Fn1 (NM_019143) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FIBNEC; fn-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209525 representing NM_019143 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGGGTCCGGGACCCGGGCGGCTGCTGCTGCTAGCAGTCCTGTGCCTGGGGACATCGGTGCGCT GCACCGAAACCGGGAAGAGCAAGAGGCAGGCTCAGCAAATCGTGCAGCCTCCGTCCCCGGTGGCTGTCAG TCAGAGCAAGCCTGGCTGTTTTGACAACGGGAAGCATTATCAGATAAATCAGCAGTGGGAACGGACCTAC CTAGGCAACGCCCTGGTTTGTACCTGCTATGGAGGAAGCAGAGGTTTTAACTGCGAGAGCAAGCCTGAAC CTGAAGAGACCTGTTTTGACAAATACACTGGAAACACTTACAAAGTGGGTGACACTTATGAGCGCCCTAA AGATTCCATGATCTGGGACTGTACCTGCATTGGGGCTGGGCGAGGCAGGATCAGCTGTACCATTGCAAAT CGCTGCCATGAAGGGGGTCAGTCCTACAAGATTGGTGACAAGTGGAGGAGGCCACATGAGACTGGTGGCT ATATGTTGGAGTGTTTGTGTCTGGGGAATGGAAAAGGGGAATGGACCTGCAAGCCAATAGCTGAGAAATG TTTTGATCACGCTGCTGGGACTTCCTACGTCGTGGGGGAGACCTGGGAAAAGCCCTACCAAGGCTGGATG ATGGTGGACTGTACTTGTCTGGGCGAAGGCAATGGGCGTATCACCTGCACCTCCCGGAACAGATGCAATG ATCAGGACACCAGGACGTCCTACAGAATTGGAGACACATGGAGCAAGAAGGACAACAGAGGGAACCTGCT CCAGTGTGTCTGCACAGGCAACGGCAGAGGGGAGTGGAAGTGTGAGCGACATGTTCTACAGAGTGCTTCA GCTGGATCTGGCTCCTTCACAGATGTCCGAACAGCTATTTACCAACCCCAGACCCACCCCCAGCCCGCAC CGTACGGCCACTGTGTCACAGACAGCGGTGTGGTCTACTCTGTGGGAATGCAGTGGCTGAAGTCTCAAGG AGACAAGCAGATGCTGTGCACTTGCCTGGGCAATGGCGTCAGCTGCCAGGAGACAGCTGTGACCCAGACT TACGGTGGCAACTCAAACGGGGAGCCCTGTGTTCTCCCGTTCACCTACAACGGTAGGACCTTCTACTCCT GCACCACCGAAGGGCGGCAAGACGGACATCTGTGGTGTAGCACAACTTCAAATTATGAACAAGACCAGAA GTATTCTTTCTGCACAGACCACGCGGTTTTGGTTCAGACTCGAGGTGGGAATTCCAATGGTGCCTTGTGC CACTTCCCCTTCCTGTACAACAACCGGAATTACACCGACTGTACTTCTGAGGGTAGGCGGGACAACATGA AATGGTGCGGCACCACCCAGAACTACGATGCCGATCAGAAGTTTGGATTCTGCCCAATGGCTGCCCATGA GGAGATCTGCACGACCAACGAAGGGGTCATGTATCGCATTGGGGACCAGTGGGATAAGCAGCATGACCTG GGCCACATGATGAGGTGCACGTGTGTTGGGAACGGCCGTGGAGAATGGGCCTGCATCCCCTACTCCCAGC TCCGAGATCAGTGCATCGTTGATGACATTACTTACAACGTCAACGACACGTTCCACAAGCGTCACGAGGA GGGACATATGCTGAACTGTACCTGCTTCGGTCAGGGCCGGGGCAGATGGAAATGTGACCCCATCGACCAG TGCCAAGATTCAGAGACCCGGACATTTTACCAGATTGGTGACTCCTGGGAGAAGTTTGTGCATGGTGTCA GATACCAGTGTTACTGTTACGGCCGTGGCATTGGGGAGTGGCACTGCCAGCCTCTGCAGACCTACCCAGG CACAACTGGACCTGTTCAAGTAATTATCACGGAGACCCCCAGCCAGCCCAATTCCCACCCCATCCAGTGG AATGCCCCGGAGCCTTCACACATCACCAAGTACATTCTCAGGTGGAGACCTAAAACCTCTACGGGTCGCT