Pex1 (NM_001109220) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1559939
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209230 representing NM_001109220 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGCAGTGATCGCTTAGCCGGTGCGGGGTCTGGTGGGGCGGTCGTGACGGTGGCCTTCACGAATG CTCGGGACTGCTTCCTCCATCTGCCACGGCACCTCGTGGCCCAGCTTCACCTGCTGCAGAATCAAGCTAT AGAAGTAACCGGGGATCACCAGCCTACCTACTTGAGCTGGGTGGAAGGCAGGCATTTTAATGATCAAAGT GAAAATGTGGCAGAAATTAATAGACAAGTTGGCCAGAAGCTTGGACTCTCGAGCGGGGATCAGGTGTTTC TCAGGCCGTGTTCCCATGTGGTCTCATGCCAGCAGGTTGAGGTGGAGCCTCTCTCGGCAGACGACTGGGA GATACTGGAGCTGCACGCCATTTCCCTTGAAGAACACCTTCTAGATCAGATTCGAATAGTTTTCCCTAAA GCCATCGTTCCCATCTGGGTTGACCAGCAGACCTACATATTTATCCAAATTGTCGCTCTGATGCCAACTG CCCCTTATGGAAGGCTAGAAACTAACACCAAACTCCTTATTCAGCCAAAGACCCGCCAAGCCAAAGAGAG CACATTTCCAAAAGAAGGCGATGCACGTGGACAAGCTCATAATTATGGGCGAGAACAGAAAGGAACGGTA AAGGAATTGCAAACCAAGCAGCTTCATAAGAACACAGAGGCTGTCCCTGTATCTAATGGAAGAAACCCGG AAGTCCCAGGTGACTCAGTGAAGCCACGCTGGTGGGCTGTGCTAGGAAGCATGTTTTCCTTTGGGCCTGA CAACAAACAAGAGTCAGCTTGGGGCTCATTGGAACTCGGTGCTTTCAAAAACATGCAGTCACAGACTGTC CCTCTAGACAGTATTTTCAGAGTGTGCCAAGTTCAGCCTCCTAGTGTGCATAACACCCCTGCTAACTCCG TGTTTCACAAACACTGCACCGTCCATGTATTTCCATGGGACCAAGAATATTTTGATGTGGAGCCCAGCTT TACTGTGGTCTATGGGAAACTAGTTAAGCTACATTCTCCAAAACAACAGCAGGACAAAAGTAAGCAGGGC GTCCTGTTACCTGAAAAAGAGAAGCAACTGTCCCAGTCTCCAGGTCATAAGCGGATCGGCTCCGATGGCA GTGAGGAAGCTGCTGAGACCTGTGTGCTGAAGGTAGTCTGGAATGGACTGGAGGAGCTGAAGAATGCCAC GGAGTTCACCAAAAGTCTAGAGCCTCTCCACCATGGGAAAGTCTGGATTCCAGCTGACCTGAGAAAGAGA CTCAACATAGAAATGCATGCAGTAGTCAGGATAACTCCACTGGAAACCACCCCTGTCATCCCAAGAGCCC TGAGATTACAACCTGCAGAGAACTTACCTAGAGACGTGAGTGAAGAAGAGGTAAAAGCTGTGTTCTCATC GTGGCTACAGGAGTCTGCCACCACCATGTTTCCTTTGATAATATCAAAGCAAGAATGTATTAAGCTGGAA ATTAAAGATGGGTTGAAAGAGTTCTCTCTGAGCATAGTTCATTCTCAGGAAAAAGAGAAGGAACAAGGAA GAACTGTTTTTGTGTTGAGTCCCATTCTGCTGCAGAAGATCTCAGTACAAGTCCTTCTAGAGCCCATGAT CGGAGAACACAGTGAGGAAATTGACATTCTTCTTCCCTCTTTAACACTGAGCTCTTTGGGCGGTGTGAGT