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Tbx6 (NM_001108920) Rat Tagged ORF Clone
CAT＃: RR208413
- TrueORF®
Tbx6 (Myc-DDK-tagged ORF) - Rat T-box 6 (Tbx6), (10 ug)
"NM_001108920" in other vectors (3)
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CNY 5,610.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208413 representing NM_001108920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCATCCACGAGAGTTATACCCCTCTCTGGGGACTGGCTACCGTCTGGGACACCCCCAGCCTGGGG CAGACTCAACCTTCCCACCTGCCCTGACAGAGGGCTACCGCTACCCTGATTTGGATACTTCTAAACTGGA TTGCTTCCTCTCTGGGATTGAGGCAGCTCCCCACACTCTGGCTGCACCCCCTCCTTTGCCCCTTCTCCCA TCTGCTCTGGGCCCTGAGACAGCACCGCCACCCCCAGAGGCCCTTCACTCGCTTCCTGGGGTCAGCCTGA GCTTGGAGAACCAGGAACTGTGGAAGGAATTCAGCGCTGTGGGAACAGAGATGATCATCACCAAGGCTGG CAGGCGTATGTTCCCTGCTTGCCGAGTATCAGTCACAGGCCTGGACCCGGAGGCCCGGTACTTGTTCCTT CTGGATGTGGTTCCAGTGGATGGGGCCCGATACCGCTGGCAGGGCCAGCACTGGGAGCCCAGTGGCAAGG CTGAGCCCCGCCTACCCGACCGTGTCTACATTCACCCTGATTCTCCTGCCACTGGTGCCCACTGGATGCG GCAGCCCGTATCCTTCCACCGTGTTAAGCTCACCAACAGCACGCTGGACCCCCATGGCCACCTGATCTTG CATTCCATGCACAAGTACCAGCCTCGCATTCACCTGGTGAGAGCCACCCAACTCTGCAGCCAACACTGGG GGGGTGTGGCCTCCTTCCGATTTCCTGAGACCACATTCATCTCAGTGACAGCCTACCAGAACCCTAGGAT CACACAGCTGAAGATCGCAGCCAATCCCTTTGCCAAAGGTTTCCGAGAAAATGGCAGAAACTGTAAGAGG GAGCGGGATGCCCGTGTGAAGAGGAAACTCCGGGGCCCAGAGCCAGTGGCCACAGAGGCCTGTGGGAGTG GGGATACACCAGGGGGTCCCTGTGACTCCACCCTGGGTGGGGATATTCGAGATTCAGATCCAGAGCCGGC TCCTACCCCCCAGGAAGCTGCTTCTGCCTCAGCTCCTCCATGTGGGGGCCCCAGTGCTGAGGCCTACCTC CTGCACCCTGCAGCTTTTCATGGGGCCCCCAGTCATCTACCAGCCAGGACCCCCAGCTTCCCTGAGGCTC CAGACCCTGGGCGCCCAGCCCCCTACTCAGCTGCATTCCTGGAGCTACAGCCTGGACCGGGGAGCTCTGC TTATCAAACGACCCCATCTGTAACACCCTTTGCCCCTCACTTTATTCAAGGGGCCCCCTTCCCTCTACCA TACCCAGGACCTGGAGGATATCTGGACATGGGATCCAAGCAAATGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208413 representing NM_001108920
Red=Cloning site Green=Tags(s) MYHPRELYPSLGTGYRLGHPQPGADSTFPPALTEGYRYPDLDTSKLDCFLSGIEAAPHTLAAPPPLPLLP SALGPETAPPPPEALHSLPGVSLSLENQELWKEFSAVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFL LDVVPVDGARYRWQGQHWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLIL HSMHKYQPRIHLVRATQLCSQHWGGVASFRFPETTFISVTAYQNPRITQLKIAANPFAKGFRENGRNCKR ERDARVKRKLRGPEPVATEACGSGDTPGGPCDSTLGGDIRDSDPEPAPTPQEAASASAPPCGGPSAEAYL LHPAAFHGAPSHLPARTPSFPEAPDPGRPAPYSAAFLELQPGPGSSAYQTTPSVTPFAPHFIQGAPFPLP YPGPGGYLDMGSKQMY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108920 |
| ORF Size | 1308 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108920.1, NP_001102390.1 |
| RefSeq Size | 1726 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 365371 |
| UniProt ID | D3ZJK7 |
| MW | 47.2 kDa |
| Gene Summary | T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to also play an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208413 | Tbx6 (untagged ORF) - Rat T-box 6 (Tbx6), (10 ug) |
CNY 5,610.00 |
|
| RR208413L3 | Lenti ORF clone of Tbx6 (Myc-DDK-tagged ORF) - Rat T-box 6 (Tbx6), (10 ug) |
CNY 7,510.00 |
|
| RR208413L4 | Lenti ORF clone of Tbx6 (mGFP-tagged ORF) - Rat T-box 6 (Tbx6), (10 ug) |
CNY 8,270.00 |
