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Usp28 (NM_001108144) Rat Tagged ORF Clone
CAT＃: RR208788
- TrueORF®
Usp28 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 28 (Usp28), (10 ug)
"NM_001108144" in other vectors (3)
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CNY 17,290.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208788 representing NM_001108144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCGGAGCTGCAGCAGGACGACGCGGCGGGGGCGGCGGACGGCCATGGCTCGAGCTGCCAGATGC TGTTAAATCAGCTGCGAGAAATCACGGGCATCCAGGATCCTTCCTTTCTTCATGAAGCATTAAAGGCCAG TAATGGTGACATCACCCAGGCTGTCAGCCTTCTCACAGACCAAAGAGTTAAGGAGCCCAGTCACGACACA GCTGCTACAGAACCATCTGAAGTAGAAGAGAGTGCTACCAGCAAAGATCTCTTAGCAAAAGTGATAGACC TTACACATGACAACAAAGATGACCTTCAGGCAGCCATTGCCTTGAGTCTCCTGGAGTCTCCTAACATTCA GACTGACAGCAGAGATCTTAATAGAATACATGAAGCTAACTCTGCAGAGACTAAACGATCAAAGAGAAAA CGCTGTGAAGTTTGGGGAGAAAACCACAATCCCAATAACTGGAGGAGAGTGGATGGTTGGCCAGTTGGGC TGAAAAATGTTGGCAACACATGTTGGTTCAGTGCTGTTATTCAGTTGCCTGAATTTCGAAGACTTGTTCT CAGTTACAGCCTGCCACAGAACATTCTTGAAAATTGTCGAAGTCACACTGAAAAAAGAAATATCATGTTT ATGCAAGAGCTTCAGTATTTATTTGCTCTGTTATTGGGATCAAATCGGAAATTTGTAGATCCTTCTGCTG CCCTGGATCTACTGAAGGGGGCCTTCCGATCCTCAGAGGAGCAACAGCAAGATGTGAGTGAATTCACACA CAAGCTCCTGGATTGGCTGGAGGATGCGTTCCAGCTAGCTGTTAATGTTAACAGCAATCCCAGGACTAAA TCTGAAAACCCCATGGTGCAGCTGTTCTACGGTACCTTCCTGACAGAAGGGGTCCGAGAAGGAAAACCCT TTTGTAACAACGAGACCTTCGGCCAGTACCCTCTTCAGGTAAACGGTTACCACAACTTAGACGAGTGTTT GGAAGGGGCCATGGTGGAAGGTGACATTGCGCTGCTTCCTTCTGATCGCTCAGTGAAATACGGACAAGAG CGTTGGTTTACAAAGCTGCCTCCAGTGTTGACCTTCGAACTCTCCAGATTTGAGTTTAATCAGTCTCTTG GGCAGCCTGAGAAAATTCATAATAAGCTGGAATTTCCTCAGGTTATTTATATGGATAGGTACATGTACAA AAGCAAAGAGCTTATCCGAAGTAAGAGAGAGAGCATTCGAAAGTTGAAGGAGGAAATACAAGTTCTGCAG CAGAAATTAGAAAGGTATGTGAAGTACGGCTCCGGTCCATCTCGCTTCCCACTCCCCGACATGTTGAAAT ACGTTATTGAATTTGCCAGTACAAAACCTGCCTCTGAAAGCTGTCTGTCTGGAAGTGTTGAGCATATGAC GTTACCACTTCCTTCTGTGCACTGCCCGATTTCTGACCTCACAGCCAAGGAAAGTTCAAGTCCCAAAAGC TGCTCTCAGAATGCTGAGGGTTCCTTTTCCTCTCCGGAAGATGCTCTACCCAACTCTGAGGTAATGAATG GGCCATTTACTTCTCCTCACTCTTCCCTGGAAATGCCTGCACCCCCACCAGCTCCTCGGACAGTCACAGA TGAGGAAATGAATTTCGTTAAGACCTGTCTTCAGAGGTGGCGGAGTGAAATTGAACAGGATATACAAGAC TTAAAGAATTGTATCTCGAGCACCACCCAGGCTATTGAGCAGATGTACTGTGATCCTCTTCTTCGTCAGG TGCCTTATCGCTTACATGCAGTTCTTGTTCATGAAGGACAAGCCAGTGCAGGGCACTACTGGGCGTACAT CTATAACCAGCCCCGGCAGATCTGGCTCAAATACAACGACATCTCTGTCACTGAGTCTTCCTGGGAAGAA CTTGAAAGAGATTCTTACGGGGGCCTGAGAAATGTCAGCGCATACTGCTTGATGTATATCAATGACAAGT TGCCCCACTGCAGTGCAGAGGCAGCGCATGGTGAATCTGATCAGACTGCAGAAGTGGAAGCCCTGTCTGT GGAACTCAGGCAGTACATTCAGGAAGATAACTGGAGGTTTGAGCAAGAGGTGGAGGAATGGGAGGAAGAA CAGTCCTGTAAGATCCCACAGATGGAGTCCTCCCCAAACTCTTCCTCACAGGATTTTTCCACATCACAAG AGTCTTCAGCAGCGTCCTCGCATGGCGTTCGATGTTTGTCTTCTGAACATGCTGTGATTGCAAAGGAGCA GACTGCCCAGGCCATCGCAAACACGGCTCATGCCTATGAGAAGAGCGGGGTAGAAGCAGCCTTGAGTGAG GTGATGCTGAGCCCTGCCATGCAAGGGGTCCTCCTGGCCATAGCTAAGGCGCGCCAGACCTTTGACCGAG