TCCTTAAGTGTATCTGCCATGGAACACATCACTCACAGTCTCCTGGGACGCCCTTTGTCTCGGCAGCTGA TGGCCCTCGTTGCCGGACTGAGGAATGGGGCTCTTTTGATCACTGGAGGAAAGGGAAGCGGGAAATCAAC GTTAGCTAAAGCCATCTGTAAGGAAGCACATGACGCTCTGGATGCCCACGTGGAGATGGTCGACTGCAAA GCTTTACGAGGAAAAAGGCTTGAAAGCATACAAAAAGCCCTAGAGGTGGCTTTCTCAGAGGCAGCCTGGA GGCAGCCATCCGTCATTCTGCTGGATGACCTGGACCTCATTGCTGGATTACCGAGCACCCCAGAGCATGA GCACAGCCCTGAAGCAGTGCAGAGCCAACGGCTTGCACACGCTTTGAACAATATGATCAAAGAGTTTGTT TCCATGGGAAGCTTGGTCGCGCTCATCGCCACGAGCCAGCTCCAGCACTCTCTCCATCCTTCCCTTGTCT CTGCTCAAGGAGTCCACACATTTCAGTGCATCCAGCACATCCAGCCTCCTGATCAGGAACAGAGATGTGA AATTCTGCATAGTGTTGTGAAGAATAAACTGGGCTGTGATATAAGCAAGTCCCCTGACTTGGACCTGAAG TGCATAGCTAAAGAGACGGAGGCGTTTGTGGCTCGAGACTTTACAGTTCTCGTGGACCGAGCCATACACT CCTCTCTCTCTCGCCAGCAGAACCCCACCAGGGAAGGATTGACTTTAACAACAGCAGACTTCCAAAAGGC TCTCCGTGGATTTCTTCCTGCTTCTCTGCGAAATGTCAACTTGCATAAACCTAGAGACCTGGGCTGGGAC AAGATCGGTGGATTACATGAAGTTCGGCAGATACTCATGGATACTATCCAGTTACCAGCCAAGTACCCAG AATTATTTGCAAACTTACCCATACGACAGAGGACGGGAATACTGCTTTATGGCCCTCCTGGGACAGGAAA AACTTTACTCGCTGGGGTGGTTGCAAGAGAGAGTGGGATGAACTTTATTAGTATTCAGGGACCAGAGTTA CTCAGCAAATACATTGGAGCAAGTGAGCAAGCTGTTCGAGATGTCTTCATCAGAGCACAGGCTGCAAAGC CCTGTATTCTTTTCTTTGATGAGTTTGAATCCATTGCTCCTCGAAGAGGTCATGACAACACAGGGGTTAC AGACCGAGTAGTTAACCAGTTGCTGACACAGTTAGATGGAGTAGAAGGCTTACAGGGAGTTTATGTGTTG GCTGCTACTAGTCGCCCTGATTTGATTGACCCCGCCTTGTTACGGCCCGGCCGACTGGATAAATGTGTAT ACTGCCCTCCTCCAGATCAGGTGTCTCGTCTTGAGATTTTAACTGTCCTCAGCAAGTCTCTACCTCTGGC GGATGACGTGGACCTTCAGCACGTGGCCTCTGTCACTGAGTCCTTCACTGGAGCGGATCTGAAAGCTCTG CTGTACAATGCACAGCTGGAGGCCTTGCAGGGACGGCTACTGCCCGGCGGGCTCCACGATGGAGGATCCA GCTCTGACAGTGACCTCAGTCTGTCTTCAATGGTCTTTCTTAACCACAGCAGTGGTTCCGACGACTCAGC TGGAGATGGAGAATGTGGCTTAGATCAATCCCTCGTTTCTCTCGAGATGTCGGAGATCCTTCCAGATGAA TCAAAATTCAATATGTACCGGCTGTACTTTGGCAGCTCCTATGAATCGGAACTTGGAAACGGAGCCTCTT GTGACTTGAGCTCACACTGTCTGTCTGCACCGAGCTCCATGACTCAGGACTTACCTGCCACTCCTGGGAA AGACCCATTGTTTACACAGCATCCTGTGTTCAGGACACCTTCCCAAGAAGGCTCCCAAGACCTTACACAG GAGCAGAGAGACCAGCTGAGAGCAGACATCAGCATCATCAAAGGCAGATACCAGAGCCAAAGTGGAGAGG