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Arhgef1 (NM_021694) Rat Tagged ORF Clone
CAT＃: RR209197
- TrueORF®
Arhgef1 (Myc-DDK-tagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), (10 ug)
"NM_021694" in other vectors (3)
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CNY 11,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lsc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR209197 representing NM_021694
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGAAGTCGCCGGAGGGGCGGCCCCAGGGCCTCCCCGGTCTGGCCTGGTGTCCATCATCATCGGGG CTGAGGATGAGGACTTTGAGAATGAACTGGAGGCGAACCCAGAAGATCAAAACAGCCAGTTCCAGAGCCT GGAGCAAGTGAAGCGTCGCCCAGCCCACCTCATGGCCCTCCTGCAACACGTGGCCCTGCAGTTCGAGCCA GGACCACTGCTCTGCTGCCTGCATGCTGACATGCTAAGCTCACTGGGCCCCAAAGAAGCCAAGAAGGCCT TCCTCGATTTCTATCACAGTTTCCTGGAGAAGACTGCGGTTCTGCGGGTGCCGGTCCCTCCCAGCGTTGC TTTTGAACTTGATCGTACTCGGCCTGATCTCATCTCTGAGGATGTCCAGAGGCGGTTCATACAAGAGGTG GTACAGAGCCAGCAGGCAGCCGTGACCCGTCAGCTAGAGGACTTCCGCTCCAAACGGCTCATGGGCATGA CACCCTGGGAGCAGGAACTGAGCCTGCTAGAGCCCTGGATTGGGAAAGACCGGGGCAACTATGAGGCCCG GGAGCGGCATGTTGCAGAGCGGCTGCTCTCCCACCTAGAGGAAATGCAACATACCATCTCCACAGATGAA GAAAAAAGTGCTGCTGTGGTCACTGCAATCAGCCTGTATATGCGCCACCTTGGGGTCCGGACCAAGAGTG GGGACAAGAAGTCAGGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAATCGGCGGTCAGACGAGCCCCC CAAGACAAAGAAAGGGCTGAGCAGTATCCTAGATCCTGCCCGCTGGAACCGGGGAGAGCCATCTGCTCCA GACTGTCGACATCTAAAGGTCGAGGTTGATGAGAAGCCAGGCCCTGCAGACCGGAAGGGAAGCCTGGGTA TTTCATCTCGGGACAGGACTGTTGGGACTCCTGGGCAGGACAACCCAGGAGTCTCCCTGCACCCTCTGTC TGTAGACAGCCTGGATTCCCGGGAACCAGGTGTGGATACCCCGCAGGAGCCAGGAGATACACCCCCACAA GGCCCTACAAGCCTGGAGCCCCTGGCACCCCCAGAGAGCACAGAGGACAATGGCGAGACTGAAAGCCCTG AGCCTGGAGATGATGGAGAGCCAGGGCGGTCAGGCCTGGAACAGGAACCAGAAGAACCTCCTGGGTGGAG GGAACTGGTGCCCTCAGACACCCTGCTCGGCCTGCCCAAGAACCAAGTGAAGCGGCAAGAGGTCATCAGT GAGCTGCTGGTGACGGAGGCGGCTCACGTCCGCATGCTACGGGTACTACATGACCTCTTCTACCAGCCCA TGGCAGAAGGGGGCTTCTTCCCTCTGGAGGAACTGCAGAACATCTTTCCCAGCCTGGATGAGCTCATCGA GGTGCACTCCCTGTTCCTCGATCGCTTGATGAAGCGGAGACAAGAGAGTGGCTACCTCATTGAGGAGATT GGTGATGTGCTACTGGCCCGGTTCGATGGTGCTGAGGGCTCATGGTTCCAGAAGATCTCTTCCCGCTTCT GCAGCCGCCAGTCATTCGCTCTAGAGCAGCTCAAAGCCAAGCAGCGCAAGGAGCCTCGATTCTGTGCCTT TGTGCAGGAAGCTGAGAGCCGCCCCCGCTGCCGGCGGCTGCAGTTGAAGGACATGATCCCCACTGAGATG CAGCGGCTGACCAAGTACCCACTGCTGCTGCAGAGTATTGGGCAGAACACAGAAGAGCCTGCCGAGCGGG CAAAGGTGGAACTTGCAGCTGAGTGCTGCCGTGAGATTCTGCACCACGTTAATCAAGCTGTCCGTGACAT GGAGGACCTGCTGCGGCTCAAGGATTACCAGCGGCGCCTGGACTTGACTCACCTGCGGCAGAGCAATGAC CCAATGCTGAGCGAGTTCAAGAACCTGGATATCACTAAGAAGAAGTTGGTCCATGAAGGCCCCCTCACGT GGCGACTAACCAAAGACAAAGCTGTAGAGGTCCACGTGCTATTGCTGGACGACCTGCTGCTGCTGCTCCA GCGCCAGGATGAGAGGCTGCTGCTCAAGTCACACAGCCGGACGCTGACACCCACACCCGACGGCAAGACC