Lmo7 (NM_001001515) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207998 representing NM_001001515 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGATGGAGGACGCTGAGGCCGATTGCTCCATTGCATTCGCGGAGGCTCAGAGATGGGTGGAGG CTGTAACTGAGAAGAATTTTGAAACAAGGGATTTTCGAGCCTCTCTGGAAAATGGTGTTCTGTTGTGTGA TTTGATTAATAAGCTCAAACCCGGAGTCGTTAGGAAGATCAATAGACTGTCGACCCCGATAGCAGGTTTG GATAACATAAACGTTTTTTTGAAAGCTTGTGAACAGATTGGATTGAAAGAAGCCCAGCTTTTTCATCCCG GGGATCTACAGGATTTATCAAACCGAGTCACTGTCAAGCAAGAAGAGACTGACAGGAGACTGAAAAATGT TTTGATAACTTTATACTGGCTGGGAAGAAAAGCACAAAACAACCCCTACTATCATGGTCCCTATCTCAAT TTGAAGGCTTTTGAGACTCTTTTAGGACAAGCTTTGACTAAGGCACTTGAAGATTCTAGCTGCCTGAAGA GAAGTGGCCGGGACAGTGGCTACGGGGATATCTGGTGTCCGGACAGAGGAGACTTTCCTGCCCCTCCCGG GAGCCACAGGAGGGAAGATTCCTTTGAAAGCTTGGACTCCTTGGGGTCTAGATCACTGACCAGCTGCTCC TCAGACCTCACTCTCAGAGGCGGGAGGGAAGGTTGCGAAAGTGACAACGATTGGGAATTCACCTTCAAAA TGCAGGATTATAACAAAGATGACATGTCCTACCGACGGATTTCGGCCATTGAACCGAAGTCAGCTCTACC ATTCAATCGCTTCTTGCCTAACAAAAGCAAGCAGCCATCCTATGTGCCAGCGCCTTTGAGGAAGAAGAGG CCAGACAAAAATGAGGATAATAGAAGAAGCTGGGCTAGCCCTGTCTACACAGAAACAGAGGGAACATTTT CAAGTACCCAGAGGAGAGCTTGGGGTCCCAAGATGGAGAACTGGCACACAGTCCAAGAAACTTCCACCTC CTCTTGGTGTTTAGAAGAGGAAGAAGAAAAAGTCACACGCTTGCCCAATGTTGTGAAGGACGATCTCTAT GTGCGGAAGCTTAGCCCAGTCATGCCCAGCCCAGGGAGTTCTTTCGATCAGTTCCTTCCCAAAGGCTGGA CCCCTGAAGATATGAACTGGAAAAAAATAAAAAGAGAGACTTATAAGCCATGGTATAAGGAATTTCAGGG ACTCAGTCAGTTTTTATTGCTTCAGGCCCTCCAAACCTACTCTGATGATATCTTGTCTTCGGAGACAAAT GTCAAACTCGATCCTACCTCTGGCCCGAGGCTCATAACTCGCAGGAAGAACTTTTCTCCCACCCCAGGCT ACCGAGCCCACGACCTCGAGCCGCAATCCCTAGATCCTGACTTAGAAAATGACGATTTCTTTGTCAGAAA AACGAGGGCTTTCCATGCCAATCCGTGTGTTCTTCGAGCCTTCGAAGACTTTAGACGGTTCTCCGAGCCG GACGACGCTGTCGAACGCGATATAATTTTGCAGTGTCGGGAAGGTGAACTCGTACTTCCGGATTTAGAAA AGGATGACATGATTGTTCGCAGGATCCCAGCTCAGAAGAAAGAAGTGCCCCTTTCTGGGGCGCCAGATAG ATACCAGCCAGTCCCCTTCCCTGAACCGTGGACTCTACCTCCAGAAATCCAAGCGAAATTTCTCTCGGTG CTTGAAAGGACATGCCCACCCAAAGACAAACCAAGTAGCTGTAGGGTGTTAGTACCTTCCCATCGACTGA