Myh2 (NM_001135157) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Myh1; Myh2a; MyHC-2A; MyHC-IIA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209111 representing NM_001135157 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACTCTGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC GAATCGAGGCCCAGAATAGGCCTTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGAGTCCTT TGTCAAAGGAACCATTCAGAGCAAAGACGCAGGGAAAGTGACTGTGAAAACTGAAGCAGGAGCGACCCTG ACGGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTCCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACAC CTACTCGGGCCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCAGTGTACAACCCTGAGGTGGTG GCTGCCTACCGTGGCAAAAAGCGACAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAATGCCTACC AGTTCATGCTAACTGACCGGGAGAATCAGTCAATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGAGAAAAAGAAGGAGGAGGTG ACTTCTGGCAAAATGCAGGGGACCCTGGAAGATCAAATCATCAGTGCCAACCCTCTGCTGGAGGCCTTTG GGAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATTCACTTTGGCAC CACGGGGAAACTGGCATCCGCTGACATCGAGACATATCTGCTAGAGAAGTCCCGGGTCACTTTCCAGCTT AAGGCGGAAAGGAGCTACCATATATTTTATCAAATCACATCCAACAAGAAGCCAGAGCTGATCGAAATGC TGCTGATCACCACAAACCCATACGATTACCCATTCGTCAGTCAAGGGGAGATCAGTGTGGCCAGCATTGA TGACCAGGAAGAACTGATGGCCACGGATAGTGCTATTGACATTCTGGGCTTTACAAATGATGAAAAGGTC TCCATCTATAAGCTCACGGGAGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAAG AACAGGCTGAGCCGGATGGCACGGAAGTGGCTGACAAGGCTGCCTATCTTCAGGGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTTTGTTACCCCAGGGTCAAGGTCGGCAATGAGTACGTCACCAAAGGCCAGACTGTG GAGCAGGTGACCAACGCTGTGGGTGCCCTGGCCAAGGCCATGTACGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAACAGCATCTT GGAAAGTCCGCCAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAAGCCGAGGCCCACTTCTCCCTCATCC ACTATGCGGGCACCGTGGATTACAACATCACTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC CGTGGTGGGGCTGTACCAGAAGTCTTCAATGAAAACTCTTGCTTACCTCTTCTCTGGAGCTCAAACTGCT GAAGCAGAGGCAAGTAGTGGTGGAGCTGCCAAGAAAGGTGCTAAGAAGAAAGGGTCCTCCTTCCAGACAG TGTCTGCCCTGTTCAGAGAGAACTTGAACAAGCTGATGACCAACCTCAGGAGCACCCACCCTCATTTTGT GAGGTGTATCATCCCCAATGAAACCAAGACACCTGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG AGGTGTAACGGGGTGCTAGAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCAGGATCCTCTACGCAG ACTTCAAGCAGAGATACAAGGTGTTAAATGCAAGTGCCATCCCTGAGGGTCAGTACATAGATAGCAAGAA GGCTTCTGAGAAGCTCCTTGGCTCCATCGACATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTGATTACCC GGACGCAGGCCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAGATGGTGGAGCGAAGAGAGTC CATCTTCTGCATCCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTC TTCAAGATCAAACCACTGCTGAAGAGCGCTGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCC AGAAAACCAAGGATGAACTTGCCAAATCGGAGGCAAAGAGGAAGGAACTTGAAGAAAAGATGGTGTCCCT GTTGAAAGAAAAAAATGACTTGCAACTCCAAGTTCAGGCTGAAGCTGAAGGCTTGGCTGATGCTGAGGAA AGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACTGAGAGAGCTG AGGATGAGGAAGAGATCAACGCCGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT GAAGAAAGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC AAGGTGAAAAACCTCACAGAGGAGATGGCGGGCCTGGACGAAACCATCGCCAAGCTGACCAAGGAGAAGA AGGCCCTGCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGATCTTGAAGGGTCCTTGGAGCAAGAAAAGAAG CTTCGCATGGACCTAGAAAGAGCCAAGAGAAAACTTGAGGGTGACTTGAAGTTAGCCCAGGAGTCCATCA TGGACATTGAAAATGAGAAACAGCAGCTTGATGAAAGGCTCAAAAAGAAAGAGTTTGAAATGAGCAACCT GCAGAGCAAGATCGAGGATGAGCAGGCCATCGGCATTCAACTGCAGAAGAAGATCAAGGAGTTGCAGGCT CGCATTGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGCGGCTGGAAGAAGCCGGCGGGGCCACTTCAGCCCA GATCGAGATGAACAAGAAGAGAGAGGCCGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTG CAGCACGAAGCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGA TTGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGACGA CCTCGCTAGTAATGTAGAGACAGTGTCTAAGGCCAAGGGAAACCTAGAGAAGATGTGCCGCACCCTGGAG GACCAGGTGAGTGAGCTGAAGTCGAAGGAGGAGGAACAACAGCGCCTGATCAACGACCTGACCACCCAGA GAGGACGCCTGCAGACCGAATCCGGTGAATTTTCCCGGCAGCTTGATGAGAAGGAAGCGCTGGTTTCTCA GTTATCAAGGGGCAAGCAAGCATTTACCCAACAGATTGAGGAGCTGAAGAGGCAGCTGGAAGAGGAAGTA AAGGCCAAGAACGCGCTAGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTTCGGGAACAGT ACGAGGAGGAGCAGGAATCTAAGGCCGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTGGCCCA GTGGAGGACCAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAGAAAAAGCTG GCTCAGCGTCTGCAGGCGGCTGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGTGCTTCCCTGGAGAAGA CTAAGCAGCGGTTGCAGAACGAGGTGGAGGACCTCATGCTGGACGTAGAGAGGACCAACGCTGCCTGTGC TGCCCTGGACAAGAAGCAGAGAAACTTCGACAAGATCCTGGCAGAGTGGAAGCAGAAGTATGAGGAAACA CATGCTGAGCTGGAGGCATCCCAGAAGGAAGCCCGCTCCCTGGGTACTGAGCTCTTCAAGATGAAGAACG CCTACGAGGAGTCCCTGGATCAGCTGGAAACCCTGAAGCGAGAGAATAAGAACTTACAGCAGGAGATTTC TGACCTCACGGAACAGATTGCAGAAGGAGGAAAGCGCATCCACGAACTGGAGAAAATAAAGAAACAAGTC GAACAAGAGAAGTGTGAACTTCAGGCTGCTCTAGAAGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA AGATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA TGAGGAGATCGACCAGCTGAAGAGAAACCACATTCGAGTCGTGGAGTCCATGCAAAGCACGCTGGACGCC GAGATCAGGAGCAGGAACGACGCCATCAGGATCAAGAAGAAGATGGAGGGAGATCTCAATGAGATGGAAA TCCAGCTGAACCACGCCAACCGCATGGCTGCTGAGGCCCTGAGAAACTACAGGAACACCCAAGGCATCCT CAAGGACACCCAGCTGCACCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG GTGGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAGCAGACTGAGA GGAGCAGGAAGATTGCAGAGCAGGAACTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAGATCCAGGGAGAGATGGAGGAC ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAAGCCATCACTGACGCCGCCATGATGGCGG AGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACATGGAGCAGACGGT GAAGGACCTGCAACTGCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG AAACTGGAGGCCAGAGTGCGTGAACTGGAGGGTGAGGTAGAGAGTGAGCAGAAGCGGAATGTTGAGGCTG TCAAAGGGCTTCGAAAACACGAGAGGCGAGTGAAGGAGCTTACTTACCAGACAGAAGAAGACCGAAAAAA TATCCTCAGGCTTCAAGATTTGGTGGATAAACTCCAAGCAAAAGTAAAATCTTACAAGAGACAAGCTGAG GAGGCTGAGGAACAATCCAACACAAATCTATCCAAGTTCCGCAAGCTGCAGCATGAGCTGGAGGAAGCCG AGGAGCGGGCTGACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGCGAGGTTCACACTAA AGTCATAAGTGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209111 representing NM_001135157 Red=Cloning site Green=Tags(s) MSSDSEMAVFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSKDAGKVTVKTEAGATL TVKEDQIFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEV TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNDEKV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEYVTKGQTV EQVTNAVGALAKAMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAQTA EAEASSGGAAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQL RCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQYIDSKKASEKLLGSIDIDHTQYKFGHTKV FFKAGLLGLLEEMRDDKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLF FKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVSLLKEKNDLQLQVQAEAEGLADAEE RCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATEN KVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKK LRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDERLKKKEFEMSNLQSKIEDEQAIGIQLQKKIKELQA RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATL QHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLE DQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEV KAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKL AQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEET HAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQV EQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDA EIRSRNDAIRIKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAM VERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMED IVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQ KLEARVRELEGEVESEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAE EAEEQSNTNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135157
ORF Size	5826 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135157.1, NP_001128629.1
RefSeq Size	6041 bp
RefSeq ORF	5829 bp
Locus ID	691644
MW	223.3 kDa
Gene Summary	mouse homolog is a heavy chain skeletal muscle myosin [RGD, Feb 2006]

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