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Sgpl1 (NM_173116) Rat Tagged ORF Clone
CAT＃: RR208430
- TrueORF®
Sgpl1 (Myc-DDK-tagged ORF) - Rat sphingosine-1-phosphate lyase 1 (Sgpl1), (10 ug)
"NM_173116" in other vectors (3)
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CNY 6,768.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | S1PL; Spgl1; spl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208430 representing NM_173116
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGTACCGACCTTCTCAAGTTGAAGGACTTCGAGCCTTATTTGGAGATTTTGGAAGCATATTCCA CAAAAGCCAAGAATTACGTGAATGGGTACTGCACCAAATACGAGCCCTGGCAGCTCATTGCGGGGAGTGT CCTGTGTACTCTGCTGGTAGTCTGGGTATATGAGCTTATCTTCCAGCCAGAGAGTTTATGGTCTCGGTTT AAAAATAAATTATTTAGGCTTATAAGGAAGATGCCATTTATTGGACGTAAGATACAACAGCAGCTTACCA AAGCCAAGAAGGATCTTGTCAAGAACATGCCATTCCTGAAGTTGGACAAAGATTATGTGAAAACCCTGCC TGCTCAGGGTCTGAGCACAGCTGAGGTTCTGGAGAGACTCAAGGAGTACAGCTCCATGGATGTCTTCTGG CAAGAAGGGAAAGCCTCAGGAGCTGTGTACAGTGGGGAGCCGAAGCTCACCGAGCTGCTGGTGCAGGCTT ACGGAGAATTCACGTGGAGCAATCCACTGCACCCAGATATCTTCCCCGGACTACGGAAGTTAGAGGCAGA AATCGTGAGGATGACTTGCTCCCTCTTCAATGGGGGGCCAGACTCCTGTGGATGTGTGACTTCTGGGGGA ACAGAAAGCATCCTAATGGCCTGCAAAGCATATCGGGACTTGGCCTTAGAGAAGGGGATCAAAACTCCAG AAATTGTGGCTCCTGAGAGTGCCCACGCCGCATTCGACAAAGCAGCTCATTATTTCGGAATGAAGATTGT ACGCGTTGCGCAGAAAAAGAACATGGAGGTGGATGTGCGGGCAATGAAAAGAGCCATCTCCAGGAACACA GCCATGCTGGTCTGTTCTGCCCCACAGTTTCCTCATGGTGTGATAGATCCTATCCCCGAAGTGGCCAAGC TGGCTGTCAAATATAAAATCCCATTCCATGTGGATGCTTGTCTTGGGGGCTTCCTCATTGTCTTCATGGA GAAAGCAGGGTACCCACTGGAGAAACCATTTGATTTCCGGGTGAAAGGTGTGACCAGCATTTCAGCAGAT ACTCATAAGTACGGCTATGCTCCCAAAGGTTCATCAGTGGTGATGTACTCTAACGAGAAGTACAGGAAGT ACCAGTTCTTTGTTGATGCAGATTGGCAAGGTGGCATCTACGCATCTCCAAGCATAGCTGGCTCACGGCC TGGTGGCATCATTGCAGCCTGTTGGGCCGCCTTGATGCACTTTGGTGAGAACGGCTATGTTGAAGCTACC AAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTGGAAAACATCAAAAACATCTTCATTTTGG GGGATCCTCAATTGTCAGTTATTGCTCTGGGCTCCAACGATTTTGACATCTACCGACTATCTAATATGAT GTCTGCTAAGGGGTGGAATTTTAACTACTTGCAGTTCCCAAGAAGCATTCATTTCTGCATTACGTTAGTA CATACTCGGAAGCGAGTGGCAATCCAGTTCCTAAAGGATATCCGGGAATCGGTCACACAAATCATGAAGA ATCCTAAAGCTAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGGCAACCATTGACAGGAAGAT GGTTGCAGAAATATCCTCCGTCTTCTTGGACAGCCTTTATAGTACAGACCCTGTGACTCAGGGTAACCAG ATGAACGGTTCTCCAAAGCCCCGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR208430 representing NM_173116
Red=Cloning site Green=Tags(s) MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRF KNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFW QEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGG TESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNT AMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISAD THKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEAT KQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLV HTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQ MNGSPKPR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173116 |
| ORF Size | 1704 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173116.1, NP_775139.1 |
| RefSeq Size | 2033 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 286896 |
| UniProt ID | Q8CHN6 |
| MW | 63.8 kDa |
| Gene Summary | catalyzes the conversion of phosphorylated long chain bases into ethanolamine phosphate and a fatty aldehyde in sphingolipid catabolism [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208430 | Sgpl1 (untagged ORF) - Rat sphingosine-1-phosphate lyase 1 (Sgpl1), (10 ug) |
CNY 6,352.00 |
|
| RR208430L3 | Lenti ORF clone of Sgpl1 (Myc-DDK-tagged ORF) - Rat sphingosine-1-phosphate lyase 1 (Sgpl1), (10 ug) |
CNY 9,220.00 |
|
| RR208430L4 | Lenti ORF clone of Sgpl1 (mGFP-tagged ORF) - Rat sphingosine-1-phosphate lyase 1 (Sgpl1), (10 ug) |
CNY 10,070.00 |
