCCTGTGCCTGGGGACATCGGTGCGCT GCACCGAAACCGGGAAGAGCAAGAGGCAGGCTCAGCAAATCGTGCAGCCTCCGTCCCCGGTGGCTGTCAG TCAGAGCAAGCCTGGCTGTTTTGACAACGGGAAGCATTATCAGATAAATCAGCAGTGGGAACGGACCTAC CTAGGCAACGCCCTGGTTTGTACCTGCTATGGAGGAAGCAGAGGTTTTAACTGCGAGAGCAAGCCTGAAC CTGAAGAGACCTGTTTTGACAAATACACTGGAAACACTTACAAAGTGGGTGACACTTATGAGCGCCCTAA AGATTCCATGATCTGGGACTGTACCTGCATTGGGGCTGGGCGAGGCAGGATCAGCTGTACCATTGCAAAT CGCTGCCATGAAGGGGGTCAGTCCTACAAGATTGGTGACAAGTGGAGGAGGCCACATGAGACTGGTGGCT ATATGTTGGAGTGTTTGTGTCTGGGGAATGGAAAAGGGGAATGGACCTGCAAGCCAATAGCTGAGAAATG TTTTGATCACGCTGCTGGGACTTCCTACGTCGTGGGGGAGACCTGGGAAAAGCCCTACCAAGGCTGGATG ATGGTGGACTGTACTTGTCTGGGCGAAGGCAATGGGCGTATCACCTGCACCTCCCGGAACAGATGCAATG ATCAGGACACCAGGACGTCCTACAGAATTGGAGACACATGGAGCAAGAAGGACAACAGAGGGAACCTGCT CCAGTGTGTCTGCACAGGCAACGGCAGAGGGGAGTGGAAGTGTGAGCGACATGTTCTACAGAGTGCTTCA GCTGGATCTGGCTCCTTCACAGATGTCCGAACAGCTATTTACCAACCCCAGACCCACCCCCAGCCCGCAC CGTACGGCCACTGTGTCACAGACAGCGGTGTGGTCTACTCTGTGGGAATGCAGTGGCTGAAGTCTCAAGG AGACAAGCAGATGCTGTGCACTTGCCTGGGCAATGGCGTCAGCTGCCAGGAGACAGCTGTGACCCAGACT TACGGTGGCAACTCAAACGGGGAGCCCTGTGTTCTCCCGTTCACCTACAACGGTAGGACCTTCTACTCCT GCACCACCGAAGGGCGGCAAGACGGACATCTGTGGTGTAGCACAACTTCAAATTATGAACAAGACCAGAA GTATTCTTTCTGCACAGACCACGCGGTTTTGGTTCAGACTCGAGGTGGGAATTCCAATGGTGCCTTGTGC CACTTCCCCTTCCTGTACAACAACCGGAATTACACCGACTGTACTTCTGAGGGTAGGCGGGACAACATGA AATGGTGCGGCACCACCCAGAACTACGATGCCGATCAGAAGTTTGGATTCTGCCCAATGGCTGCCCATGA GGAGATCTGCACGACCAACGAAGGGGTCATGTATCGCATTGGGGACCAGTGGGATAAGCAGCATGACCTG GGCCACATGATGAGGTGCACGTGTGTTGGGAACGGCCGTGGAGAATGGGCCTGCATCCCCTACTCCCAGC TCCGAGATCAGTGCATCGTTGATGACATTACTTACAACGTCAACGACACGTTCCACAAGCGTCACGAGGA GGGACATATGCTGAACTGTACCTGCTTCGGTCAGGGCCGGGGCAGATGGAAATGTGACCCCATCGACCAG TGCCAAGATTCAGAGACCCGGACATTTTACCAGATTGGTGACTCCTGGGAGAAGTTTGTGCATGGTGTCA GATACCAGTGTTACTGTTACGGCCGTGGCATTGGGGAGTGGCACTGCCAGCCTCTGCAGACCTACCCAGG CACAACTGGACCTGTTCAAGTAATTATCACGGAGACCCCCAGCCAGCCCAATTCCCACCCCATCCAGTGG AATGCCCCGGAGCCTTCACACATCACCAAGTACATTCTCAGGTGGAGACCTAAAACCTCTACGGGTCGCT GGAAGGAAGCTACCATTCCAGGCCACCTTAACTCCTATACCATCAAAGGCCTGACCCCAGGTGTGATCTA CGAGGGACAGCTCATCAGCATCCAGCAGTACGGGCACCAAGAAGTGACTCGCTTTGACTTCACCACCAGC GCCAGCACACCTGTGACCAGCAACACAGTGACTGGAGAGACTGCGCCCTTTTCTCCTGTTGTGGCCACTT