GGAAGGAAGCTACCATTCCAGGCCACCTTAACTCCTATACCATCAAAGGCCTGACCCCAGGTGTGATCTA CGAGGGACAGCTCATCAGCATCCAGCAGTACGGGCACCAAGAAGTGACTCGCTTTGACTTCACCACCAGC GCCAGCACACCTGTGACCAGCAACACAGTGACTGGAGAGACTGCGCCCTTTTCTCCTGTTGTGGCCACTT CCGAATCTGTCACTGAAATCACAGCCAGCAGCTTCGTGGTCTCCTGGGTCTCAGCTTCCGACACGGTGTC AGGATTCCGAGTGGAGTACGAACTGAGCGAGGAAGGAGATGAGCCTCAGTACCTTGATCTTCCAAGCACA GCCACTTCTGTGAACATTCCTGACCTGCTCCCGGGCAGAAAGTACATCGTCAACGTCTATCAGATATCTG AAGAGGGAAAGCAGAGCTTGATCCTGTCTACATCACAGACTACAGCACCTGATGCGCCTCCAGACCCTAC TGTGGACCAGGTTGATGACACTTCCATTGTTGTTCGATGGAGCAGACCCCAGGCACCTATCACAGGGTAC AGGATTGTCTATTCACCTTCAGTAGAAGGCAGTAGCACAGAACTCAACCTTCCTGAAACGGCCAACTCCG TCACCCTCAGCGACCTGCAGCCCGGTGTTCAGTACAACATCACTATCTATGCTGTGGAGGAGAACCAGGA GAGCACACCCGTTTTCATCCAGCAGGAGACTACTGGCGTCCCACGATCCGATGATGTTCCCGCTCCAAAG GACCTACAGTTTGTGGAAGTGACCGACGTGAAAGTCACCATCATGTGGACACCTCCTAATAGCGCAGTGA CTGGATACCGTGTGGATGTCCTGCCTGTCAACCTGCCAGGGGAACATGGGCAGAGGCTGCCTGTCAACAG GAACACCTTTGCTGAAGTCACCGGACTGTCCCCAGGGGTCACGTACCTCTTCAAAGTCTTTGCTGTGCAT CAGGGCAGGGAAAGCAAGCCTCTGACAGCACAACAGACCACCAAACTCGATGCTCCCACTAACCTCCAGT TTGTCAATGAAACGGACAGAACAGTTCTGGTAACTTGGACTCCACCTCGAGCCCGGATAGCAGGCTACCG ACTGACAGTGGGCCTCACCCGAGGAGGCCAGCCCAAGCAGTACAATGTGGGACCCATGGCTTCCAAGTAT CCCCTGAGAAATCTGCAGCCTGGGTCTGAGTACACTGTGACCTTGATGGCTGTGAAAGGCAACCAGCAGA GTCCCAAAGCCACCGGAGTCTTTACTACCCTGCAGCCTCTGCGCTCCATTCCACCTTATAACACCGAGGT GACAGAGACCACAATCGTGATCACCTGGACCCCCGCTCCAAGGATTGGCTTCAAGCTGGGTGTACGACCA AGCCAGGGAGGTGAAGCACCCCGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCTGGCTTGACTC CAGGCGTGGAATACACGTACACCATCCAAGTCCTGAGGGACGGCCAGGAGAGAGATGCACCAATTGTCAA CCGAGTAGTGACACCGCTGTCTCCCCCAACCAACTTGCACCTGGAGGCCAATCCTGACACTGGAGTGCTT ACCGTCTCCTGGGAGAGGAGCACCACCCCAGATATTACTGGCTACAGAATAACCACCACCCCCACAAACG GGCAGCAGGGAACCGCTTTGGAAGAAGTGGTTCATGCCGATCAGAGTTCCTGCACTTTTGAAAACCTGAA TCCTGGCCTGGAGTACAATGTCAGTGTTTACACTGTCAAAGATGACAAGGAAAGTGCCCCTATCTCTGAT ACCGTCATCCCAGAGGTGCCCCAGCTCACTGACCTAAGCTTTGTTGATATAACTGACTCAAGCATCGGCC TGAGGTGGACCCCGCTAAACTCTTCCACCATTATCGGGTACCGAATCACAGTAGTTGCGGCAGGAGAAGG GATCCCCATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTTACAGGGCTGGAACCCGGC ATTGACTATGACATCAGCGTTATCACTCTCATTAATGGCGGAGAGAGTGCCCCTACTACACTGACACAGC