AGAAAGACGACATGTTGACTCGCAAGATCCAGTCTTGGAAGCTGGGGACTGCTGTGCCACCCATCAGCTT CAGGCCTGGCCCCTGCAGCGAGGCCGACCTACGGAAGTGGGAGGCCATCCGGGAGGCCAGCAGACTTAGG CACAGGAAGCGGCTGATGGTTGAGAGACTCTTTCAAAAGATTTATGGTGAGAACGGGAGCAAGTCCCTGA GCGATGTCAGCGCAGAGGATGTTCAGAGCCTGCGTCAGCTGCGCTACGAGGAGATGCAGAAAATAAAATC CCAGTTGAAGGAGCAAGATCAGAAATGGCAGGATGACCTTGCGAAATGGAAAGATCGCCGGAAGAGTTAC ACTTCAGACCTGCAGAAGAAAAAGGAGGAGAGGGAAGAAATTGAAAAGCAGGCCCTTGAGAAGTCTGATC GAAGCTCCAAGACATTCAGGGAAATGCTGCAGGACAGGGAATCTCAAAGTTCTACAACTACATCTAGAAG GAGGATCTATTCCTCTGATGACATCCTGGATGATGGCGTGCTCCCTCCTACAGTGACTCTGTCAGAAACT AGTTACCAGAGCGAGAGGCTAGAAGACAAGGCAAGAGCTCATCCTGCTGAGATGCCCAAGGAAGATTCCA CAACATTTGCAAGTAGAGAGGACGGTATAGCAGCCGAGATCCCGCTTGCTTCTCATATTCCTGAAGAGCG ACGTCAGGCCCCTTGGCCTTCTGCAGAAACACGCCAGGCCTCTTCATCTTCCGCAGAATCGCACCAGGCC TCCTTGCCTTCCACAGAATCAGACCAGGCCCGGTTGCCATCTGCAGAACCGCACCAGGCCTCTTTCTCTT CTGCAGAACCTCGCCAGGTCCCTTTGGTCTCTGCAGAACCACACCAGGCCTCTTCTTTCTCCTCCACAGA ACCGCACCAGGCCTCTTTGTCTTCTGCACTACAGCGTCCATCCTCACTCTCTTCTTCTCAGCAGTCATGG AGCACAAGAGGAGAGTCAACTCGGATTTCAGCTTCTCTCCCTAGAAGTTACCAGAGAAGTGATACAGCCA GGCTAACATCTGTGGTCACAGCGAGACCTTTTGGCACATCGTCACGGGGAATCTCATCACTGCCCAGATC CTACACGATGGATGATGGCTGGATATACAATGGAGATGTTGAAGTTGTCAAGAGAGAGCAACATGATTCG GGCAGAGCCGCCGGCATGAAGCCTGACTCCAGCCACTTTATTTTGGGCTCATCTAGTGAGAAGGAGGTAG CCACAGGAGAAGATGGGGCAAGCCTGTCCTCTTTCGTACTCCCTTCTTCCTCATCTCTCTCCCACGACCA GGCAGCCTCCTCAAAAGCCACATTCTCATCAACGTCTGGCCTGGATTCAGTGTCTGACTCTGGAGAAGGG AGAAGCTCATCACTGAGGGAAGTTTCAAGGTCCCCGGATCAGTTCAGCGATATGAGAATCAGCATAAACC AGACGCCCGGGACCAAACATGACTTTGGATTTACAATAAAATGGGATGTTTCTGGGATCTCTGTAGCATC AGTTGAAAAAGGTAGCCCAGCAGAATTCTCTCAGCTGCAGGTAGATGATGAAATTCTCGCCATCAATAAC ACCAAGTTTTCATATAAGGACACAAAAAAGTGGGAAGAAGCTATGGCAAATGCCCAAGAAACTGGAAACT TAGTGATGGATGTCAGGCGCTACGGAAAGTCCGGTTCACCCGAAACAAAGTGGATCGATACAACTTCAGG AATTTACAGCACAGAGAAGTCTTCAAGTCTATCAGTGACAACTGATTTCTCTGAAAGCCTTCAAAGTCCT TATTCTGAATCCAAAGAAATCAATGGAATTCATGAGGGGAGCAACGCCTTTGATTCAAAAGCATCAGAAT