CCGAATCTGTCACTGAAATCACAGCCAGCAGCTTCGTGGTCTCCTGGGTCTCAGCTTCCGACACGGTGTC AGGATTCCGAGTGGAGTACGAACTGAGCGAGGAAGGAGATGAGCCTCAGTACCTTGATCTTCCAAGCACA GCCACTTCTGTGAACATTCCTGACCTGCTCCCGGGCAGAAAGTACATCGTCAACGTCTATCAGATATCTG AAGAGGGAAAGCAGAGCTTGATCCTGTCTACATCACAGACTACAGCACCTGATGCGCCTCCAGACCCTAC TGTGGACCAGGTTGATGACACTTCCATTGTTGTTCGATGGAGCAGACCCCAGGCACCTATCACAGGGTAC AGGATTGTCTATTCACCTTCAGTAGAAGGCAGTAGCACAGAACTCAACCTTCCTGAAACGGCCAACTCCG TCACCCTCAGCGACCTGCAGCCCGGTGTTCAGTACAACATCACTATCTATGCTGTGGAGGAGAACCAGGA GAGCACACCCGTTTTCATCCAGCAGGAGACTACTGGCGTCCCACGATCCGATGATGTTCCCGCTCCAAAG GACCTACAGTTTGTGGAAGTGACCGACGTGAAAGTCACCATCATGTGGACACCTCCTAATAGCGCAGTGA CTGGATACCGTGTGGATGTCCTGCCTGTCAACCTGCCAGGGGAACATGGGCAGAGGCTGCCTGTCAACAG GAACACCTTTGCTGAAGTCACCGGACTGTCCCCAGGGGTCACGTACCTCTTCAAAGTCTTTGCTGTGCAT CAGGGCAGGGAAAGCAAGCCTCTGACAGCACAACAGACCACCAAACTCGATGCTCCCACTAACCTCCAGT TTGTCAATGAAACGGACAGAACAGTTCTGGTAACTTGGACTCCACCTCGAGCCCGGATAGCAGGCTACCG ACTGACAGTGGGCCTCACCCGAGGAGGCCAGCCCAAGCAGTACAATGTGGGACCCATGGCTTCCAAGTAT CCCCTGAGAAATCTGCAGCCTGGGTCTGAGTACACTGTGACCTTGATGGCTGTGAAAGGCAACCAGCAGA GTCCCAAAGCCACCGGAGTCTTTACTACCCTGCAGCCTCTGCGCTCCATTCCACCTTATAACACCGAGGT GACAGAGACCACAATCGTGATCACCTGGACCCCCGCTCCAAGGATTGGCTTCAAGCTGGGTGTACGACCA AGCCAGGGAGGTGAAGCACCCCGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCTGGCTTGACTC CAGGCGTGGAATACACGTACACCATCCAAGTCCTGAGGGACGGCCAGGAGAGAGATGCACCAATTGTCAA CCGAGTAGTGACACCGCTGTCTCCCCCAACCAACTTGCACCTGGAGGCCAATCCTGACACTGGAGTGCTT ACCGTCTCCTGGGAGAGGAGCACCACCCCAGATATTACTGGCTACAGAATAACCACCACCCCCACAAACG GGCAGCAGGGAACCGCTTTGGAAGAAGTGGTTCATGCCGATCAGAGTTCCTGCACTTTTGAAAACCTGAA TCCTGGCCTGGAGTACAATGTCAGTGTTTACACTGTCAAAGATGACAAGGAAAGTGCCCCTATCTCTGAT ACCGTCATCCCAGAGGTGCCCCAGCTCACTGACCTAAGCTTTGTTGATATAACTGACTCAAGCATCGGCC TGAGGTGGACCCCGCTAAACTCTTCCACCATTATCGGGTACCGAATCACAGTAGTTGCGGCAGGAGAAGG GATCCCCATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTTACAGGGCTGGAACCCGGC ATTGACTATGACATCAGCGTTATCACTCTCATTAATGGCGGAGAGAGTGCCCCTACTACACTGACACAGC AAACGGCCGTCCCTCCTCCCACGGATCTGCGATTCACCAATATCGGTCCGGACACTATGCGGGTCACTTG GGCCCCGCCTCCGTCCATTGAGCTAACCAACCTCTTGGTGCGCTACTCACCTGTGAAGAACGAGGAGGAT GTGGCAGAGCTGTCCATTTCACCCTCAGACAACGCCGTGGTCCTAACAAATCTCCTGCCTGGGACTGAGT