Sptbn2 (NM_019167) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Spnb3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209033 representing NM_019167 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCACCCTGTCACCCACTGACTTCGACAGCTTGGAGATCCAGGGCCAGTACAGTGACATCAACA ACCGCTGGGACCTGCCCGACTCAGATTGGGACAATGACAGCAGTTCAGCCCGCCTCTTTGAGAGGTCCAG AATTAAGGCCCTGGCAGATGAGCGAGAAGCCGTGCAGAAGAAAACCTTCACCAAGTGGGTGAACTCCCAC CTGGCCCGGGTGACATGCCGGGTGGGAGACCTGTACAGCGACCTGCGGGACGGGCGCAACCTCCTGAGGC TCCTGGAGGTGCTCTCGGGAGAGACCCTGCCAAAACCCACCAAGGGCCGGATGCGGATTCACTGCCTGGA GAATGTCGACAAAGCACTGCAGTTCCTGAAGGAGCAGAAGGTGCACCTGGAAAACATGGGCTCCCACGAC ATTGTGGATGGGAACCACCGTCTGACCCTTGGGCTAGTGTGGACCATCATCCTCCGATTTCAGATCCAAG ACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGATGCCCTGCTGCTGTGGTGCCA GATGAAGACTGCAGGGTATCCCAATGTCAATGTGCACAACTTTACCACCAGTTGGAGAGATGGGCTGGCC TTTAATGCCATTGTGCACAAACACCGGCCAGACCTGTTGGATTTTGAGTCCCTGAAGAAGTGTAACGCAC ACTACAATCTGCAGAATGCTTTCAATCTGGCTGAAAAGGAACTTGGCCTGACGAAGCTCCTGGATCCTGA AGATGTGAACGTAGACCAACCCGATGAGAAGTCCATCATCACCTACGTGGCCACTTACTACCACTACTTC TCGAAGATGAAGGCCCTGGCTGTGGAAGGCAAAAGGATTGGCAAGGTCCTGGACCATGCCATGGAGGCAG AACACCTGGTAGAGAAATATGAGTCCCTGGCCTCTGAACTGCTGCAGTGGATCGAGCAAACGATTGTGAC CCTCAATGACCGACAGCTGGCCAACTCCCTGAGTGGCGTCCAGAACCAGCTGCAGTCTTTCAATTCCTAC CGCACGGTGGAGAAGCCACCCAAGTTCACAGAGAAAGGGAACTTGGAGGTGTTGCTCTTCACCATCCAGA GTAAGCTGCGGGCCAACAACCAGAAAGTCTACACACCACGCGAAGGCCGGCTCATCTCGGACATCAACAA GGCCTGGGAGCGGCTAGAGAAAGCCGAACATGAGCGAGAGCTGGCCCTGCGCACGGAGCTGATCCGCCAG GAGAAGCTGGAGCAACTGGCTGCTCGCTTCGACCGCAAGGCTGCCATGCGGGAGACCTGGCTCAGTGAGA ACCAGCGCCTCGTCTCCCAGGACAACTTTGGCCTGGAGCTGGCAGCAGTGGAGGCAGCAGTGCGGAAGCA TGAAGCCATTGAGACAGACATTGTGGCCTACAGCGGCCGGGTGCAAGCGGTGGACGCCGTAGCCGCAGAA CTGGCCGCTGAGCACTACCATGACATTAAGCGCATTGCGGCGCGGCAGAACAACGTGGCCCGGCTCTGGG ACTTCTTACGAGAGATGGTGGCCGCCCGCCGTGAGCGGCTCCTTCTCAACCTGGAGCTGCAGAAGGTGTT TCAGGACCTGCTCTACTTCATGGACTGGATGGCAGAGATGAAGGGCCGGCTGCAGTCTCAGGACCTAGGC AAGCATCTGGCTGGAGTGGAAGATCTACTGCAACTACACGAACTGGTGGAGGCGGACATTGCAGTTCAGG CTGAGAGGGTGCGAGCGGTCAGCGCCTCTGCCCTGCGCTTCTGCGACCCCGGGAAAGAGTATAGACCATG TGACCCACAGCTAGTGTCAGAGAGGGTAGCCACTCTGGAGCAGAGCTATGAGGCCCTGTGCGAATTGGCA GCAACTCGAAGGGCCCGACTGGAAGAGTCCCGTCGTCTCTGGAGGTTCCTCTGGGAAGTGGGTGAGGCCG AGGCCTGGGTTCGGGAGCAGCAGCACCTCCTGGCCTCAGCTGAGACAGGCCGGGACCTGACTGGTGTCCT CCGCCTGCTCAATAAGCACACAGCCCTACGGGGTGAGATGAGTGGCCGCCTGGGGCCCCTGAAGCTCACC CTGGAACAAGGTCAGCAGTTAGTTGCCGAGGGCCACCCTGGAGCTAACCAAGCCTCAACCCGTGCAGCAG AGCTCCAGGCCCAGTGGGAGCGACTAGAAGCCCTGGCCGAGGAGCGAGCCCAGCGGCTAGCACAGGCTGC CAGCCTCTACCAGTTCCAGGCAGATGCCAATGACATGGAGGCTTGGTTGGTGGACGCACTACGCCTGGTA