Spsb2 (NM_001009660) Rat Tagged ORF Clone
CAT#: RR208532
- TrueORF®
Spsb2 (Myc-DDK-tagged ORF) - Rat splA/ryanodine receptor domain and SOCS box containing 2 (Spsb2), (10 ug)
"NM_001009660" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Grcc9 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208532 representing NM_001009660
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAGACGGCCCTGGCAAGGGGCAGCAGCAGCACCCCTAGCTCGCACGCTCTGTACTCTGACTTAT CTCCTCCCGAGGGCTTGGAGGAGCTCCTGTCCGCACCCCCTCCTGACCTAGGGGCCCAACGACACCACGG CTGGAACCCCAAGGATTGCTCCGAGAACATCGATGTCAAGGAAGGGGGACTGTGCTTTGAGCGGCGCCCT GTGGCCCAGAGCACTGATGGAGTCCGGGGAAAGAGGGGCTATTCGAGAGGCCTGCATGCCTGGGAGATCA GCTGGCCCCTGGAGCAAAGGGGAACACACGCCGTGGTGGGCGTGGCCACCGCCCTCGCCCCGCTGCAGGC TGACCACTACGCGGCGCTTTTGGGCAGCAACAGCGAGTCCTGGGGCTGGGATATTGGGAGGGGAAAATTG TATCATCAGAGTAAGGGCCTTGAGGCCCCCCAGTACCCAGCCGGACCTCAGGGTGAGCAGCTAGTGGTGC CAGAGAGACTGTTGGTGGTTCTGGACATGGAGGAGGGAACTCTGGGCTACTCTATTGGGGGCACGTACCT GGGACCAGCCTTCCGTGGACTGAAGGGGAGGACCCTTTATCCCTCTGTAAGTGCTGTTTGGGGACAGTGC CAGGTCCGCATCCGCTACCTGGGCGAAAGAAGAGCGGAGGAACCACAATCCCTTCTGCACCTGAGCCGCC TGTGTGTGCGCCATGCTCTGGGGGACACCCGGCTGGGTCAAATATCCAGTCTGCCATTGCCCCCTGCCAT GAAACGCTATCTACTCTACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208532 representing NM_001009660
Red=Cloning site Green=Tags(s) MGQTALARGSSSTPSSHALYSDLSPPEGLEELLSAPPPDLGAQRHHGWNPKDCSENIDVKEGGLCFERRP VAQSTDGVRGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQADHYAALLGSNSESWGWDIGRGKL YHQSKGLEAPQYPAGPQGEQLVVPERLLVVLDMEEGTLGYSIGGTYLGPAFRGLKGRTLYPSVSAVWGQC QVRIRYLGERRAEEPQSLLHLSRLCVRHALGDTRLGQISSLPLPPAMKRYLLYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001009660 |
ORF Size | 792 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001009660.1, NP_001009660.1 |
RefSeq Size | 1257 bp |
RefSeq ORF | 795 bp |
Locus ID | 297592 |
UniProt ID | Q5M877 |
MW | 28.8 kDa |
Gene Summary | Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (By similarity). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208532 | Spsb2 (untagged ORF) - Rat splA/ryanodine receptor domain and SOCS box containing 2 (Spsb2), (10 ug) |
CNY 3,990.00 |
|
RR208532L3 | Lenti ORF clone of Spsb2 (Myc-DDK-tagged ORF) - Rat splA/ryanodine receptor domain and SOCS box containing 2 (Spsb2), (10 ug) |
CNY 6,080.00 |
|
RR208532L4 | Lenti ORF clone of Spsb2 (mGFP-tagged ORF) - Rat splA/ryanodine receptor domain and SOCS box containing 2 (Spsb2), (10 ug) |
CNY 6,650.00 |