ACTTAGTCAGTGTCTCCAGCGTGTACGAACAGCATGAGAGCATCCCTCTCAGAGGAAGACAGAAAACAGG TCTGGACTCCCCAACTGGTTTTGATTCTTCTGATGTCACCGCCAACTCATTCACCGTCCACTGGGTGGCT CCTCGGGCCCCCATCACCGGCTACATCATCCGCCATCACGCCGAGCATTCTGCCGGAAGACCCAGGCAAG ACCGAGTGCCGCCCTCAAGGAATTCTATCACCCTCACCAACCTTAATCCGGGCACGGAGTACATTGTCAC CATCATTGCTGTTAATGGCAGAGAGGAGAGCCCCCCACTGATTGGCCAGCAATCCACGGTTTCCGATGTC CCGAGAGATCTGGAGGTCATCGCTTCCACCCCCACCAGCCTGCTCATCAGTTGGGAACCCCCCGCCGTCT CTGTGCGCTATTACAGAATCACCTATGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAATTCACTGTGCC CGGAAGCAAGTCCACCGCCACCATCAACAACATTAAACCAGGAGCAGACTACACCATCACCCTGTATGCT GTCACTGGCCGTGGGGACAGTCCAGCCAGCAGCAAGCCAGTTTCCATCAATTATCAAACAGAAATTGACA AGCCATCCCAGATGCAGGTGACGGATGTCCAGGACAACAGCATCAGTGTCAGGTGGCTGCCTTCAACTTC TCCTGTGACAGGTTACAGAGTGACCACCGCTCCCAAAAATGGCCTAGGACCAACAAAATCTCAAACTGTC AGTCCAGATCAAACAGAAATGACCATTGAAGGTTTGCAACCCACCGTGGAGTATGTGGTTAGTGTCTATG CTCAGAACCGGAACGGAGAAAGCCAGCCCCTGGTTCAGACTGCAGTGACCAACATTGACCGCCCTAAAGG ACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCCTGGGAAAGCCCACAGGGGCAAGTTTCC AGGTACAGGGTGACCTACTCAAGCCCTGAGGATGGAATCCATGAGCTTTTCCCTGCGCCTGATGGTGACG AGGACACGGCAGAGCTGCACGGCCTCAGGCCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGG TGGCATGGAGAGCCAGCCCCTGATTGGAGTCCAGTCCACAGCCATTCCTGCGCCAACCAATCTGAAGTTC ACTCAGGTGTCACCCACCACCTTGACTGCCCAGTGGACAGCACCCAGTGTTAAGCTCACTGGCTACCGAG TGCGGGTGACCCCGAAGGAGAAGACAGGACCAATGAAAGAAATCAACCTTTCTCCAGACAGCACCTCCGT GATTGTGTCAGGGCTCATGGTGGCCACCAAGTATGAAGTCAGCGTCTATGCTCTCAAGGACACATTGACA AGCAGACCAGCTCAGGGAGTCGTCACGACTCTGGAGAATGTCAGCCCTCCAAGAAGGGCCCGTGTGACCG ACGCTACAGAAACTACCATCACTATTAGCTGGAGAACGAAGACAGAGACGATCACTGGCTTCCAAGTCGA TGCCATTCCAGCCAATGGCCAGACCCCGGTTCAGAGGACCATCAGCCCGGATGTCAGAAGCTATACTATT ACAGGTTTACAGCCAGGCACTGACTACAAGATCCACCTGTACACGCTCAACGACAATGCCCGGAGCTCTC CTGTGGTCATTGATGCCTCCACGGCCATTGATGCCCCATCCAACCTGCGGTTCCTGACCACCACACCCAA CTCCTTGCTGGTATCATGGCAGGCACCCCGTGCCAGGATTACTGGCTACATTATCAAGTATGAGAAGCCT GGATCCCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACGGAGGCCACCATCACTGGTCTGG AGCCAGGAACCGAGTACACCATCTATGTCATCGCACTGAAGAACAATCAGAAGAGTGAGCCCCTGATTGG GAGGAAAAAGACAGATGAGCTTCCCCAACTGGTTACCCTTCCACACCCCAATCTTCATGGACCAGAGATC