CCATTTCTTTGAAAAATTTAAAAAGGCGATCACAATTTTTTGAACAAGGAAGCTCTGATTCTGTGGCTCC TGATCTTCCGGTTCCAACCCTCAGTGCCCCGAGTCGCTGGGCTTGGGATCAAGAGGAGGAGAGACGGCGG CAGGAGCGGTGGCAGAAAGAGCAAGATCGCCTACTGCAGGAAAAATACCAACGTGAGCAGGAGAAGCTGA GGGAAGAGTGGCAGAGAGCTCAGCAGGAGGCGGAGAGAGAGAATTCCAAGTACCTGGATGAGGAGCTGAT GGTCCTAAATTCCAACAGCATTTCCCTGACCTCCCGGGAGTCTGTGACTGCCTCCTGGGAACCCAGCTGG AGTGAAGGGTCCAAGTCTCTGGACAGGGAAGGCACCCGAGCCGGAGATGAGGACCGGGGACAGCTGGAAG ACGATGCTGTGTATGAGGACCAAAGTCAGAAGCTGCAGGAAAGGCTCATGCTTGAGCATGAGAAGAAAAG GAAGGAGCAAGAGGCTCAGGAAGAGGAGCTCCGGAAGCAGCGGGAAGCTGAGGCTCAAGCTCGGGCTGAG GCAGAAGCCCAGGCCAGGGCCGAGGCTCAGGCCCGGGCAGAGGCCCAGGCCCAGGCCGAGGCTGAGGCTA GAAAACGAGCTGAAGCACAGAAGCTCCAGGCAGAGAGGGAACGAGAAACTTCGGTCAAAATATACCAGTA CCGGAGGCCTATTGATTCCTATGATCTACCAAAGAGAGAAGAAGAATCTTCAGGGCTGCTTCCAATTGAC AGGAGTAAATCAAGATCTACTACAGAGCTGAACGACCCCCTCATAGAGAAGAGTGGAAGCAGCAAATATT CAGATCGAATTGGGACCACTAGCTTTTCACAAAGGAGTTCTAAGAAGGATCAAGGCCCCTCGGAAGCGGA GTTGGAGAGACAGCAAATCCTTCAGGAAATGAGGAAGAAAACATCTCTCCACAATGACAACAGCTGGATC CGACAGCGCAGTGCCAGTGTAAACAAGGAGCCCATCTGCCTGACTGGGATCATGAGAAGAGGGGAGTCTC TGGACAATCTGGATTCCCAGCGGCCAGGATCTTGGAGGCAGTCCTCCTGGCACAGCCAGCCTGCAGGAGT CTACGCATCTTCCTCTGTTCAAGACTTTAGTCGCCTGCCACCACAGCTGTTGTCCACGTCAAACCGTGCC TACATGCGGAACCCATCCTCTGCGGTACCGCCTCTAGCTGGCTCAGTGAAGACCTCCCCGGGGTCTCCCT CTCCACGTAGCCATTCTCCTTCGATGTCACAGTCAGGCTCTCAGCTACGTAACAGGTCAGTGAGTGGGAA ACGTGTGTGCTCCTACTGTAATAGCATTCTGGGCAAAGGAGCCGCCATGATCATCGAGACCCTGGGTCTT TGTTATCATTTACATTGTTTTAAGTGTGTCTCCTGTGAGCGTGACCTCGGAGGCTCTTCCTCCGGAGCCG AAGTCAGAATCCGAAACCACCAACTGTACTGCAATGACTGCTATCTCCGATTCAAATCTGGCCGGCCAAC CGCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207998 representing NM_001001515 Red=Cloning site Green=Tags(s) MEGMEDAEADCSIAFAEAQRWVEAVTEKNFETRDFRASLENGVLLCDLINKLKPGVVRKINRLSTPIAGL DNINVFLKACEQIGLKEAQLFHPGDLQDLSNRVTVKQEETDRRLKNVLITLYWLGRKAQNNPYYHGPYLN LKAFETLLGQALTKALEDSSCLKRSGRDSGYGDIWCPDRGDFPAPPGSHRREDSFESLDSLGSRSLTSCS SDLTLRGGREGCESDNDWEFTFKMQDYNKDDMSYRRISAIEPKSALPFNRFLPNKSKQPSYVPAPLRKKR