Col17a1 (NM_001106366) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209139 representing NM_001106366 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGACCAAGAAAAGCATGCGAGATGGCACTGAAGTCACTGAGAGAATTGTCACGGAAACAGTAA CCACAAGACTCACATCCTTACCACCAAAAGGGAGCACCAGCAATGGATACACTAAGACAGGCTCTCTGGG TGGAGGAAGCCGGCTAGAGAAACAAAGTTTGACCCATGGAAGCAGCGGCTACATCAACTCAAGCGGAAGC CTCCGAGGCAACGCTTCCACCTCCAGTTACAGGAGAGCTCACTCACCTGCCTCGACCCTGCCCAACTCTC CCGGCTCCACCTTTGAAAGGAAAACTCACATGAGCCGCCATGGAACTTACGAAGGGAGCTCCAGTGGCAA CTCCTCACCTGAGTATCCACGGAAGGAACTCGCCTCTTCTTCAACCAGAGGACGAAGCCAGACACGAGAG AGCGAAATTCGAGTTCGACTACAGAGTGCGTCGCCATCCACCAGATGGACAGAACTGGATGAAGTGAAGC GCTTGCTGAAGGGGAGCCGATCTGCCAGTGCCAGCCCCACCAGGAACACCTCCAACACACTCCCTATCCC CAAGAAAGGCACCGTAGAAACCAAAACGGTGACAGCGAGCTCCCACTCAGTGTCAGGAACCTATGATTCA ACAATACTGGACAGCAACTTTCCCCCCCACATGTGGTCTTCCACCTTGCCGGCAGGGTCCTCCATGGGGA CCTATCACAACAACATAGCGACCCAGAGCACATCCCTCGTCAACACCAATGCCTACTCAGCAGGATCAGT CTTTGGAGTGCCAAATAACATGGCGTCCTGCTCTCCCACCCTGCACCCAGGACTCAGCAGCTGCTCCTCA GTGTTTGGCATGCAGAACAATCTGGCCCCCTGCTCTTCTGTCCTCTCCCATGGCACAACCACGGCTTCCA CAGCATATGGTGTGAAGAAAAACGTGCCCCAGCCACCCACTGTCACCAGCACCGGCGTGTCCACCTCTGC CGCCTGCACCACCAGTGTCCAGAGCGACGACCTCTTGCATAAAGACTGCAAGTTCCTGATTCTGGAGAAA GACAACGTGCCCGCCAAGAAGGAGATGGAGCTCTTGATCATGTCCAAGGACAGTGGGAAGGTCTTCACTG CCTCCCCCGCCAGCATCTCTTCAACTTCTTTTTCAGAGGACACCCTGAAAAAGGAGAAGCAGGCTGCGTA TGCCGCTGACACATGTCTAAAGGCAGATGTGAACGGAGACCTAAACACTGTGTCTACGAAGAGCAAAGCC ATCTCGGCAGAAAACCACAGCTACGACCGAGGTGTCGGTGGTGGCAGAGGCAAAAGCGGAGTCGCCGGCG GTGGTGGTGGTGGTGGCGGCGGCGGCAGCGGCGGCGGAACATGGGGGGCTGCACCTGCCTGGTGCCCCTG CGGCTCCTGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACTTGGCTGCTGCTGCTGGGTCTG CTCTTCGGCCTCATTGCCCTGGCGGAGGAGGTAAGGAAGCTGAAGGCCCGCGTGGAGGAGCTGGAAAAGA CCAAGGTGCTATATCATGACATCGTCAAGACGGACCGAAGCAGCAGGGACCGCCTCCAGGGTGAGGCACC CAGCCTGGGACCTGGATTAAGCAAGGCTGAAATGGACGACTACGGCCAGGAGGCCATCTGGCTGTTTGTA AGGAACAAGCTGATGACAGAGCAGGAGAACGGAAATCTCAGAGGAAGTCCTGGTCCAAAAGGTGACACGG GGAGTCAAGGACCTAAAGACCGTGGCCTTCCTGGGATCCCAGGTACCCCTGGGCCCCTGGGCCACCCCGG CCCGGAAGGACCAAAGGGACAAAAAGGCAGCATTGGAGATCCAGGCATGGAAGGACCCATAGGCCAGAGA GGACTAGCAGGCCCCATGGGACCTCCTGGTGAACCTGGGCCTCCGGGGTCTGGAGAGAAAGGAGACAGAG