ATGCTGCGGCCTGTGCTCCGGCTCACCTCTGCCATGACCCGGGAGGTGGCCACTGATCACAAAGCTTTTT ACGTCATTTTTACCTGGGACCAGGAGGCCCAGATATATGAGCTGGTGGCACAGACATCGTCGGAACGCAA GAGCTGGTGTGCCCTCATCACAGAGACTGCCGGATCCCTGAAGGTCCCTGCCCCTGCCTCCCGCCCCAAA CCCCGGCCCAGCCCAAGCAGCACCCGAGAACCCCTGCTCAGCAGCTCTGAGAATGGCACTGGAGGCACAG AGGCCGCTCCAGCTGATGCCCGGACAGAGCGGATCCTCAACGACCTCCTGCCCTTCTGCAGACCAGGCCC AGAGGGCCAGCTTGCTGCCACAGCCCTCCAGAAAGTACTGTCCCTGAAACAGATCCTGTTGAGCACTGAG GAAGACAGTGGAGCAGGGCCTCCCCGCGATGGGGATGGGGTGCCTGGTGGTGGTGCCCCAGGCCCAACAC ACACCCAGGAGGTTGAGGAAAACCTGTTGAGCCTAGAAGTGGTCATCAAACAACTGGAGGAGTTAGAAGA GGAATTTTGTCGCCTAAGACCCTTCCTGTCTCAGCTTGGGGAGATTCTGTCCCCCAACCTGGCTGCACCT GAGCGCTCTGCTCAGACAGGCCTTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209197 representing NM_021694
Red=Cloning site Green=Tags(s) MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANPEDQNSQFQSLEQVKRRPAHLMALLQHVALQFEP GPLLCCLHADMLSSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEV VQSQQAAVTRQLEDFRSKRLMGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEEMQHTISTDE EKSAAVVTAISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGEPSAP DCRHLKVEVDEKPGPADRKGSLGISSRDRTVGTPGQDNPGVSLHPLSVDSLDSREPGVDTPQEPGDTPPQ GPTSLEPLAPPESTEDNGETESPEPGDDGEPGRSGLEQEPEEPPGWRELVPSDTLLGLPKNQVKRQEVIS ELLVTEAAHVRMLRVLHDLFYQPMAEGGFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEI GDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEM QRLTKYPLLLQSIGQNTEEPAERAKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSND PMLSEFKNLDITKKKLVHEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKT MLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTSSERKSWCALITETAGSLKVPAPASRPK PRPSPSSTREPLLSSSENGTGGTEAAPADARTERILNDLLPFCRPGPEGQLAATALQKVLSLKQILLSTE EDSGAGPPRDGDGVPGGGAPGPTHTQEVEENLLSLEVVIKQLEELEEEFCRLRPFLSQLGEILSPNLAAP ERSAQTGLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021694 |
| ORF Size | 2757 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021694.3, NP_067726.3 |
| RefSeq Size | 3243 bp |
| RefSeq ORF | 2760 bp |
| Locus ID | 60323 |
| MW | 102.8 kDa |
| Gene Summary | mouse homolog is an oncogene with similarity to the Dbl family of guanine nucleotide exchange factors [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN209197 | Arhgef1 (untagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), (10 ug) |
CNY 11,880.00 |
|
| RR209197L3 | Lenti ORF clone of Arhgef1 (Myc-DDK-tagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), (10 ug) |
CNY 13,490.00 |
|
| RR209197L4 | Lenti ORF clone of Arhgef1 (mGFP-tagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 1 (Arhgef1), (10 ug) |
CNY 14,920.00 |
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