Nek8 (NM_001105804) Rat Tagged ORF Clone

CAT＃: RR208517

TrueORF^®

Nek8 (Myc-DDK-tagged ORF) - Rat NIMA (never in mitosis gene a)- related kinase 8 (Nek8), (10 ug)

Datasheet

SDS

Plasmid Map

"NM_001105804" in other vectors (3)

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CNY 8,930.00

货期*

5周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208517 representing NM_001105804 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGTACGAGCGGATCCGAGTGGTGGGAAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTTCGAA AGGCTGACCAGAAGCTGGTGATCATCAAGCAGATCCCAGTGGAACAGATGACCAAAGAGGAGAGGCAGGC GGCACAGAATGAGTGCCAGGTGCTCAAACTGCTCAACCACCCCAACGTCATTGAGTACTACGAGAACTTC CTGGAGGACAAGGCCCTCATGATCGCCATGGAATATGCACCAGGTGGCACCCTGGCTGAGTTCATCCAGA AGCGCTGCAACTCCCTGCTGGAGGAGGAGACGATCCTTCACTTCTTTGTGCAGATCCTGCTTGCGCTGCA TCATGTGCACACACACCTCATCCTGCATCGGGACCTCAAGACCCAGAACATCCTTCTTGACAAGCACCGC ATGGTCGTCAAGATCGGGGACTTTGGCATCTCTAAGATCCTTAGCAGCAAGAGCAAGGCCTACACGGTGG TGGGTACTCCATGCTACATCTCCCCTGAGCTATGTGAGGGCAAGCCCTACAACCAGAAGAGTGACATCTG GGCTCTGGGCTGTGTCTTATATGAGCTTGCCAGTCTCAAGAGGGCCTTTGAGGCAGCGAACTTGCCCGCC CTGGTGCTGAAGATCATGAGCGGCACCTTTGCACCCATCTCTGACCGTTATAGTCCTGAGCTACGCCAGC TTGTCCTGAGTCTGCTCAGCCTGGAACCTGCACAGCGGCCACCCCTCAGCCACATCATGGCTCAGCCCCT CTGCATCCGGGCCCTACTCAACATCCACACCGATGTGGGCAGCGTCCGCATGCGGAGGGCAGAGAAGTGC CTGACCCCAGGAACACCTATGGCCCCGGGCAGCACAGGGAGCAGGGCCACCAGTGCCCGATGCAGGGGTG TCCCCCGGGGACCTGTGAGACCGGCCATCCCGCCGCCTCTGTCTTCCGTCTACGCTTGGGGTGGTGGCCT TAGCATTCCCCTGAGGTTGCCGATGCCCAACACGGAGGTGGTCCAGGTGGCCGCAGGGCGCACACAGAAG GCTGGCGTTACACGCTCAGGACGCCTCATTCTGTGGGAGGCCCCACCCCTGGGAACTGGAGGCGGCACAC TCCTTCCGGGGGCAGTGGAGCTGCCTCAGCCCCAGTTTGTCTCTCGTTTCCTGGAGGGCCAGTCAGGCGT GACCATCAAGCATGTGGCCTGCGGGGACCTGTTCACTGCTTGCTTGACGGACAGAGGCATCATCATGACT TTTGGCAGCGGCAGCAACGGGTGTTTAGGTCACGGCAGCCTGACTGACATCAGCCAGCCCACCATTGTAG AGGCCCTGCTGGGGTATGAGATGGTGCAGGTGGCCTGTGGAGCCTCTCATGTGCTGGCCCTGTCCGCAGA TGGAGAGTTATTTGCCTGGGGCCGAGGAGATGGCGGTAGGCTGGGACTAGGCACCAGGGAGTCCCATAAC TGCCCTCAGCAGGTGCCTATGGTCCCAGGACAGGAAGCTCAGAGAGTTGTCTGTGGTATTGACTGTTCCA TGATCCTCACCTCACCAGGCAGGGTCCTGGCCTGTGGAAGTAACAGGTTCAACAAGCTAGGCTTGGACTG CCTGTCCCTGGAGGAGGAGCCTGTTCCCCACCAGCAAGTGGAGGAGGCCCTGAGCTTCACGCCGCTGGGT TCTGCACCTCTGGACCGGGAGACCCTGCTGTGCGTGGACCTTGGCACTGCTCATTCAGCTGCTGTGACTG CTTCCGGGGCCTGCTATACTTTTGGCAGCAATCAGCATGGGCAATTGGGCACCAGTTCCCGCCGGGTCAG CAGGGCACCCTGTCGGGTCCAAGGCCTAGAGGGCATCAAGATGGTGATGGTGGCCTGTGGGGATGCCTTC ACTGTGGCCATTGGGGCAGAGGGGGAAGTGTATTCTTGGGGCAAAGGAGCCCGGGGTCGCCTGGGAAGGA GGGATGAGGATGCCGGACTCCCTAGACCAGTGCAGCTGGATGAGACTCATCCTTACACGGTGACATCAGT GTCCTGCTGCCATGGGAACACTCTTTTGGCTGTTCGATCAGTCACAGATGAACCAGTTCCCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208517 representing NM_001105804 Red=Cloning site Green=Tags(s) MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENF LEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHR MVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPA LVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPLCIRALLNIHTDVGSVRMRRAEKC LTPGTPMAPGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSIPLRLPMPNTEVVQVAAGRTQK AGVTRSGRLILWEAPPLGTGGGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMT FGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVACGASHVLALSADGELFAWGRGDGGRLGLGTRESHN CPQQVPMVPGQEAQRVVCGIDCSMILTSPGRVLACGSNRFNKLGLDCLSLEEEPVPHQQVEEALSFTPLG SAPLDRETLLCVDLGTAHSAAVTASGACYTFGSNQHGQLGTSSRRVSRAPCRVQGLEGIKMVMVACGDAF TVAIGAEGEVYSWGKGARGRLGRRDEDAGLPRPVQLDETHPYTVTSVSCCHGNTLLAVRSVTDEPVPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001105804
ORF Size	2094 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105804.1, NP_001099274.1
RefSeq Size	2626 bp
RefSeq ORF	2097 bp
Locus ID	287473
UniProt ID	D3ZGQ5
MW	75.3 kDa
Gene Summary	Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RN208517	Nek8 (untagged ORF) - Rat NIMA (never in mitosis gene a)- related kinase 8 (Nek8), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,930.00
RR208517L3	Lenti ORF clone of Nek8 (Myc-DDK-tagged ORF) - Rat NIMA (never in mitosis gene a)- related kinase 8 (Nek8), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,740.00
RR208517L4	Lenti ORF clone of Nek8 (mGFP-tagged ORF) - Rat NIMA (never in mitosis gene a)- related kinase 8 (Nek8), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,880.00

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