TCTAGCCCTGAGGTAGGGCACGATGAGTTCTCCACGCAGGCCCTGGCCAGGCAGCACAGGGCCCTTGAGG AGGAGATCCGAGCCCACCGGCCTACACTGGATGCCTTGAGGGAGCAGGCTGCAGCCCTGCCACCTGCACT GAGCCACACACCTGAGGTACAGGGCAGGGTGCCCACTCTGGAGCAGCACTATGAGGAGCTGCAGGCCCGG GCAGGTGAGCGTGCACGAGCCCTGGAAGCAGCCCTGGCGTTCTATACCATGCTCAGCGAGGCCGGGGCTT GTGGGCTCTGGGTAGAGGAGAAGGAGCAGTGGCTCAACGGGCTGGCCCTACCTGAGCGCCTGGAGGACCT GGAGGTGGTCCAACAGAGGTTTGAGACCTTAGAGCCCGAAATGAACGCCCTGGCTGCACGGGTTACTGCT GTCAGTGACATAGCTGAGCAGTTGCTGAAGGCCAGTCCACCAGGCAAGGACCGCATCATTGGCACCCAGG AGCAGCTCAACCAAAGGTGGCAGCAGTTCAGGTCCCTGGCAGATGGCAAAAAGGCAGCTCTGACATCAGC CCTGAGCATCCAGAATTACCACCTAGAGTGCACAGAGACCCAGGCCTGGATGAGAGAAAAGACCAAGGTC ATTGAGTCTACCCAGGACCTAGGCAATGATCTAGCTGGTGTGCTGGCCCTGCAGCGGAAGCTGGCAGGCA CTGAGAGAGATCTGGAAGCCATCTCTGCCCGGGTGGGTGAGCTGACCCAAGAGGCAAATGCTTTGGCTGC TGGGCACCCAGCCCAAGCCCCTGCCATCAACACACGGCTTGGAGAGGTTCAAACTGGATGGGAGGATCTT CGGGCAACCATGAGGCGGAGAGAAGAGTCCCTGGGTGAGGCTCGACGGCTGCAAGATTTCCTGCGCAGCT TGGATGACTTCCAGGCCTGGCTAGGCCGCACACAGACTGCTGTAGCCTCTGAGGAAGGACCAGCCACCCT TCCAGAGGCAGAAGCCCTCTTAGCCCAGCATGCAGCTCTGCGGGGAGAGGTGGAGAGAGCCCAGAGCGAG TACAGCCGCCTCAGGACCTTGGGCGAGGAGGTGACCAGAGACCAGGCTGATCCCCAATGCCTCTTCCTCA GACAGAGGCTGGAAGCCCTTGGAACCGGCTGGGAGGGAGCTGGTCGCATGTGGGAGAGCCGGCAAGGCCG CTTGGCCCAGGCCCATGGCTTCCAGGGGTTTTTGAGAGATGCTCGCCAGGCTGAGGGAGTTCTCAGCAGC CAGGAATATGTTCTATCTCACACGGAGATGCCAGGGACACTGCAGGCGGCGGATGCAGCCATTAAAAAGC TGGAAGACTTCATGAGCACCATGGACGCCAATGGAGAGCGCATCCGTGGACTCCTGGAGGCTGGCCGTCA GCTGGTGTCCAAGGGCAATATCCATGCTGAGAAGATCCAAGAGAAGGCAGACTCCATCGAGAAGAGGCAC AGAAAGAACCAGGAGGCCGTGCAGCAGCTTCTAGGCCGCCTTCGGGACAACCGAGAGCAGCAGCACTTCT TGCAAGACTGTCAGGAGCTGAAACTCTGGATTGACGAGAAGATGCTGACAGCTCAGGATGTGTCCTATGA TGAGGCACGCAACCTGCACACCAAGTGGCAAAAACACCAGGCATTCATGGCCGAGCTGGCAGCCAACAAG GACTGGCTGGACAAAGTGGACAAGGAAGGGCGGGAGCTGACTCTTGAAAAGCCAGAACTCAAAGTCCTAG TGTCAGAGAAGCTGGAGGACCTGCACAGGCGCTGGGATGAACTGGAGACTACCACCCAAGCCAAGGCCCG CAGTCTTTTTGATGCTAACCGGGCAGAGCTATTTGCCCAAAGCTGTTCTGCCCTGGAGAGCTGGCTGGAG AGCCTGCAGGCCCAGCTGCACTCAGATGACTATGGCAAGGACCTCACCAGTGTCAACATTCTGCTAAAGA AGCAACAGATGCTGGAACGAGAGATGGCTGTGAGAGAGAAGGAGGTAGAGGCTATCCAGGCCCAGGCAAA AGCCCTGGCCCAGGAAGACCAAAGTGCAGGAGAGGTGGAAAGGACCTCCAGAGCTGTGGAGGAGAAGTTC AGGGCCTTGTGTCAGCCCATGAAGGACCGCTGCCGGCGCCTGCAAGCCTCCCGAGAGCAGCACCAGTTCC ACCGGGATGTGGAGGATGAGATACTGTGGGTGACCGAGCGGCTTCCCATGGCCAGCTCTCTGGAGCATGG CAAGGACTTGCCCAGCGTCCAGCTTCTCATGAAGAAAAACCAGACTCTGCAGAAGGAGATCCAGGGCCAT GAGCCCCGGATTGCAGACCTCAAAGAGAGACAGCGCACTCTGGGAACAGCAGCAGCGGGTCCAGAGCTGG CTGAGCTCCAGGAAATGTGGAAACGCCTGAGCCATGAGCTGGAGCTTCGGGGTAAACGACTGGAGGAGGC CCTTCGAGCCCAGCAATTCTATCGTGACGCTGCAGAGGCCGAGGCTTGGATGGGGGAGCAGGAGTTACAT ATGATGGGCCAGGAAAAGGCCAAGGATGAGCTGAGCGCCCAGGCAGAAGTGAAGAAGCATCAGGTACTAG