PDKNEDNRRSWASPVYTETEGTFSSTQRRAWGPKMENWHTVQETSTSSWCLEEEEEKVTRLPNVVKDDLY VRKLSPVMPSPGSSFDQFLPKGWTPEDMNWKKIKRETYKPWYKEFQGLSQFLLLQALQTYSDDILSSETN VKLDPTSGPRLITRRKNFSPTPGYRAHDLEPQSLDPDLENDDFFVRKTRAFHANPCVLRAFEDFRRFSEP DDAVERDIILQCREGELVLPDLEKDDMIVRRIPAQKKEVPLSGAPDRYQPVPFPEPWTLPPEIQAKFLSV LERTCPPKDKPSSCRVLVPSHRLKKDDMLTRKIQSWKLGTAVPPISFRPGPCSEADLRKWEAIREASRLR HRKRLMVERLFQKIYGENGSKSLSDVSAEDVQSLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSY TSDLQKKKEEREEIEKQALEKSDRSSKTFREMLQDRESQSSTTTSRRRIYSSDDILDDGVLPPTVTLSET SYQSERLEDKARAHPAEMPKEDSTTFASREDGIAAEIPLASHIPEERRQAPWPSAETRQASSSSAESHQA SLPSTESDQARLPSAEPHQASFSSAEPRQVPLVSAEPHQASSFSSTEPHQASLSSALQRPSSLSSSQQSW STRGESTRISASLPRSYQRSDTARLTSVVTARPFGTSSRGISSLPRSYTMDDGWIYNGDVEVVKREQHDS GRAAGMKPDSSHFILGSSSEKEVATGEDGASLSSFVLPSSSSLSHDQAASSKATFSSTSGLDSVSDSGEG RSSSLREVSRSPDQFSDMRISINQTPGTKHDFGFTIKWDVSGISVASVEKGSPAEFSQLQVDDEILAINN TKFSYKDTKKWEEAMANAQETGNLVMDVRRYGKSGSPETKWIDTTSGIYSTEKSSSLSVTTDFSESLQSP YSESKEINGIHEGSNAFDSKASESISLKNLKRRSQFFEQGSSDSVAPDLPVPTLSAPSRWAWDQEEERRR QERWQKEQDRLLQEKYQREQEKLREEWQRAQQEAERENSKYLDEELMVLNSNSISLTSRESVTASWEPSW SEGSKSLDREGTRAGDEDRGQLEDDAVYEDQSQKLQERLMLEHEKKRKEQEAQEEELRKQREAEAQARAE AEAQARAEAQARAEAQAQAEAEARKRAEAQKLQAERERETSVKIYQYRRPIDSYDLPKREEESSGLLPID RSKSRSTTELNDPLIEKSGSSKYSDRIGTTSFSQRSSKKDQGPSEAELERQQILQEMRKKTSLHNDNSWI RQRSASVNKEPICLTGIMRRGESLDNLDSQRPGSWRQSSWHSQPAGVYASSSVQDFSRLPPQLLSTSNRA YMRNPSSAVPPLAGSVKTSPGSPSPRSHSPSMSQSGSQLRNRSVSGKRVCSYCNSILGKGAAMIIETLGL CYHLHCFKCVSCERDLGGSSSGAEVRIRNHQLYCNDCYLRFKSGRPTAM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001001515
ORF Size	5187 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001001515.1, NP_001001515.1
RefSeq Size	5190 bp
RefSeq ORF	5190 bp
Locus ID	361084
MW	195.6 kDa
Gene Summary	binds afadin and alpha-actinin; may facilitate association of nectin and E-cadherin in cell-cell adhesion [RGD, Feb 2006]

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