Ntn1 (NM_053731) Rat Tagged ORF Clone
CAT#: RR208030
- TrueORF®
Ntn1 (Myc-DDK-tagged ORF) - Rat netrin 1 (Ntn1), (10 ug)
"NM_053731" in other vectors (3)
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CNY 7,790.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | netrin-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208030 representing NM_053731
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCGCGCTGTGTGGGAGGCGCTGGCGGCGCTGGCGGCGGTGGCGTGCCTGGTGGGCGCGGTGCGCG GCGGGCCCGGGCTTAGCATGTTCGCCGGCCAGGCGGCGCAGCCTGATCCTTGCTCGGATGAGAACGGGCA CCCACGCCGCTGCATCCCGGACTTTGTCAACGCGGCTTTCGGCAAGGACGTGCGCGTGTCCAGCACCTGC GGCCGGCCCCCGGCGCGCTACTGCGTGGTGAGCGAGCGTGGCGAGGAGCGGCTGCGCTCCTGTCACCTCT GCAACTCTTCCGATCCCAAGAAAGCGCATCCGCCCGCCTTCCTCACCGACCTCAACAATCCGCACAACCT GACGTGCTGGCAGTCTGAGAACTACCTGCAGTTCCCGCACAACGTTACGCTCACTCTGTCGCTCGGCAAG AAGTTTGAGGTGACCTATGTGAGCCTGCAATTCTGCTCGCCCCGGCCAGAGTCCATGGCCATCTACAAGT CCATGGACTACGGGCGCACGTGGGTGCCCTTCCAATTCTATTCCACTCAGTGCCGCAAAATGTACAACCG GCCGCACCGCGCGCCCATCACTAAGCAGAACGAGCAGGAGGCGGTGTGCACCGACTCGCACACCGACATG CGCCCGCTCTCCGGCGGGCTGATCGCTTTCAGCACGCTGGACGGGCGGCCCTCGGCGCACGACTTCGACA ACTCGCCGGTGCTGCAGGACTGGGTCACCGCCACAGACATCCGCGTGGCTTTCAGCCGCCTGCACACGTT TGGCGACGAGAACGAAGATGACTCGGAGCTGGCTCGCGACTCCTATTACTATGCAGTATCTGACCTGCAG GTTGGCGGCCGCTGCAAGTGCAATGGCCACGCGGCGCGTTGCGTGCGCGACCGAGACGACAGTCTGGTGT GTGACTGTAGGCACAACACGGCCGGCCCTGAATGCGACCGTTGCAAGCCCTTCCACTACGATCGGCCCTG GCAGCGCGCCACGGCTCGCGAGGCCAACGAGTGCGTGGCCTGCAACTGCAACCTTCATGCTCGGCGCTGC AGATTCAACATGGAGCTTTACAAGCTATCAGGGCGCAAGAGCGGGGGTGTCTGTCTCAACTGCCGCCACA ACACTGCCGGCCGCCACTGCCACTACTGCAAGGAGGGCTTCTACCGAGACATGGGCAAGCCTATCACCCA CCGGAAGGCTTGCAAAGCCTGTGATTGCCACCCTGTGGGTGCTGCTGGTAAGACCTGCAATCAAACCACC GGCCAGTGTCCCTGCAAGGACGGCGTGACAGGCATCACCTGCAACCGCTGTGCCAAAGGCTACCAGCAGA GCCGCTCCCCCATCGCCCCTTGCATTAAGATTCCTGTAGCGCCACCCACCACTGCAGCCAGCAGCATGGA AGAACCTGAAGACTGTGACTCCTACTGCAAGGCTTCCAAAGGAAAACTGAAGATGAACATGAAGAAATAC TGCAGGAAGGACTATGCTGTCCAGATCCACATTCTGAAGGCGGACAAAGCAGGGGACTGGTGGAAGTTCA CAGTGAACATCATCTCCGTGTACAAGCAGGGCACAAGTCGTATACGCCGTGGTGACCAGAGTTTGTGGAT CCGTTCGCGAGACATCGCTTGCAAGTGTCCCAAAATCAAGCCCCTCAAGAAGTACCTGCTGCTGGGCAAT GCTGAGGACTCGCCTGACCAGAGTGGCATCGTGGCAGACAAGAGCAGCCTGGTGATCCAGTGGCGGGACA CATGGGCACGACGGCTGCGCAAGTTCCAGCAGCGGGAGAAGAAGGGCAAGTGCAAGAAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208030 representing NM_053731
Red=Cloning site Green=Tags(s) MMRAVWEALAALAAVACLVGAVRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTC GRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGK KFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDM RPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQ VGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRC RFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKTCNQTT GQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSMEEPEDCDSYCKASKGKLKMNMKKY CRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGN AEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053731 |
ORF Size | 1812 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053731.2, NP_446183.2 |
RefSeq Size | 1815 bp |
RefSeq ORF | 1815 bp |
Locus ID | 114523 |
MW | 67.8 kDa |
Gene Summary | plays a role in axon guidance during development; may also be involved in cell-cell interactions in the adult nervous system [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208030 | Ntn1 (untagged ORF) - Rat netrin 1 (Ntn1), (10 ug) |
CNY 7,790.00 |
|
RR208030L3 | Lenti ORF clone of Ntn1 (Myc-DDK-tagged ORF) - Rat netrin 1 (Ntn1), (10 ug) |
CNY 9,600.00 |
|
RR208030L4 | Lenti ORF clone of Ntn1 (mGFP-tagged ORF) - Rat netrin 1 (Ntn1), (10 ug) |
CNY 10,550.00 |