GGATTGCTGGAGAACAAGGTCCTCGGGGTCTTCCCGGTGTCCCGGGTTCTCCGGGTCTCAGAGGTACCAG TGGCTCTCCTGGTCCCCAGGGCCCCCCAGGCTCTGTGGGTCCCCAAGGGCTCCGAGGTGAAGTGGGACTT CCGGGTGTCAAAGGTGACAAAGGACTCGTGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAAGGAC CCAGAGGCCTCACAGGGGAGCCTGGCATCCGAGGTCTGCCTGGAGCTGTGGGTGAACCTGGAGCCAAAGG AGCAATGGGTCCCGCTGGCCCCGATGGACAGCAAGGTTCCAGAGGTGAACAAGGCCTGACTGGGATGCCT GGAGCCCGGGGCCCACCAGGACCCACCGGAGACCCAGGGAAGCCAGGTCTCACAGGACCCCAAGGACCTC AGGGACTTCCTGGTACCCCTGGCCGACCAGGGACTAAAGGTGAACCTGGCGCTCCAGGCAGAGTCATGAC TTCAGAGGGATCCTCAGCAATCACTGTGCCGGGACCTCCCGGACCTCCCGGTGCCATGGGACCCCCAGGA CCTCCAGGGACTCCAGGTCCGGCTGGCCCTGCTGGTCTCCCAGGACAACAAGGCCCACGAGGGGAGCCGG GACTTGCTGGGGACTCCTTCCTAAACAGTGGCAGTTCCATCTCCGAAGTCCTCTCTGCCCAAGGTGTTGA CTTTCGAGGTCCCCCTGGCCCACCTGGCCCACGAGGGCCACCAGGGCCTTCCATTCCAGGCCCACCAGGA CCCAGAGGCCCACCAGGGGAAGGCGTACCAGGCCCACCCGGCCCACCAGGATCCTTCCTGACTGACTCGG AAACCTTCTTCTCTGGCCCTCCAGGTCCACCTGGCCCCCCAGGTCCCAAGGGAGACCAAGGTGACCCCGG TGTCCCAGGCACTCCTGGCATCCCTGGTGGTCTTTCTCATGGGACGTCGTCAGGCACTATGTACGCACAG GGCCCACCTGGTCCCCCAGGACCTCCTGGGCCTCCAGGATCTCTCAGCAGCTCCGGCCAGGAACTCCAGC GCTATATTGCAGAGTACATGCAAAGTGACAACATCAGGACTTATCTCTCAGGAGTTCAGGGTCCCCCCGG CCCACCAGGTCCCCCAGGGCCTGTCATCACCATCACAGGGGAGACTTTCGACTACTCCCAGCTGGCAAGC CAGGTTGTGAGTTACTTGCGATCATCAGGCTATGGTGTTGGCTTGTCCTCTGCCTCCTCCTCAGAAGATA TCTTGGCCATGCTGAGACGCAATGATGTGTGGCAGTACCTACGTCAGCACCTGGTGGGCCCTCCGGGTCC CCCGGGACCACCAGGAGTCAGTGGAGATGGGTCCCTCCTGTCTTTGGACTATGGAGAGCTAAGCAGACAC ATTCTCAACTATATGTCAAGTTCTGGGATCAGCTTTGGGCATCCTGGACCACCGGGCCCCCCTGGCCTGC CAGGAACATCCTATGAGGAGCTACTGACCATGCTCCGAGCTGCTGGTCTGTCCTCCATCGCAGGCCCCCC GGGCCCCCCAGGTCCCCCAGGTCCTCGAGGGCCCCCGGGTGTGTCAGCAGCTCTGGCCACCTACGCAGCT GAGAACAGTGACAACTTCCGCAGTGAGCTGATTAGCTACCTTACAAGTCCTGATGTCCGCAGCTTCATCG TTGGCCCTCCAGGTCCTCCAGGGCCACAGGGACCACCTGGAGACTACAACTGGGGTAGCGGCTCCTCAGC CAGAAGGGGCACTTCCTACAGCTCTTCCATGGGCACAGGGAGAACCAATGGTGGCTCCCTGGGCGAAGGT GGAGCCTTTGGTGCAGGAGATGGGGGCCCCTACGGCACTGACGTCGGCCCAGGCGGAGGCTACGGGGCAG CTGCAGGTGGTGGTGTATATGGCACCAATGGCGAATCATTCCGGGGCAGCTTCACTGGAGACCTAGATTA TAACACGCTGGCATTGCGGGTGTCGGAGAGCATGCAGCGTCAGGGTCTGCTACAAGGGATGGCCTACACT