AAACGGCCGTCCCTCCTCCCACGGATCTGCGATTCACCAATATCGGTCCGGACACTATGCGGGTCACTTG GGCCCCGCCTCCGTCCATTGAGCTAACCAACCTCTTGGTGCGCTACTCACCTGTGAAGAACGAGGAGGAT GTGGCAGAGCTGTCCATTTCACCCTCAGACAACGCCGTGGTCCTAACAAATCTCCTGCCTGGGACTGAGT ACTTAGTCAGTGTCTCCAGCGTGTACGAACAGCATGAGAGCATCCCTCTCAGAGGAAGACAGAAAACAGG TCTGGACTCCCCAACTGGTTTTGATTCTTCTGATGTCACCGCCAACTCATTCACCGTCCACTGGGTGGCT CCTCGGGCCCCCATCACCGGCTACATCATCCGCCATCACGCCGAGCATTCTGCCGGAAGACCCAGGCAAG ACCGAGTGCCGCCCTCAAGGAATTCTATCACCCTCACCAACCTTAATCCGGGCACGGAGTACATTGTCAC CATCATTGCTGTTAATGGCAGAGAGGAGAGCCCCCCACTGATTGGCCAGCAATCCACGGTTTCCGATGTC CCGAGAGATCTGGAGGTCATCGCTTCCACCCCCACCAGCCTGCTCATCAGTTGGGAACCCCCCGCCGTCT CTGTGCGCTATTACAGAATCACCTATGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAATTCACTGTGCC CGGAAGCAAGTCCACCGCCACCATCAACAACATTAAACCAGGAGCAGACTACACCATCACCCTGTATGCT GTCACTGGCCGTGGGGACAGTCCAGCCAGCAGCAAGCCAGTTTCCATCAATTATCAAACAGAAATTGACA AGCCATCCCAGATGCAGGTGACGGATGTCCAGGACAACAGCATCAGTGTCAGGTGGCTGCCTTCAACTTC TCCTGTGACAGGTTACAGAGTGACCACCGCTCCCAAAAATGGCCTAGGACCAACAAAATCTCAAACTGTC AGTCCAGATCAAACAGAAATGACCATTGAAGGTTTGCAACCCACCGTGGAGTATGTGGTTAGTGTCTATG CTCAGAACCGGAACGGAGAAAGCCAGCCCCTGGTTCAGACTGCAGTGACCAACATTGACCGCCCTAAAGG ACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCCTGGGAAAGCCCACAGGGGCAAGTTTCC AGGTACAGGGTGACCTACTCAAGCCCTGAGGATGGAATCCATGAGCTTTTCCCTGCGCCTGATGGTGACG AGGACACGGCAGAGCTGCACGGCCTCAGGCCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGG TGGCATGGAGAGCCAGCCCCTGATTGGAGTCCAGTCCACAGCCATTCCTGCGCCAACCAATCTGAAGTTC ACTCAGGTGTCACCCACCACCTTGACTGCCCAGTGGACAGCACCCAGTGTTAAGCTCACTGGCTACCGAG TGCGGGTGACCCCGAAGGAGAAGACAGGACCAATGAAAGAAATCAACCTTTCTCCAGACAGCACCTCCGT GATTGTGTCAGGGCTCATGGTGGCCACCAAGTATGAAGTCAGCGTCTATGCTCTCAAGGACACATTGACA AGCAGACCAGCTCAGGGAGTCGTCACGACTCTGGAGAATGTCAGCCCTCCAAGAAGGGCCCGTGTGACCG ACGCTACAGAAACTACCATCACTATTAGCTGGAGAACGAAGACAGAGACGATCACTGGCTTCCAAGTCGA TGCCATTCCAGCCAATGGCCAGACCCCGGTTCAGAGGACCATCAGCCCGGATGTCAGAAGCTATACTATT ACAGGTTTACAGCCAGGCACTGACTACAAGATCCACCTGTACACGCTCAACGACAATGCCCGGAGCTCTC CTGTGGTCATTGATGCCTCCACGGCCATTGATGCCCCATCCAACCTGCGGTTCCTGACCACCACACCCAA CTCCTTGCTGGTATCATGGCAGGCACCCCGTGCCAGGATTACTGGCTACATTATCAAGTATGAGAAGCCT GGATCCCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACGGAGGCCACCATCACTGGTCTGG