TTGGATGTTCCCTCCACAGTTCAAAAGACCCCCTTCGTCACCAACCCTGGGTATGACACCGAAAATGGTA TTCAGCTTCCTGGCACATCCCACCAACAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGCTT TAGGCGAACCACGCCACCCACTGCGGCCACCCCCGTCAGGCTTAGGCCAAGACCATACCTGCCGAATGTA GATGAGGAGGTCCAAATCGGTCATGTTCCCAGGGGAGACGTAGACTACCACCTCTATCCTCATGTTCCGG GGCTCAATCCAAATGCCTCTACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCTTGGACGCCATTCCA GGAGAGTTCTGAGTACATCATTTCATGCCAACCTGTTGGCACTGACGAAGAGCCCTTACAGTTCCAAGTT CCTGGAACTTCTACCAGTGCGACTCTGACTGGCCTTACCAGAGGGGTCACCTACAACATCATAGTGGAGG CCCTGCACAACCAGAGGAGGCACAAGGTCCGAGAAGAGGTTGTTACTGTAGGCAACACTGTCAACGAAGG CCTGAACCAGCCTACGGATGACTCATGCTTTGACCCTTACACGGTTTCCCATTACGCCGTTGGAGAGGAA TGGGAGCGGTTATCTGACTCTGGCTTTAAGCTCACTTGCCAGTGCTTGGGCTTTGGCAGTGGTCATTTCA GATGCGATTCATCTAAATGGTGCCATGACAACGGTGTCAACTACAAGATCGGAGAGAAGTGGGATCGTCA GGGAGAAAATGGCCAGCGGATGAGCTGCACATGTCTCGGGAATGGAAAGGGAGAATTCAAATGCGATCCC CATGAAGCAACGTGTTATGACGACGGGAAGACCTACCACGTAGGAGAACAGTGGCAGAAAGAGTATCTCG GAGCCATTTGCTCCTGCACGTGTTTCGGGGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGG GGCTGCTGAACCCAGTCCCGATGGTACCACTGGCCACACCTACAACCAGTATACACAGAGATACCATCAG AGAACGAACACTAATGTAAATTGCCCAATTGAATGCTTCATGCCGTTGGACGTGCAGGCTGACAGAGATG ATTCCAGAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209525 representing NM_019143
Red=Cloning site Green=Tags(s) MLRGPGPGRLLLLAVLCLGTSVRCTETGKSKRQAQQIVQPPSPVAVSQSKPGCFDNGKHYQINQQWERTY LGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYKVGDTYERPKDSMIWDCTCIGAGRGRISCTIAN RCHEGGQSYKIGDKWRRPHETGGYMLECLCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWM MVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCVCTGNGRGEWKCERHVLQSAS AGSGSFTDVRTAIYQPQTHPQPAPYGHCVTDSGVVYSVGMQWLKSQGDKQMLCTCLGNGVSCQETAVTQT YGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHAVLVQTRGGNSNGALC HFPFLYNNRNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDL GHMMRCTCVGNGRGEWACIPYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQ CQDSETRTFYQIGDSWEKFVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQW NAPEPSHITKYILRWRPKTSTGRWKEATIPGHLNSYTIKGLTPGVIYEGQLISIQQYGHQEVTRFDFTTS ASTPVTSNTVTGETAPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPST ATSVNIPDLLPGRKYIVNVYQISEEGKQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGY RIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRSDDVPAPK DLQFVEVTDVKVTIMWTPPNSAVTGYRVDVLPVNLPGEHGQRLPVNRNTFAEVTGLSPGVTYLFKVFAVH QGRESKPLTAQQTTKLDAPTNLQFVNETDRTVLVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPMASKY PLRNLQPGSEYTVTLMAVKGNQQSPKATGVFTTLQPLRSIPPYNTEVTETTIVITWTPAPRIGFKLGVRP SQGGEAPREVTSDSGSIVVSGLTPGVEYTYTIQVLRDGQERDAPIVNRVVTPLSPPTNLHLEANPDTGVL TVSWERSTTPDITGYRITTTPTNGQQGTALEEVVHADQSSCTFENLNPGLEYNVSVYTVKDDKESAPISD TVIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPG IDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIELTNLLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESIPLRGRQKTGLDSPTGFDSSDVTANSFTVHWVA PRAPITGYIIRHHAEHSAGRPRQDRVPPSRNSITLTNLNPGTEYIVTIIAVNGREESPPLIGQQSTVSDV PRDLEVIASTPTSLLISWEPPAVSVRYYRITYGETGGNSPVQEFTVPGSKSTATINNIKPGADYTITLYA VTGRGDSPASSKPVSINYQTEIDKPSQMQVTDVQDNSISVRWLPSTSPVTGYRVTTAPKNGLGPTKSQTV SPDQTEMTIEGLQPTVEYVVSVYAQNRNGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVS RYRVTYSSPEDGIHELFPAPDGDEDTAELHGLRPGSEYTVSVVALHGGMESQPLIGVQSTAIPAPTNLKF TQVSPTTLTAQWTAPSVKLTGYRVRVTPKEKTGPMKEINLSPDSTSVIVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAIPANGQTPVQRTISPDVRSYTI TGLQPGTDYKIHLYTLNDNARSSPVVIDASTAIDAPSNLRFLTTTPNSLLVSWQAPRARITGYIIKYEKP GSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEI LDVPSTVQKTPFVTNPGYDTENGIQLPGTSHQQPSVGQQMIFEEHGFRRTTPPTAATPVRLRPRPYLPNV DEEVQIGHVPRGDVDYHLYPHVPGLNPNASTGQEALSQTTISWTPFQESSEYIISCQPVGTDEEPLQFQV PGTSTSATLTGLTRGVTYNIIVEALHNQRRHKVREEVVTVGNTVNEGLNQPTDDSCFDPYTVSHYAVGEE WERLSDSGFKLTCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQRMSCTCLGNGKGEFKCDP HEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAAEPSPDGTTGHTYNQYTQRYHQ RTNTNVNCPIECFMPLDVQADRDDSRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019143 |
ORF Size | 7431 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019143.2, NP_062016.2 |
RefSeq Size | 8331 bp |
RefSeq ORF | 7434 bp |
Locus ID | 25661 |
MW | 272.4 kDa |
Gene Summary | extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209525 | Fn1 (untagged ORF) - Rat fibronectin 1 (Fn1), (10 ug) |
CNY 47,690.00 |