ACGGGTCTGAAGCAGGGCTTATTAAGGCATTTCATGAAGAGTACTCCAGGCTCTATCAGCTTGCCAAAGA GACACCCACCTCTCACAGTGATCCTCGCCTTCAGCATGTTCTCGTCTACTTTTTCCAAAATGAAGCACCC AAGAGGGTAGTAGAACGAACCCTTCTGGAACAGTTTGCAGATAGAAACCTTAGCTATGATGAGAGATCTA TCAGTATCATGAAGGTGGCTCAGGCCAAACTGATGGAAATTGGTCCAGAGGACATGAACATGGAAGAGTA TAAGAGGTGGCATGAAGACTATAGTCTGTTCCGAAAGGTGTCTGTGTACCTGCTAACGGGCCTGGAACTC TTCCAAAAAGGAAAGTACCAGGAAGCTTTGTCCTACCTGGTGTACGCGTACCAGAGCAATGCTGGCCTGC TGGTGAAGGGGCCCCGCAGGGGCGTGAAGGAGTCTGTGATTGCTTTGTACCGCAGAAAATGCCTTCTGGA GTTGAATGCCAAGGCTGCTTCTCTTTTTGAAACAAATGACGACCATTCTGTAACAGAGGGCATTAATGTG ATGAATGAGCTGATCATTCCCTGCATCCACCTGATCATTAATAATGACATTTCCAAGGACGACCTTGATG CCATCGAGGTCATGAGGAACCACTGGTGCTCTTACCTGGGGAAGGACATCGCAGAAAATCTGCAGCTGTG TTTAGGGGAGTTCCTACCCAGGCTTCTAGATCCTTCCGCAGAAATCATCGTCCTAAAGGAGCCTCCAACT ATTCGACCGAATTCTCCCTATGATCTTTGCAGCCGGTTTGCAGCTGTCATGGAGTCCATTCAAGGAGTTT CAACAGTAACAGTGAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR208788 representing NM_001108144
Red=Cloning site Green=Tags(s) MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDT AATEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQTDSRDLNRIHEANSAETKRSKRK RCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQLPEFRRLVLSYSLPQNILENCRSHTEKRNIMF MQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSNPRTK SENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVKYGQE RWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQVIYMDRYMYKSKELIRSKRESIRKLKEEIQVLQ QKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSVEHMTLPLPSVHCPISDLTAKESSSPKS CSQNAEGSFSSPEDALPNSEVMNGPFTSPHSSLEMPAPPPAPRTVTDEEMNFVKTCLQRWRSEIEQDIQD LKNCISSTTQAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQIWLKYNDISVTESSWEE LERDSYGGLRNVSAYCLMYINDKLPHCSAEAAHGESDQTAEVEALSVELRQYIQEDNWRFEQEVEEWEEE QSCKIPQMESSPNSSSQDFSTSQESSAASSHGVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEAALSE VMLSPAMQGVLLAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAP KRVVERTLLEQFADRNLSYDERSISIMKVAQAKLMEIGPEDMNMEEYKRWHEDYSLFRKVSVYLLTGLEL FQKGKYQEALSYLVYAYQSNAGLLVKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINV MNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPT IRPNSPYDLCSRFAAVMESIQGVSTVTVK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108144 |
| ORF Size | 3237 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108144.1, NP_001101614.1 |
| RefSeq Size | 4171 bp |
| RefSeq ORF | 3240 bp |
| Locus ID | 315639 |
| UniProt ID | D3ZJ96 |
| MW | 122.2 kDa |
| Gene Summary | Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208788 | Usp28 (untagged ORF) - Rat ubiquitin specific peptidase 28 (Usp28), (10 ug) |
CNY 17,290.00 |
|
| RR208788L3 | Lenti ORF clone of Usp28 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 28 (Usp28), (10 ug) |
CNY 18,910.00 |
|
| RR208788L4 | Lenti ORF clone of Usp28 (mGFP-tagged ORF) - Rat ubiquitin specific peptidase 28 (Usp28), (10 ug) |
CNY 20,710.00 |