AGCCAGGAACCGAGTACACCATCTATGTCATCGCACTGAAGAACAATCAGAAGAGTGAGCCCCTGATTGG GAGGAAAAAGACAGATGAGCTTCCCCAACTGGTTACCCTTCCACACCCCAATCTTCATGGACCAGAGATC TTGGATGTTCCCTCCACAGTTCAAAAGACCCCCTTCGTCACCAACCCTGGGTATGACACCGAAAATGGTA TTCAGCTTCCTGGCACATCCCACCAACAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGCTT TAGGCGAACCACGCCACCCACTGCGGCCACCCCCGTCAGGCTTAGGCCAAGACCATACCTGCCGAATGTA GATGAGGAGGTCCAAATCGGTCATGTTCCCAGGGGAGACGTAGACTACCACCTCTATCCTCATGTTCCGG GGCTCAATCCAAATGCCTCTACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCTTGGACGCCATTCCA GGAGAGTTCTGAGTACATCATTTCATGCCAACCTGTTGGCACTGACGAAGAGCCCTTACAGTTCCAAGTT CCTGGAACTTCTACCAGTGCGACTCTGACTGGCCTTACCAGAGGGGTCACCTACAACATCATAGTGGAGG CCCTGCACAACCAGAGGAGGCACAAGGTCCGAGAAGAGGTTGTTACTGTAGGCAACACTGTCAACGAAGG CCTGAACCAGCCTACGGATGACTCATGCTTTGACCCTTACACGGTTTCCCATTACGCCGTTGGAGAGGAA TGGGAGCGGTTATCTGACTCTGGCTTTAAGCTCACTTGCCAGTGCTTGGGCTTTGGCAGTGGTCATTTCA GATGCGATTCATCTAAATGGTGCCATGACAACGGTGTCAACTACAAGATCGGAGAGAAGTGGGATCGTCA GGGAGAAAATGGCCAGCGGATGAGCTGCACATGTCTCGGGAATGGAAAGGGAGAATTCAAATGCGATCCC CATGAAGCAACGTGTTATGACGACGGGAAGACCTACCACGTAGGAGAACAGTGGCAGAAAGAGTATCTCG GAGCCATTTGCTCCTGCACGTGTTTCGGGGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGG GGCTGCTGAACCCAGTCCCGATGGTACCACTGGCCACACCTACAACCAGTATACACAGAGATACCATCAG AGAACGAACACTAATGTAAATTGCCCAATTGAATGCTTCATGCCGTTGGACGTGCAGGCTGACAGAGATG ATTCCAGAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209525 representing NM_019143 Red=Cloning site Green=Tags(s) MLRGPGPGRLLLLAVLCLGTSVRCTETGKSKRQAQQIVQPPSPVAVSQSKPGCFDNGKHYQINQQWERTY LGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYKVGDTYERPKDSMIWDCTCIGAGRGRISCTIAN RCHEGGQSYKIGDKWRRPHETGGYMLECLCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWM MVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCVCTGNGRGEWKCERHVLQSAS AGSGSFTDVRTAIYQPQTHPQPAPYGHCVTDSGVVYSVGMQWLKSQGDKQMLCTCLGNGVSCQETAVTQT YGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHAVLVQTRGGNSNGALC HFPFLYNNRNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDL GHMMRCTCVGNGRGEWACIPYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQ CQDSETRTFYQIGDSWEKFVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQW NAPEPSHITKYILRWRPKTSTGRWKEATIPGHLNSYTIKGLTPGVIYEGQLISIQQYGHQEVTRFDFTTS ASTPVTSNTVTGETAPFSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPST ATSVNIPDLLPGRKYIVNVYQISEEGKQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGY RIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGVPRSDDVPAPK DLQFVEVTDVKVTIMWTPPNSAVTGYRVDVLPVNLPGEHGQRLPVNRNTFAEVTGLSPGVTYLFKVFAVH QGRESKPLTAQQTTKLDAPTNLQFVNETDRTVLVTWTPPRARIAGYRLTVGLTRGGQPKQYNVGPMASKY PLRNLQPGSEYTVTLMAVKGNQQSPKATGVFTTLQPLRSIPPYNTEVTETTIVITWTPAPRIGFKLGVRP SQGGEAPREVTSDSGSIVVSGLTPGVEYTYTIQVLRDGQERDAPIVNRVVTPLSPPTNLHLEANPDTGVL TVSWERSTTPDITGYRITTTPTNGQQGTALEEVVHADQSSCTFENLNPGLEYNVSVYTVKDDKESAPISD TVIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPG IDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIELTNLLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESIPLRGRQKTGLDSPTGFDSSDVTANSFTVHWVA PRAPITGYIIRHHAEHSAGRPRQDRVPPSRNSITLTNLNPGTEYIVTIIAVNGREESPPLIGQQSTVSDV PRDLEVIASTPTSLLISWEPPAVSVRYYRITYGETGGNSPVQEFTVPGSKSTATINNIKPGADYTITLYA VTGRGDSPASSKPVSINYQTEIDKPSQMQVTDVQDNSISVRWLPSTSPVTGYRVTTAPKNGLGPTKSQTV SPDQTEMTIEGLQPTVEYVVSVYAQNRNGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVS RYRVTYSSPEDGIHELFPAPDGDEDTAELHGLRPGSEYTVSVVALHGGMESQPLIGVQSTAIPAPTNLKF TQVSPTTLTAQWTAPSVKLTGYRVRVTPKEKTGPMKEINLSPDSTSVIVSGLMVATKYEVSVYALKDTLT SRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAIPANGQTPVQRTISPDVRSYTI TGLQPGTDYKIHLYTLNDNARSSPVVIDASTAIDAPSNLRFLTTTPNSLLVSWQAPRARITGYIIKYEKP GSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEI LDVPSTVQKTPFVTNPGYDTENGIQLPGTSHQQPSVGQQMIFEEHGFRRTTPPTAATPVRLRPRPYLPNV DEEVQIGHVPRGDVDYHLYPHVPGLNPNASTGQEALSQTTISWTPFQESSEYIISCQPVGTDEEPLQFQV PGTSTSATLTGLTRGVTYNIIVEALHNQRRHKVREEVVTVGNTVNEGLNQPTDDSCFDPYTVSHYAVGEE WERLSDSGFKLTCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQRMSCTCLGNGKGEFKCDP HEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAAEPSPDGTTGHTYNQYTQRYHQ RTNTNVNCPIECFMPLDVQADRDDSRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019143
ORF Size	7431 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019143.2, NP_062016.2
RefSeq Size	8331 bp
RefSeq ORF	7434 bp
Locus ID	25661
MW	272.4 kDa
Gene Summary	extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]

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