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Fer (NM_001106928) Rat Tagged ORF Clone
CAT＃: RR208624
- TrueORF®
Fert2 (Myc-DDK-tagged ORF) - Rat fer (fms/fps related) protein kinase, testis specific 2 (Fert2), (10 ug)
"NM_001106928" in other vectors (3)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fert2; Flk; Flk_retired |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208624 representing NM_001106928
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGAGTGTGGAGTGTCCCCACTGTGAGGGTGACCTGGAGCCTGACTCTGACTCCAAGTTCTGTA AAAAATGCTCTATACCGATGAGCCCTGGGCCTTCCTCATCAGAGATCCAGAGATACCAGGAAAGAAAGGA GAGGCTATCCAAATTTGAGTCTATTCGTCATTCAATTGCTGGAATAATTAAGTCTCCAAAGTCGGTCCTC GGCTCTTCAACGCTCTCTGACGTGATCTCTGTGGGTGAGAAGCCCCTGGCGGAGCATGACTGGTACCACG GTGCCATCCCCAGGATAGAGGCACAAGAACTGCTGAAGCAGCAGGGAGACTTCCTGGTGCGAGAGAGCCA TGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGACGGACAAAGGAGGCACTTTATCATACAGTTT GTTGATAATCTATATCGATTTGAGGGCACCGGCTTTTCAAATATCCCTCAACTTATAGATCACCACTTCA ATACAAAGCAAGTCATCACCAAGAAGTCGGGGGTGGTTCTGCTGAATCCTATTCCAAAGGATAAGAAATG GGTTCTCAATCATGAAGATGTTTCATTGGGAGAATTACTGGGCAAGGGGAATTTTGGTGAGGTGTACAAG GGCACACTGAAGGATAAAACCCCTGTTGCTGTTAAAACGTGCAAGGAAGACCTGCCTCAGGAACTAAAAA TAAAGTTTTTACAGGAAGCCAAAATTCTCAAGCAATATGATCACCCCAATATTGTCAAACTTATAGGCGT GTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTCCCAGGAGGTGATTTTCTGTCCTTTCTG AGGAAGAGGAAGGACGAGCTAAAGTTGAAGCAGTTGGTGAGGTTTTCCTTGGACGTTGCTGCTGGCATGC TGTATCTCGAGGGCAAGATCTGCATTCACAGGGACCTGGCTGCACGGAACTGCCTGGTAGGTGAAAATAA TACTCTGAAAATCAGTGACTTTGGGATGTCTCGGCAAGAAGACGGTGGAGTGTATTCATCTTCTGGCTTA AAGCAGATTCCCATTAAGTGGACAGCACCAGAAGCACTGAATTATGGGAGATACAGCTCTGAAAGTGACG TGTGGAGCTTCGGCATCCTGCTCTGGGAGACTTTCAGCCTGGGAGTCTGTCCGTACCCTGGGATGACAAA CCAGCAAGCACGGGAACAAGTGGAGAGAGGGTATCGGATGTCAGCCCCACAGAACTGTCCCGAGGAAATT TTTACAATCATGATGAAGTGCTGGGATTACAAGCCTGAAAACCGCCCTAAGTTCAGTGACCTTCACAAAG AGCTCACGGCCATCAAGAAGAAAATCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208624 representing NM_001106928
Red=Cloning site Green=Tags(s) MEQSVECPHCEGDLEPDSDSKFCKKCSIPMSPGPSSSEIQRYQERKERLSKFESIRHSIAGIIKSPKSVL GSSTLSDVISVGEKPLAEHDWYHGAIPRIEAQELLKQQGDFLVRESHGKPGEYVLSVYSDGQRRHFIIQF VDNLYRFEGTGFSNIPQLIDHHFNTKQVITKKSGVVLLNPIPKDKKWVLNHEDVSLGELLGKGNFGEVYK GTLKDKTPVAVKTCKEDLPQELKIKFLQEAKILKQYDHPNIVKLIGVCTQRQPVYIIMELVPGGDFLSFL RKRKDELKLKQLVRFSLDVAAGMLYLEGKICIHRDLAARNCLVGENNTLKISDFGMSRQEDGGVYSSSGL KQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLGVCPYPGMTNQQAREQVERGYRMSAPQNCPEEI FTIMMKCWDYKPENRPKFSDLHKELTAIKKKIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106928 |
| ORF Size | 1359 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106928.1, NP_001100398.1 |
| RefSeq Size | 3387 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 301737 |
| MW | 51.5 kDa |
| Gene Summary | Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this clearly depends on cell type and stimulus (By similarity). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208624 | Fert2 (untagged ORF) - Rat fer (fms/fps related) protein kinase, testis specific 2 (Fert2), (10 ug) |
CNY 5,800.00 |
|
| RR208624L3 | Lenti ORF clone of Fert2 (Myc-DDK-tagged ORF) - Rat fer (fms/fps related) protein kinase, testis specific 2 (Fert2), (10 ug) |
CNY 7,700.00 |
|
| RR208624L4 | Lenti ORF clone of Fert2 (mGFP-tagged ORF) - Rat fer (fms/fps related) protein kinase, testis specific 2 (Fert2), (10 ug) |
CNY 8,550.00 |