AACAAGCCCTTGCTGACTATGCCCAGACCATCAAACAACTAGCAGCCAGCAGTCAAGATATGATTGACCA TGAACATCCAGAGAGCACAAGGTTAACAATACGCCAAGCCCAGGTGGACAAGCTGTATGCCGGCCTAAAG GAGCTGGCAGGAGAGCGGCGTGAGCGTCTGCAGGAGCACCTCAGGCTGTGCCAGCTCCGCAGAGAGCTGG ATGACCTGGAGCAGTGGATACAGGAGCGAGAAGTCGTGGCAGCCTCCCATGAACTGGGCCAGGACTATGA GCATGTGACTATGCTTCGGGACAAATTCCGAGAGTTCTCCAGGGACACCAGCACCATTGGCCAAGAGCGT GTAGACAGTGCCAATGCCCTGGCCAATGGGCTCATTGCTGGGGGCCATGCTGCACGGGCCACCGTGGCCG AGTGGAAGGACAGTCTCAATGAGGCCTGGGCTGACCTGCTGGAGCTGCTGGACACAAGAGGTCAGGTGCT GGCTGCTGCTTATGAGCTGCAGCGCTTCCTGCATGGGGCCCGCCAAGCCCTGGCACGGGTGCAGCACAAG CAGCAGCAGCTTCCAGATGGGACGGGCCGCGACCTCAATGCTGCTGAGGCCCTGCAGCGCCGGCACTGCG CCTATGAGCACGACATCCAAGCCCTCAGCACTCAGGTCCAGCAGGTTCAGGACGATGGCCTCAGGCTACA AAAGGCCTATGCTGGAGACAAGGCTGAGGAAATTGGCCGTCACATGCAGGCAGTGGCTGAGGCGTGGGCC CAGCTCCAGGGAAGTTCTGCTGCCCGTCGCCAGCTGTTACTGGATACCACAGACAAATTCCGATTCTTCA AGGCTGTCCGGGAGTTGATGCTGTGGATGGATGGGATTAACCTGCAGATGGATGCCCAGGAGAGGCCCCG GGATGTGTCCTCTGCAGATTTAGTCATCAAAAACCAACAAGGCATCAAAGCAGAGATAGAGGCAAGAGCT GACAGGTTCTCCGCCTGCATTGACATGGGGCAAGAGCTGCTGGCCCGGAACCACTATGCCGCTGAGGAGA TCTCAGAGAAGCTGTCTCAGCTACAGTCCCGGCGCCAGGAGACAGCTGAAAAGTGGCAGGAGAAGATGGA CTGGCTACAGCTTGTTTTGGAGGTGCTTGTGTTTGGGAGAGATGCAGGCATGGCAGAGGCCTGGCTATGC AGTCAGGAGCCATTGGTGCGAAGTGCAGAACTGGGTTGCACTGTGGATGAAGTAGAGAGCCTCATCAAGC GGCATGAAGCCTTCCAGAAGTCAGCAGTGGCCTGGGAGGAGCGTTTCAGTGCCCTGGAGAAGCTCACTGC GCTGGAAGAGCGGGAGAATGAGCAGAAAAGGAAGAGGGAGGAGGAGGAACGAAGGAAACAGCCCCCTACT TCAGAGCCCATGGCTAGTCAACCGGAAGGGAGTCTGGTAGATGGCCAGAGAGTTCTTGACACTGCCTGGG ATGGGACCCAGTCAAAATTGCCACCATCCACACAAGCACCCAGCATTAATGGGGTCTGCACGGACACGGA GTCCTCACAGCCCCTGTTGGAACAGCAAAGACTTGAACAGAGCAATGTCCCAGAAGGGCCTGGATCTGGC ACAGGAGACGAGTCCAGCGGGCCCCGGGGAGAGAGGCAGACCCTGCCCCGGGGCCCTGCTCCGTCTCCAA TGCCCCAGAGCAGATCGTCTGAGTCAGCTCATGTTGCCACCCTGCCCGCACGAGGTGCTGAGCTCTCTGC TCAGGAACAGATGGAAGGGACGCTGTGCCGCAAACAGGAGATGGAAGCCTTCAATAAGAAAGCTGCCAAC AGGTCCTGGCAGAATGTGTACTGTGTACTTCGGCGTGGAAGCCTCGGCTTTTACAAGGATGCCAGGGCAG CTAGTGCAGGAGTGCCATACCATGGAGAAGTGCCTGTCAGTCTGGCCAGGGCCCAGGGCAGTGTGGCCTT TGATTATCGGAAACGCAAACATGTCTTCAAGCTGGGCTTGCAGGATGGGAAAGAGTATCTATTCCAGGCC AAGGATGAGGCAGAGATGAGCTCATGGCTGAGAGTGGTGAATGCAGCCATTGCCACTGCGTCCTCGGCCT CTGGAGAGCCAGAAGAGCCAGTGGTGCCCAGTGCCAGCCGGGGTCTGACCAGGGCCATGACCATGCCCCC AGTGTCACAGCCTGAGGGCTCCATCGTGCTTCGCAGCAAGGATGGCAGAGAAAGAGAGCGAGAAAAACGA TTCAGCTTCTTTAAGAAGAACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209033 representing NM_019167 Red=Cloning site Green=Tags(s) MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSH LARVTCRVGDLYSDLRDGRNLLRLLEVLSGETLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHD IVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLA FNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYF SKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSY RTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQ EKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAE LAAEHYHDIKRIAARQNNVARLWDFLREMVAARRERLLLNLELQKVFQDLLYFMDWMAEMKGRLQSQDLG KHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELA ATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLT LEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLV SSPEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQAR AGERARALEAALAFYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTA VSDIAEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKV IESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQTGWEDL RATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSE YSRLRTLGEEVTRDQADPQCLFLRQRLEALGTGWEGAGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSS QEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRH RKNQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANK DWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARSLFDANRAELFAQSCSALESWLE SLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKF RALCQPMKDRCRRLQASREQHQFHRDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGH EPRIADLKERQRTLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELH MMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIRQAQVDKLYAGLK ELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQER VDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHK QQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWA QLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEARA DRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLC SQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPT SEPMASQPEGSLVDGQRVLDTAWDGTQSKLPPSTQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSG TGDESSGPRGERQTLPRGPAPSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAAN RSWQNVYCVLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQA KDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSASRGLTRAMTMPPVSQPEGSIVLRSKDGREREREKR FSFFKKNK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019167
ORF Size	7164 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019167.1, NP_062040.1
RefSeq Size	8178 bp
RefSeq ORF	7167 bp
Locus ID	29211
MW	270.9 kDa
Gene Summary	may modulate the structural integrity of the Golgi apparatus; binds preferentially to Arp1 rather than actin to mediate the direct interaction of dynein and dynactin with vesicles [RGD, Feb 2006]

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