Map1lc3b (NM_022867) Rat Tagged ORF Clone
CAT#: RR209172
- TrueORF®
Map1lc3b (Myc-DDK-tagged ORF) - Rat microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10 ug)
"NM_022867" in other vectors (3)
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CNY 1,800.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LC3B; Map1lc3; Mpl3; zbs559 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209172 representing NM_022867
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTCCGAGAAGACCTTCAAACAGCGCCGGAGCTTCGAACAAAGAGTGGAAGATGTCCGGCTCATCC GGGAGCAGCACCCCACCAAGATCCCAGTGATTATAGAGCGATACAAGGGTGAGAAGCAGCTGCCCGTCCT GGACAAGACCAAGTTCCTTGTACCTGATCACGTGAATATGAGCGAACTCATCAAGATAATTAGAAGGCGC CTGCAGCTCAATGCTAACCAAGCCTTCTTCCTCCTGGTGAATGGGCACAGCATGGTGAGTGTGTCCACAC CCATCTCTGAAGTGTACGAGAGCGAGAGAGATGAAGACGGCTTCCTGTACATGGTCTATGCCTCCCAGGA GACGTTCGGGACAGCACTGGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209172 representing NM_022867
Red=Cloning site Green=Tags(s) MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRR LQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTALAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022867 |
ORF Size | 375 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_022867.2, NP_074058.2 |
RefSeq Size | 1768 bp |
RefSeq ORF | 378 bp |
Locus ID | 64862 |
UniProt ID | Q62625 |
MW | 14.6 kDa |
Gene Summary | may mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system [RGD, Feb 2006] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Ginseng extract reduces tacrolimus-induced oxidative stress by modulating autophagy in pancreatic beta cells.
,null,
Laboratory investigation; a journal of technical methods and pathology
,PubMed ID 28759009
[Map1lc3b]
|
Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus
,Lim, SW;Jin, L;Jin, J;Yang, CW;,
Sci Rep
,PubMed ID 27436514
[Map1lc3b]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209172 | Map1lc3b (untagged ORF) - Rat microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10 ug) |
CNY 3,990.00 |
|
RR209172L3 | Lenti ORF clone of Map1lc3b (Myc-DDK-tagged ORF) - Rat microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10 ug) |
CNY 6,080.00 |
|
RR209172L4 | Lenti ORF clone of Map1lc3b (mGFP-tagged ORF) - Rat microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10 ug) |
CNY 6,650.00 |
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