GTCCAGGGCCCACCAGGGCCACAAGGCCCTCCTGGCATCAGCAAGGTCTTCTCTGCCTACAGCAACGTGA CACAAGACCTCATGGACTTCTTCCGAACATATGGCGCTATCCCAGGACCCCCTGGGCAGAAAGGAGACAT GGGAACCCCAGGTCCCAAAGGTGACAGGGGCCCTGCTGGACCACGAGGTCCTCCGGGGCCCCCGGGCCCC CGAGGGAACAAAGGAGAGAAAGGGGATAAAGGTGACCAAGTCTATACTGGGAGGAGGAAGAGAAGTATTG CCATCAAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209139 representing NM_001106366 Red=Cloning site Green=Tags(s) MDVTKKSMRDGTEVTERIVTETVTTRLTSLPPKGSTSNGYTKTGSLGGGSRLEKQSLTHGSSGYINSSGS LRGNASTSSYRRAHSPASTLPNSPGSTFERKTHMSRHGTYEGSSSGNSSPEYPRKELASSSTRGRSQTRE SEIRVRLQSASPSTRWTELDEVKRLLKGSRSASASPTRNTSNTLPIPKKGTVETKTVTASSHSVSGTYDS TILDSNFPPHMWSSTLPAGSSMGTYHNNIATQSTSLVNTNAYSAGSVFGVPNNMASCSPTLHPGLSSCSS VFGMQNNLAPCSSVLSHGTTTASTAYGVKKNVPQPPTVTSTGVSTSAACTTSVQSDDLLHKDCKFLILEK DNVPAKKEMELLIMSKDSGKVFTASPASISSTSFSEDTLKKEKQAAYAADTCLKADVNGDLNTVSTKSKA ISAENHSYDRGVGGGRGKSGVAGGGGGGGGGGSGGGTWGAAPAWCPCGSCCSWWKWLLGLLLTWLLLLGL LFGLIALAEEVRKLKARVEELEKTKVLYHDIVKTDRSSRDRLQGEAPSLGPGLSKAEMDDYGQEAIWLFV RNKLMTEQENGNLRGSPGPKGDTGSQGPKDRGLPGIPGTPGPLGHPGPEGPKGQKGSIGDPGMEGPIGQR GLAGPMGPPGEPGPPGSGEKGDRGIAGEQGPRGLPGVPGSPGLRGTSGSPGPQGPPGSVGPQGLRGEVGL PGVKGDKGLVGPPGPKGDQGEKGPRGLTGEPGIRGLPGAVGEPGAKGAMGPAGPDGQQGSRGEQGLTGMP GARGPPGPTGDPGKPGLTGPQGPQGLPGTPGRPGTKGEPGAPGRVMTSEGSSAITVPGPPGPPGAMGPPG PPGTPGPAGPAGLPGQQGPRGEPGLAGDSFLNSGSSISEVLSAQGVDFRGPPGPPGPRGPPGPSIPGPPG PRGPPGEGVPGPPGPPGSFLTDSETFFSGPPGPPGPPGPKGDQGDPGVPGTPGIPGGLSHGTSSGTMYAQ GPPGPPGPPGPPGSLSSSGQELQRYIAEYMQSDNIRTYLSGVQGPPGPPGPPGPVITITGETFDYSQLAS QVVSYLRSSGYGVGLSSASSSEDILAMLRRNDVWQYLRQHLVGPPGPPGPPGVSGDGSLLSLDYGELSRH ILNYMSSSGISFGHPGPPGPPGLPGTSYEELLTMLRAAGLSSIAGPPGPPGPPGPRGPPGVSAALATYAA ENSDNFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDYNWGSGSSARRGTSYSSSMGTGRTNGGSLGEG GAFGAGDGGPYGTDVGPGGGYGAAAGGGVYGTNGESFRGSFTGDLDYNTLALRVSESMQRQGLLQGMAYT VQGPPGPQGPPGISKVFSAYSNVTQDLMDFFRTYGAIPGPPGQKGDMGTPGPKGDRGPAGPRGPPGPPGP RGNKGEKGDKGDQVYTGRRKRSIAIKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106366
ORF Size	4281 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106366.1, NP_001099836.1
RefSeq Size	5433 bp
RefSeq ORF	4284 bp
Locus ID	294027
MW	143.6 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.