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Sacm1l (NM_053798) Rat Tagged ORF Clone
CAT＃: RR208191
- TrueORF®
Sacm1l (Myc-DDK-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug)
"NM_053798" in other vectors (3)
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CNY 4,368.00
CNY 7,510.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Sac1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208191 representing NM_053798
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCACAGCCTACGAGCATCTGAAGCTGCATATTACACCTGAAAAGTTTTATGTGGAAGCTTGTG ATGATGGAGCAGATGATGTACTCATCATTGACCGCGTGTCTACAGAGGTCACCCTTGCAGTCAAAAAAGA TGTTCCCCCTTCTGCTGTTACAAGACCAATATATGGGATAATGGGCACAATCCATCTGGTGGCAGGCAAT TATCTTGTTGTCATTACCAAAAAGATGAAAGTAGGTGAATTTTTTAATCATGTAATCTGGAAAGCCACAG ATTTTGATGTTCTTTCTTATAAGAAGACAATGTTACACTTAACTGATATTCAGTTACAAGATAATAAAAC TTTCCTAGCAATGCTAAACCATGTCTTGAGTACGGATGGATTTTACTTTTCAACAACATATGACTTGACC CATACTTTGCAACGGCTATCTAACACGAGTCCAGAATTCCAAGAAATGAGTCTCCTAGAAAGGGCAGATC AGCGGTTTGTATGGAACGGGCATCTGCTCAGAGAACTGTCTGCTCAGCCAGAGGTCCATCGGTTTGCTCT TCCTGTGTTGCATGGCTTTATTACTATGCATTCATGTTCTATTAACGGGAAATATTTTGATTGGATTCTC ATCTCCCGGAGGAGTTGTTTCAGAGCCGGTGTGCGTTATTATGTGAGAGGAATTGATTCTGAAGGCCACG CAGCTAACTTTGTAGAAACAGAACAGATTGTGCACTACAGTGGGAACAGGGCTTCATTTGTACAGACTCG AGGATCAATACCTGTTTTCTGGTCACAAAGACCAAATCTAAAGTACAAACCAGATCCACAGATCAACAAA GTAGCAAATCACATGGATGGTTTCCAAAGGCATTTTGATTCACAAGTAATTATTTATGGAAAACAAGTAA TAATTAATCTGGTGAACCATAAGGGATCAGAGAAACCACTTGAGCAAACATTTGCAAAAATGGTGTCATC CTTGGGAAGTGGGATGATCAGATATATTGCCTTTGACTTCCATAAGGAGTGTAAAAATATGAGATGGGAT CGACTGAGTATTTTATTGGATCAAGTAGCAGAAATGCAGGATGAATTAAGTTATTTTTTAGTAGACTCTG CTGGCAAGGTGGTGACAAACCAGGAAGGAGTTTTCCGCAGCAACTGTATGGATTGTCTAGACAGAACGAA TGTGATACAGAGTTTATTAGCTCGGCGTTCCCTTCAGGCACAGCTGCAGAGACTAGGAGTTTTGCATGTG GGACAAAAGCTTGAAGAACAAGATGAGTTTGAGAAGATTTACAAAAATGCCTGGGCCGATAATGCTAATG CTTGTGCCAAACAGTATGCAGGAACCGGTGCCTTGAAGACTGACTTTACAAGAACTGGAAAAAGAACTCA GTTGGGACTGGTAATGGATGGCTTCAACTCATTATTACGGTACTACAAGAACAACTTTTCTGATGGATTT AGACAAGATTCTATAGACTTATTTCTTGGAAACTACTCAGTGGATGAATTAGACTCTCACAGTCCTTTAA GTGTTCCAAGGGACTGGAAATTCCTGGCGTTGCCTATCATCATGGTTGTTGCCTTTTCAATGTGCATCAT CTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACACTGGCATATGTCCTCTTCTGGGGAGTTGCAAGC ATTGGAACATTTTTTATTATTCTTTACAATGGCAAAGATTTTGTTGATGCTCCCAGACTTGTACAGAAAG AAAAGATAGACAAAAGATAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208191 representing NM_053798
Red=Cloning site Green=Tags(s) MAATAYEHLKLHITPEKFYVEACDDGADDVLIIDRVSTEVTLAVKKDVPPSAVTRPIYGIMGTIHLVAGN YLVVITKKMKVGEFFNHVIWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLSTDGFYFSTTYDLT HTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWIL ISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPVFWSQRPNLKYKPDPQINK VANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFAKMVSSLGSGMIRYIAFDFHKECKNMRWD RLSILLDQVAEMQDELSYFLVDSAGKVVTNQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHV GQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLVMDGFNSLLRYYKNNFSDGF RQDSIDLFLGNYSVDELDSHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVAS IGTFFIILYNGKDFVDAPRLVQKEKIDKR* TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053798 |
| ORF Size | 1761 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053798.2, NP_446250.1 |
| RefSeq Size | 3370 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 116482 |
| UniProt ID | Q9ES21 |
| MW | 67 kDa |
| Gene Summary | This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
JNK3 Couples the Neuronal Stress Response to Inhibition of Secretory Trafficking
,Guang Yang, Xun Zhou, Jingyan Zhu, Rui Liu, Si Zhang, Ainsley Coquinco, Yongting Chen, Yanhua Wen, Luba Kojic, William Jia, and Max S. Cynader,
Sci. Signal., Jul 2013; 6: ra57.
,PubMed ID 23838184
[Sacm1l]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208191 | Sacm1l (untagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug) |
CNY 7,510.00 |
|
| RR208191L3 | Lenti ORF clone of Sacm1l (Myc-DDK-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug) |
CNY 9,410.00 |
|
| RR208191L4 | Lenti ORF clone of Sacm1l (mGFP-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug) |
CNY 10,260.00 |