ATGAATCCCTTAACCAGCCTGGACCAATCAAAACCAGTTTTGCTATTAGTCAGGCACATTTAATGACTGC GCTTGCCCACACGAGACCGTCCATTAATGAGGATGAAGGCAAGGAATTTGCTGAGCTGTATGAAAACTTT CAAAATCCGAAGAAGAGAAAAAATCAAAGTGGAACAGTGTTTCGAACTGGACAGAAAGTAACGTTAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209230 representing NM_001109220 Red=Cloning site Green=Tags(s) MWSSDRLAGAGSGGAVVTVAFTNARDCFLHLPRHLVAQLHLLQNQAIEVTGDHQPTYLSWVEGRHFNDQS ENVAEINRQVGQKLGLSSGDQVFLRPCSHVVSCQQVEVEPLSADDWEILELHAISLEEHLLDQIRIVFPK AIVPIWVDQQTYIFIQIVALMPTAPYGRLETNTKLLIQPKTRQAKESTFPKEGDARGQAHNYGREQKGTV KELQTKQLHKNTEAVPVSNGRNPEVPGDSVKPRWWAVLGSMFSFGPDNKQESAWGSLELGAFKNMQSQTV PLDSIFRVCQVQPPSVHNTPANSVFHKHCTVHVFPWDQEYFDVEPSFTVVYGKLVKLHSPKQQQDKSKQG VLLPEKEKQLSQSPGHKRIGSDGSEEAAETCVLKVVWNGLEELKNATEFTKSLEPLHHGKVWIPADLRKR LNIEMHAVVRITPLETTPVIPRALRLQPAENLPRDVSEEEVKAVFSSWLQESATTMFPLIISKQECIKLE IKDGLKEFSLSIVHSQEKEKEQGRTVFVLSPILLQKISVQVLLEPMIGEHSEEIDILLPSLTLSSLGGVS SLSVSAMEHITHSLLGRPLSRQLMALVAGLRNGALLITGGKGSGKSTLAKAICKEAHDALDAHVEMVDCK ALRGKRLESIQKALEVAFSEAAWRQPSVILLDDLDLIAGLPSTPEHEHSPEAVQSQRLAHALNNMIKEFV SMGSLVALIATSQLQHSLHPSLVSAQGVHTFQCIQHIQPPDQEQRCEILHSVVKNKLGCDISKSPDLDLK CIAKETEAFVARDFTVLVDRAIHSSLSRQQNPTREGLTLTTADFQKALRGFLPASLRNVNLHKPRDLGWD KIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVVARESGMNFISIQGPEL LSKYIGASEQAVRDVFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVL AATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILTVLSKSLPLADDVDLQHVASVTESFTGADLKAL LYNAQLEALQGRLLPGGLHDGGSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDE SKFNMYRLYFGSSYESELGNGASCDLSSHCLSAPSSMTQDLPATPGKDPLFTQHPVFRTPSQEGSQDLTQ EQRDQLRADISIIKGRYQSQSGEDESLNQPGPIKTSFAISQAHLMTALAHTRPSINEDEGKEFAELYENF QNPKKRKNQSGTVFRTGQKVTLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001109220
ORF Size	3849 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001109220.1, NP_001102690.1
RefSeq Size	4310 bp
RefSeq ORF	3852 bp
Locus ID	500006
MW	141.4 kDa

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